Manoj Kumar

Manoj Kumar

PhD

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9
Publications
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280
Citations

Publications

Publications (9)
Article
Fusarium oxysporum f. sp ciceris, the causal organism of chickpea vascular wilt disease reduces yield up to 90% annually. Xylem plays a prominent role during Fusarium wilt. In resistant hosts, vascular wilt pathogens are presumably recognized by both extra and intracellular plant receptors that elicit defense responses in the xylem vessels. However...
Article
Pesticide application for disease management is a major action for crop protection from last seven decades. The repeated application of pesticide is the most important cause of the reduction in microbial population. Soil microorganisms play an important role in efficient acquisition and transportation of nutrients to plant. Pesticides leached in so...
Article
Two plant growth promoting rhizobacteria (PGPR) from our laboratory Pseudomonas putida NBRIRA and Bacillus amyloliquefaciens NBRISN13 with ability to tolerate abiotic stress along with multiple PGP traits like ACC deaminase activity, minerals solubilisation, hormones production, biofilm formation, siderophore activity. Earlier we have reported both...
Article
Full-text available
Embryogenesis in cotton is a difficult task due its genome dependency. We used 3 cotton cultivars (Khandwa-2, G. Cot. 10, and BC-68-2) and Coker-312 as control for regeneration. Efficient somatic embryogenesis was induced in agronomically important Indian cotton cultivars, Khandwa-2 and G. Cot. 10. For callusing in all the cultivars, different medi...
Article
Full-text available
Explants transformation through Agrobacterium, followed by in vitro regeneration is only method used for genetic improvement of cotton. The currently used method are based on somatic embryogenesis, therefore time consuming, genotype-dependent and expensive. We report here a genotype-independent method for genetic transformation of cotton. Embryonic...
Article
SiO2–BaO–ZnO–xB2O3–(10−x) Y2O3, (0≤x≤10) glasses are synthesized. The effect of Y2O3 on the structural and optical properties of glasses has been investigated using different characterization techniques. The results are discussed in light of non-bridging oxygens (NBO), optical basicity and heat-treatment of glasses. The band gap has been calculated...
Article
Full-text available
A protocol for induction and establishment of Agrobacterium rhizogenes mediated hairy root culture of Gossypium hirsutum was developed through infection with the A4 strain and co-cultivation on hormone-free semi-solid MS medium with B5 vitamins. It resulted in the emergence of hairy roots from the leaf explants, 21 days after infection. The transfo...
Article
Transgenic cotton lines were developed for high-level expression of a synthetic cry1EC gene from a wound inducible promoter. The tobacco pathogenesis related promoter PR-1a was modified by placing CaMV35S promoter on its upstream in reverse orientation. The resultant chimeric promoter CaMV35S(r)PR-1a expressed constitutively and was further up-regu...

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