Manabendra Duttachoudhury

• Assam University

Recently, different nanocrystals have been reported to be the alternative, optimistic, and novel antimicrobial agent against the many antibiotic-resistant bacteria. Here, ligand-free CdS and Ag-doped CdS (Ag/CdS) nanocrystals have been synthesized by chemical methods for the study of the antimicrobial activity on Escherichia coli and Staphylococcus...

The liver is not only involved in metabolism and detoxification, but also participate in innate immune function and thus exposed to frequent target Thus, they are the frequent target of physical injury. Interestingly, liver has the unique ability to regenerate and completely recoup from most acute, non iterative situation. However, multiple conditi...

Hepatocellular carcinoma (HCC) is one of the leading causes of death in the modern world and interestingly majority of the cases are related to chronic hepatitis B virus (HBV) infection. HBV-encoded X protein (HBx) is known to play a pivotal role in the pathogenesis of viral infection. HBx is a multifunctional protein of 17 kDa which modulates seve...

Fungal endophytes are epitomes of various secondary metabolites and natural products, which have a place in the medical and industrial arenas. They comprise components of plant microecosystems that dwell asymptomatically and symbiotically within plant tissue systems. Certain contemporaneous endophytes and their specific hosts have established a uni...

Plants and plant products both as extract and derived compounds are known to be used as chemopreventive agents against various types of cancer. Identification of phytochemicals is found to be an effective chemopreventive agent in the field of chemoprevention research. Pharmacological and clinical investigations of medicinal plants have provided a w...

In the era where issue of antibiotic resistance is increasing at an alarming rate and resistance to most antibiotics has occurred, carbapenems have been the choice of drug against most infection because of its unique structure. However due to lack of antibiotic policy carbapenems too have achieved resistance and led to the emergence of carbapenem r...

Biosurfactant is the exudates of microbial metabolism and also familiar as surface-active compounds. It has netted its rank in both industrial and environmental portion but in medical science, use of same is still in nascent stage. Important probiotics like lactic acid bacteria, Bacillus spp. make up the vital constituents of human microflora, poss...

Background: Diabetes mellitus, a chronic disease, has grown wide concern in today’s world. Majority of the world’s population is affected by this disease. All the existing drugs have some side effects such as weight gain, risk of liver disease, skin problems etc. This situation demands the need of more and more potent drugs. Demand for new and fast...

Objective: To evaluate the hepatoprotective activity of aqueous extract of Oxalis debilis Kunth in carbon tetrachloride (CCl4)-induced hepatotoxicity in Swiss albino mice.Methods: Hepatotoxicity was induced by CCl4 30% in olive oil (1 ml/kg intraperitoneally). Mice were treated with aqueous extract of O. debilis at doses of 250 and 500 mg/kg body w...

Objectives: The aim of the present work is to screen the antioxidant potentiality of the bark extract of Oroxylum indicum (L) Vent, ethnomedicinally prescribed plant as hepatoprotective.Methods: Bark extracts were prepared using soxhlet apparatus. Total phenol, flavonoid, and total alkaloid were analyzed using Folin–Ciocalteu assay, aluminum chlori...

Objectives: The aim of the present work is to screen the antioxidant potentiality of the bark extracts of Pajanelia longifolia (Willd.) K. Schuman, ethnomedicinally prescribed plant as hepatoprotective. Methods: Bark extract was prepared using Soxhlet apparatus. Total phenol, flavonoid, total alkaloid were analyzed using Folin–Ciocalteu assay, alum...

The steam distillate of the medicinally important plant Cleome gynandra has been investigated through the Hyphenated technique of Gas Chromatography-Mass Spectroscopy (GC-MS). The distillate incorporated seven major volatile components. Probable structure of one of the major components has been elucidated.

The symptoms of Parkinson's disease (PD) include motor behavioral abnormalities, which appear as a result of the extensive loss of the striatal biogenic amine, dopamine. Various endogenous molecules, including cholesterol, have been put forward as putative contributors in the pathogenesis of PD. Earlier reports have provided a strong link between t...

Introduction: Prenylated and pyrano-flavonoids of the genus Artocarpus J. R. Forster & G. Forster are well known for their acetylcholinesterase (AChE) inhibitory, anti-cholinergic, anti-inflammatory, anti-microbial, anti-oxidant, anti-proliferative and tyrosinase inhibitory activities. Some of these compounds have also been shown to be effective a...

Garo tribe, in most of the remote areas of South Garo Hills district of Meghalaya, India use medicinal plants for their primary needs in all medical aspects due to lack of modern facilities in the district. But this indigenous therapeutic knowledge of the community is fading away rapidly due to lack of proper documentation. The present investigatio...

The study was to evaluate the hepatoprotective activity of aqueous extract of Pyrus pashia Buch.-Ham. ex D. Don against carbon tetrachloride-induced hepatotoxicity in mice. Hepatotoxicity was induced by CCl 4 30% in olive oil (1ml/kg i.p.). Mice were treated with aqueous extract of P. pashia at doses of 250 and 500 mg/kg b.wt. orally for 14 days. T...

Blood being a vehicle for the transport of industrial pollutants in living system, fish hematology is considered as potent biomarker. In the present study, we investigated respective sublethal effects of pulp and paper mill effluents on hematology of two commonly cultured carps, Cyprinus carpio and Ctenopharyngodon idella, using optical, scanning e...

Background: Medicinal plants have always been considered as rich source of antimicrobial agents. Plant derived medicinal preparations are used in simple form of raw plant material or in the refined form of crude extracts against diverse types of diseases. Various medicinal plants which are used as the folk medicine in Bishnupur district, Manipur ar...

Objectives: Nanotechnology-based drug delivery systems can resolve the poor bioavailability issue allied with curcumin. The therapeutic potential of curcumin can be enhanced by making nanocomposite preparation of curcumin with metal oxide nanoparticles, poly lactic-co-glycolic acid (PLGA) nanoparticles and solid lipid nanoparticles that increases...

The therapeutic option of a carbapenem antibiotic is compromised in Pseudomonas aeruginosa owing both to acquired and intrinsic resistance mechanisms. In recent years, New Delhi metallo-β-lactamase has been the focus as a predominant carbapenem resistance determinant. However, it is unclear which of the mechanisms might be adopted by a P. aeruginos...

Aim and objective: Overexpression of efflux pumps belonging to the Resistance Nodulation cell Division (RND) family is the most important intrinsic resistance mechanism of Pseudomonas aeruginosa. Hence, it is imperative to identify suitable efflux pump inhibitors (EPI) that can lead to increased intracellular concentration of antibiotics by blocki...

There is a long tradition of use of medicinal plants for treatment of liver disorders. Documentation and evaluation of hepatoprotective medicinal plants could provide an important role in development of novel drugs for treatment of various hepatic disorders. The present study was aimed to document and perform quantitative evaluation on the medicina...

Hmar tribe, in most of the remote areas of NC hills, Northeast India still use medicinal plants for their primary needs in all medical aspects despite fastest urbanization. This indigenous therapeutic knowledge of the community is fading away rapidly due to lack of proper documentation. Present investigation has been made to document the plants use...

The present study was undertaken to investigate the mutations that are present in mexR gene of multidrug resistant (MDR) isolates of Pseudomonas aeruginosa collected from a tertiary referral hospital of north east India.76 MDR clinical isolates of P. aeruginosa were obtained from the patients who were admitted to or attended the clinics of Silchar...

Since age-old to modern time, plants have assisted as sources of food and medicines for human beings. Natural herbs serve as food supplements and contribute indispensable nutrients in annexation to their use as prophylactic and alleviative potentiality for many diseases. In this context, it can be hypothesized that the eccentric food plants that pe...

Malaria remains one of the major infectious diseases in the tropical region because of the development of resistance by the causal organism in response to the existing drugs. It motivated intensive research to discover drugs having novel structures and mechanisms from the natural sources. Several compounds isolated from natural sources are found to...

Objectives: Cicca acida L., is reported with traditional and pharmacological uses, and a good number of pure compounds have been isolated from its different parts. But published information is sporadic and fragmentary in nature. Therefore, it is imperative to have a comprehensive account of all of its medicinal potentialities with critical analysi...

Pteris semipinnata Linn. (Pteridaceae) (Vernacular name: Skaiumamoidu) is a traditionally used pantropically distributed medicinal pteridophyte of Sothern Assam, India. It grows in tropical forests at altitude below 850m or on rocks; moist, shady base of hillocks and moist road side cuttings. This plant can be harvested throughout the year and spor...

Ethnopharmacological relevance: Being one of the most common types of life threatening diseases in Southern Assam, India, the digestive system disorders (DSD) have gained much attention in recent decades. Traditional beliefs and inadequate income of mass population results in the use of alternative phytotherapies to treat the diseases. Aim of the...

Apart from being used as food, seed oils have also been used traditionally as medicinal products by several communities. However, the full medicinal potential of many seed oil plants is yet to be properly reviewed, particularly for their antimicrobial and antioxidant properties. NorthEast India has rich resources of seed oil plants. The availabilit...

Ricinus communis L. has ethnopharmacological contraceptive reputation but its stem bark has unexplored mechanisms of action in female reproductive system. In the present study, the effect of methanolic and aqueous extracts from the stem bark of the plant was examined on basic porcine ovarian granulosa cell functions and its response to Luteinising...

Carbapenem resistance presents severe threat to the treatment of multidrug resistant Pseudomonas aeruginosa infections. The study was undertaken to investigate the role of efflux pumps in conferring meropenem resistance and effect of single dose exposure of meropenem on transcription level of mexA gene in clinical isolates of P. aeruginosa from a t...

Multidimensional electrospray MS-based strategy mapping approach utilizes two orthogonal MS scanning techniques. These scan methods are combined with liquid chromatography, electrospray mass spectrometry and nanoelectrospray MS/MS is indistinguishable from the conventional MS scan. Anthocyanins are naturally occurring polyphenol compounds that impa...

Information about ethnomedicinal plants used by traditional healers in Tripura, India is very much limited. As there is rapid erosion of traditional knowledge in the state, needs has arisen to document the diversity of medicinal plants, their preparation and associated diseases. Attempts have been made to explore the herbal medicines used by the tr...

Malaria is an infectious disease caused by parasites of the genus Plasmodium. It leads to approximately 1 million deaths per annum worldwide, with an increase number of 6.27 million deaths in 2012 alone. Validation of new antimalarial targets is very important in the context of the rise in resistance to current drugs. One such putative target is th...

Identification of plant use patterns by the traditional communities are significant for determination of related phytochemical and toxicological properties. Information about the taxonomic influence on the medicinal plant selection with relation to disease categories are very limited. The present paper attempted to study the taxonomic influence on...

The advent of carbapenem resistance by the production of β-lectamases and mutated penicillin binding proteins (PBPs) has challenged the treatment of Enterobacteriaceae. Hence there is an urgent need to establish drugs that can fit in the pipeline by overcoming those situations. The hypotheses of the work is based on two facts, i.e., i) design of in...

Abstract Centella asiatica (Gotu Kola) is a plant that grows in tropical swampy regions of the world and has important medicinal and culinary use. It is often considered as part of Ayurvedic medicine, traditional African medicine, and traditional Chinese medicine. The unavailability of genomics resources is significantly impeding its genetic improv...

Plant based compounds serve as curing agents of various disease for decades. Virtual screening using computational tools provide a wide platform of plant based bioactivity prediction. The current study provides molecular mechanism of compound isolated from roots of Piper betle. This work comprises of target prediction and molecular mechanism of Ari...

Pseudomonas aeruginosa is one of the leading opportunistic pathogen and its ability to acquire resistance against series of antimicrobial agents confine treatment option for nosocomial infections. Increasing resistance to fluroquinolone (FQ) agents has further worsened the scenario. The major mechanism of resistance to FQs includes mutation in FQs...

Objectives: Secondary metabolites obtained from different plants have been the starting material for designing different drugs. Polyphenols are the secondary metabolites that shows myriad varieties of pharmacological activities. Different polyphenols have been found to be cardioprotective and paved the path towards development of cardioprotective f...

Secondary metabolites obtained from different plants have been the starting material for designing different drugs. Resveratrol, a naturally occurring polyphenol found in grape skins, peanuts, and red wine shows myriad varieties of pharmacological activities. Different polyphenols have been found to be cardioprotective and paved the path towards de...

Plants have been used in treating cardiovascular diseases (CVD) and are recognized for their ability to produce secondary metabolites. Secondary metabolites obtained from different plants have been the starting material for designing different drugs. Echinosystic acid, a pentacyclic triterpene was isolated and identified from the fruits of Gleditsi...

Natural products have been shown to present interesting biological and pharmacological activities and are used as chemotherapeutic agents. Plants have historically been used in treating cardiovascular diseases (CVD) and are recognized for their ability to produce secondary metabolites. Secondary metabolites obtained from different plants have been...

IntroductionSharing traditional knowledge with the scientific community could refine scientific approaches to phytochemical investigation and conservation of ethnomedicinal plants. As such, integration of traditional knowledge with scientific data using a single platform for sharing is greatly needed. However, ethnomedicinal data are available in h...

Background: Many plants have been listed in the traditional systems of medicine and some of these are providing comprehensive relief to the people suffering from cardio-vascular diseases (CVD) and are recognized for their ability to produce secondary metabolites. Secondary metabolites obtained from different plants have been the starting material f...

Starch is the major carbohydrate reserve in plant storage tissues and can constitute up to 80% of the daily human calorific intake. ADP-glucose pyrophosphorylase (AGPase) is the first rate limiting enzyme of starch biosynthesis pathway and has been exploited as the target for greater starch yield in several plants. The structure-function analysis a...

Supplementary Figure S1. Ramachandran plot of both monocot and dicot AGPase small subunit. (i) Oryza sativa ssp. japonica AGPase small subunit, (ii) Hordeum vulgare AGPase small subunit, (iii) Triticum aestivum AGPase small subunit, (iv) Arabidopsis thaliana AGPase small subunit, (v) Solanum lycopersicum AGPase small subunit, (vi) Beta vulgaris AGP...

Being part of the Indian subcontinent both the North Eastern region of India and the Bangladesh share a long common cultural, economic and political history. One of the most critical problems of developing countries like India as well as Bangladesh is their enormous increase in human population. Contraceptive Prevalence Rate (CPR) of India is 48.3...

The NADPH-dependent HC-toxin reductases (HCTR1 and 2) encoded by enzymatic class of disease resistance homologous genes (Hm1 and Hm2) protect maize by detoxifying a cyclic tetrapeptide, HC-toxin, secreted by the fungus Cochliobolus carbonum race 1(CCR1). Unlike the other classes' resistance (R) genes, HCTR-mediated disease resistance is an inimitab...

Abstract Herbal medicines and traditionally used medicinal plants present an untapped potential for novel molecular target discovery using systems science and OMICS biotechnology driven strategies. Since up to 40% of the world's poor people have no access to government health services, traditional and folk medicines are often the only therapeutics...

Liver is the most important organ playing a major role in the metabolism and excretion in the body. Liver diseases are one of the major threats to public health and are mainly caused by exposure to toxic chemicals, microbes and excessive alcohol consumption. Despite advances in modern medicine, there is need for effective drug that stimulates liver...

Objectives: Flavonoids constitute a large class of polyphenols found in plants. Among flavonoids, Flavonols are the most abundant and widely distributed in nature. Flavonols are present in considerable amounts in fruits and vegetables. They show a wide range of biological activities. In the present study, we wanted to access the cardioprotective ac...

Objectives: Flavonoids constitute a large class of polyphenols found in plants. Among flavonoids, Flavonols are the most abundant and widely distributed in nature. Flavonols are present in considerable amounts in fruits and vegetables. They show a wide range of biological activities. In the present study, we wanted to access the cardioprotective ac...

Objective: The present investigation was aimed to justify the scientific basis in traditional use of Drynaria quercifolia as anti-inflammatory agent in North-East India. Methods: In-vitro antioxidant activities were evaluated by Reducing power method, Superoxide scavenging method and DPPH radical scavenging method. In-vitro and in vivo anti-inflamm...

Traditional remedies used for the treatment of various ailments are considered to be very important in the primary health care of Reang people living in Tripura state of Northeast India. Novel information gathered from the present investigation is important in preserving folk indigenous knowledge of Reang tribe. Systematic and exhaustive field surv...

Centella asiatica is an important medicinal plant with proven medicinal and therapeutic use, but unavailability of genomic resources is significantly impeding its genetic improvement. Until now no attempt has been made to develop Expressed Sequence Tags (ESTs) derived Simple Sequence Repeat (SSR) markers (eSSRs) from Centella genome. Considering th...

More than 2000 plants have been listed in the Traditional (Herbal/Alternative) systems of medicine and some of these are providing comprehensive relief to the people suffering from cardio-vascular diseases, specially “hyperlipidemia” and “ischemic heart disease”. WHO reports indicate that around eighty percent of the global population still relies...

Biodiversity, the variety of life and habitat on earth, plays an important role in ecosystem function. The composition of species in the biodiversity is the result of long lasting evolution. Gradual loss of species on global, national and regional level causes subsequent reduction in ecosystem services. Though species extinction is a natural proces...

Background:The present study was aimed to authenticate scientifically the traditional use of Cyathea gigantea as anti-inflammatory agent in North-East India. Aim:To study antioxidant, anti-inflammatory potentials of the methanolic extract of bark of Cyathea gigantea. Materials and Methods:In this study, extract was evaluated on in-vitroantioxida...

Cervical cancer is the second largest form of cancer to infest the leading cause of death in women worldwide. There are many causes of cancer but viruses are the most common among them. Human papillomaviruses (HPVs) are found to be the causative organism in almost 99.7% of the cases. HPV16 is the most frequent HPV type in malignant neoplastic growt...

The theoretical three-dimensional structure of a novel δ-endotoxin Cry1Id (81 kDa) belonging to Cry1I class, toxic to many of the lepidopteran pests has been investigated through comparative modeling. Molecular dynamics (MD) simulations was carried out to characterize its structural and dynamical features at 10 ns in explicit solvent using the GROM...

Silymarin, a C25 containing flavonoid from the plant Silybum marianum, has been the gold standard drug to treat liver disorders associated with alcohol consumption, acute and chronic viral hepatitis, and toxin-induced hepatic failures since its discovery in 1960. Apart from the hepatoprotective nature, which is mainly due to its antioxidant and tis...

Arundina graminifolia (D. Don)Hochr. Known as bamboo orchid and it is an endangered orchid (Jain and Sastry, 1980; Chowdhery and Murti, 2000). Here a protocol was established for in vitro propagation of bamboo orchid. For micropropagation different concentrations of growth regulators were used and nodal segments were used as explants. MS media supp...

The monocotyledonous Dioscorea genus is known as yam. Many species of Dioscorea genus are economically important crops of worldwide and many of them have been used in the pharmaceutical industry. In vitro propagation of Dioscorea species pave the way to meet the demand of this economically important plant. The protocols are designed to provide the...

Phytochemical screening has been carried out with three different leaf extracts of Croton caudatus Geisel (petroleum ether 60-80, ethyl acetate and methanol), followed by their TLC profiling. Phytochemical analysis reveals the presence of diverse groups of phytochemicals with structural characteristics as evident by their characteristic Rf values i...

Abstract Rice (Oryza sativa L.), a model plant belonging to the family Poaceae, is a staple food for a majority of the people worldwide. Grown in the tropical and subtropical regions of the world, this important cereal crop is under constant and serious threat from both biotic and abiotic stresses. Among the biotic threats, Xanthomonas oryzae pv. o...

Mycobacterium tuberculosis (Mtb), due to its unusual organization crosses different immune barriers and causes tuberculosis. The advent of multidrug resistance tuberculosis (MDR-TB) has attained alarming situation. Hence, computational drug design has been performed in this work to find potent molecules for this purpose. Isoniazid is a widely used...

Pteridophytes, by virtue of possessing great variety and fascinating foliage, have drawn the attention and admiration of horticulturists and plant lovers for centuries. Characterized by an alternation of generations between a well developed sporophyte producing spores and an independent gametophyte producing gametes, the Pteridophyta include over 1...

The pteridophytes include the fern and fern allies which jointly create the necessary environment for human life on earth with other green plants. Dipteris wallichii (R.Br.) T. Moore is a rare and endemic pteridophyte of North East India. It is also facing severe threats due to its habitat destruction. Tribals destroy the plant in large numbers for...

Ethnopharmacological relevance: Vapour-based medicines are an aspect of traditional medicine in North East India. However, no collective studies on this therapy in the region have been attempted. With the changing perception of traditional knowledge, documenting these herbal preparations and the subsequent development of baseline data for applicat...

Tuberculosis is a major disease still today. Though instigation of Directly Observed Therapy, Short Course, (DOTS) has been widely implemented, new infection with tuberculosis in 2010-2011 was still recorded as 8.8 million. The synergy with multidrug resistant strains and HIV epidemics made the tuberculosis treatment difficult and necessitated the...

Cancer is a biomedically complex group of diseases involving cell transformation, dysregulation of apoptosis, proliferation, invasion, angiogenesis and metastasis. The developing knowledge of cancer biology suggests that administering cytotoxic drug therapy at very high doses is not always appropriate.An alternative approach is to administer lower...

In the present work, we made an attempt to assess the antioxidant potential of the rhizome of plant Alocasia decipiens Schott using methanol as solvent. DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical scavenging method and FRAP (ferric reducing antioxidant power) method were used to screen antioxidant activity. Natural antioxidants such as phenol and...

Objective: The highest hypoglycaemic activity of Scoparia dulcis L. in different solvent systems viz. Petroleum ether, Methanol, Ethanol and Acetone was investigated. Materials and Methods: The effects of different extracts of Scoparia dulcis L.was studied to determine the level of efficacy of each extract. The in vivo antidiabetic efficiency was s...