Manfred Zinn

Manfred Zinn
HES-SO Valais-Wallis, Sion, Switzerland · Institute of Life Technologies

Prof. Dr.

About

125
Publications
27,834
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3,954
Citations
Citations since 2017
37 Research Items
2009 Citations
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Introduction
Manfred Zinn works at the Institute of Life Technologies, HES-SO Valais-Wallis, where he also leads the research group Biotechnology and Sustainable Chemistry. Manfred's research is focused on bioprocess development using PAT and biosynthesis of biopolyesters (polyhydroxyalkanoates). Manfred is teaching at Bachelor and Master's level at HES-SO Valais-Wallis and at EPFL. One of his most recent publication is "Tailor-made PAT platform for safe syngas fermentations in batch, fed-batch and chemostat mode with Rhodospirillum rubrum". Since January 2018 he is Specialty Chief Editor of the Frontiers' section "Process and Industrial Biotechnology".
Additional affiliations
February 2012 - present
École Polytechnique Fédérale de Lausanne
Position
  • Bachelor and Masters course in Biochemical Engineering
October 2011 - present
University of Applied Sciences and Arts Western Switzerland
Position
  • Bioprocesses and Biomaterials
February 2006 - present
ETH Zurich
Position
  • Genetical Engineering
Description
  • Teaching course in Genetical Engineering

Publications

Publications (125)
Chapter
Among materials emulating fossil plastics in functionality and processability, polyhydroxyalkanoates (PHA) stand out as the sole group that is completely integrated into nature’s closed loop material cycle. Being biobased, biosynthesized, biodegradable, home and industrial compostable, and biocompatible, PHA biopolymers outperform competing polymer...
Article
This study investigated a macro reversible addition fraction chain transfer (RAFT) agent based on three-arm hydroxylated star-shaped poly(3-hydroxy octanoate). The utilization of this star-shaped poly(3-hydroxy octanoate) macro-RAFT agent in the RAFT process resulted in efficient control in the preparation of star-shaped block styrene copolymers. P...
Article
Full-text available
Background Polyhydroxyalkanoates (PHAs) are microbial polyesters synthesized by PHA synthases. Naturally occurring PHA copolymers possess a random monomer sequence. The development of PhaC AR , a unique sequence-regulating PHA synthase, has enabled the spontaneous biosynthesis of PHA block copolymers. PhaC AR synthesizes both a block copolymer poly...
Preprint
Full-text available
Background Polyhydroxyalkanoates (PHAs) are microbial polyesters synthesized by PHA synthase. Naturally occurring PHA copolymers possess a random monomer sequence. The development of PhaC AR , a unique sequence-regulating PHA synthase, has enabled the spontaneous biosynthesis of PHA block copolymers. PhaC AR synthesizes both a block copolymer poly(...
Article
Full-text available
Polyhydroxyalkanoates (PHA) are renewable alternatives to traditional oil-derived polymers. PHA can be produced by different microorganisms in continuous culture under specific media composition, which makes the production process both promising and challenging. In order to achieve large productivities while maintaining high yield and efficiency, t...
Article
Full-text available
Glycolate (GL)-containing polyhydroxyalkanoate (PHA) was synthesized in Escherichia coli expressing the engineered chimeric PHA synthase PhaC AR and coenzyme A transferase. The cells produced poly[GL-co-3-hydroxybutyrate (3HB)] with the supplementation of GL and 3HB, thus demonstrating that PhaC AR is the first known class I PHA synthase that is...
Article
Full-text available
Polyhydroxyalkanoates (PHAs) are a large class of polyesters that are biosynthesized by microorganisms at large molecular weights ( Mw > 80 kDa) and have a great potential for medical applications because of their recognized biocompatibility. Among PHAs, poly(3-hydroxybutyrate), poly(4-hydroxybutyrate), poly(3-hydroxyvalerate), poly(4-hydroxyvalera...
Article
Full-text available
Polyhydroxyalkanoates (PHAs) are a large class of polyesters that are biosynthesized by microorganisms at large molecular weights (Mw > 80 kDa) and have a great potential for medical applications because of their recognized biocompatibility. Among PHAs, poly(3-hydroxybutyrate), poly(4-hydroxybutyrate), poly(3-hydroxyvalerate), poly(4-hydroxyvalerat...
Article
Full-text available
Background Studying bacterial adhesion and early biofilm development is crucial for understanding the physiology of sessile bacteria and forms the basis for the development of novel antimicrobial biomaterials. Microfluidics technologies can be applied in such studies since they permit dynamic real-time analysis and a more precise control of relevan...
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The paper presents a novel instrumental analytical endotoxin quantification assay. It uses common analytical laboratory equipment (HPLC-FLD) and allows quantifying endotoxins (ETs) in different matrices from about 109 EU / mL down to about 40 EU / mL (RSE based). Test results are obtained in concentration units (e.g. ng ET / mL), which can then be...
Preprint
Full-text available
Background Studying bacterial adhesion and early biofilm development is crucial for understanding the physiology of sessile bacteria and forms the basis for the development of novel antimicrobial biomaterials. Microfluidics technologies can be applied in such studies since they permit dynamic real-time analysis and a more precise control of relevan...
Preprint
Full-text available
Background Studying bacterial adhesion and early biofilm development is crucial for understanding the physiology of sessile bacteria and forms the basis for the development of novel antimicrobial biomaterials. Microfluidics technologies can be applied in such studies since they permit dynamic real-time analysis and a more precise control of relevan...
Article
Full-text available
The Swiss Priority Program in Biotechnology of the Swiss National Science Foundation that lasted between 1992 and 2001 had a boosting effect on many biotech disciplines and on the developments of polyhydroxyalkanoates (PHAs) in Switzerland in particular. The funding organization led by Prof. Oreste Ghisalba enabled a better understanding of the PHA...
Article
Full-text available
By the end of 1980s, for the first time polyhydroxyalkanoate (PHA) copolymers with incorporated 4-hydroxybutyrate (4HB) units were produced in the bacterium Cupriavidus necator (formally Ralstonia eutropha) from structurally related carbon sources. After that, production of PHA copolymers composed of 3-hydroxybutyrate (3HB) and 4HB [P(3HB-co-4HB)]...
Article
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Safe-by-Design (SbD) concepts foresee the risk identification and reduction as well as uncertainties regarding human health and environmental safety in early stages of product development. The EU’s NANoREG project and further on the H2020 ProSafe initiative, NanoReg2, and CALIBRATE projects have developed a general SbD approach for nanotechnologies...
Chapter
Polyhydroxyalkanoates (PHAs) are accumulated intracellularly by many bacteria and serve as a carbon and energy storage compound. PHAs are polyesters of high molecular weight and can be isolated by solvent extraction and precipitation in antisolvents. The material properties of PHAs are of great interest due to the inherent biodegradability and exce...
Article
Full-text available
A two-stage chemostat cultivation was used to investigate the biosynthesis of functionalized medium-chain-length polyhydroxyalkanoate (mcl-PHA) in the β-oxidation weakened strain of Pseudomonas putida KTQQ20. Chemostats were linked in sequence and allowed separation of biomass production in the first stage from the PHA synthesis in the second stage...
Article
In the context of the ongoing discussion of accumulating plastic debris in the environment, this article summarizes recent reports on the topic of microplastic pollution. Moreover, it gives an overview of the results from the activities of the research group for Biotechnology and Sustainable Chemistry of the Institute of Life Technologies at the HE...
Article
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Aliphatic polyesters, such as polylactic acid (PLA), polyglycolic acid (PGA), and their copolymer polylactic-co-glycolic acid (PLGA) have become an established choice in the biomedical field in a wide range of applications, from nanoparticles for local drug delivery to bone fixation screws, and, hence, in a huge spectrum of uses in different medica...
Article
Anaerobic production of the biopolymer poly(3-hydroxybutyrate) (PHB) and the monomer 3-hydroxybutyrate (3-HB) was achieved using recombinant clostridial acetogens supplied with syn(thesis) gas as sole carbon and energy source. 3-HB production was successfully accomplished by a new synthetic pathway containing the genes thlA (encoding thiolase A), c...
Article
Full-text available
Syngas from gasified organic waste materials is a promising feedstock for the biotechnological synthesis of the bioplastic poly([R]-3-hydroxybutyrate) (PHB) with Rhodospirillum rubrum. In a first approach, growth studies were carried out with this strain in gas-tight serum vials. When syngas (40% CO, 40% H2, 10% CO2, and 10% N2 v/v) was diluted wit...
Article
Poly(lactate-co-3-hydroxybutyrate) [P(LA-co-3HB)] is produced in engineered Escherichia coli harboring the genes encoding an LA-polymerizing enzyme (LPE) and monomer-supplying enzymes. In this study, high cell-density fed-batch jar fermentation was developed using xylose and/or glucose as the carbon source. Fed-batch fermentation was initially perf...
Article
In 2015 the annual average concentration of atmospheric CO2 rose for the first time above 400 ppm. This raise was clearly related to ever-growing industrial activity and combustion of petrol and its related products, for example, plastics, which annual global production is over 300 Mio tons. An interesting option to approach this problem is the bio...
Article
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Nickel-free stainless steels are of high interest for medical and dental applications, as well as consumer goods where contact with the skin is a consideration. In this paper Prof Efraín Carreño-Morelli and colleagues from the University of Applied Sciences and Arts Western Switzerland evaluate the MIM processing of the high-nitrogen content austen...
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This article provides an overview of activities in the fields of continuous processes, flow chemistry and microreactors at the Universities of Applied Sciences in Switzerland.
Article
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Poly(hydroxyalkanoates) (PHAs) are bacterial polyesters offering a biodegradable alternative to petrochemical plastics. The intracellular formation and degradation of PHAs is a dynamic process that strongly depends on the availability of carbon and other nutrients. Carbon excess and nitrogen limitation are considered to favor PHA accumulation, wher...
Article
Full-text available
Recently, syngas has gained significant interest as renewable and sustainable feedstock, in particular for the biotechnological production of poly([R]-3-hydroxybutyrate) (PHB). PHB is a biodegradable, biocompatible polyester produced by some bacteria growing on the principal component of syngas, CO. However, working with syngas is challenging becau...
Chapter
The production of ever more complex fine chemicals for life sciences (health care, agrochemicals, nutraceuticals) requires selective synthetic biotechnological tools, which complement purely organic chemical synthesis with a rewarding economic and ecological impact. Biotechnology has become an important contributor to the gross domestic product in...
Article
Full-text available
Metal-based electrodes, despite being the most widely used for biomedical applications, are limited by a poor reliable skin-surface interface and patients suffer from comfort issues. The most common problems/inconveniences are caused by stiff electrodes, skin irritation, allergic reaction or corrosion. In order to overcome these problems, we produc...
Chapter
An understanding of how natural materials perform and how their assembly processes work is being applied to guide the design and performance of medical materials at the macro-, micro-, and nanoscale levels. This improved knowledge of natural and hierarchical structures along with advances in polymer science and nanotechnology have become the driver...
Conference Paper
High-nitrogen content austenitic stainless steel parts have been processed by injection moulding of a novel feedstock composed of gas atomized P558 stainless steel powder and biopolymer-based binder. The binder formulation consists of polyhydroxyalkanoate backbone polymer produced by bacterial fermentation, filler wax and surfactant. The solids loa...
Article
Poly(3-hydroxyalkanoates) (PHAs) are bio-based and biodegradable polyesters which have been considered as a promising alternative to petrol-based plastics. Its bacterial production is a dynamic process in which intracellular polymerization and depolymerization are closely linked and depend on the availability of carbon substrates and other nutrient...
Article
Full-text available
Nickel-free stainless steels are used in prosthetics to avoid the harmful effect of nickel-ion release in the human body. Metal powder injection moulding (MIM) allows net-shape processing of complex parts from metal- polymer feedstocks . The feasibility of a novel binder formulation using biosourced polymer produced by bacterial fermentation is exp...
Article
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A profound in vitro evaluation not only of the cytotoxic but also of bioactive potential of a given compound or material is crucial for predicting potential effects in the in vivo situation. However, most of the current methods have weaknesses in either the quantitative or qualitative assessment of cytotoxicity and/or bioactivity of the test compou...
Article
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Certain α/β hydrolases have the ability to hydrolyze synthetic polyesters. While their partial hydrolysis has a potential for surface functionalization, complete hydrolysis allows recycling of valuable building blocks. Although knowledge about biodegradation of these materials is important regarding their fate in the environment, it is currently li...
Article
Polyhydroxyalkanoates (PHAs) are biopolyesters produced by bacteria as intracellular granules under metabolic stress conditions. Many carbon sources such as alkanes, alkenes, alcohols, sugars, fatty acids can be used as feedstock and thus a wide variety of polyesters and monomer units can be potentially synthetized. The work presented here describe...
Article
An overview of activities in the field of Process Analytical Technologies (PAT) at the Universities of Applied Sciences in Switzerland is presented
Article
Bioprocess optimization is a prime target to decrease the cost of functionalized medium-chain-length poly(3-hydroxyalkanoate) (mcl-PHA). We have already demonstrated at the laboratory scale that the pomace of white wine grapes is a promising growth substrate for the biosynthesis of mcl-PHA that is both cheap and does not compete with food and land...
Article
Conjugation of DP18L peptide with (R)-3-hydroxydecanoic acid, derived from the biopolymer polyhydroxyalkanoate, enhances its anti-cancer activity (O Connor et al., 2013). However, it is unknown if other (R)-3-hydroxyalkanoic acids (R3HAs) can enhance peptide activity, if chain length affects enhancement, and what effect R3HAs have on peptide struct...
Article
Full-text available
Background The most successful polyhydroxyalkanoate (PHA) in medical applications is poly(4-hydroxybutyrate) (P4HB), which is due to its biodegradability, biocompatibility and mechanical properties. One of the major obstacles for wider applications of P4HB is the cost of production and purification. It is highly desired to obtain P4HB in large scal...
Article
Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) are biobased and biodegradable alternatives to petrol-derived polymers, whose break-through has been prevented by high production cost. Therefore we investigated whether wastes from the food industry (9 types of fruit pomace including apricots, cherries and grapes, and waste frying oil) could rep...
Chapter
Poly(hydroxyalkanoates), PHAs, are biologically-derived polyesters with fascinating properties. Produced controllably and sustainably during biosynthesis, they can be considered as potential replacements of petroleum-derived plastics in applications ranging from packaging to biomedical materials. The possibilities to chemically functionalize PHAs o...
Article
Full-text available
Poly(4-hydroxybutyrate) (P4HB), belonging to the family of bacterial polyhydroxyalkanoates (PHAs), is a strong, flexible and absorbable material which has a large variety of medical applications like tissue engineering and drug delivery. For efficient production of P4HB recombinant Escherichia coli has been employed. It was previously found that th...
Article
Biodegradable materials, which are currently available for bone tissue regeneration, still have limitations regarding their degradation rate, mechanical stability and/or biological response. Thus, a novel generation of materials for bioactive bone scaffolds is needed that triggers hydroxyapatite formation and can be tailored to suit application-spe...
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Background Elevated pressure, elevated oxygen tension (DOT) and elevated carbon dioxide tension (DCT) are readily encountered at the bottom of large industrial bioreactors and during bioprocesses where pressure is applied for enhancing the oxygen transfer. Yet information about their effect on bacteria and on the gene expression thereof is scarce....
Article
The biodegradable polymer medium chain length polyhydroxyalkanoate (mclPHA), produced by Pseudomonas putida CA-3, was depolymerised and the predominant monomer (R)-3-hydroxydecanoic acid (R10) purified. R10 was conjugated to a d-peptide DP18 and its derivatives. All peptides conjugated with R10 exhibited greater anti-cancer activity compared to the...
Conference Paper
Full-text available
The goal of this work was to develop biodegradable fibers from renewable sources that show best performance with respect to mechanical properties and colonization of tissue engineered cells. Polyhydroxyalkanoates (PHAs) are polyesters that are produced by bacteria, serving as intracellular carbon storage compound. PHAs are advantageous concerning b...
Article
Full-text available
A combination of poly([R]-3-hydroxy-10-undecenoate) (PHUE), a biodegradable polymer from the group of polyhydroxyalkanoates (PHAs), and lipids of different head groups was used to support the growth of calcium phosphate, the main component of mammalian bones. Crystallization took place under two-dimensional films (Langmuir monolayers). The addition...
Article
Poly([R]-3-hydroxy-10-undecenoate) (PHUE) is a biodegradable/biocompatible polyester from polyhydroxyalkanoates (PHAs) family, used in biomedical applications. PHA's introduction to the body may have implications on cell membrane stability—this motivates studies of interactions between PHAs and phospholipids, main membrane constituents. Interaction...
Article
Full-text available
Background Pseudomonas putida KT2440 is able to synthesize large amounts of medium-chain-length polyhydroxyalkanoates (mcl-PHAs). To reduce the substrate cost, which represents nearly 50% of the total PHA production cost, xylose, a hemicellulose derivate, was tested as the growth carbon source in an engineered P. putida KT2440 strain. Results The...
Article
The success of bioprocess implementation relies on the ability to achieve high volumetric productivities and requires working with high-cell-density cultivations. Elevated atmospheric pressure might constitute a promising tool for enhancing the oxygen transfer rate (OTR), the major growth-limiting factor for such cultivations. However, elevated pre...
Article
Much knowledge has been gained for the last 30 years about the effects of pressure on bacteria, and various pressure-based technologies have been designed. The development of modern molecular biology techniques (e.g., DNA microarrays) as well as the technological advances realized in the manufacturing of robust sampling and high-pressure devices ha...
Article
PHBV is produced by bacteria as intracellular carbon storage. It is advantageous concerning biocompatibility and biodegradability, but its low crystallization rate hinders the melt-processing of fibers. This problem can be overcome by combining PHBV with PLA in a core/sheath configuration and introducing a new spin pack concept. The resulting PHBV/...
Article
It has been shown that Pseudomonas putida GPo1 is able to grow in continuous culture simultaneously limited by ammonium (N source) and octanoate (C source), and concomitantly accumulate poly([R]-3-hydroxyalkanoate) (PHA). Under such growth conditions the material properties of PHA can be fine-tuned if a second PHA precursor substrate is supplied. T...
Article
Yeast biofilms contribute to quality impairment of industrial processes and also play an important role in clinical infections. Little is known about biofilm formation and their treatment. The aim of this study was to establish a multi-layer yeast biofilm model using a modified 3.7 l bench-top bioreactor operated in continuous mode (D = 0.12 h(-1))...
Article
Polyhydroxyalkanoates (PHAs) are biodegradable, biocompatible polyesters and very attractive candidates for biomedical applications as materials for tissue engineering. They have a hydrophobic character, but some are able to spread at the air-water interface to form monomolecularly thin films (Langmuir monolayers). This is a very convenient model t...
Article
In this study cutinases from Thermobifida cellulosilytica DSM44535 (Thc_Cut1 and Thc_Cut2) and Thermobifida fusca DSM44342 (Thf42_Cut1) hydrolyzing poly(ethylene terephthalate) (PET) were successfully cloned and expressed in E.coli BL21-Gold(DE3). Their ability to hydrolyze PET was compared with other enzymes hydrolyzing natural polyesters, includi...
Article
Full-text available
The substitution of plastics based on fossil raw material by biodegradable plastics produced from renewable resources is of crucial importance in a context of oil scarcity and overflowing plastic landfills. One of the most promising organisms for the manufacturing of medium-chain-length polyhydroxyalkanoates (mcl-PHA) is Pseudomonas putida KT2440 w...
Article
Medium chain length poly(hydroxyalkanoates) (mcl-PHAs) are bacterial thermoplastic elastomers with a large potential in medical applications. The present study provides a novel process to isolate and purify poly([ R]3-hydroxy-w-undecenoate-co-3-hydroxy-w-nonenoate-co-3-hydroxy-w-heptenoate) (PHUE) and poly([R]-3hydroxy-w-undecenoate-co-3-hydroxy-w-...