Manal Hamed

Manal Hamed
National Research Center, Egypt | NRC · Department of Therapeutical Chemistry

Prof. of Biochemistry

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106
Publications
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Introduction
Manal Abdel Aziz Hamed, Professor of Biochemistry, Therapeutic Chemistry Department, National Research Center, Egypt.
Skills and Expertise

Publications

Publications (106)
Article
Context: Hyperglycaemia plays an important role in the development of non-alcoholic fatty liver disease, which is a common complication in diabetics. Objective: The present study aimed to investigate the chemical composition and the efficacy of Commiphora opobalsamum stem bark butanol fraction in ameliorating liver injury associated with diabete...
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Objectives Natural products are often efficacious and safe alternatives to synthetic drugs. This study explored secondary leaves and bark metabolites profiles in extracts of a new Egyptian hybrid, Annona cherimola × Annona squamosa , known as Abdel Razek. This hybrid exhibited 100% similarity with A. cherimola as evidenced by random amplified polym...
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This study focused on investigating the phytochemical composition of the total extract and the ethyl acetate fraction of red grape (Vitis vinifera L.) seeds as well as evaluating the total extract as anti-arthritis agents in rats compering with the new treating agent; interleukin-1 receptor antagonist (IL-1RA) and the classical anti-inflammatory dr...
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Context: Diabetes mellitus (DM) is a metabolic disorder and may lead to cognitive dysfunctions. Objective: The aim of this work is to evaluate the potency of Salvia hispanica L. seeds (S. hispanica L.) (chia seeds) petroleum ether extract in attenuating brain complications associated with streptozotocin (STZ) induced diabetes in rats. Materials...
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Context: Nanotechnology is widely used nowadays in several fields of industry, engineering, and medicine, the biological action mechanisms of AgNPs, which mainly involve the release of silver ions (Ag+), generation of reactive oxygen species (ROS). Objective: The potential toxicity AgNPs of damages to hepatic cells, hesperidin, and naringin role...
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Context: Hepatic encephalopathy (HE) is a severe neuropsychiatric syndrome resulting from liver failure. Objective: To evaluate the protective effect of Schefflera arboricola L. leaves methanol extract against thioacetamide (TAA) induced HE in rats. Materials and methods: GC/MS, LC-ESI-MS, and the total phenolic and flavonoid contents were det...
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Context: Carbon tetrachloride (CCl4) induces oxidative stress in various tissues by altering antioxidants defense system. Recently, there has been a substantial use of phytotherapy to treat different diseases. Objective: This study was designed to evaluate the curative effect of Pulicaria crispa (Forssk.) Benth et Hook (Family Asteraceae) aerial...
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Context: Rheumatoid arthritis (RA) is a chronic, progressive autoimmune disease characterized by aggressive and systematic polyarthritis. Objective: The present study aimed to isolate and identify the phenolic constituents in Brassica oleracea L. (Brassicaceae) seeds methanolic extract and evaluates its effect against rheumatoid arthritis in rats...
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Context Liver is a vital organ for the detoxification of toxic substances in the body, where fibrosis is the major cause of liver damage. Pulicaria crispa processes many therapeutic applications as antioxidant, antimicrobial, anticancer and anti-ulcerative agent. Objective This study aimed to modulate the fibrosis and inflammatory signaling pathwa...
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Context Stomach ulcer is one of the most common gastrointestinal problems in the world. Objective: This study aimed to isolate flavonoid compounds from methanol extract of the aerial parts of Stachytarpheta jamaicensis (L.) Vahl. and evaluate its protective and therapeutic effects against gastric ulcer. Materials and methods: Chromatographic techni...
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From our previous study, the aqueous soluble polysaccharides isolated from cold aqueous extract of Ulva fascinate was prepared and represented as the most of its bioactive constituents. The polysaccharides were formulated as autosomal nanoparticles for use as anti-hepatocirrhosis drugs. The formulations were characterized by their encapsulation eff...
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Potential of Casimiroa edulis and Glycosmis pentaphylla leaves extracts were investigated against the effect of two different particle sizes of silver nanoparticles induced toxicity in mice. Mice received silver nanoparticles (AgNPs) (100 mg/kg) with 20 and 100 nm for four weeks followed by daily oral dose of extracts (500 mg/kg) for three weeks. C...
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Background Parkinson's disease (PD) is one of the most common neurodegenerative disorders spread worldwide in elderly people. Methods The Citrus peel methanolic extract (100 mg/kg body weight) was evaluated as anti-parkinsonism agent in rats through estimation of oxidative stress markers, neurotransmitter levels, energetic indices, DNA fragmentati...
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Background: Hypercholesterolemia is considered as one of the major risk factors for atherosclerosis. The study aimed to evaluate the effect of Ocimum basilieum L. against hypercholesterolemia in rats. Methods: Isolation and identification of flavonoid compounds from the methanolic extract of Ocimum basilicum L. leaves were determined by UV, 1H, 13...
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Recently, severe acute respiratory syndrome coronavirus 2 (SARS-COV-2), commonly known as coronavirus disease-2019 (COVID-19) has rapidly spread across China and around the world. By the declaration of WHO, COVID-19 outbreak considered as a public health problem of international concern. The aim of this study is to provide a comprehensive view on C...
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Introduction: Stomach ulcer is one of the most prevalent disorders worldwide. The study was aimed to isolate and characterize the major polymethoxylated flavonoids in Citrus sinensis peels petroleum ether extract and investigate its protective and curative effect on gastric ulcer. Material and methods: Some spectral analyses were used for identific...
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Parkinson’s disease (PD) is the second common age-related neurodegenerative disease. It is characterized by control loss of voluntary movements control, resting tremor, postural instability, bradykinesia, and rigidity. The aim of the present work is to evaluate curcumin, niacin, dopaminergic and non-dopaminergic drugs in mice model of Parkinson’s d...
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Gastric ulcer affect a large portion of the world population and are induced by several factors, including stress, alcohol consumption, nutritional deficiencies, and ingestion of non-steroidal anti-inflammatory drugs. The objective of this study is to evaluate the protective effect of Annona cherimola as anti-gastro ulcerative agent in rats. The ev...
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Curcuma longa (Family, Zingiberaceae) is rich in polyphenolic compounds; curcuminoids which is used in traditional medicine as antioxidant, anti-tumor, antimicrobial, anti-inflammatory, wound healing, and gastroprotective agents. The aim of the present work is to optimize curcuminoids extraction from C. longa rhizomes using different solvents and e...
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Ficus lyrata leaf extracts in 95% ethanol was fractionated by solvents of increasing polarity and each fraction was screened for its antioxidant activity. The butanol fraction was the most bioactive with 82.40% inhibition of DPPH free radicals. Flavonoids and phenolics of the butanol fraction were identified and quantified by high performance liqui...
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Objective: The aim of the present work is to evaluate the toxicityof titanium dioxide nanoparticles (TiO2NPs) according to their doses and particle sizes. Materials and methods: The effect of 5 days oral administration of TiO2NPs (21 and 80 nm) with different doses (50, 250 and 500 mg/kg body weight) was assessed in mice via measurement of oxida...
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Pulicaria undulata subsp. undulata (Family; Asteraceae) is a medicinal plant used to treat inflammation. The objective of this study is to explore the protective effect of the ethanol extract of P. undulata subsp. undulata aerial parts against ethanol induced gastric ulcer in rats. The chemical composition of plant extract, the unsaponifiable matte...
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Context: Stomach ulcers are the common gastrointestinal disorders worldwide. Objective: This study aimed to investigate the therapeutic impact of Pulicaria crispa aerial part ethanol extract against gastric ulcer in rats. Materials and methods: Ulcer was induced by one oral dose of ethanol (0.5 ml/100g body weight) on 24 hours empty stomach, then t...
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Abstract Background Plantago spp. includes more than 200 species which had been used traditionally to treat many diseases including colds, hepatitis, and infectious diseases. The aim of this study is to evaluate carbohydrates and fixed oil from Plantago ovata L. (Plantaginaceae) seeds against ketoprofen-induced hepatorenal toxicity in rats. Results...
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The antioxidant and hepatoprotective properties of the n-hexane fraction obtained by successive liquid fractionation of the crude cold ethanol extract (95%) of the dried powdered Ficus lutea leaves were evaluated by saponification and GC/MS analysis with column chromatography using silica gel. In vitro antioxidant effect was assessed by DPPH free r...
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The seed oil of Linum grandiflorum was used for centuries as food additive and medicine due to its high content of omega-3. The presented study aimed to characterize the triacylglycerols (TAGs) composition of the seed oil and to study its cytotoxicity and hepatoprotective activity. Based on extensive analysis of the seed oil using ESI-TOF/MS along...
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Background: Chemotherapy of schistosomiasis by certain drugs is impractical due to the resistance of Schistosoma larvae to schistosomiasis drugs after long treatment period. The aim of this work is to monitor Schistosoma mansoni infection on mice livers after treatment with the ethanolic extract of Justicia spicigera areal parts and the herbal anti...
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The aim of the present study was to evaluate different biological activities of Trichoderma viride fungus (Family Hypocreaceae). Trichoderma viride isolated for the first time from the cucumber soil (rhizosphere). It was tested as antimicrobial, antioxidant and anticancer agent. Trichoderma viride from the cucumber soil (rhizosphere) caused inhibit...
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The study aimed to explore the prophylactic effect of melatonin, rowatinex; a naturally occurring renal drug, and its combination on diabetic nephropathy in type 2 diabetic rats. Diabetes was induced by intraperitoneal injection of a single dose of streptozotocin (50 mg/g body weight). Three days before diabetes induction, rats were daily treated w...
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The aim of the present study was to evaluate the hepatoprotective activity of ethyl acetate extract of the liquid culture filtrate of Chaetomium globosum fungus (family Chaetomiaceae). Rats were intraperitoneally injected by CCl4 (0.5ml/kg) twice a week for six consecutive weeks. Treatment tacks (250mg/kg) place at the same time of CCl4 induction a...
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Diabetic encephalopathy is an important complication of diabetes characterized by cognitive impairment, neurochemical and structural abnormalities. This study aimed to investigate the effect of coenzyme Q10 (CoQ10) and niacin as well as their combination in the treatment of encephalopathy associated with streptozotocin (STZ)- induced diabetes in ra...
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This study was designed in order to correlate the flavonoid and lipoidal matters content of Spinach roots and flowers to their hypolipidemic potential. The total flavonoid content was measured via complexation with aluminum chloride while determination of fatty acids methyl esters and unsaponifiable matters in both organs was performed using GC/MS....
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The toxic impact of titanium dioxide nanoparticles (TiO2NPs) on human health is of prime importance owing to their wide uses in many commercial industries. In the present study, the effect of different doses and exposure time durations of TiO2NPs (21nm) inducing oxidative stress, biochemical disturbance, histological alteration and cytogenetic aber...
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Diabetes mellitus (DM) is characterized by hyperglycemia due to insulin inactivity or insufficiency with increasing risk of developing specific complications, including retinopathy, nephropathy, neuropathy, and atherosclerosis. The aim of the present study is to evaluate the efficacy of coenzyme Q10 (CoQ10), niacin, as well as their combination in...
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Objective: To evaluate the anti schistosomiasis activity of the bioactive formed glycoalkaloids from Solanum nigrum L. (family: Solanaceae) (S. nigrum) to control one of the most prevalent parasitic tropical diseases. Methods: Murashige and Skoog media containing growth regulators were used for callus and regeneration establishment. High performanc...
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Objective: To evaluate Brachychiton acerifolius leaf extracts as antidiabetic potential agent and to identify the main active constituents using bioactivity guided fractionation. Methods: In vitro antioxidant activity was evaluated for B. acerifolius different extracts using DPPH assay and vitamin C as control. Antidiabetic activity was then determ...
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Iron deficiency anemia (IDA) is a wide spread syndrome among children especially in the developing countries due to malnutrition, infection or inflammation. The aim of our study was to determine the concentrations of serum trace elements as copper (Cu), zinc (Zn), iron (Fe), magnesium (Mg), and selenium (Se) in IDA Egyptian school children. The wor...
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Diabetic nephropathy is a major complication of diabetes and a leading cause of end-stage renal failure in many developed countries. The study aimed to evaluate the efficiency of certain drugs and melatonin in the treatment of nephropathy secondary to diabetes. Diabetes was induced in rats by a single intraperitoneal injection of streptozotocin (50...
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Terminalia is a genus of Combretaceous plants extensively distributed in tropical and subtropical areas. Numerous species of this genus have been utilized for medicinal purposes because of their valuable phytoconstituents. This study aimed to compare between the phytoconstituents of the hydroalcoholic extract (HAE) of Terminalia bentzone (T. bentzo...
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The novel flavonoids, 2″,2″′-di-O-α-rhamnopyranosyl-vicenin II, a di-C-glycosyl flavone, and herbacetin 3-O-β-xylopyranosyl- (1″′→2″)-O-β-glucopyranoside, were isolated from the leaves of Beta vulgaris subspecies cicla L. var. flavescens, an edible plant which is consumed in the Mediterranean areas, additional to the known flavonoids, 6-C-glucosyl...
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The role of Physalis peruviana (golden berry) as functional food against hepato-renal fibrosis induced by carbon tetrachloride (CCl4) was evaluated. The chemical composition of leaves referred the presence of withanolides and flavonoids. Two compounds, ursolic acid and lupeol, were isolated and their structures were elucidated by different spectral...
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Cold and hot aqueous extraction method (CEM and HEM) of polysaccharides of Dictyopetris membraceae, Padina pavonia, Colpomenia sinusa, Enteromorpha intestinalis, Corallina officinalis, Petrocladia capillraceae and Jania rubes were performed and their physicochemical characterization were studied. The average molecular weight of polysaccharides was...
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Nine phenolics were isolated from the aqueous ethanol extract of the leaves of Caesalpinia ferrea. The isolates were characterized for the first time from that plant. The structures of all isolates (1–9) were elucidated by conventional methods, spectroscopic analysis, including 1D and 2D NMR, and by HR-ESIMS as well. The antioxidant capacities usin...
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The methanolic extract of the leaves of Liquidambar styraciflua L. (Altingiaceae) (LSE) was evaluated for hepatoprotective and antioxidant activities in carbon tetrachloride liver-damaged rats. Hepatotoxicity was induced via intraperitoneal injection of CCl4 1:9 in olive oil, at a dose of 0.5 ml/kg b.wt. The animals received the extract, orally, at...
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Kidney injury molecule-1 (KIM-1), a recently discovered transmembrane protein, is expressed in dedifferentiated proximal renal tubular epithelial cells in damaged regions. Kidney injury early detection in diabetic patients has great importance for therapy and prognosis. Therefore, the aim of the present study is to predict, validate and evaluate th...
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Three sesquiterpene lactones [two germacranolides (micranthin and sintenin) and one guaianolide (4β,10α-dihydroxy-5β,7β,8βH-guaia-1,11(13)dien-12,8α-olide)] and four derivatives of 3-methoxy flavones (santin, quercetagetin-3,6,3'-trimethyl ether, quercetagetin-3,6-dimethyl ether, and 5,7 dihydroxy 3,3',4'-trimethoxy flavone) were isolated from the...
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Objective: The antioxidant and hepatoprotective activities of ethyl acetate (EA) fraction of the dried aerial part of Justicia spicigera were evaluated and the characterization of its anthocyanin content was done. Methods: Hepatic fibrosis was induced by carbon tetrachloride (CCl4) in rats. The ethyl acetate fraction was obtained by successive liqu...
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The objective of this study is to explore and correlate the value of certain biomarkers in breast cancer (BC) females with and without metastasis after undergoing the surgical treatment protocol in the National Cancer Institute in Egypt. Thirty females (33-69 years), diagnosed as early breast cancer patients with or without metastasis, and 20 healt...
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Brachychiton acerifolius, or Sterculia acerifolia as formerly known, is a member of a genus reported for a myriad of bioactive compounds. Metabolome analysis of B. acerifolius - leaves, flowers and seeds - and quantification of its major compounds are demonstrated in this study. Metabolites were analysed via UPLC-PDA-qTOF-( ± ) ESI-MS and UPLC/ITMS...
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The environmentally friendly synthesis of nanoparticles process is a revolutionary step in the field of nanotechnology. In recent years plant mediated biological synthesis of nanoparticles has been gaining importance due to its simplicity and eco-friendliness. In this study, a simple and an efficient eco-friendly approach for the biosynthesis of st...
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Objective: Bromobenzene (BB) is frequently encountered in table-ready foods as contaminant residues. Therefore, the present study is designed to evaluate the petroleum ether extract of Atriplex lindleyi to attenuate the hepato-renal injury induced by BB exposure and study its cytotoxic activity against different human cell line as well as to descri...
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The goals of this study were to develop an in vitro callus growth system of globe artichoke and milk thistle plants and investigate their hypolipidemic and antioxidant activities. For callus induction, leaf explants were cultured on MS medium supplemented with 5 mg/l NAA + 2 mg/l Kin + 0.1 mg/l GA3. For solidifying culture medium, gelrite showed hi...
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The present study aims to evaluate specific biomarkers involved in congenital heart disease (CHD), and whether there is a significant differences between the levels of these biomarkers in the cyanotic CHD (CCHD) and acyanotic CHD (ACHD). We prospectively measured tumor necrosis factor (TNF-α), interleukin-6 (IL-6), C-reactive protein (CRP), vasoend...
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The present ecological study estimated time series trend of relative frequencies (RF) of bilharzial bladder cancer in different Egyptian governorates in relation to climate change during the period 1995-2005. Results revealed that the mean air temperature has positive trend in all the governorates and the number of days of maximum temperature of 45...
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The ethanolic extract of Ficus religiosa and F. microcarpa (Fam. Moraceae) as well as the hexane extract of F. microcarpa and F. mysorensis leaves have been examined for improving the metabolic syndrome secondary to hypercholesterolemia in rats. The evaluation was done through measuring hepatic glucose, glycogen, protein and vitamin C and E levels....
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Due to their worldwide importance for human and animal health, schistosomes are in the focus of national and international research activities. Schistosomiasis is a major health problem and despite decades of research, only one effective drug, Praziquantel is currently available. Recent expansion of sequence databases on Schistosoma mansoni and S....