Maksym Galkin

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Verified

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• Ph.D.
• PostDoc Position at Danish Cancer Society

Parkinson's disease is a yet incurable, age‐related neurodegenerative disorder characterized by the aggregation of small neuronal protein α‐synuclein into amyloid fibrils. Inhibition of this process is a prospective strategy for developing a disease‐modifying treatment. We overview here small molecule, peptide, and protein inhibitors of α‐synuclein...

Intracellular RNA imaging with organic small molecular probes has been an intense topic, although the number of such reported dyes, particularly dyes with high quantum yields and long wavelength excitation/emission, is quite limited. The present work reports the design and synthesis of three cationic julolidine-azolium conjugates (OX-JLD, BTZ-JLD a...

Parkinson's disease is characterized by the selective death of dopaminergic neurons in the midbrain and accumulation of amyloid fibrils composed of α-synuclein (αSyn). Current treatment involves approaches that compensate the death of dopaminergic neurons by increasing the dopamine levels in remaining cells. However, dopamine can interact with αSyn...

Tissue‐transparent red light is used to release oleic acid, a signaling lipid, into cells, which induces Ca2+ oscillations accompanied by a turn‐on fluorescence response. In their Communication (DOI: 10.1002/anie.202205855), Tomáš Slanina and co‐workers show that π‐extended BODIPY photocages substituted with sulfonate groups can selectively target...

Gewebetransparentes rotes Licht wird verwendet, um Ölsäure, ein signalgebendes Lipid, innerhalb von Zellen freizusetzen, was Ca2+‐Oszillationen auslöst, die mit einer Fluoreszenzantwort einhergehen. In ihrer Zuschrift (DOI: 10.1002/ange.202205855) zeigen Tomáš Slanina und Mitarbeiter, dass π‐expandierte BODIPY‐Photokäfige, die mit Sulfonatgruppen s...

In this work, we developed a water‐soluble caging group based on a π‐extended BODIPY scaffold able to release carboxylate‐containing cargo upon red light illumination (λirr=633 nm). We performed mechanistic studies showing new insights into the principles of the photoreactivity of these cages and demonstrated a significant influence of the structur...

In this work, we developed a water‐soluble caging group based on a π‐extended BODIPY scaffold able to release carboxylate‐containing cargo upon red light illumination ( λ irr = 633 nm). We performed mechanistic studies showing new insights into the principles of the photoreactivity of these cages and demonstrated a significant influence of the stru...

We present fluorogenic cationic organo chalcogens that are highly selective to RNA. We have demonstrated that the conformational dynamics and subsequently the optical properties of these dyes can be controlled to facilitate efficient bioimaging. We report the application of organoselenium and organosulfur‐based cell‐permeable red‐emissive probes be...

Aggregation of small neuronal protein α‐synuclein (αSyn) in amyloid fibrils is considered to be one of the main causes of Parkinson's disease. Inhibition of this aggregation is a promising approach for disease treatment. Dozens of compounds able to inhibit αSyn fibrillization in solution were developed during the last decade. However, the applicabi...

Plaques of amyloid fibrils composed of neuronal protein α-synuclein are one of the hallmarks of Parkinson's disease, and their selective imaging is crucial to study the mechanism of its pathogenesis. However, the existing fluorescent probes for amyloids are efficient only in solution and tissue systems, and they are not selective enough for the vis...

Herein, we introduce versatile molecular tools that enable specific delivery and visualization of photoswitchable lipids at cellular membranes, namely at the plasma membrane and internal membranes. These molecules were prepared by tethering ortho-nitrobenzyl-based fluorescent cages with a signaling lipid bearing an azobenzene photoswitch. They perm...

Researchers prepared the new molecules by tethering ortho-nitrobenzyl-based fluorescent cages with a signaling lipid bearing an azobenzene photoswitch. They permit two sequential photo-controlled reactions: uncaging of a lipid analogue and its repeated activation and deactivation.

Outer membrane vesicles (OMVs), produced by nonpathogenic Gram-negative bacteria, have potentially useful biotechnological applications in extraterrestrial extreme environments. However, their biological effects under the impact of various stressors have to be elucidated for safety reasons. In the spaceflight experiment, model biofilm kombucha micr...

Aggregation of the neuronal protein α-synuclein into amyloid fibrils plays a central role in the development of Parkinson’s disease. Growth of fibrils can be suppressed by blocking fibril ends from their interaction with monomeric protein. In this work we constructed inhibitors that bind to the ends of α-synuclein amyloid fibrils with very high aff...

Parkinson's disease is the second most common neurodegeneration. One of the key processes occurring during its development is the fibrillization of the neuronal protein α-synuclein (αSyn). The team of scientists led by Volodymyr Shvadchak and Dmytro Yushchenko from #IOCBPrague explored the way to block the process by targeting the ends of the growi...

A well-known cationic biocide of guanidine polymer family, polyhexamethylene guanidine hydrochloride (PHMG) has been tested against smooth muscle cells isolated from swine myometrium, synaptosomes of rat brain nerve terminals and rat blood platelets for the membrane action. It was established that PHMG blocked the activity of Na+,K+-ATPase of smoot...

Monodispersed silica-coated magnetite nanoparticles (Fe3O4 @ SiO2) with diameters of 11, 20, and 24 nm were synthesized by thermal decomposition, hydrolysis and condensation of tetramethyl orthosilicate. Substantial Fe3O4 @ SiO2 particle size effects on glutamate transport in brain nerve terminals were observed. The balance between tonic release an...

An excess of the excitatory neurotransmitter, glutamate, in the synaptic cleft during hypoxia/ischemia provokes development of neurotoxicity and originates from the reversal of Na⁺-dependent glutamate transporters located in the plasma membrane of presynaptic brain nerve terminals. Here, we have optimized an electrochemical glutamate biosensor usin...

Carbon dots are a newly discovered class of fluorescent carbon nanosized particles. We revealed recently the neuroactive properties of carbon dots synthesized from β-alanine. We investigate whether or not this effect is inherent to β-alanine-derived carbon dots only? Herein, we found that carbon dots synthesized from different starting materials po...

Background Nanodiamonds are one of the most perspective nano-sized particles with superb physical and chemical properties, which are mainly composed of carbon sp3 structures in the core with sp2 and disorder/defect carbons on the surface. The research team recently demonstrated neuromodulatory properties of carbon nanodots with other than nanodiamo...