Maik Damm- Dr.
- Post-Doc at Justus Liebig University Giessen
Maik Damm
- Dr.
- Post-Doc at Justus Liebig University Giessen
Mass Spectrometry about Snake Venoms
About
59
Publications
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360
Citations
Introduction
Current institution
Additional affiliations
December 2019 - February 2020
Position
- Practical Course - Principles of Chemistry
Description
- Supervision
May 2019 - January 2020
March 2019 - present
Position
- Practical Course - Basic Biological Chemistry
Description
- Supervision, Seminar, Protocols
Education
April 2018 - July 2023
April 2015 - December 2017
October 2011 - August 2015
Publications
Publications (59)
Fine-tuned by millions of years of evolution, snake venoms have frightened but also fascinated humanity and nowadays they constitute potential resources for drug development, therapeutics and antivenoms. The continuous progress of mass spectrometry techniques and latest advances in proteomics workflows enabled toxinologists to decipher venoms by mo...
Herein we report on the venom proteome of Vipera anatolica senliki, a recently discovered and hitherto unexplored subspecies of the critically endangered Anatolian meadow viper endemic to the Antalya Province of Turkey. Integrative venomics, including venom gland transcriptomics as well as complementary bottom-up and top-down proteomic analyses, we...
We report a novel hybrid, molecular and elemental mass spectrometry (MS) setup for the absolute quantification of snake venom proteomes shown here for two desert black cobra species within the genus Walterinnesia, Walterinnesia aegyptia and Walterinnesia morgani. The experimental design includes the decomplexation of the venom samples by reverse-ph...
Among venomous animals, toxic secretions have evolved as biochemical weapons associated with various highly specialized delivery systems on many occasions. Despite extensive research, there is still limited knowledge of the functional biology of most animal toxins, including their venom production and storage, as well as the morphological structure...
Venoms have evolved >100 times in all major animal groups, and their components, known as toxins, have been fine-tuned over millions of years into highly effective biochemical weapons. There are many outstanding questions on the evolution of toxin arsenals, such as how venom genes originate, how venom contributes to the fitness of venomous species,...
This syllabus provides a comprehensive framework for a Master’s-level course on Animal Venomics. It includes key topics, references, and resources to support the teaching of venom biology and its applications. Developed through a global collaborative effort.
Blunt-nosed vipers (genus Macrovipera) are among the venomous snakes of highest medical relevance in the Palearctic region. Extensive research has been conducted on their venoms, covering toxin composition, biochemistry, function, pathology and biodiscovery. However, these studies are widely dispersed across the scientific literature, almost exclus...
Snakebite is a neglected tropical disease claiming ~140,000 lives every year. One of the most medically relevant snakes of Asia is the many-banded krait (Bungarus multicinctus). Approximately 8% of the global human population is at risk of being envenomated by this species, able to cause fatal neurotoxicity. Here, we present a proteogenomic and fun...
1 Abstract Snake venom is an ecologically critical functional trait, primarily applied for foraging and accordingly shaped by selective pressures. Recent insights underpinned the high variability of snake venoms down to the intraspecific level, with regional, ontogenetic, and seasonal variation being mostly investigated. In contrast, sex-based veno...
Animal toxins are proteins, peptides or metabolites that cause negative effects against predators, prey or competitors following contact or injection. They work by interacting with enzymes, receptors and other targets causing pain, debilitation or leading even to death. Their biological significance and pharmacological effects in humans make them i...
Planet Earth is threatened by the human population. Energy and resource use are far beyond the planet’s carrying capacity. Planetary Health suggests an alternative idea of prosperity as the best possible human health for all within planetary boundaries. This implies giving priority to ecology because human health depends ultimately on the integrity...
“True” cobras (genus Naja) are among the venomous snakes most frequently involved in snakebite accidents in Africa and Asia. The Cape cobra (Naja nivea) is one of the African cobras of highest medical importance, but much remains to be learned about its venom. Here, we used a shotgun proteomics approach to better understand the qualitative composit...
Non-front-fanged snakes (NFFS) have long been overlooked by snake venom research, likely due to most of them being considered non-medically relevant for humans. The paucity of information about composition and activities of NFFS venoms and oral secretions makes it difficult to assess whether a given species can inflict medically significant bites....
Allopatric populations living under distinct ecological conditions are excellent systems to infer factors underlying intraspecific venom variation. The venom composition of two populations of Vipera ammodytes, insular with a diet based on ectotherms and mainland with a diet based on ectotherms and endotherms, was compared considering the sex and ag...
Snakebite primarily impacts rural communities of Africa, Asia, and Latin America. The sharp-nosed viper (Deinagkistrodon acutus) is among the snakes of highest medical importance in Asia. Despite various studies on its venom using modern venomics techniques, a comprehensive understanding of composition and function of this species' venom remains la...
Spiders are ancient and highly successful predators, which use venom for both predation and defense. Their venoms are complex mixtures of potent biological molecules, emerging as a prolific source of biomolecular innovation in agriculture, biomedicine, and bioeconomy. While small cysteine-rich neurotoxins are typically considered the main component...
Snake venom variations are a crucial factor to understand the consequences of snakebite envenoming worldwide, and therefore it is important to know about toxin composition alterations between taxa. Palearctic vipers of the genera Vipera, Montivipera, Macrovipera, and Daboia have high medical impacts across the Old World. One hotspot for their occur...
Background: Interdisciplinary approaches are particularly important when it comes to complex research areas such as snakebite envenoming. To achieve the World Health Organization's (WHO) goal of halving the number of deaths and disabilities from snakebite by 2030, researchers and experts from different fields need to work together. To promote inter...
Snake venom variations are a crucial factor to understand the consequences of snakebite envenoming worldwide and therefore it's important to know about toxin composition alterations between taxa. Palearctic vipers of the genera Vipera, Montivipera, Macrovipera and Daboia have high medical impacts across the Old World. One hotspot for their occurren...
Introduction: Snakebite is a neglected tropical disease and a globally important driver of death and morbidity. Vipers of the genus Macrovipera (Viperidae: Viperinae) are among the snakes of higher medical importance in the Old World. Despite the medical relevance of Macrovipera venoms, the knowledge regarding them is heterogeneously distributed wi...
The fungal cyclodepsipeptides (CDPs) enniatin, beauvericin, bassianolide, and PF1022 consist of alternating N‐methylated l‐amino and d‐hydroxy acids. They are synthesized by non‐ribosomal peptide synthetases (NRPS). The amino acid and hydroxy acid substrates are activated by adenylation (A) domains. Although various A domains have been characterize...
European vipers (genus Vipera) are medically important snakes displaying considerable venom variation, occurring at different levels in this group. The presence of intraspecific venom variation, however, remains understudied in several Vipera species. Vipera seoanei is a venomous snake endemic to the northern Iberian Peninsula and south-western Fra...
018 – Raine Mercedes "Utilising blood-feeding arthropods to identify coagulotoxins and ion channel modulators" --- 019 – Sam Campbell "Scorpions, Mexico & Antivenom: An electrophysiological investigation of antivenom efficacy"
016 – Yazid Souf "Synthesis and characterization of novel conotoxins from Polynesian cone snails" --- 017 – Mohaddeseh Goudarzi " Structure, function, and evolution of venom toxins produced by nettle caterpillars"
014 – Isabel Fernández "The antiproliferative profile of an octopus venom-peptide" --- 015 – Kity Požek "New insight into the origins of snake venom DC proteins"
012 Collins Awiaga "Unraveling the variation in venom toxin composition for Bitis species" --- 013 – Mátyás Bittenbinder "The good, the bad and the nasty: The in vitro assessment of the effects of cytotoxic snake venoms"
Among venomous animals, toxic secretions have evolved as biochemical weapons associated with various highly specialized delivery systems on many occasions. Despite extensive research, there is still limited knowledge of the functional biology of most animal toxins, including their venom production and storage, as well as the morphological structure...
010 Margareta Lakušić "How (and if) diet shapes the venom composition of the Nose-horned viper?" ---
011 Iris Bea L. Ramiro "Somatostatin analog design by venomous fish-hunting cone snails"
008 Aimee Coulter-Parkhill "Utilising spider venom for diabetes drug use" --- 009 Lorenzo Seneci "Ontogenetic variation in coagulotoxicity in the rattlesnake Crotalus culminatus"
On a first glance, the venom delivery system of snakes seems simple: A fang attached via a duct to a gland. But the contained venoms are highly variable, which makes them to an intensively studied trait by toxinologists. The continuous analytical advances enable them to decipher these mixtures by modern omics technologies, so-called ‘venomics’. Thi...
006 Leah Fitzpatrick "A smorgasbord of selection: Examining evolutionary rates
in mammalian toxins" --- 007 Jonas Krämer "Identification of bioactive compounds from
pseudoscorpion venom"
Known for their venoms, snakes have always fascinated and frightened humanity. Nowadays they constitute important resources for drug and antivenom development and are an intensively studied trait. The continuous analytical advances enable toxinologists to decipher venoms by modern omics technologies, so-called ‘venomics’.
In this contribution, memb...
Snake venom is a complex secretion of proteins, peptides and small compounds. Being closely related to feeding, this important ecological trait is highly variable. The resulting variations are notably characterised by the abundance of different toxins, both at inter-and intraspecific levels. Here we addressed variation in venom composition of two p...
005 Eric Wachtel "From necrosis to haemorrhage: Snake venom cytotoxicity, its underlying mechanisms and the high-throughput methods to study it"
003 Jacob Fröhlich
"Venom analyses of Neuropterans"
---
004 Ekin Varol
"Honey bee venom: stages from collecting to areas of using"
001 Claudia Camarero
"Differential gene expression analyses reveal potential novel predatory toxins in the heteronemertean Lineus longissimus"
---
002 Ignazio Avella
"Venomics of Lataste's viper (Vipera latastei, Bosca 1878): assessment of interpopulational variation and ontogenetic shift"
The comparisons of Western Palaearctic vipers illustrate the appearance of several common toxin families for this region, but the composition ratio can be strongly divers between species as well as based on the used approaches.
The Vipera anatolica senliki is a recently described subspecies of the critically endangered Anatolian Meadow viper (V. an...
Animal venoms are highly complex mixtures of bioactive agents and are investigated for the knowledge of biodiversity and development of potentially new therapeutical strategies. Therefore, neglected and newly discovered species are getting more into the focus of proteomic as well as transcriptomic studies. Over the past years, snake venom analyses...
Herein we report on the venom proteome of Vipera anatolica senliki, a recently discovered and hitherto unexplored subspecies of the critically endangered Anatolian Meadow viper endemic to the Antalya Province of Turkey. Integrative venomics, including venom gland transcriptomics as well as complementary bottom-up and top-down proteomic analyses, we...
Animal secretions are of great interest in terms of drug development due to their complex protein and peptide composition. Especially, in the field of therapeutic medications such as anti-cancer drugs snake venoms receive attention. In this study, we address two Viperidae species from various habitats with a particular focus on the cytotoxic potent...
Animal secretions are of great interest in terms of drug development due to their complex protein and peptide composition. Especially, in the field of therapeutic medications such as anti-cancer drugs snake venoms receive attention. In this study we report of two Viperidae species from various habitats with a particular focus on the cytotoxic poten...
The Asian world is home to a multitude of venomous and dangerous snakes, which are used to induce various medical effects in the preparation of traditional snake tinctures and alcoholics, like the Japanese snake wine, named Habushu. The aim of this work was to perform the first quantitative proteomic analysis of the Protobothrops flavoviridis pit v...
The Asian world is home to a multitude of venomous and dangerous snakes, which are attributed to various medical effects used in the preparation of traditional snake tinctures and alcoholics, like the Japanese snake wine, named Habushu. The aim of this work was to perform the first quantitative proteomic analysis of the Protobothrops flavoviridis p...
In nature, a multitude of organisms rely on the use of biogenic toxins to hunt or to defend themselves, especially snakes shape the image of toxic animals. Over the time, snakes have become excellent hunters around the world, whereby their venom in particular giving them a competitive edge against other predators. The killing of the victim is hereb...
The Nose-horned Viper (Vipera ammodytes) is one of the most widespread and venomous snakes in Europe, which causes high frequent snakebite accidents. The first comprehensive venom characterization of the regional endemic Transcaucasian Nose-horned Viper (Vipera ammodytes transcaucasiana) and the Transdanubian Sand Viper (Vipera ammodytes montandoni...
Vipers are one of the largest venomous snake families, that can be found in a wide range all over the world. A large agglomeration of various vipers is found around the Mediterranean Sea, where the Transdanubian Sand Viper (Vipera ammodytes montandoni), with their main area of occurrence in Bulgaria, was captured in the north-west of Turkey. The Tr...
Questions
Questions (2)
Dear all,
are there any big differences for the long term storage (weeks upto month/years) of proteins/tryptic digest peptides, which where dried by Speedvac (stored at roomtemperature or +4°C?) and ZipTips, for a following ESI-MS?
I would be really greatfull for any suggestions or literature.
Dear all,
I'm trying to investigate the hydrolytic cleavage/ degradation of a small protein by mass spectrometry (LC-ESI-Orbitrap).
I can see a time dependent formation of smaller peptides and could already annotate some based on the amino acid sequence of the protein, however annotating all manually is very time consuming. I am therefore looking for a some software that would be able to annotate the protein fragments based on my input sequence (considering charge states, Na/K adducts and internal fragments).
I imagine something similiar to software dealing with MS/MS spectra however dealing with MS1 Scans and fragments resulting from hydrolysis (C-term: COOH) rather than b/y type fragmentation (C-term: -18Da). I would be really greatfull for any suggestions. :)
