Major Singh

Major Singh
Agricultural Scientists Recruitment Board

M Sc Ag, Ph D, FNAAS

About

284
Publications
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Publications

Publications (284)
Article
Full-text available
Outer papery peel of onion bulb is an inevitable bio-waste generated in the course of postharvest handling and processing. Onion peels are rich source of nutraceutically important polyphenolic compounds having many therapeutic potentials. In this study, we characterized onion peel extract (OPE) of eight differentially pigmented short-day onion vari...
Article
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The use of beneficial microbes is hitherto known and constantly increasing in agriculture due to their positive impact on crop growth and yield, and their minimal negative impact on the environment. The objective of this study was to evaluate the impact of eight Trichoderma strains of diverse origin on crop growth and yield of onion under field con...
Article
Full-text available
Haploid induction (HI) holds great promise in expediting the breeding process in onion, a biennial cross-pollinated crop. We used the CENH3-based genome elimination technique in producing a HI line in onion. Here, we downregulated AcCENH3 using the RNAi approach without complementation in five independent lines. Out of five events, only three could...
Article
Full-text available
The current study investigates how genotype by environment interaction (GEI) complicates superior cultivar selection in onion breeding. Employing a randomized block design with three replications, we assessed GEI effects on onion yield and identified adaptable, stable genotypes. The sensitivity of onion genotypes to environmental changes significan...
Article
Full-text available
Radish (Raphanus sativus L.) is an important salad vegetable grown and consumed throughout the world for fleshy roots which has numerous categories– varying in root colour, size, shape and flavour. The uses of coloured radishes in the salads and their anthocyanins as colourants are gaining popularity because of the colour characteristics, health...
Article
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This study used custom-designed sequence-related amplified polymorphism (SRAP) markers to amplify specific regions in the onion genome, resulting in reproducible and informative DNA fingerprints. By analysing various onion accessions, including red, white, and wild types, we identified unique genetic profiles, particularly within Allium roylei and...
Article
Allium cepa is a widely grown crop for its spice and culinary properties. For molecular breeding of Allium cepa, mining and utilization of various sequence-based markers have been widely reported. Unfortunately, none has reported its molecular breeding using potential intron polymorphism (PIP) markers. Due to advantage of intron positioning predict...
Article
Full-text available
The multiplier onion, often known as the potato onion, is a multiplying or aggregating onion that is typically propagated by vegetative means or, in some genotypes, by seeds. The study of genetic divergence and variability in multiplier onions, which is of developing interest, will aid in the careful parent selection for next breeding program with...
Preprint
Full-text available
Haploid induction (HI) holds great promise in expediting the breeding process in onion, a biennial cross-pollinated crop. We used the CENH3-based genome elimination technique in producing a HI line in onion. Here, we downregulated AcCENH3 using the RNAi approach without complementation in five independent lines. Out of five events, only three could...
Article
Wolbachia is a group of bacteria that is known to infect many arthropods and nematodes. It is one of the most common parasitic microbes and is possibly the most common reproductive parasite in the biosphere. The bacterium is best known for its ability to manipulate host reproductive biology where it can induce cytoplasmic incompatibility, parthenog...
Article
Alliums are the most popular for their culinary usage and nutraceutical benefits. Their production is greatly affected by the multiple biotic and abiotic stresses. Poor characterizations of genetic resources are the major bottleneck in the genetic improvement of Alliums. Chloroplast-derived simple sequence repeat has recently gained much popularity...
Article
Full-text available
Introduction Waterlogging is a major stress that severely affects onion cultivation worldwide, and developing stress-tolerant varieties could be a valuable measure for overcoming its adverse effects. Gathering information regarding the molecular mechanisms and gene expression patterns of waterlogging-tolerant and sensitive genotypes is an effective...
Article
Full-text available
Onion thrips, Thrips tabaci Lindeman, an economically important onion pest in India, poses a severe threat to the domestic and export supply of onions. Therefore, it is important to study the distribution of this pest in order to assess the possible crop loss, which it may inflict if not managed in time. In this study, MaxEnt was used to analyze th...
Preprint
Full-text available
Multiplier onion ( Allium cepa var. aggregatum ), commonly known as shallot or potato onion, has been cultivated on a large scale in southern parts of India. It is mostly propagated by bulbs except few genotypes which produces seeds. It is famous for its use in the preparation of sambar which is an important South Indian dish. The multiplier onion...
Chapter
The history of crop genetic manipulation through conventional breeding (artificial selection and selective breeding) dates back to more than 10,000 years. To feed the intense growing population, conventional breeding is unsuitable due to time, money consumption, and lack of desirable traits in plant genetic pool. The introduction of biotechnology i...
Chapter
Alliums are not considered breeder-friendly crops due to their complex nature, breeding behaviour and large genome. The study of trait genetics and breeding of Allium crops is challenging due to some inherent characteristics such as a high degree of cross-pollination, inbreeding depression and biannual life cycle in many alliaceous crops. Hence, de...
Article
Full-text available
Tomato production is severely affected by abiotic stresses (drought, flood, heat, and salt) and causes approximately 70% loss in yield depending on severity and duration of the stress. Drought is the most destructive abiotic stress and tomato is very sensitive to the drought stress, as cultivated tomato lack novel gene(s) for drought stress toleran...
Article
The present study is conducted for the growth and yield improvement of tomato plants under drought stress by inoculation of hexa plant growth-promoting microorganisms (PGPM). Hexa-PGPM consortium (Bacillus megaterium, Pseudomonas fluorescens, P. aeruginosa, P. putida, Paenibacillus polymyxa, and Trichoderma harzianum) is inoculated to the tomato pl...
Article
Full-text available
Onion is the most important crop challenged by a diverse group of insect pests in the agricultural ecosystem. The green semilooper (Chrysodeixis acuta Walker), a widespread tomato and soybean pest, has lately been described as an emergent onion crop pest in India. C. acuta whole mitochondrial genome was sequenced in this work. The circular genome o...
Preprint
Full-text available
Alliums are the most popular for their culinary usage and nutraceutical benefits. Their productions are greatly affected by the multiple biotic and abiotic stresses. Poor characterizations of genetic resources are the major bottleneck in genetic improvement of alliums. Chloroplast derived simple sequence repeat (cpSSR) have recently gained much pop...
Article
Full-text available
The onion (Allium cepa L.) is a lucrative and high-value vegetable–spice crop in India, but it is sensitive to several of diseases caused by fungi, bacteria, viruses, and nematodes, of which a fungal disease, anthracnose, caused by Colletotrichum spp., is a major issue for both onion producers and researchers since it severely affects the bulb prod...
Article
Full-text available
The distinctiveness, uniformity and stability (DUS) testing is important for varietal identification and protection of plant variety. It has become one of the important assays in scientific studies and consequently a major topic of discussion in the International Union for the Protection of New Varieties of Plants (UPOV). Hence, the present study w...
Article
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The garlic is one among the major crop in the genus Allium and mainly consumed as spice. It is asexually propagated through cloves, thus lead to the infestation with numerous viruses and were cause serious threat for its successful cultivation. Availability and assess to the virus free planting material is difficult, hence the experiment was carrie...
Chapter
To overcome the problem of embryo abortion, an in vitro embryo rescue technique was developed to isolate the embryo from developing fruits to avoid abortion. Embryo culture is a useful in vitro breeding technique. It is often used to save embryos from long-distance crossings and embryos that don’t mature normally (as in early ripening and seedless...
Chapter
Vegetatively propagated crops are often challenged with the virus or virus complex in the natural cultivation system. The lack of flowering limits their genetic improvement for virus resistance. Meristem culture can produce virus-free plants; however, the isolation of small size meristem is very difficult and can lead to undesirable somaclonal vari...
Preprint
Allium cepa is a widely grown crop for its spice and culinary properties . For molecular breeding of Allium cepa and its improvement, mining and utilization of various sequence-based markers have been widely reported.Unfortunately, none has reported its molecular breeding using potential intron polymorphism (PIP) markers. Due to advantage of intron...
Article
Onion (Allium cepa L.) is an economically important vegetable that is in high demand around the world. It is a highly cross pollinated crop, which could result in increased diversity and variability. As a result, characterization is a critical component of genetic improvement. Onion genetic resources characterization was done by using the most rece...
Chapter
Full-text available
Onion (Allium cepa L), is an important vegetable crop not only consumed in homes and hotels but also has the highest foreign exchange earner among the fruits and vegetables. Onion is also termed as 'Queen of the kitchen' and it is the most widely cultivated and consumed as a vegetable and medicinal plant in the world. The sorting and grading of the...
Article
Lectins are important for plant defence against various insect and viral invasions. Allium species are rich source of lectins, but till date no lectin is reported to have insecticidal activity against the onion thrips. In the present study, wild Allium species were mined for potential lectins against onion thrips. The full-length lectin genes were...
Preprint
Full-text available
Onion is the most important crop challenged by a diverse group of insect pests in the agricultural ecosystem. The green semilooper (Chrysodeixis acuta Walker), a widespread tomato and soybean pest, has lately been described as an emergent onion crop pest in India. C. acuta whole mitochondrial genome was sequenced in this work. The circular genome o...
Article
Full-text available
Purple blotch (PB) is one of the most destructive foliar diseases of onion and other alliums, caused by a necrotrophic fungal pathogen Alternaria porri. There are no reports on the molecular response of onion to PB infection. To elucidate the response of onion to A. porri infection, we consequently carried out an RNAseq analysis of the resistant (A...
Article
Full-text available
Among Allium crops, onion and garlic are exclusively grown and consumed in the form of fresh as well as processed products. In India, onion and garlic are integral part of daily diet in every household, grown under three crop seasons i.e. kharif, late kharif and rabi. The Rabi crop occupies more than 50% of production. India is the 2nd largest prod...
Article
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Genetic diversity of seventeen garlic genotypes wwas evaluated using morphological characters and molecular markers. Twenty-three morphological characters related to the foliage and bulb traits of garlic were assessed and categorized into quantitative and qualitative parameters. Analysis on the basis of 16 SSR primers was used, out of which 7 produ...
Article
Full-text available
Onion production is severely affected by waterlogging conditions, which are created due to heavy rainfall. Hence, the identification of waterlogging-tolerant onion genotypes is crucial for increasing onion production. In the present study, 100 distinct onion genotypes were screened for waterlogging tolerance under artificial conditions by using the...
Article
Full-text available
Alliums comprise of popular spices and used for various culinary purposes and nutraceuticals. Poor genetic characterization and scarce information regarding domestication, evolution of important alliums like onion, garlic and wild relatives are the major bottleneck to alliums improvement programs. In present study, chloroplast simple sequence repea...
Article
Full-text available
A high-auxin medium, usually used for callus induction, was not effective for Indian short-day onion cv. Bhima super. In this study, we found that the onion seedling radicle was a better explant than shoot tip for embryogenic callus induction, and induction efficiency up to 85.33% along with high embryogenic calli weight was obtained in routinely u...
Article
Garlic chives (Allium tuberosum Rottler ex Sprengel) are an underutilized and neglected food crop of the Allium genus. For the future genetic development of alliums, phenotypic characterization and insights of breeding behaviour could be the aid to breed the new garlic chives varieties. Field Emission Scanning Electron Microscopy (FESEM) was used t...
Cover Page
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ICAR-Directorate of Onion & Garlic Research (DOGR), Pune is a well accomplished institute which focuses on merging innovation with entrepreneurship in Agriculture. Entrepreneurs (Indian citizens) having an innovative business idea in Agri and allied sectors specifically in Onion & Garlic crop are eligible to apply. Detailed information about the pr...
Article
Full-text available
Key message Double transgenic tomato developed by AtDREB1A and BcZAT12 genes pyramiding showed significant drought tolerance by reducing oxidative stress with enhanced yield. AbstractAlthough a large number of efforts have been made by different researchers to develop abiotic stress tolerance tomato for improving yield using single gene, however, n...
Article
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The root-endophytic fungus Piriformospora indica (=Serendipita indica) has been revealed for its growth-promoting effects and its capacity to induce resistance in a broad spectrum of host plants. However, the bioefficacy of this fungus had not yet been tested against any pathogen affecting onion (Allium cepa). In this study, the biocontrol potency...
Article
DNA methylation is an important epigenetic mark and global methylation dynamics regulate plant developmental processes. Even though genome sequencing technologies have made DNA methylation studies easier, it is difficult in non-model species where genome information is not available. Therefore in this study, we developed a simple assay for analysin...
Article
In the present study, three field experiments were conducted to study the effect of sulfur (S) fertilization on yield and biochemical quality of garlic as well as S uptake and thiosulfinate concentration in garlic during the period 2014-17. The experiment consisted of six S treatments (0, 15, 30, 45, 60 and 75 kg S ha−1), and each treatment was rep...
Article
Full-text available
Development of suitable varieties of onion with high yield as well as good shelf life through crossing between long and short-day onion was carried out at the Research Farm of ICAR-Directorate of Onion and Garlic Research, Rajgurunagar, Pune in collaboration with ICAR-CITH, Srinagar. A total of 135 crosses were made between available 15 exotic onio...
Article
Acquisition and germination of seeds are the most desired targets for the improvement of vegetatively propagated crops. In the present study, we developed a potential embryo germination protocol for the Red Globe grape cultivar having a low seed germination rate. Three grape berries at different developmental stages, viz. 50, 60 and 70 days after f...
Chapter
Full-text available
Tomato is one of the most important species of vegetables in the world. Tomatoes can be infected with about 200 diseases which reduce their production. Many resistant genes (R-genes) have been discovered in this plant, whose proteins recognize pathogens’ avirulent proteins and activate the mechanisms for defense. Cf for Cladosporium fulvum, Ve for...
Article
Full-text available
The characterization of garlic germplasm improves its utility, despite the fact that garlic hasn't been used much in the past. Garlic has an untapped genetic pool of immense economic and medicinal value in India. Hence, using heuristic core collection approach, a core set of 46 accessions were selected from 625 Indian garlic accessions based on 13...
Article
Full-text available
Chickpea plant root colonizing bacteria Mesorhizobium ciceri Ca181 promotes plant growth and development through symbiotic association with root nodules. The potentially beneficial effects on plants generated due to this bacterium are mineral nutrient solubilization, abiotic stress tolerance, and nitrogen-fixation, though the molecular mechanisms u...
Article
Full-text available
Consumption of onion seed sprouts is getting popular for its flavour and texture. However, there is limited information on changes in the phytonutrients during sprouting of onion seed. The objective of this study was to determine the temporal variations in the phytonutrient content during the sprouting of onion seeds and to determine the appropriat...
Preprint
Full-text available
A large number of genes has been targeted at the molecular level and transferred in tomato varieties from different sources for drought stress tolerance. Developed single transgenic (ST) plants exhibited better yield under drought stress, although the yield was comparatively lower and the plant growth was reduced. Hence, double transgenic plants we...
Preprint
Full-text available
A high-auxin medium, usually used for callus induction, was not effective for Indian short-day onion cv. Bhima super. In this study, we found that the onion seedling radicle was a better explant than shoot tip for embryogenic callus induction, and induction efficiency up to 85.33% along with high embryogenic calli weight was obtained in routinely u...
Article
Full-text available
Onion (Allium cepa L.) is one of the most important bulbous vegetable used in global kitchen. Although it has various important traits but among them the high total soluble solid content (HTSS) of white onion is highly preferred by the industries for processing purpose. Looking into the broader use of the white onion; we utilized a total of 23 poly...
Article
In salt-prone areas, plant growth and productivity is adversely affected. In the present study, the ZT1-ZT6 transgenic tomato lines having BcZAT12 gene under the regulatory control of the stress inducible Bclea1 promoter were exposed to three salinity levels (50, 100 and 200 mM) at the four leaf stage for 10 days. The transgenic lines showed improv...
Article
Full-text available
Drought is a leading abiotic constraints for onion production globally. Breeding by using unique genetic resources for drought tolerance is a vital mitigation strategy. With a total of 100 onion genotypes were screened for drought tolerance using multivariate analysis. The experiment was conducted in a controlled rainout shelter for 2 years 2017–20...
Article
Drought is the major problem in agricultural production due to loss of moisture content in soil as well as climate variations. Our main aim is to enhance drought tolerance and yield potential in the present study pyramided Arabidopsis thaliana Dehydration Responsive Element Binding1A (AtDREB1A) and Brasica caranata Zinc finger proteins (BcZAT12) tr...
Preprint
Full-text available
Consumptions of onion seed sprouts is getting popular for its flavour and texture. The objective of this study was to determine the temporal variations in the phytonutrient content during the sprouting of onion seeds to determine the appropriate time of harvest. Seeds of three onion varieties (Bhima Red, Bhima Raj and Bhima Kiran) were germinated f...
Preprint
Full-text available
Onion seeds are poor storers they have one of the lowest storability among cultivated crops. Seed moisture is one of the critical factors which determines the seed storability. The effect of ultra-drying of onion seeds on seed storability and seed vigour was studied. Seeds of three onion varieties Bhima Dark Red, Bhima Shakti and Bhima Safed were d...
Article
Full-text available
Onion is an important vegetable crop grown in almost all parts of the World as a flavouring agent in food preparation. It is propagated by seeds and the seed crop is affected by several fungal, viral and bacterial diseases which cause lodging and reduces the seed yield and quality. Onion seed crop was sprayed with different concentration of ethepho...
Article
Full-text available
Garlic is a second most important crop of the genus Allium, known for its traditional medicinal uses. In spite of having medicinal prosperities, it gets infected by several viruses called Garlic Viral Complex (GVC). The garlic viral complex severely affects bulb yield of garlic, due to accumulation of virus load year after year from asexual propagu...
Article
Full-text available
Molecular identification and hybrid purity testing in nineonion hybrids along with their seven parental lines wasconducted at ICAR-Directorate of Onion and GarlicResearch, Rajgurunagar, Pune (Maharashtra). Four out often microsatellite (SSRs- Simple Sequence Repeats) markerswere found to be polymorphic. All four polymorphic markersrevealed polymorp...
Chapter
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Whiteflies (Hemiptera: Sternorrhyncha: Aleyrodidae) are major agricultural pests that cause economic damage worldwide. These pests commonly referred to a group of tiny, soft-bodied, sap-sucking winged insects generally inhabiting on the underside of the leaf. Moreover, they are enormously polyphagous showing intercrop movement, high reproduction, r...
Article
Full-text available
Onion seeds are poor storers they have one of the lowest storability among cultivated crops. Seed moisture is one of the critical factors which determines the seed storability. The effect of ultra-drying of onion seeds on seed storability and seed vigour was studied. Seeds of three onion varieties Bhima Dark Red, Bhima Shakti and Bhima Safed were d...
Article
Full-text available
Onion seeds have one of the lowest longevity among the cultivated crops as it loses viability within a year. The unmarketable seeds have no reported food value or feed value. Onion seeds contain considerable oil content which has potential industrial utility. The present study reports the changes in the quality of seed oil extracted from seeds stor...
Article
Full-text available
Onion seeds have one of the lowest longevity among the cultivated crops as it loses viability within a year. The unmarketable seeds have no reported food value or feed value. Onion seeds contain considerable oil content which has potential industrial utility. The present study reports the changes in the quality of seed oil extracted from seeds stor...
Article
Garlic (Allium sativum L.) is an economically important spice and vegetable crop grown throughout the world. Garlic viral disease complex caused by multiple virus infections is an important constraint in exploiting the potential yield of garlic. Among these viral pathogens, allexivirus (family Alphaflexiviridae) is the genus of viruses known for th...