Mangesh Bhide

• Assoc. Prof. DVM, PhD
• Principal Investigator at University of Veterinary Medicine in Košice

Lyme borreliosis (LB) is a multisystemic tick-borne disease that can affect many organs and have various clinical manifestations in dogs. We attempted to summarise various aspects of Lyme disease: i. e., pathogenesis, epidemiology, benefits and risks of diagnostic approaches, treatment options, and prevention in dogs. Several diagnostic bottlenecks...

The gastrointestinal tract is the largest and most complex component of the immune system. Each component influences the production and regulation of cytokines secreted by intestinal epithelial cells. The aim of this study was to see how the probiotic strain Limosilactobacillus reuteri L26 and its exopolysaccharide (EPS) affect porcine intestinal-e...

Kynurenines have immunomodulatory and neuroactive properties and can influence the central nervous system. Previous studies showed the involvement of the kynurenines in the pathogenesis and progression of neurodegenerative disease. In neurodegenerative disorders, including tauopathies, the tryptophan metabolism is shifted toward neurotoxic agents a...

Background and purpose Kynurenines have immunomodulatory and neuroactive properties and can influence the central nervous system. Previous studies showed the involvement of the kynurenines in the pathogenesis and progression of neurodegenerative disease. In neurodegenerative disorders, including tauopathies, the tryptophan metabolism is shifted tow...

The choroid plexus, located in the ventricular system of the central nervous system (CNS), obtains numerous roles critical for the proper development and operating of the CNS. The functions range from the best-known ones of the barrier and cerebrospinal fluid (CSF) producer, through participation in immune answer, 'nourishment, detoxification and r...

West Nile virus (WNV), re-emerging neurotropic flavivirus, can cross the blood–brain barrier (BBB) and cause fatal encephalitis and meningitis. Infection of the human brain microvascular endothelial cells (hBMECs), building blocks of the BBB, represents the pivotal step in neuroinvasion. Domain III (DIII) of the envelope (E) glycoprotein is a key r...

Coronavirus disease (COVID-19) caused by the SARS-CoV-2 virus has been affecting the world since the end of 2019. The severity of the disease can range from an asymptomatic or mild course to acute respiratory distress syndrome (ARDS) with respiratory failure, which may lead to death. Since the outbreak of the pandemic, scientists around the world h...

Streptococcus pneumoniae invades the CNS and triggers a strong cellular response. To date, signaling events that occur in the human brain microvascular endothelial cells (hBMECs), in response to pneumococci or its surface adhesins are not mapped comprehensively. We evaluated the response of hBMECs to the adhesion lipoprotein (a laminin binding prot...

Neisseria adhesin A (NadA), one of the surface adhesins of Neisseria meningitides (NM), interacts with several cell types including human brain microvascular endothelial cells (hBMECs) and play important role in the pathogenesis. Receptor binding pockets of NadA are localized on the globular head domain (A ³³ to K ⁶⁹ ) and the first coiled-coil dom...

Bacterial exopolysaccharides (EPSs) are known to modulate immunity. To date, a plethora of studies have reported the effect of EPSs on intestinal cells; however few works have revealed a complete picture of the signalling events in intestinal epithelial cells induced by bacterial EPSs. Here, using transcriptomics, we comprehensively mapped the biol...

Lyme borreliosis is one of the major tick-borne diseases in Europe. Events of the translocation of Borrelia across the blood-brain barrier (BBB) involve multiple interactions between borrelial surface proteins and receptors on the brain microvascular endothelial cells (hBMECs). In this study, we aimed to identify proteins of Borrelia that plausibly...

Ligand-receptor interactions play a crucial role in the plethora of biological processes. Several methods have been established to reveal ligand-receptor interface, however, the majority of methods are time-consuming, laborious and expensive. Here we present a straightforward and simple pipeline to identify putative receptor-binding sites on the pa...

Interaction of Neisseria meningitidis (NM) with human brain microvascular endothelial cells (hBMECs) initiates of multiple cellular processes, which allow bacterial translocation across the blood-brain barrier (BBB). NM is equipped with several antigens, which interacts with the host cell receptors. Recently we have shown that adhesin MafA (UniProt...

Fish skin is a vital organ that serves a multitude of functions including mechanical protection, homeostasis, osmoregulation and protection against diseases. The expression of skin proteins changes under different physiological conditions. However, little is known about differences in protein expression among various body sites in naïve fish. The o...

One post-doctoral position is available in the laboratory of biomedical microbiology and immunology, Department of microbiology and immunology, University of veterinary medicine and pharmacy in Kosice. The duration of post-doctoral fellowship is 18 months in the first stage. Start of the fellowship is 1.7.2019. If candidate proves good experimental...

Neisseria meningitidis is able to translocate the blood-brain barrier and cause meningitis. Bacterial translocation is a crucial step in the onset of neuroinvasion that involves interactions between pathogen surface proteins and host cells receptors. In this study, we applied a systematic workflow to recover and identify proteins of N. meningitidis...

Borrelia burgdorferi is an extracellular spirochete that causes Lyme disease. Currently, no effective vaccine is available for human and animals except for dogs. In the present study, an extensive bioinformatics pipeline was established to predict new candidates that can serve for vaccine development including building the protein-protein interacti...

Nickel-binding proteins play an important role in the biological processes and can also be utilized in several fields of biotechnology. This study was focused on analysing the nickel-binding proteins from the blood sera of humans ( Homo sapiens ), cattle ( Bos taurus ), sheep ( Ovis aries ), red deer ( Cervus elaphus ), mouflon ( Ovis orientalis ),...

The Central and Eastern European Proteomic Conference (CEEPC) successfully launched its second decade of proteomics in Košice, Slovakia with a program of systems biology, cellular, clinical, veterinary and sports proteomics. Whilst many conferences are struggling to attract participants, CEEPC with its outstanding track record and unique ‘family –...

The mechanisms by which Streptococcus pneumoniae penetrates the blood-brain barrier (BBB), reach the CNS and causes meningitis are not fully understood. Adhesion of bacterial cells on the brain microvascular endothelial cells (BMECs), mediated through protein-protein interactions, is one of the crucial steps in translocation of bacteria across BBB....

Proteins of S. pneumoniae interacting with HBMEC identified with SWATH-MS

Recombinant fragments produced in paper "Proteomic and bioinformatic pipeline to screen the ligands of S. pneumoniae interacting with human brain microvascular endothelial cells"

The penetration of the blood-brain barrier (BBB) and invasion of the central nervous system (CNS) are important steps for all neuroinvasive pathogens. All of the ways of pathogens passing through the BBB are still unclear. Among known pathways, pathogen traversal can occur paracellularly, transcellularly or using a “Trojan horse” mechanism. The fir...

Neisseria meningitidis is a Gram-negative diplococcus responsible for bacterial meningitis and fatal sepsis. Ligand-receptor interactions are one of the main steps in the development of neuroinvasion. Porin B (PorB), neisserial outer membrane protein (ligand), binds to host receptors and triggers many cell signalling cascades allowing the meningoco...

The presence of a blood-brain barrier (BBB) and a blood-cerebrospinal fluid barrier presents animmense challenge for effective delivery of therapeutics to the central nervous system. Many potential drugs, which are effective at their site of action, have failed due to the lack of distribution in sufficient quantity to the central nervous system (CN...

Bacterial meningitis is a devastating worldwide disease. Half of the survivors of meningitis remain with permanent neurological sequelae. The pathogenesis of meningitis is based on a complex host-pathogen interaction. Streptococcus pneumoniae is a life-threatening neuroinvasive pathogen that asymptomatically colonizes the upper respiratory tract. A...

Constant advancements in methodology and mass spectrometry instrumentation, genome sequencing and bioinformatic tools have enabled the identification of numerous pathogen proteomes. Identifying the pathogen interacting proteins by means of high-throughput techniques is key for understanding pathogen invasion and survival mechanisms and in such a wa...

Introduction: Introduction and objective. Lyme disease (LD) is the most common vector-borne disease in the temperate zone of the Northern Hemisphere. Diagnosis of LD is mainly based on clinical symptoms supported with serology (detection of anti- Borrelia antibodies) and is often misdiagnosed in areas of endemicity. Material and methods: In this...

Lyme borreliosis is the most widespread tick-borne zoonosis in the temperate zones of Europe and North America caused by Borrelia burgdorferi. Neuroborreliosis, the most severe manifestation of Lyme borreliosis, is dependent on interactions between borrelia surface proteins and receptors of neurovascular unit cells. The outer surface protein A (Osp...

Proteinuria (measured by urine protein to creatinine ratio, UPCR) is a common finding in canine babesiosis, indicating some degree of renal dysfunction, even when serum levels of creatinine stay within normal range. In order to discover potential novel biomarkers and mechanisms involved in renal dysfunction in canine babesiosis, differences between...

Vector-borne pathogens (VBPs) are a group of globally extended and quickly spreading pathogens that are transmitted by various arthropod vectors. The aim of the present study was to investigate the seroprevalence against Babesia canis, Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, Leishmania infantum, Dirofilaria immitis, and Ehrlichi...

Vector-borne diseases (VBD) are of major importance to human and animal health. In recent years, VBD have been emerging or re-emerging in many geographical areas, alarming new disease threats and economic losses. The precise diagnosis of many of these diseases still remains a major challenge because of the lack of comprehensive data available on ac...

Background Camelids possess unique functional heavy chain antibodies, which can be produced and modified in vitro as a single domain antibody (sdAb or nanobody) with full antigen binding ability. Production of sdAb in conventional manner requires active immunization of Camelidae animal, which is laborious, time consuming, costly and in many cases n...

Central nervous system is protected by the blood-brain barrier, which represents a physical, metabolic and transport barrier and is considered to be a part of a highly dynamic system termed neurovascular unit. Several pathogens, among them viruses, are able to invade the brain. Traversal of viruses across the blood-brain barrier is an essential ste...

Identification of B-cell epitopes is a fundamental step for development of epitope-based vaccines, therapeutic antibodies, and diagnostic tools. Epitope-based antibodies are currently the most promising class of biopharmaceuticals. In the last decade, in-depth in silico analysis and categorization of the experimentally identified epitopes stimulate...

During the last years, miRNA analyses and identifications constitute a new field in molecular biology. miRNA belong to the non- coding RNA family, and are involved in the expression silencing mechanism, by the destruction of the targeted mRNA through interaction with its 3’UTR[1]. miRNA can be exported in blood, and they are more stable than mRNA....

Outer surface protein C of Borrelia stimulates remarkable immune responses during early infection and is currently considered as a leading diagnostic and vaccine candidate. Sensitivity and specificity of serological test based on whole protein OspC is still an issue in the diagnosis of Lyme disease. The minimal B-cell epitopes are key in the develo...

Súhrn T-a B lymfocyty sú sprostredkovatelia adaptívnej imunitnej odpovede a exprimujú antigén-špecifické receptory, kto ré umožňujú rozpoznávať a viazať sa na subdomény cudzích makromolekúl nazývaných epitopy. T lymfocytové epi to py sú časti pohltených a rozštiepených antigénov, ktoré sú prezentované T lymfocytom v asociácii s molekulami hlav né h...

Vector-borne diseases (VBD) presents major threat to the human and animal health, as well as they put substantial burden on livestock production. As a way of sustainable VBD control, focus is set on vaccine development. Advances in genomics and other “omics” over the past two decades have given rise to a “third generation” of vaccines based on tech...

Over the past two decades, library-based display technologies have been staggeringly optimized since their appearence in order to mimic the process of natural molecular evolution. Display technologies are essential for the isolation of specific high-affinity binding molecules (proteins, polypeptides, nucleic acids and others) for diagnostic and the...

Súhrn Lymská borelióza patrí stále k najčastejším kliešťami prenosným ochoreniam v miernom pásme severnej pologule aj napriek zlepšujúcej sa prevencii, diagnostike a terapii. V Európe môžu infekciu spôsobiť viaceré genospecies spirochét patriacich do komplexu Borrelia burgdorferi sensu lato, prenášaných prevažne kliešťami Ixodes ricinus. Riziko nák...

Pathogens pose a major threat for human and animal welfare. Understanding the interspecies host-pathogen protein-protein interactions implicates novel strategies to combat infectious diseases through the rapid development of new therapeutics. The first step in understanding host-pathogen crosstalk is to identify interacting proteins in order to def...

Omics technologies have been developed since decades and used in different thematics. More advancements were done in human and plants thematics. Omics is the conjugation of different techniques, studing all biological molecules (DNA, RNA, proteins, metabolites, etc.). Omics is then able to study entire pathways, elucidating phenotypes and their con...

The aim of the study was to isolate and characterize novel antimicrobial peptides from peptide phage library with antimicrobial activity against multidrug resistant Listeria monocytogenes. Combinatorial phage-display library was used to affinity select peptides binding to the cell surface of multidrug resistant L. monocytogenes. After several round...

West Nile virus (WNV) is a mosquito-borne neurotropic pathogen that presents a major public health concern. Information on WNV prevalence and circulation in Slovakia is insufficient. Oral and cloacal swabs and bird brain samples were tested for flavivirus RNA by RT-PCR using newly designed generic primers. The species designation was confirmed by s...

Francisella tularensis is a Gram-negative bacterium, the causative agent of the zoonotic disease tularaemia. The bacterium has developed several extracellular and intracellular strategies to evade the hosts' innate and adaptive immune responses. The aims of the study were to examine complement sensitivity of wild and attenuated F. tularensis ssp. h...

Protein phosphorylation is a major protein post-translational modification process that plays a pivotal role in numerous cellular processes, such as recognition , signaling or degradation. It can be studied experimentally by various methodologies, including western blot analysis, site-directed mutagenesis, 2D gel elec-trophoresis, mass spectrometry...

Bitter taste has been extensively studied in mammalian species and is associated with sensitivity to toxins and with food choices that avoid dangerous substances in the diet. At the molecular level, bitter compounds are sensed by bitter taste receptor proteins (T2R) present at the surface of taste receptor cells in the gustatory papillae. Our work...

Single Domain Antibody (VHH) is promising building block for various antibody-based applications. Ribosome display can be used successfully in the production of VHH. However, construction of the expression cassette, confirmation of the translation and proper folding of nascent chain and purification of the ribosome complexes still remain cumbersome...

Pathogens have developed sophisticated mechanisms of complement evasion such as binding to the host complement regulatory proteins (CRPs) on their surface or expression of the CRP mimicking molecules. The ability of pathogens to evade the complement system has been correlated with pathogenesis and host selectivity. Hitherto, little work has been un...

Protein phosphorylation is one of the most important protein post-translational modifications and plays a role in numerous cellular processes including recognition, signaling and degradation. It can be studied experimentally by various methodologies, like employing western blot analysis, site-directed mutagenesis, 2 D gel electrophoresis, mass spec...

Protein phosphorylation is one of the most important protein post-translational modifications and plays a role in numerous cellular processes including recognition, signaling and degradation. It can be studied experimentally by various methodologies, like employing western blot analysis, site-directed mutagenesis, 2 D gel electrophoresis, mass spec...

Extracellular form of Francisella is able to cross various cell barriers and invade multiple organs, such as skin, liver, lung and central nervous system. Transient adhesion of Francisella to endothelial cells may trigger the process of translocation. In this report, we showed that Francisella tularensis subsp. holarctica (Fth) is able to adhere to...

Animal production and health (APH) is an important sector in the world economy, representing a large proportion of the budget of all member states in the European Union and in other continents. APH is a highly competitive sector with a strong emphasis on innovation and, albeit with country to country variations, on scientific research. Proteomics (...

Human African Trypanosomiasis (also known as sleeping sickness) is a vector-borne parasitic disease, caused by the genus Trypanosoma. In most cases, disease is transmitted through a bite of an infected tsetse fly (Glossina spp.).Once the parasite enters in the body it successfully evades the immune system. The neuroinvasive form often fails to resp...