Manabu Ishitani

Manabu Ishitani
Consultative Group on International Agricultural Research | CGIAR · International Center for Tropical Agriculture (CIAT)

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128
Publications
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Publications

Publications (128)
Article
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Cassava (Manihot esculenta Crantz) is an important crop in Cambodia. The estimation of the yield of cassava stem and tuberous root from plants cultivated at different plant densities in the Battambang province remains elusive, although it is possible to obtain a higher yield these tissues. In this study, we investigated the optimal density of culti...
Article
Full-text available
Key message Status of the current outbreak of cassava mosaic disease (CMD) in Southeast Asia was reviewed. Healthy cassava seed production and dissemination systems have been established in Vietnam and Cambodia, along with integrated disease and pest management systems, to combat the outbreak. Abstract Cassava ( Manihot esculenta Crantz) is one of...
Article
Full-text available
Key message We characterized genes that function in the photoperiodic flowering pathway in cassava. Transcriptome analysis of field-grown plants revealed characteristic expression patterns of these genes, demonstrating that field-grown cassava experiences two distinct developmental transitions. Abstract Cassava is an important crop for both edible...
Article
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Key message: The field survey in this article showed in 'KU50', a popular variety and late-branching type of cassava in Southeast Asia, that flowering rarely occurs in normal-field conditions in Southeast Asia but is strongly induced in the dry season in the mountainous region. Flowering time is correlated with the expression patterns of MeFT1 and...
Article
Full-text available
Background: Identifying new sources of disease resistance and the corresponding underlying resistance mechanisms remains very challenging, particularly in Monocots. Moreover, the modification of most disease resistance pathways made so far is detrimental to tolerance to abiotic stresses such as drought. This is largely due to negative cross-talks b...
Article
Full-text available
In Asia, cassava (Manihot esculenta) is cultivated by more than 8 million farmers, driving the rural economy of many countries. The International Center for Tropical Agriculture (CIAT), in partnership with national agricultural research institutes (NARIs), instigated breeding and agronomic research in Asia, 1983. The breeding program has successful...
Article
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Increasing drought resistance without sacrificing grain yield remains an ongoing challenge in crop improvement. In this study, we report that Oryza sativa CCCH tandem zinc finger protein 5 (OsTZF5) can confer drought resistance and increase grain yield in transgenic rice plants. Expression of OsTZF5 was induced by abscisic acid, dehydration, and co...
Article
Cassava (Manihot esculenta Crantz) is a root crop grown in tropical and subtropical regions. Cassava production faces an increasing number of new diseases and arthropod pests, especially in Southeast Asia, where its area and trade have expanded. An efficient plant-multiplication system is required to provide disease-free plants to farmers to sustai...
Data
Fig. S1. Conventional PCR using cDNA from total RNA. Fig. S2. Conventional PCR using primers for the housekeeping gene G3pdh cDNAs corresponding to total RNA from various tissues. Fig. S3. Electrophoresis run summary using Bioanalyzer 2100 expert_Plant RNA Nano shows RNAs in gels and electropherograms. Fig. S4. Electropherograms of total RNA fro...
Article
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Background The apomictic reproductive mode of Brachiaria (syn. Urochloa) forage species allows breeders to faithfully propagate heterozygous genotypes through seed over multiple generations. In Brachiaria, reproductive mode segregates as single dominant locus, the apospory-specific genomic region (ASGR). The AGSR has been mapped to an area of reduc...
Article
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We developed and modified a precise, rapid, and reproducible protocol isolating high‐quality RNA from tissues of multiple varieties of cassava plants (Manihot esculenta Crantz). The resulting method is suitable for use in mini, midi, and maxi preparations and rapidly achieves high total RNA yields (170–600 μg/g) using low‐cost chemicals and consuma...
Chapter
Cassava is an important crop, in which root tubers have been known as a staple food in tropical area. Recently, cassava tubers are processed by various methods into numerous products and utilized in various ways. The land area of cassava cultivation has been increasing especially in Southeast Asia. Such a situation is causing an introduction of new...
Article
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The burgeoning demand for rice in Latin America and Caribbean (LAC) exceeds supply, resulting in a rice deficit. To overcome this challenge, rice production should be increased, albeit sustainably. However, since rice production is associated with increases in the atmospheric concentration of two greenhouse gases (GHGs), namely methane (CH4) and ni...
Data
Table S1 Agronomic data capture from 2012 well‐watered paddy field trial at CIAT, Palmira, using Ubi:AtGolS2 Curinga lines. Table S2 Agronomic data capture from rainout‐shelter trial (2012‐rainy‐MDSE‐Trial‐1, 2012‐dry‐MDSE‐Trial‐2, and 2014‐rainy‐Trial) at CIAT, Palmira using Ubi:AtGolS2 Curinga transgenic lines. Table S3 Agronomic data capture f...
Data
Figure S1 Expression analysis of drought responsive genes in Ubi:AtGolS2 transgenic rice. Figure S2 Aquapro soil moisture profile and work schedules of Curinga plots during rainout shelter managed drought stress environment (MDSE) trial, CIAT, Palmira. Figure S3 Aquapro soil moisture profile and work schedules of Curinga plots during Target envir...
Article
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Agricultural producers grapple with low farm yields and declining ecosystem services within their landscapes. In several instances, agricultural production systems may be considered largely unsustainable in socioeconomic and ecological (resource conservation and use and impact on nature) terms. Novel technological and management options that can se...
Article
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We evaluated the yields of Oryza sativa L. ‘Nipponbare’ rice lines expressing a gene encoding an A20/AN1 domain stress-associated protein, AlSAP, from the halophyte grass Aeluropus littoralis under the control of different promoters. Three independent field trials were conducted, with drought imposed at the reproductive stage. In all trials, the tw...
Data
Figure S1 Climatic parameters during confined paddy field experiments (August to December, 2012 and February to June, 2013) at CIAT, Palmira. Solar radiation and temperature data are monthly averages and rainfall is monthly total. Figure S2 (a) Rainfall and temperature pattern during crop period in upland confined rainfed field trial at Santa Rosa...
Data
Table S1 Numerical breakdown of consecutive steps in the development of NERICA‐4 NUE lead events. Table S2 Soil physical and chemical properties of confined paddy fields (2012 & 2013) at Palmira and upland rainfed (2014) experiment in Santa Rosa. Table S3 Variation in chlorophyll content in flag leaves among transgenic events and controls grown a...
Article
Accelerated soil-nitrifier activity and rapid nitrification are the cause of declining nitrogen-use efficiency (NUE) and enhanced nitrous oxide (N2O) emissions from farming. Biological nitrification inhibition (BNI) is the ability of certain plant roots to suppress soil-nitrifier activity, through production and release of nitrification inhibitors....
Article
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Drought stress has often caused significant decreases in crop production which could be associated with global warming. Enhancing drought tolerance without a grain yield penalty has been a great challenge in crop improvement. Here, we report the Arabidopsis thaliana galactinol synthase 2 gene (AtGolS2) was able to confer drought tolerance and incre...
Article
Application of field based high-throughput phenotyping (FB-HTP) methods for monitoring plant performance in real field conditions has a high potential to accelerate the breeding process. In this paper, we discuss the use of a simple tower based remote sensing platform using modified single-lens reflex cameras for phenotyping yield traits in rice un...
Article
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Nitrogen (N) fertilizers are a major input cost in rice production and its excess application leads to major environmental pollution. Development of rice varieties with improved nitrogen use efficiency (NUE) is essential for sustainable agriculture. Here, we report the results of field evaluations of marker-free transgenic NERICA4 (New Rice for Afr...
Article
Cassava anthracnose disease (CAD), caused by the fungus Colletotrichum gloeosporioides f. sp. Manihotis, is a serious disease of cassava (Manihot esculenta) worldwide. In this study, we established a cassava oligonucleotide-DNA microarray representing 59,079 probes corresponding to approximately 30,000 genes based on original expressed sequence tag...
Article
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Improved root system architecture can enhance agronomic performance by increasing water and nitrogen (N) acquisition efficiency. However, little is known about interaction between root system architecture and agronomic performance under field environments. To gain a better understanding about the genetic basis of these relationships, we evaluated a...
Article
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Functional analyses of MADS-box transcription factors in plants have unraveled their role in major developmental programs (e.g; flowering and floral organ identity), as well as in stress-related developmental processes such as abscission, fruit ripening and senescence. Over-expression of the OsMADS26 gene in rice (Oryza sativa) has revealed a possi...
Conference Paper
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Soil nitrogen (N) loss due to rapid nitrification (oxidation of ammonium to nitrate) is a serious problem with economic and environmental implications. As a result, a large proportion of N ferti-lisers applied to crops are lost to the environment via nitrate leaching and nitrous oxide emissions. The tropical pasture grass Brachiaria humidicola (Bh)...
Article
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The rice transcription factor WRKY45 plays a central role in the salicylic acid signalling pathway and mediates chemical-induced resistance to multiple pathogens, including Magnaporthe oryzae and Xanthomonas oryzae pv. oryzae. Previously, we reported that rice transformants overexpressing WRKY45 driven by the maize ubiquitin promoter were strongly...
Book
Rice breeding has made important contributions to Latin America. More than 400 cultivars were released from 1975 to 2012, which helped to raise total production to >27 million tonnes obtained from 5.7 million hectares (average for 2010-2012). Rice production provides ∼US$8.8 billion for thousands of farmers in Latin America and the Caribbean (LAC)....
Conference Paper
Full-text available
Nitrification is an oxidation process, part of the larger nitrogen (N) cycle in the soil, and is mediated by microorganisms that transform ammonium (NH + 4) to the water soluble nitrate (NO 3-), producing nitrous oxide (N 2 O, a potent greenhouse gas) as a by-product. Researchers at CIAT-Colombia, in collaboration with JIRCAS-Japan, reported that t...
Article
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Root plasticity, a trait that can respond to different soil environments, may assist plants to scavenge the nutrients in heterogeneous soils. The objective of this paper is to understand the NH4 +-responsive root architectural changes in rice for better acquisition of Nitrogen (N). Using a root basket method, we examined the variation in root plast...
Article
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In rice, seminal root elongation plays an important role in acquisition of nutrients such as N and P, but the extent to which different N forms and P concentrations affect root growth is poorly understood. This study aimed to examine N- and P-mediated seminal root elongation response and to identify putative QTLs associated with seminal root elonga...
Article
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Nitrogen (N), the most critical and essential nutrient for plant growth, largely determines the productivity in both extensive and intensive grassland systems. Nitrification and denitrification processes in the soil are the primary drivers of generating reactive N (NO3-, N2O and NO), largely responsible for N loss and degradation of grasslands. Sup...
Article
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Roots with ideal characteristics are important for sustaining crop yields, particularly when plants are grown in soils with inadequate water and nutrients [1-4]. Understanding the development of roots and their interaction with the soil environment is vital to manipulate the root traits, and ultimately, the food security [5]. For instance, rice has...
Article
Full-text available
Cassava (Manihot esculenta Crantz) is an important crop that provides food security and income generation in many tropical countries, and is known for its adaptability to various environmental conditions. Its draft genome sequence and many expressed sequence tags are now publicly available, allowing the development of cassava polymorphism informati...
Article
Full-text available
The genetic improvement of drought resistance is essential for stable and adequate crop production in drought-prone areas. Here we demonstrate that alteration of root system architecture improves drought avoidance through the cloning and characterization of DEEPER ROOTING 1 (DRO1), a rice quantitative trait locus controlling root growth angle. DRO1...
Article
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Background Agriculture is the single largest geo-engineering initiative that humans have initiated on planet Earth, largely through the introduction of unprecedented amounts of reactive nitrogen (N) into ecosystems. A major portion of this reactive N applied as fertilizer leaks into the environment in massive amounts, with cascading negative effect...
Article
Full-text available
Cassava is an important crop that provides food security and income generation in many tropical countries and is known for its adaptability to various environmental conditions. Despite its global importance, the development of cassava microarray tools has not been well established. Here, we describe the development of a 60-mer oligonucleotide Agile...
Chapter
Cassava is a staple food crop that ensures food security and is a source of income generation for poor farmers in the tropics of many Asian and African countries that are highly prone to harsh and unfavorable environments especially in the context of climate changes. Improving tolerance to abiotic stresses such as drought in this crop is a big chal...