Manfred Mielenz

Manfred Mielenz
Research Institute for Farm Animal Biology

Dr.

About

109
Publications
7,007
Reads
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1,502
Citations
Citations since 2016
30 Research Items
842 Citations
2016201720182019202020212022020406080100120
2016201720182019202020212022020406080100120
2016201720182019202020212022020406080100120
2016201720182019202020212022020406080100120
Additional affiliations
April 2002 - December 2012
Rheinische Friedrich-Wilhelms-Universität Bonn

Publications

Publications (109)
Article
Full-text available
In recent years, interest in the larvae of black soldier fly (BSF) ( Hermetia illucens ) as a sustainable protein resource for livestock feed has increased considerably. However, knowledge on the nutritional and physiological aspects of this insect, especially compared to other conventional farmed animals is scarce. This review presents a critical...
Conference Paper
Whole BSFL can be fed to broilers not only for decreasing soybean-protein in diets but also to improve animal welfare. How much whole BSFL could be fed to broilers is however unknown. We assessed apparent interest of broilers in eating BSFL when offered with either 10% (L10), 20% (L20) or 30% (L30) of voluntary feed intake (FI) of control chickens...
Article
Full-text available
Meal of Black Soldier Fly Larvae (BSFL), which requires extraction of protein and fat, is a novel protein source for poultry, while unprocessed whole BSFL could even directly be fed to chickens. Newly hatched Ross-308 chicks (n = 252) received whole BSFL at 10% (L10), 20% (L20) or 30% (L30) of voluntary feed intake (FI) of control chickens (CON) th...
Conference Paper
Soybean meal in poultry diets can be replaced by defatted black soldier fly larvae (BSFL) meal without affecting performance [1]. However, whole BSFL could be fed to chickens directly without processing [2]. We investigated the effects of different inclusion levels of unprocessed BSFL in broiler diets on metabolism, immune status, carcass character...
Conference Paper
Black Soldier Fly larvae (BSFL) are known for their high Ca content [1]. They are one of only a few insect species to contain a wide calcium (Ca):phosphorus (P) ratio of up to 8.2:1 [2] [3]. We hypothesized that inclusion of up to 30% unprocessed BSFL in broiler diets have a positive influence on mineralization, in particular the ash, Ca and P cont...
Conference Paper
The composition of breast milk is influenced by maternal nutrition and plays a pivotal role in offspring development. Among others, milk oligosaccharides (OS) promote the growth of beneficial intestinal bacteria, modulate host-epithelial immune response, and prevent pathogen adhesion. Prebiotics are indigestible dietary fiber to the host, serve as...
Article
Full-text available
Mastitis has a high incidence in dairy cows. Experimental infection with Escherichia coli increased the number of leukocytes in milk and the gene expression of the chemokine receptor CXCR4 in mammary gland tissues. A link between CXCR4 expression and lipopolysaccharide sensing was demonstrated in other species using in vitro models. The receptor th...
Article
Full-text available
Prebiotic supplements and high-protein (HP) diets reduce body weight and modulate intestinal microbiota. Our aim was to elucidate the combined effect of an inulin/oligofructose (FOS) and HP diet on body weight gain, energy metabolism and faecal microbiota. Forty male C57BL/6NCrl mice were fed a control (C) diet for 2 weeks and allocated to a C or H...
Article
Dairy cows are exposed to increased inflammatory processes in the transition period from late pregnancy to early lactation. Essential fatty acids (EFA) and conjugated linoleic acid (CLA) are thought to modulate the inflammatory response in dairy cows. The present study investigated the effects of a combined EFA and CLA infusion on the fatty acid (F...
Conference Paper
Full-text available
Einfluss einer maternalen Supplementierung mit 10% Inulin/FOS zu einer Hochfett-oder Hochproteindiät in Mäusen auf den Trächtigkeitsverlauf und die Entwicklung der Nachkommen F Koch1; M Langhammer2; E Wytrwat2; A Tuchscherer2; HM Hammon1; M Mielenz1; B Kuhla1; CC Metges1 1Institute of Nutritional Physiology "Oskar Kellner", Nutritional imbalances o...
Conference Paper
Full-text available
Impact of a maternal supplementation of 10% inulin/FOS to a high-protein or high-fat diet during pregnancy and lactation in mice on body weight gain, glucose tolerance, mammary gland development and offspring body weight
Article
Full-text available
Diets of dairy cows are often based on maize silage (MS), delivering lower amounts of n-3 fatty acids (FA) compared to grass silage-based diets. The fatty acid composition of the cell membrane can affect the cell function. We evaluated the effects of an MS-based diet on bovine red blood cell (RBC) membrane FA composition and dietary effects on cont...
Conference Paper
Full-text available
Prebiotics such as inulin and oligofructose (FOS) are non-digestible nutritional compounds, which increase gastric filling, intestinal motility and short-chain fatty acid (SCFA) production. FOS supplementation to a high-fat but not control diet prevented body weight gain and exerted positive effects on insulin resistance and lipid metabolism (1). H...
Article
Pigment epithelium-derived factor (PEDF) is evolving as metabolic regulatory protein. Albeit mostly considered in only pathological conditions related to excess energy intake resulting in obesity and insulin resistance, PEDF is likely to be involved in other physiological processes such as the homeorhetic adaptation of metabolism to lactation. We a...
Article
Full-text available
We hypothesized that plasma adipokine concentrations of early-lactation dairy cows are related to body condition score (BCS) at calving and to markers of metabolic status of the cow. As part of a larger study with 117 multiparous Holstein dairy cows, which had high BCS (BCS >4.0) or normal BCS (3.25-3.5) at calving, 22 cows were randomly selected (...
Article
Full-text available
The regulation of growth hormone (GH) release during prenatal development and during early postnatal life is not entirely clarified. In this study plasma GH concentrations in pigs with inherited pseudo vitamin D deficiency type I (PDDR-I), which regularly show growth retardation, were compared during ontogeny with unaffected pigs of the same breed...
Article
Free fatty acid receptors (FFAR) play significant roles in various physiological processes, including energy metabolism, through interaction with their ligands, fatty acids. To determine whether the receptors FFAR1 and FFAR2 are involved in the regulation of liver metabolism during the peripartal period, we selected 13 German Holstein multiparous d...
Article
Full-text available
The pre-weaning period is critical for calf health and growth, and intensive milk feeding programs may assist postnatal development by improving body growth and organ maturation. The aim of the present work was to study the effects of ad libitum milk replacer (MR) feeding on the growth, metabolic adaptation, health, and immune status of newborn cal...
Data
Complete data set of parameters regarding growth and health as shown in Fig 1. (PDF)
Data
Complete data set of data after harvest as shown in Table 3. (PDF)
Data
Cuts indicating the German cutting schema [39]. (PDF)
Data
Complete data set of plasma concentrations of metabolites and hormones as shown in Figs 2 to 5. (PDF)
Data
Complete data set of hepatic mRNA expression. (PDF)
Article
Full-text available
Data on nutrient sensing by free fatty acid receptors (FFAR1, FFAR2, FFAR3, FFAR4) and hydroxycarboxylic acid receptors (HCAR1, HCAR2) are increasing for human or rodent models. Both receptor families link intestinal fermentation by the microbiota and energy metabolism with cellular responses. Therefore, this finding provides a link that is indepen...
Book
Full-text available
Previous studies showed that hcavy milk-fed caIves often develop problems with glucose homeostasis and do not utilise dietary carbohydrates for body fat deposition. This suggests that calves may lack the metabolic capacity for synthesizing fatty acids from glucose. Poor glucose utilisation for growth may aIso be explained by the large supply of die...
Article
The purpose of this study was to evaluate possible effects of quercetin (Q) on liver lipid metabolism and antioxidative status in periparturient dairy cows. The periparturient period is associated with enormous metabolic changes for dairy cows. Energy needs for incipient lactation are too high to be balanced by feed intake, leading to negative ener...
Article
The purpose of this study was to evaluate possible effects of quercetin (Q) on liver lipid metabolism and antioxidative status in periparturient dairy cows. The periparturient period is associated with enormous metabolic changes for dairy cows. Energy needs for incipient lactation are too high to be balanced by feed intake, leading to negative ener...
Article
The transition period in dairy cows is characterized by major changes in glucose and adipose tissue metabolism. The Sirtuin-1 (SIRT1) PPARγ co-activator 1α (PPARGC1A) axis might be related to the adiponectin (ADIPOQ) system to orchestrate the regulation of these processes. We aimed to assess the mRNA abundance of the aforementioned components in on...
Article
The transition from pregnancy to lactation is characterized by major changes in glucose and adipose tissue metabolism. Anti- and prolipolytic pathways mediated via the hydroxycarboxylic acid receptors 1 (HCAR1) and 2 (HCAR2) and tumor necrosis factor-α receptor 1 (TNFR1), as well as the adipokines apelin and resistin, are likely involved in regulat...
Article
Full-text available
We recently showed that the mRNA expression of genes encoding for specific nutrient sensing receptors, namely the free fatty acid receptors (FFAR) 1, 2, 3, and the hydroxycarboxylic acid receptor (HCAR) 2, undergo characteristic changes during the transition from late pregnancy to lactation in certain adipose tissues (AT) of dairy cows. We hypothes...
Article
It is well observed that feeding energy-dense diets in dairy cows during the dry period can cause metabolic imbalances after parturition. Especially dairy cows with high body condition score (BCS) and fed an energy-dense diet were prone to develop production diseases due to metabolic disturbances postpartum. An experiment was conducted to determine...
Article
Full-text available
High ambient temperatures have severe adverse effects on biological functions of high-yielding dairy cows. The metabolic adaption to heat stress was examined in 14 German Holsteins transition cows assigned to two groups, one heat-stressed (HS) and one pair-fed (PF) at the level of HS. After 6 days of thermoneutrality and ad libitum feeding (P1), co...
Article
Insulin-like growth factor-1 (IGF-1), somatolactin and leptin are involved in growth regulation and energy metabolism in fish. We herein focused on serum IGF-1 concentration analysed by enzyme-linked immunosorbent assay in restrictively fed rainbow trout (Oncorhynchus mykiss). The animals were fed a high-fat/low-protein diet at daily feed increases...
Article
Adipose tissue is an endocrine compartment that plays an important role in immune defence by producing and releasing a wide range of proteins, including acute phase proteins (APPs). The liver is the main organ of APP synthesis, although extrahepatic production has also been reported. In the present study, expression of two APPs in dairy cattle, lip...
Article
Full-text available
The transition period in dairy cows (3 weeks prepartum until 3 weeks postpartum) is associated with substantial mobilization of energy stores, which is often associated with metabolic diseases. Nicotinic acid (NA) is an antilipolytic and lipid-lowering compound used to treat dyslipidaemia in humans, and it also reduces non-esterified fatty acids...
Conference Paper
Abstract Text: Apelin, a 77 amino acid preproprotein, which is also known as an adipokine is suggested to play a physiological role in glucose metabolism. It stimulates glucose uptake by adipose tissue in humans and mice, whereas lipolysis in humans is not affected. This might lead to a decreasing lipolysis during the transition period in cattle...
Conference Paper
Abstract Text: Chemerin is thought to be involved in controlling immune responses as a chemoattractant for antigen-presenting cells and has anti-inflammatory as well as pro-inflammatory functions; the latter are linked with obesity and insulin resistance. It is highly expressed as prochemerin in liver and adipose tissue and was recently identified...
Conference Paper
Abstract Text: The mRNA expression of adiponectin (ADIPOQ) and its receptors ADIPOR1/2 in adipose tissue (AT) decreases with the onset of lactation in dairy cows; the serum concentrations of ADIPOQ have also been demonstrated to drop during the lactation-induced negative energy balance (NEB) but not during feed-restriction induced NEB at later st...
Conference Paper
Abstract Text: Chemerin (RARRES2) is involved in adipogenesis and in mediating fat mobilization in mature adipocytes, exerting its effects via its receptor ChemR23. In humans, circulating chemerin levels are regulated by the energy intake. We hypothesized that the expression of RARRES2 or ChemR23 in subcutaneous (SC) and retroperitoneal (RP) adip...
Article
The free fatty acid receptor (FFA) 1, FFA2, FFA3 and hydroxy-carboxylic acid receptor (HCA)2 are G protein-coupled receptors, acting as energy and metabolic sensors. Herein, we characterized the tissue-specific mRNA abundance of genes encoding for these receptors at different stages of lactation. In addition, potential effects of supplementation wi...
Article
Full-text available
Adiponectin and intracellular 5'adenosine monophosphate-activated protein kinase (AMPK) are important modulators of glucose and fat metabolism. Cinnamon exerts beneficial effects by improving insulin sensitivity and blood lipids, e.g., through increasing adiponectin concentrations and AMPK activation. The underlying mechanism is unknown. The Gi/Go-...
Article
Full-text available
In dairy cows the milk associated energy output in early lactation exceeds the input via voluntary feed intake. To spare glucose for mammary lactose synthesis, peripheral insulin sensitivity (IS) is reduced and fat mobilization is stimulated. For these processes a link between IS and the endocrine functions of adipose tissue (AT) is likely; we thus...
Article
Serum amyloid A3 (SAA3) is the predominant SAA isoform secreted by mammary epithelial cells in dairy cows; it is also expressed in bovine adipose tissue (AT). The adipokine SAA3 is linked to obesity and insulin resistance of AT and the respective inflammatory response, at least in mice. Dietary treatment with conjugated linoleic acids (CLA) reporte...
Article
Aquaglyceroporins act as channel proteins and regulate water and glycerol exchange through cell membranes. The aquaglyceroporin aquaporin-7 (AQP7) is abundantly expressed in adipose tissue (AT) and regulates the release of glycerol produced by lipolysis. We aimed to investigate the expression of AQP7 mRNA during lactation in subcutaneous (s.c.) and...
Article
Adiponectin (AdipoQ) is an adipokine mainly secreted by white fatty tissue, playing a major role in energy homeostasis and insulin sensitivity. For cattle, AdipoQ data are largely limited to mRNA expression; to our knowledge, valid information about the AdipoQ protein in bovine tissues and body fluids is not available. Therefore, we have developed...
Article
In addition to its role in energy storage, adipose tissue (AT) is an important endocrine organ and it secretes adipokines. The adipokine adiponectin improves insulin sensitivity by activation of its receptors AdipoR1 and AdipoR2. Lipolysis in AT is downregulated by the G-protein coupled receptor (GPR109A), which binds the endogenous ligand β-hydrox...
Conference Paper
Full-text available
In two trials, Holstein cows were allocated to a conjugated linoleic acid (CLA, Lutrell®, BASF, Germany) or control (Silafat®, BASF) fat supplement at 100 g/day from d 1 to 182 post partum (p.p.) in trial 1 and d 1 to 105 p.p. in trial 2. Trial 1: 21 multiparous cows at d 21 ante partum (a.p.) and d 1, 21, 70, 105, 182, 196, 224, 252 p.p. were biop...
Article
Selection of stable reference genes (REF) is important in real-time PCR data normalization. Bovine tissues such as the mammary gland, liver, muscle, and s.c. fat from the tail head have been thoroughly explored for stable REF, whereas fewer reports exist for other fat depots. Therefore, a suitable combination of REF was tested for different tissues...
Article
The present study aimed to characterize serum haptoglobin (Hp) concentrations throughout an entire lactation period in both primi- and multiparous cows and to compare them to the Hp mRNA expression in liver and - in view of Hp being potentially an adipokine - also in different subcutaneous (s.c.) and visceral fat depots. In addition, potential anti...
Article
The present study aimed to characterize serum haptoglobin (Hp) concentrations throughout an entire lactation period in both primi- and multiparous cows and to compare them to the Hp mRNA expression in liver and - in view of Hp being potentially an adipokine - also in different subcutaneous (s.c.) and visceral fat depots. In addition, potential anti...
Chapter
White adipose tissue (AT) has been considered for a long time as the store house of energy and this tissue is now recognized as an active endocrine organ that communicates with the brain and peripheral tissues by secreting a wide range of hormones and cytokines collectively termed adipokines; the adipokine family includes also several acute phase p...