Manik Ghosh

Birla Institute of Technology, Mesra | BIT Mesra · Department of Pharmaceutical Sciences & Technology

Ethnopharmacological relevance: Ficus religiosa L. is one of the most relevant members of the family of Moraceae. It is the most sacred tree of South Asia, and it is used in traditional Ayurvedic and Unani medicine to cure respiratory disorders like cough, wheezing and asthma. Some studies were performed to investigate the anti-asthmatic potential...

For the first time, we developed and validated a highly sensitive, selective and rapid HPLC-ESI-MS-MS method for simultaneous quantification of metolazone (MET), losartan (LOS) and its metabolite losartan carboxylic acid (LCA) in rat plasma. After solid-phase extraction, the analytes and internal standard (irbesartan) were extracted from 100 µL pla...

A highly sensitive, accurate and robust LC-MS/MS method was developed and validated for determination of nimorazole (NMZ) in rat plasma using metronidazole (MNZ) as internal standard (IS). The analyte and IS were extracted from plasma by precipitating protein with acetonitrile and were chromatographed using an Agilent Poroshell 120, EC-C18 column....

Litsea polyantha Juss. (Lauraceae) has a long history of medicinal use among the traditional healers of Oraon and Munda community of Jharkhand. Powdered bark and roots are used for pains, bruises and contusions and for fractures in animals. The present study is carried out to identify the phytoconstituents present in L. polyantha Juss. bark extract...

The release of verapamil hydrochloride from tablets with Eudragit RLPO or Kollidon SR with different drug-to-polymer ratios were investigated with a view to develop twice-daily sustained-release dosage form by solid dispersion (SD) technique. The SDs containing Eudragit RLPO or Kollidon SR at drug-polymer ratios of 1:1, 1:2, and 1:3 with verapamil...

Natural products serve as an important drug candidate for the drug discovery pipeline. Most medications that are today prescribed as therapeutic agents found their origin in natural sources, of which plant-based sources are significant. It should be noted that natural products are not confined to plants only but has a vast area. Earlier, owing to i...

Background: Carissa carandas Linn. (family Apocynaceae) is a climbing shrub, with a height of 10 or 15 ft (3-5 m). It is found in Jharkhand and other states of India. It is commonly known as "Karunda" or "Jasmin flower Carissa. " The major requirement for its growth is full exposure to the sun. C. carandas blooms and fruits throughout the year. Obj...

its all about the qualitative analysis of the secondary metabolite present and the anti microbial activity of different extracts abtained by using gradient elution technique.

The current global epidemic of the novel coronavirus (SARS-CoV-2) has been labeled a global public health emergency since it is causing substantial morbidity and mortality on daily basis. We need to identify an effective medication against SARS-CoV-2 because of its fast dissemination and re-emergence. This research is being carried out as part of a...

Background: Laccifer lacca (Kerr) produces a mixture of polyhydroxy anthraquinones (laccaic acid) known as lac dye. Literature suggests that these laccaic acids have its structural resemblance with the anticancer drug Adriamycin (ADR). Hence, it may possess potential anticancer activity. Method: This study was designed to explore the in vitro anti...

Global competitions have made the pharmaceutical industry undergo numerous obstacles to meet up with the pace of drug discovery, growth and expansion. In order to effectively manufacture a quality product, statistical methods can be implemented for continuous improvement. Evolution in quality is accessed by determining the beneficial effects due to...

To develop a quick, simple and reproducible dual column high performance liquid chromatography (HPLC) method to determine the albendazole and its metabolites in rat plasma. Albendazole (ABZ), albendazole sulfoxide (ABZSO) and albendazole sulfone (ABZSO2) were analyzed in rat plasma by high performance liquid chromatography using UV-detector. Prepar...

Objectives: Olaparib is an orally active poly (ADP-ribose) PARP (polymerases) inhibitor known to destroy cancer cells with BRCA1 or BRCA2 deficiency. An authentic, fast, distinct, and reliable reverse phase-high performance liquid chromatography (RP-HPLC) method was developed and promptly validated in tablet formulations for olaparib estimation....

To develop a quick, simple and reproducible dual column high performance liquid chromatography (HPLC) method to determine the albendazole and its metabolites in rat plasma. Albendazole (ABZ), albendazole sulfoxide (ABZSO) and albendazole sulfone (ABZSO2) were analyzed in rat plasma by high performance liquid chromatography using UV-detector. Prepar...

Since time immemorial natural products have been a great source of medicine to mankind. The anti-viral activities from several ayurvedic herbal medicines (in the form of crude extract or fraction or isolated compounds) have been established but their effectiveness against coronavirus still needs to be explored. They can provide a rich resource of a...

In December 2019, the Coronavirus disease-2019 (COVID-19) virus emerged in Wuhan, China. The first resolved COVID-19 crystal structure (main protease) has been developed and various repurposing activities are in process. In this study, a knowledge gap in relation to COVID-19, with the previously known fatal Coronavirus (CoV) epidemics, Severe acute...

Background The Arisaema (Araceae) is a genus of approximately 180 perennial herbs widely distributed in the evergreen and deciduous forests. This genus (Arisaema) is used as medicinal agent since ancient times. Experimental investigations have shown promising positive coorelation with its folklore claim and this encourages us to report updated medi...

Infections caused by HSV-2 are a public health concern worldwide, and there is still a great demand for the discovery of novel anti-herpes virus agents effective against strains resistant to current antiviral agents. In this context, medicinal plants represent an alternative source of active compounds for developing efficient antiviral therapies. T...

Nateglinide (NAT) and Pioglitazone (PIO) are an antidiabetic drugs combination and currently under clinical trial in countries like Japan. In this study, an alternative, a simple, sensitive high-performance liquid chromatography method has been developed (limit of detection: 15 ng/mL and limit of quantification: 50 ng/mL) for simultaneous estimatio...

Laccifer lacca (Kerr) (LL) has widely been used as pigmenting, dying and coloring agent in chemical industry. Although, it has wide range of industrial applications, but unfortunately, due to lesser availability of data, it has been ignored. Keeping in mind, the broad application of Laccifer lacca (Kerr), we tried to report the in vitro anti-cancer...

Background: Pathogenic bacteria (Gram positive/Gram negative) are serving as a vital precursor for the development of infectious diseases in humans. Arisaema tortuosum (Wall.) Schott (ATWS), a famous folklore medicine of Asian region has documented for great medicinal values. Objective: To evaluate the ATWS extracts and their fractions (leaf and tu...

Green synthesis of gold nanoparticles (GNPs) has received substantial attention, because nanoparticles are produced in an eco-friendly way using biomolecules present in plant extracts in a single step reaction. This research article highlights GNPs obtained using shade-dried leaf extracts of Millettia pinnata (L.) with aqueous auric chloride (HAuCl...

Background: Lac is traditionally used as a dye because of its coloring properties and it is obtained from Laccifer lacca Kerr. Now a days, it is widely used in food and coloring industry. Purpose: To evaluate the antioxidant, antimicrobial activity of different extracts along with identification of active constituents present in it. Methods: Antiox...

Abstract: A simple, rapid, precise and accurate HPTLC method was developed and validated for the estimation of Nintedanib, a novel tyrosine kinase inhibitor used in idiopathic pulmonary fibrosis, in bulk drug. Chromatography was carried out using silica gel 60 F254 TLC plate and mobile phase Chloroform: Methanol in the ratio 7:3 v/v. The densitomet...

Introduction Aerva lanata (Linn) of family Amaranthaceae is an important and commonly used plant for its medicinal and pharmacological properties and proving the traditional uses of flowers of A. lanata Linn. Objective All extracts of A. lanata were further evaluated for antioxidant, antimicrobial, and antiurolithiatic potential to scientifically...

Background: In order to combat neurocysticercosis, effective and novel Fixed Dose Drug Combinations (FDC) of combination dosage regimens have been always under trial; with Albendazole (ABZ) and Praziquantel (PZQ) combination being one of them. Such outcome is basically dependent on the pharmacokinetic profiling of their metabolites such as Albendaz...

Ethnopharmacological relevance: Vachellia (Acacia) nilotica and other plants of this genus have been used in traditional medicine of Asian and African countries to treat many disorders, including sexually transmitted diseases, but few studies were performed to validate their anti-microbial and anti-viral activity against sexually transmitted infec...

A simple, sensitive, selective and reproducible method based on a reversed-phase chromatography was developed for the determination of Praziquantel in rat plasma using internal standard as Diazepam. Praziquantel & Diazepam was separated on a C18 column Enable (250mm × 4.6 mm, 5μm), with retention times of 6.4 & 8.5 min. Ultraviolet detection was se...

A very simplistic, selective, sensitive, and reproducible procedure based on a reversed-phase high-performance liquid chromatography was used and developed for the determination of praziquantel in rat plasma. For the separation of praziquantel from the internal standard, diazepam on an Enable, C18 column (250±4.6 mm, 5 μm particle size), with the r...

Background To date, efforts for the prevention and treatment of human respiratory syncytial virus (RSV) infection have been still vain, and there is no safe and effective clinical accepted vaccine. Arisaema genus has claimed for various traditional bioactivities, but scientific assessments are quite limited. Objective This encouraged us to carry o...

Background Globally, reactive oxygen species have served as an alarm predecessor toward pathogenesis of copious oxidative stress-related diseases. The researchers have turned their attention toward plant-derived herbal goods due to their promising therapeutic applications with minimal side effects. Arisaema tortuosum (Wall.) Schott (ATWS) is used i...

Molecular docking of natural chemical entities from Arisaema plant species as hepatitis virus inhibitors

Pyrethrin; derived from natural source (Chrysanthemum cinerariifolium)confirmedas a fundamental nucleusfor the development of pyrethroids which ultimatelyresulted inthe outstanding insecticidal activityby targeting the nervous systems ofinsects.To date, the binding and entry mechanism by which RSV infects respiratory epithelial cells isnotstill wel...

Ficus religiosa extracts have been used in traditional Indian medicine to treat sexually transmitted infections such as gonorrhea and genital ulcers. The aim of this study was to investigate the antiviral activity of F. religiosa extracts against herpes simplex virus type 2 (HSV-2), the main causative agent of genital ulcers and sores. Water and ch...

One new hydrogenated naphthol was isolated for the first time from methanolic extract of Aerva lanata (L.) Juss. flowers. The compound was elucidated as (2S.3R) 3-(3-hydroxy-3-methyl pent-4-en-1-yl)-2, 5, 5, 8a tetra-methyldecahydronaphthalene- 2-ol. The isolated compound (PC) was screened for anti-urolithiatic activity by ethylene glycol induced u...

NLSART, 2016 BIT Mesra

Pyrethroids have shown promising potential to induce apoptogenic signaling pathways in various cells. Therefore, present study on pyrethroids was designed to unlock better alternative agents against cancer disease. Different targets such as estrogen (PDB: 3ERT), androgens (PDB: 2PIT) & cervix (PDB: 3F81) cancer receptors were used in the study. Typ...

The sensation of pain is initiated in peripheral pain receptors (nociceptors) and its purpose is to draw attention to tissue damage. In order to test analgesic activity, it is obviously necessary to induce pain in the subject and then modify the response to, or perception of, this pain. Analgesic studies of the methanol (90% v/v) extract (MELP) of...

Discovery of drugs against viruses is always an attractive area of research. Plants are important natural source for discovery of drugs against viruses. Here in this article we targeted at two points. First the selection of a particular plant against viruses can be done more rationally by using the latest computer techniques i.e. docking studies of...

Natural antioxidants have gained lot of interest among consumers and the scientific community because epidemiological studies have indicated that frequent consumption of natural antioxidants is associated with reduction of cardiac and other disorders with no side effects. A number of studies are going on throughout the world to identify pharmacolog...

The present work is mainly based on the preparation and evaluation of emulgel loaded with nimorazole (NMZ), which is a hypoxic cell radiosensitizing agent. The emulgel formulations prepared were investigated for various tests such as Viscosity, Spreadibilty, Extrudability, Drug release, Globules size and Zeta potential. The invitro release studies...

Herbal medicines are in great demand over the world for primary health care because of their efficacy, safety and lesser side effects. Traditional use of medicines is recognized as a way to potential future medicines. Of the top 34 top marketed drugs for different diseases, 14 are natural compounds and natural products are main sources of anticance...

Deltamethrin, a well known type 2 synthetic pyrethroid insecticide, is a widespread environmental toxicant. It has potential to accumulate in body fluids and tissues due to its lipophilic characteristics. The immune system is among the most sensitive targets regarding toxicity of environmental pollutants. Various methods are available in the litera...

The monitoring of the plasmatic concentrations of cardiovascular drugs is crucial for understanding their pharmacokinetics and pharmacodynamics. A simple, sensitive, specific, and high-throughput liquid chromatography/tandem mass spectrometry (LC–MS/MS) method was developed and validated for the simultaneous estimation and pharmacokinetic study of...

Synthetic pyrethroids are widely used insecticides and their exposure act as precursor for various health hazards. The aim of study to comparatively evaluate the in-silico immunotoxic potential of type 1 & 2 pyrethroids on different CD4 (PDB: 1GC1) & CD8 (PDB: 1BQH) immune receptors. Based on molecular docking, type 2 (fluvalinate (1)) possesses mo...

Litsea polyantha Juss. (Lauraceae) also known as Litsea monopetala Roxb. is a small to medium sized evergreen tree distributed chiefly in tropical and subtropical Asia, Australia and the Pacific Islands. The bark of Litsea polyantha Juss. has a long history of medicinal use among the traditional healers of Oraon and Munda community of Jharkhand (In...

Discovery of drugs against viruses is always an attractive area of research. Plants are important natural source for discovery of drugs against viruses. Here in this article we targeted at two points. First the selection of a particular plant against viruses can be done more rationally by using the latest computer techniques i.e. docking studies of...

A simple, highly sensitive and rapid LC-MS/MS method has been developed and validated for the quantification of metolazone in rat plasma using irbesartan as internal standard (IS). After simple protein precipitation extraction by acetonitrile, the analyte and IS were extracted from 50 μL plasma sample on an Agilent Poroshell 120, EC- C18 (50 mm × 4...

Flavonoids and phenolic compounds from plants have been reported to have multiple biological effects. In the present communication, bioactive compounds from Litsea polyantha Juss. bark were studied. The total phenolic content was determined by using the Folin-Ciocalteu reagent and aluminum chloride colorimetric method was used for determination of...

Objectives: The present study was aimed at identifying, isolation, antioxidant activity and quantification of a marker compound obtained from M. koenigii leaves. Methods: HPTLC fingerprinting and in-vitro antioxidant activity was carried out for four isolated fractions given the trivial name fraction 1 (MF1), fraction 2 (MF2), fraction 3 (MF3) and...

Inductively Coupled Plasma - Optical Emission Spectrometry (ICP-OES) was used to estimate and evaluate the levels of heavy metals in vegetables collected from various sites of Ranchi city (tribal dominated population) followed by health risk assessment by determining Metal Pollution Index (MPI), Daily intake of metal (DIM) and Health Risk Index (HR...

The ionic liquid 1-allyl-3-methylimidazolium chloride was prepared in a fast and efficient way by solventless microwave irradiation of allyl chloride and N-methylimidazole in a sealed vessel.