Manoj Kumar

Chickpea is a crucial pulse crop worldwide, serving as a significant source of protein. It is predominantly cultivated in the Indian subcontinent, where diverse consumption patterns have driven increased production demand. An efficient regeneration protocol is required for genetic manipulation of chickpea. We have improvised the regeneration protoc...

Fusarium wilt is the most destructive soil-borne disease that poses a major threat to chickpea production. To comprehensively understand the interaction between chickpea and Fusarium oxysporum, the xylem-specific transcriptome analysis of wilt-resistant (WR315) and wilt-susceptible (JG62) genotypes at an early timepoint (4DPI) was investigated. Dif...

It is expected that, by 2050, the rapid rise in population and simultaneous urbanization shall deplete clean water supplies. Domestic wastewater (DWW) contains inorganic and organic components that can be harmful to aquatic organisms. Traditional remediation approaches (physical, chemical and biological) can be used on-site or off-site to purify po...

Fusarium oxysporum f. sp ciceris, the causal organism of chickpea vascular wilt disease reduces yield up to 90% annually. Xylem plays a prominent role during Fusarium wilt. In resistant hosts, vascular wilt pathogens are presumably recognized by both extra and intracellular plant receptors that elicit defense responses in the xylem vessels. However...

Chickpea (Cicer arietinum L.) is a pulse crop valued for its high protein content, grown in semi-arid tropics and Mediterranean regions. Its yield remains affected by biotic and abiotic stresses with drought alone being responsible for up to 50%annual loss of yield. Transcriptome analysis of a sensitive and a tolerant cultivar of chickpea has been...

Chickpea (Cicer arietinum L.) is a pulse crop valued for its high protein content, grown in semi-arid tropics and Mediterranean regions. Its yield remains affected by biotic and abiotic stresses with drought alone being responsible for up to 50% annual loss of yield. Transcriptome analysis of a sensitive and a tolerant cultivar of chickpea has been...

Plant defensins are mainly known for their antifungal activity. However, limited information is available regarding their function in abiotic stresses. In this study, a defensin gene, Ca-AFP, from Cicer arietinum, commonly known as chickpea, was cloned and transformed in Arabidopsis thaliana for its functional characterization under simulated water...

Supplementary Material

Drought tolerance is the ability to survive under water deficit condition. A significant alteration in gene expression related to various physiological, regulatory and molecular functions is reported in plants. Plant defensins better known for their antifungal activities also play role during drought. Defensins exhibit three-dimensional folding pat...

Chickpea is a highly nutritious grain legume crop, widely appreciated as a health food, especially in the Indian subcontinent. The major constraints on chickpea production are biotic (Helicoverpa, bruchid, aphid, ascochyta) and abiotic (drought, heat, salt, cold) stresses, which reduce the yield by up to 90%. Various strategies like conventional br...

Pesticide application for disease management is a major action for crop protection from last seven decades. The repeated application of pesticide is the most important cause of the reduction in microbial population. Soil microorganisms play an important role in efficient acquisition and transportation of nutrients to plant. Pesticides leached in so...

Production of transgenic pigeonpea is becoming increasingly important, but the methods currently employed in production and subsequent screening still requires improvement. Here, we describe Agrobacterium-mediated genetic transformation of pigeonpea with reporter uidA (gus) gene and selectable marker, neomycin phospho-transferase (nptII) gene. Hist...

The gram pod borer (Helicoverpa armigera Hubner) is the most serious insect pest of pigeonpea. It is highly susceptible to the insecticidal proteins of Bacillus thuringiensis (Bt). A codon-optimized chimeric Cry1Aabc gene of Bt driven by a constitutive promoter was introduced in pigeonpea (cv. Asha) to confer resistance against the insect. A total...

Two plant growth promoting rhizobacteria (PGPR) from our laboratory Pseudomonas putida NBRIRA and Bacillus amyloliquefaciens NBRISN13 with ability to tolerate abiotic stress along with multiple PGP traits like ACC deaminase activity, minerals solubilisation, hormones production, biofilm formation, siderophore activity. Earlier we have reported both...

Embryogenesis in cotton is a difficult task due its genome dependency. We used 3 cotton cultivars (Khandwa-2, G. Cot. 10, and BC-68-2) and Coker-312 as control for regeneration. Efficient somatic embryogenesis was induced in agronomically important Indian cotton cultivars, Khandwa-2 and G. Cot. 10. For callusing in all the cultivars, different medi...

Explants transformation through Agrobacterium, followed by in vitro regeneration is only method used for genetic improvement of cotton. The currently used method are based on somatic embryogenesis, therefore time consuming, genotype-dependent and expensive. We report here a genotype-independent method for genetic transformation of cotton. Embryonic...

SiO2–BaO–ZnO–xB2O3–(10−x) Y2O3, (0≤x≤10) glasses are synthesized. The effect of Y2O3 on the structural and optical properties of glasses has been investigated using different characterization techniques. The results are discussed in light of non-bridging oxygens (NBO), optical basicity and heat-treatment of glasses. The band gap has been calculated...

A protocol for induction and establishment of Agrobacterium rhizogenes mediated hairy root culture of Gossypium hirsutum was developed through infection with the A4 strain and co-cultivation on hormone-free semi-solid MS medium with B5 vitamins. It resulted in the emergence of hairy roots from the leaf explants, 21 days after infection. The transfo...

Transgenic cotton lines were developed for high-level expression of a synthetic cry1EC gene from a wound inducible promoter. The tobacco pathogenesis related promoter PR-1a was modified by placing CaMV35S promoter on its upstream in reverse orientation. The resultant chimeric promoter CaMV35S(r)PR-1a expressed constitutively and was further up-regu...