Maksym Vadimovich Ostankov

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine · Department of Cryopathophysiology and Immunology

Investigation of functional state of tumor cells, peculiarities of the pathology development, and sensitivity of tumor cells to the therapeutic agents is an urgent task of modern oncology. It has previously been shown that nanocomplexes (NCs) containing nanoparticles of rare earth orthovanadates GdYEuVO4 and cholesterol are capable of inhibiting th...

Cryopreservation is now considered an integral part of the biotechnological process, exploiting different types of cells and tissues in clinical practice. Among them, dendritic cells (DCs) deserve special attention, notably the immature tolerogenic cells (tolDCs), which provide natural tolerance in humans and animals. High cryolability of tolDCs ha...

Background. Atopic dermatitis (AD) as one of the most common diseases of autoimmune genesis in the structure of dermatological practice, is characterized by itching, dryness, thickening of the skin, characteristic rashes. The drugs of choice in the treatment of AD are steroidal anti-inflammatory drugs. However, the development of unwanted side effe...

One of the urgent problems of modern dermatology is atopic dermatitis (AD), which has multifactorial pathogenesis, the significant prevalence of the disease, the increased frequency of the complicated course, the lack of radical methods of therapy. The expediency to use cryopreserved fetal liver cells (cFLCs) for the treatment of AD is proved by a...

Nanocomplexes (NCs) synthesized at the Institute for Scintillation Materials of the National Academy of Sciences of Ukraine, consisting of nanoparticles (NPs) of gadolinium orthovanadate and cholesterol, are promising compounds that can be used as probes and fluorescent labels for tumor cells or experimental oncopathology treatment. However, the bi...

The review considers the possibility of using modern nanotechnological developments aimed to achieve alternative cryobiological goals. On the one hand, the use of nanomaterials will increase the functional value of thawed cells due to such unique characteristics of nanoparticles as size, shape, surface charge, chemical composition, etc. Nanomateria...

The review presents data on immune pathogenesis of COVID-19 and approaches to its prevention and treatment. The low effectiveness of antiviral drugs is caused by the ability of SARS-CoV-2 virus to change its own structure and functions. Existing treatment strategies of COVID-19 are focused on applying the direct antiviral therapies, modulation of i...

The significance of tumor cells incubation with spherical nanoparticles based on orthovanadates of rare-earth elements activated by europium GdYVO4:Eu3+ in the effectiveness of Ehrlich carcinoma growth inhibition was shown. Ehrlich carcinoma cells were incubated with nanoparticles for 1, 2, 3, and 4 h; afterwards, their accumulation in cells using...

Cryoablation is a method of choice in treatment of solid tumors of different localization. In addition to the destruction of pathological tissue, the cryoablation causes to complex changes in the immune system. This paper briefly discusses the implementation of the abscopic effect after cryosurgery of tumors, as well as possible changes in the immu...

This paper presents the investigations on the impact of lyophilisation conditions on structural and functional characteristics of human cord blood leukoconcentrate (HCBL) such as: the number and viability, cytomorphological indices, content of hematopoietic stem (CD34+) and T-regulatory cells (CD4+CD25high), hematopoietic colony forming units in cu...

The effect of cryopreservation conditions (concentration of dimethyl sulfoxide cryoprotector − DMSO, cooling rate in different temperature ranges) on phenotypic profile and immunomodulatory activity of rat fetal liver cells (FLC) of 14 days of gestation was investigated. The cells were frozen in two modes under the protection of 7.5; 10, and 12.5...

The freezing rate is a decisive factor in determining the purpose of using low temperatures, i.e., for cryoablation or cryobanking of tumor cells. The research aim was to determine effect of different cryopreservation regimens on Ehrlich carcinoma (EC) growth in vivo and subpopulation composition of the formed ascites. The previously cryopreserved...

In the model of adjuvant-induced arthritis in CBA/H mice there were investigated the gematological indices, circulating immune complexes (CIC) in peripheral blood, a number of hematopoietic stem cells: CD34+CD38– and CD117+ in bone marrow (BM) before and after applying either fresh or cryopreserved with various regimens placental cell suspension...

Probl Cryobiol Cryomed 2018; 28(2): 159

Probl Cryobiol Cryomed 2017; 27(2):173.

One of the tasks of current oncology is identification of cancer stem cells and search of therapeutic means capable of their specific inhibition. The paper presents the data on phenotype characteristics of Ehrlich carcinoma cells as convenient and easy-to-follow model of tumor growth. The evidence of cancer stem cells as a part of Ehrlich carcinoma...

Background To study the pathogenesis, diagnosis and therapy of rheumatoid arthritis (RA) there are used numerous experimental in vivo and in vitro models. Topical issue of this problem is to study the causes of disorder and rehabilitation of immune tolerance mechanisms in RA. The concept about the role of different cells in central and peripheral t...

Immune aggression to transplanted allogeneic bone marrow, i.e. the graft-versus-host disease (GVHD), could be decreased by the suppression of effector and/or activation of T- regulatory cells (Treg). This task could be solved by co-transplantaiton of allogeneic bone marrow and mesenchymal stromal cells (MSCs). This study demonstrated the elevated i...

На експериментальних моделях патологій аутоімунного генезу обгрунтована можливість і доведена ефективність застосування продуктів фетоплацентарного комплексу. Продемонстровано їхню здатність до активації Т-регуляторної ланки імунітету і нормалізації клінічних показників експериментальних тварин. Показано більш виражений імунокоригуючий потенціал кр...

Probl Cryobiol Cryomed 2016; 26(2):165.

An actual task of current oncology is the search for the structures, enabling to selectively affect the tumor stem cells, on the expansion rate of those the activity of oncologic process depends. Perspective in this aspect is the use of nanostructures selectively recognizing and inactivating tumor stem cells. In the research there was studied the e...

Tumor development is the consequence of expanding the population of low differentiated cells with unlimited self-maintenance potential, i.e. cancer stem cells (CSCs). Application of new forms of nanocomposites capable of binding to CSCs and inducing the tumor destruction is perspective direction for treating this pathology. There have been develope...

The review highlights some areas of malignant tumor treatment, particularly the cryosurgery of solid tumors. There is discussed the possibility of using the method of cryoablation as a tool for targeting and inactivation of cancer stem cells, being the main structural unit, initiating, maintaining growth and tumor metastasis. The publications regar...

A significant problem of transplantation of histoincompatible bone marrow (BM) is the development of an immune conflict, which is clinically manifested as the graft-versus-host disease (GVHD). This paper concerns the state of hematopoietic system in animals under conditions of experimentally induced GVHD. The pathology was induced by introduction o...

The expediency to use cryopreserved fetal liver cells (cFLCs) for preventive treatment of breast cancer (BC) is proved by a wide range of produced by them biologically active substances with immunomodulatory and antineoplastic activity. Revealing cancer stem cells (CSCs) in the mammary gland (MG) and determination of the immune system (IS) status a...

Rheumatoid arthritis (RA) is one of the most widespread chronic inflammatory diseases of the joints with multifactor etiology. A crucial point in RA is the disorder of proliferation and apoptosis in immunocompetent cells as well as in the cells of monocyte-phagocyte system (MPS). Using the model of adjuvant arthritis (AA) in animals we comparativel...

Aim: Development of tumors results from the expansion of cancer stem cells (CSCs) population. The application of nanocomposites capable of CSCs visualization, binding with them and inducing tumor destruction is a prospective task in oncology. This research aimed to study the CSCs' functional activity as a part of Ehrlich's adenocarcinoma (EAC) afte...

The performed comparative analysis of structural and functional status of fetal liver (FL) hematopoietic stem cells (HSCs) of 14 gestation days and adult bone marrow (BM) of 4-month-old CBA/H mice demonstrated not only the differences of their initial qualitative and quantitative characteristics but also different responses to the effect of cryopre...

N/AÂ Probl Cryobiol Cryomed 2014; 24(2):168.

N/A Probl Cryobiol Cryomed 2014; 24(2):182.

N/A Probl Cryobiol Cryomed 2014; 24(2):185.

N/AÂ Probl Cryobiol Cryomed 2014; 24(2):163.

The study was conducted to assess the effect of gestation term and cryopreservation factors on some functional parameters of mesenchymal stem cells (MSCs) in fetal liver (FL), in particular, ido gene expression rate. Expression rate of ido gene was decreased between 14th to 18th days of FL gestation. Following cryopreservation the expression rate o...

Background An important role in the development of autoimmune reactions during rheumatoid arthritis (RA) is played by CD4+CD25+ T-regulatory cells (Treg) possessing a suppressive activity. The ability of placental structures to produce the activation factors of Treg-cells function – IL-2, TGF-β, to increase the number of cells with Treg phenotype -...

Fetal liver (FL) mesenchymal stem cells (MSCs) are widely applied in cell therapy. One of possible ways of MSCs effect on a recipient's immunocompetent area is the synthesis of tryptophan catabolism enzyme indoleamine 2,3-dioxygenase (IDO). It is known that cryopreservation enables preserving some properties of biological material, as well as chang...

In this research we comparatively analyzed the effect of 3 cryopreservation regimens on structural and functional characteristics of fetal neural cells. The usage of a cryopreservation regimen, designed by the authors of this paper enabled obtaining fetal neural cell suspension with a high viability. This regimen contributed to the integrity of mos...

Nowadays mesenchymal (MSCs) and hematopoetic (HSCs) stem cells of fetal liver (FL) are used in cell and tissue therapy. The important part of application of biological objects in clinical practice is cryopreservation. Stem cells self-renewal and pluripotency are controlled by transcription factors. The most famous are Nanog, Oct4 and SOX2. The anal...

We studied the effect of nanocomposite coatings with various physicochemical properties on the structural and functional properties (adhesion potential, phenotype, gene expression) of mesenchymal stem cells. Of all tested nanocoatings (Al2O3, ZrO2, Ta2O5), oxide coating Al2O3 enriched in vitro monolayer bone marrow cell culture with cells carrying...

The research covers the results of experimental studies of the effect character of nanocomposite coatings with different physical and chemical parameters (type, roughness, hydrophilic-hydrophobic characteristics) on structural and functional properties (adhesive potential, phenotype, gene expression) of mesenchymal stem cells (MSCs). On the tested...

The results of experimental research for the effect character of nanocomposite coatings with different physicochemical parameters on structural and functional properties (adhesive potential, phenotype, expression of ido gene) of multipotent mesenchymal stromal cells (MMSCs) are presented in the work. In the whole spectrum of tested nanocoatings (Al...

Human cord blood (HCB) is a conventional source of stem cells and biologically active substances. Long-term storage of this unique biomaterial is possible under conditions of low temperature bank. In this research we studied the effect of cryopreservation according to two-stage freezing program on cytomorphological and functional characteristics of...

Formation of resistance to influenza virus of type A in mice following introduction of cryopreserved human cord blood leucoconcentrate (cHCBL) or its components (plasma, nucleated cells). Bronchoalveolar lavage was obtained from mucous membrane of respiratory tract in experimental animals during influenza development. Analyzis of cell composition o...

Cancer stem cells (CSC) are the main structural unit in initia tion, support of in situ growth, as well as tumor metastasis during malignant development of various origin. Observed chemo- and radio-resistance of CSC make them a potential target cell to perform cryoeradication. We have performed a comparative study of changes in functional activity...

The structural and functional characteristics of bone marrow (BM) hematopoietic stem cells (HSCs) prior to and after cryopreservation were studied using different methodical approaches. The HSCs colony-forming ability and the cell capability to homing and proliferation were analyzed. We observed a temporary decrease in capability of the cells in de...