Maidul Hossain

• PhD
• Professor (Assistant) at Vidyasagar University

A sustainable, efficient, and eco-friendly approach was developed to synthesize silver nanoparticles using Euphorbia neriifolia leaf extract. The biosynthesized Euphorbia neriifolia silver nanoparticles (ENSNPs) were comprehensively characterized using several techniques, including absorbance spectroscopy, Fourier transform infrared spectroscopy, h...

Small molecules as ligands target multifunctional ribonucleic acids (RNA) for therapeutic engagement. This study explores how the anticancer DNA intercalator harmine interacts various motifs of RNAs, including the single-stranded A-form poly (rA), the clover leaf tRNAphe, and the double-stranded A-form poly (rC)-poly (rG). Harmine showed the affini...

The interaction of deoxyribonucleic acid (DNA) with medicinally significant small molecules has long piqued the interest of researchers because its applications are directly related to the discovery of new classes of drugs. Keeping this in mind, here we report berberine derivatives and their interaction with calf thymus DNA (CT-DNA). In this report...

The main effectors in the innate immune system of Bombyx mori L. are antimicrobial peptides (AMPs). Here, we infected B. mori with varied inoculum sizes of Pseudomonas aeruginosa ATCC 25668 cells to investigate changes in morpho-anatomical responses, physiological processes and AMP production. Ultraviolet-visible spectra revealed a sharp change in...

A multistep approach was undertaken for biobutanol production targeting valorization of agricultural waste. Optimum production of lignocellulolytic enzymes [CMCase (3822.93U/mg), FPase (3640.93U/mg), β-glucosidase (3873.92U/mg), xylanase (3460.24U/mg), pectinase (3359.57U/mg), α-amylase (4136.54U/mg), and laccase (3863.16U/mg)] was accomplished thr...

Khambir is a leavened staple food among the native highlanders of Western Himalaya. It is prepared by sourdough fermentation of wheat flour with yeast (YAK) or buttermilk (BAK). Both types of bread were rich in carbohydrate, protein, dietary fiber, containing less fat and gluten, and enriched with lactic acid, vitamins, and minerals. The in vitro d...

Quantitative determination of hemoglobin (Hb) level is an obligatory part of diagnostic proceedings and an undisputed health index for a number of chronic diseases. In this study, we have tried to introduce a new 9-N-substituted berberine analogue (BR-N) as a triumphant fluorescent sensor for aptly selective and extremely sensitive nanomolar detect...

Here, Tagetes erecta leaves extract mediated silver nanopartices (te-SNPs) were synthesized. This synthesis process was simple, one step and eco-friendly. The te-SNPs were characterized by various spectroscopic instruments such as ultraviolet visible (UV–vis), Fourier-transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS) and Transm...

In the present investigation, the characterization of Cassia fistula leaf extracts (CFLE) mediated gold nanoparticles (CF-GNPs) and its binding features with human serum albumin (HSA) through interaction have been probed. The results from UV-visible, TEM and EDX analysis proved the formation of CF-GNPs. The functional groups like OH, NH, CN etc pre...

Optical signaling of serum albumin (SA) provides precious information to realize their natal functions, in addition to develop biomedical applications. Herein, we report a new class of easy synthesizable and water-soluble compounds (BZ1-BZ5) based on different positioning chlorine in 9-O-benzyl substituted berberine scaffolds for selective detectio...

Exploit of biomass as an inexhaustible resource has accepted much more curiosity to the present research world. Herein, a simple, one-step solvothermal action has been used to synthesize an ascendable amount of fluorescent carbon dots (CDs) with an average size of~3.13 nm, from Low-reasonable and green source lychee waste. The excitation/emission m...

Current research on the detection of serum albumin (SA) by fluorescence-based sensing probe has earned massive interest due to its various proficiencies. In this belief, we report a cost-effective fluorescence probe BRDR4 from well-known medicinal herb berberine for distinguishing BSA from HSA selectively. The probe itself has very weak fluorescenc...

Current researches on excreted detrimental chemical hazards successfully put forward the noxious effects of toxic metal ion and released harmful organic dyes (like TB and Rh-B) upon revelation into the environment. However, the exposure of Hg2+ ions is the most alarming sinister among all. In order to get rid of the littering deleterious effect, de...

A highly responsive and choosy patterning nanomaterial has been developed based on 5-methyl-1,3,4-thiadiazole-2-thiol fabricated silver nanoparticles with the simplicity of colorimetric performance. The assay is a trustworthy tool for the selective detection of glutathione (GSH), the mother of all antioxidant as well as a principle detoxifier in th...

The work highlighted interaction of harmalol, harmaline and harmine with human serum albumin by biophysical and biochemical assays. Presence of serum protein in the media negatively affects the cytotoxicity of the alkaloids. MTT assay indicates concentration dependent growth inhibitory effect of the alkaloids on A375, MDA-MB-231, HeLa, A549, ACHN a...

The detection and discrimination of serum albumins (SAs) has been transforming as a research work of keen interest to the scientists in recent times. This is in the root of foundation of more and more fluorescent probes to selectively identify and distinguish the SAs in the modern era of research. Fluorescence based sensors are preferably on high d...

The present manuscript reports a lucrative design of a colorimetric and ratiometric chemodosimeter, 9-O-(2, 4-dinitrobenzenesulfonyl) berberrubine (BER-S) with excellent water solubility for superb selective detection of sulfide through a color alteration from yellow to red with a well limit of detection (LOD) of 56 nM in CP buffer solution (10mM,...

In the present study, synthesis of two complexes, namely [Cu(L)Cl2] (1) and [Ni(L)Cl(H2O)2)]Cl (2), where L = piperidin-2-yl-N-(1-(pyridin-2-yl) ethylidene)methanamine were reported along with their characterization by spectroscopic techniques. The crystal structures were elucidated by single crystal X-ray diffraction technique. The structures of t...

We have reported the eco-friendly gold nanoparticles (AuNPs) from 15% aqueous leaf extract of therapeutic plant Nymphaea nouchali where leaf extract acts as both reducing and stabilizing agents. The generation of AuNPs was confirmed by UV–visible spectroscopy, FTIR, FE-SEM, DLS, and the size and morphology were further confirmed via TEM analysis (a...

Nanomaterials in different form have been thoroughly used in the area of pharmaceutics and medicine for drug delivery. The large scale of nanoparticles (NPs) synthesis from plant extract is much safe, cheap and eco-friendly. Here, we demonstrated a new, one-step, ultra-fast biosynthesis of gold nanoparticles (sc-AuNPs, 19.54 nm) by using aqueous Sp...

Triazole linked C-glycosyl pyrene fluorescent sensors 3 and 4 have been designed, synthesized and characterized for the purpose of detecting Au³⁺ ions. In citrate phosphate buffer, sensors 3 and 4 exhibited high selectivity and sensitivity in two different detection techniques (absorbance and fluorescence) in detecting Au³⁺ over wide variation of c...

A new Co(II) complex of general formula [Co(L) 2 ] has been synthesized from a NNO tridentate Schiff base ligand, 2-[(piperidin-2-ylmethylimino)-methyl]-phenol (L). The title complex is characterized by elemental, spectroscopic, antibacterial, and single crystal X-ray structural studies. X-Ray crystallography reveals that the complex shows a distor...

Nanomaterials are used to modify and improve the pharmacodynamic and pharmacokinetic properties of drug molecules of diverse types. The plant extracts are safe, beneficial and ecological for the synthesis of the large scale of nanomaterials. Herein, we report the new ultra fast silver nanoparticles (SNPs) synthesis via green route using aqueous Spa...

Chemical stabilized nanomaterials have enjoyed success as multifunctional therapeutic, diagnostic, catalytic properties and resolved a number of issues associated on interaction with biological systems. But they are unable to conquer the pricey synthetic route and production of chemical hazards. To triumph over this inadequacy, a one-step economica...

Herein, we report a rare pentacyclic N-fused hetrocycle synthesis via palladium catalyzed intramolecular C−H arylation along with the brief synthetic study of new type of bicyclic/tricyclic pyrrole rings. This pyrrole rings provided diversely substituted polycyclic nitrogen fused heterocycles from N-benzyl/N-benzyloxy pyrroles with good to excellen...

A sensitive platform for in vivo selective detection of the toxic element by a DNA stabilized biomolecule is of great importance. Particularly sensing of hydrazine in the presence of DNA is very important during cell damage and post-mortem. In this study, a new 9-O-(4-nitrobenzoyl) berberine analogue was designed and synthesized. The Calf thymus DN...

This study investigates the green synthesis of AgNPs from 1 mM aqueous AgNO3 using 10% leaf extract of Alstonia scholaris (Chhatim) for its wide antibacterial and medicinal properties. The synthesized AgNPs were duly characterized by UV–vis (UV–vis) spectrophotometry, dynamic light scattering, field emission scanning electron microscopy, transmissi...

This study looks into a safe, proficient and low-cost way for the preparation of novel silver nanoparticles by using 5% aqueous leaves extract of a medicinal plant, Marsilea quadrifolia (family: Marsileaceae) without using any external reducing and stabilizing agents. The synthesized AgNPs showed maximum UV-Vis absorbance at 435 nm due to surface p...

Nanocarrier mediated targeted delivery and biosensing in reproductive health care is a major exploratory domain. This work demonstrates the loading of silver nanoparticle (AgNP) inside the multiwalled Carbon nanotube(MWCNT) and their targetability to the intracellular part of the sperm cell for its further application in biosensing based infertilit...

Herein, we report an efficient synthesis of N-substituted pyrrole derivatives and their application to construct macrocyclic oxazocinone via a two-component coupling reaction followed by base mediated intramolecular cyclization. This methodology provides an easy two-step approach to constitute a library of fused pyrrolo-oxazocinone derivatives in g...

The green synthesis of nanoparticles has received increasing attention due to the growing demand to produce safe, cost-effective, and eco-friendly technology for nanomaterials synthesis. We report on the use of aqueous Croton bonplandianum (Family: Euphorbiaceae, Genus: Croton) leaves extract for the preparation of silver nanoparticles (AgNPs) with...

A mononuclear Cu(II) complex [Cu(L)Cl2] has been synthesized from a tridentate Schiff base ligand, piperidin-2-ylmethyl-pyridin-2-ylmethylene-amine (L). The single-crystal X-ray structure of the complex shows a square pyramidal geometry. The complex was tested against several bacteria and showed good antibacterial activities against almost all of t...

In this paper, the comparative binding behavior of antimalarial drug azure A, azure B and azure C with bovine serum albumin (BSA) has been studied. The interaction has been confirmed by multispectroscopic (UV, fluorescence, Fourier transform infrared (FT-IR), and circular dichroism) and molecular docking techniques. The experimental results show th...

Surface functionalized carbon nanoparticle composite in the form of heparin capped carbon nanoparticles composites are synthesized by dehydration of glucose. The said carbon nanoparticle composites are more stable with reduced cytotoxicity than the naked carbon nanoparticles. The said heparin capped carbon nanoparticle composites are found to have...

Drug delivery to prostate through conventional route is associated with pharmacokinetics based and side effects related problems. Alternate localized drug delivery route is a prerequisite which may offers many advantages over oral route of drug delivery to prostate. In male reproductive system, Vas deferens forms a continuous system with prostate,...

A non-toxic, hemocompatible, fluorescent probe as cobalt(II) based complex [Co(PS)4](ClO4)2 (1) (PS: N-Pyridylsalicylaldimine) was successfully synthesized and charecterised by single crystal X-ray diffraction studies. The structural analysis revealed that 1 exhibits intramolecular π- π interaction. The recognition ability of 1towards various amino...

In recent years, green synthesized nanoparticles from plant extract have drawn a great interest due to their prospective nanomedicinal application. This study investigates a proficient, safer and sustainable way for the preparation of AgNPs using medicinal plant Pongamia pinnata (family: Leguminoseae, Species: Pinnata) seeds extract without using a...

A new cadmium (II) complex, [Cd(4-Me-5-CHOIm)2Cl2] has been synthesized and characterized using single crystal X-ray diffraction study. The interaction of the complex with calf thymus DNA (CT-DNA) and double stranded RNA [polyinosinic·polycytidylic acid (poly(I)·poly(C)] has been investigated by various biophysical techniques. The interaction of th...

This paper reports the nanoscale interaction kinetics of three different inorganic nanoparticles (NPs) i.e., Zinc Oxide (ZnO), Titanium dioxide (TiO2) and Copper (Cu) NPs with viable human spermatozoa by physicochemical studies (UV-Vis Spectroscopy, ATR-FTIR), microscopic studies (SEM, HRTEM and fluorescence), and molecular thermodynamic studies (I...

Nanomaterial mediated drug delivery represents a highly promising technique while its selectivity for reproductive healthcare application still remains a challenge. Since the delicate structure and functional role of reproductive tissue and gametes require the use of biocompatible nanomedicine/devices that do not affect fertility or the development...

Nanomaterial mediated drug delivery represents a highly promising technique while its selectivity for reproductive healthcare application still remains a challenge. Since the delicate structure and functional role of reproductive tissue and gametes require the use of biocompatible nanomedicine/devices that do not affect fertility or the development...

Among gastrointestinal diseases, gallbladder stone associated inflammation is one of the major health concerns at ageing populations. The exact mechanism of gallstone formation is still an enigma. Gallstones are the biomineralized deposits in the gallbladder. Now this disease becomes one of the leading problems all over the world. To investigate th...

Amphotericin B and anidulafungin are widely used antifungal drugs for the treatment of systemic and serious mycoses. Amphotericin B is relatively toxic drug, has long been established. This study is first of its kind to systematically investigate the nature of binding to DNA, and to evaluate intercalation of AMP-B or ANIDULA with the aid of UV-Vis,...

A novel class of bridgehead nitrogen heterocycles, pyrido[1,2-a]pyrimidinium ions, has been readily synthesized by a two-step one-pot reaction in high yields (up to 93%). These ionic compounds are bench stable and moisture tolerant and have highly fluorescent properties (quantum yield up to 0.65). A characteristic bright bluish fluorescence was obs...

Berberine, palmatine and coralyne, the isoquinoline alkaloids distributed in many botanical families, are extensively investigated due to their potential therapeutic actions and clinical utilities. In this work, their binding characteristics to hemoglobin (Hb) were studied by UV-vis absorption spectroscopy, fluorescence spectroscopy, circular dichr...

A novel ligand bridged zinc(II) coordination polymer, [Zn(4-Me-5-CHOIm) 2 (HCOO)](ClO 4) has been synthesized and characterized by single crystal X-ray diffraction studies. The structural analysis revealed that the complex exhibits two types of 2D network by weak and strong hydrogen bonds. Interestingly, an extended supramolecular layer network has...

The complexation of three isoquinoline alkaloids berberine, palmatine and coralyne with α-, β-, and γ-CDs were studied by absorption, fluorescence, circular dichroism, NMR spectroscopy and microcalorimetric assay techniques. Their binding constant (K BH) values were determined by Benesi–Hildebrand equation. All the alkaloids formed 1:1 stoichiometr...

Polymerase chain reaction (PCR)-enhancing effect of gold nanoparticles (GNPs) has been reported earlier. Two opinions exist; one suggests that the enhancement arises due to an enrichment of heat transfer by high-conductivity, high-heat capacity GNPs while the other indicates that the PCR-enhancing effect of GNPs is mediated by surface interactions...

Integrase Interactor 1 (INI1/hSNF5) is a component of the hSWI/SNF chromatin remodeling complex. The INI1 gene is either deleted or mutated in rhabdoid cancers like ATRT (Atypical terratoid and rhabdoid tumor). INI1 is also a host factor for HIV-1 replication. INI1 binds DNA non-specifically. However, the mechanism of DNA binding and its biological...

The thermodynamics of the reaction of biogenic polyamines spermine, spermidine, putrescine and cadaverine with calf thymus DNA was studied by thermal melting, differential scanning calorimetry and isothermal titration calorimetry experiments. These results were supplemented by ethidium bromide displacement and circular dichroism experiments. Meltin...

Berberine, palmatine and coralyne, the isoquinoline alkaloids distributed in many botanical families, are extensively investigated due to their potential therapeutic actions and clinical utilities. In this work, their binding characteristics to hemoglobin (Hb) were studied by UV-vis absorption spectroscopy, fluorescence spectroscopy, circular dichr...

The thermodynamics of the interaction of two pharmaceutically important isoquinoline alkaloids berberine and palmatine with bovine and human serum albumin was investigated using calorimetric techniques, and the data was supplemented with fluorescence and circular dichroism studies. Thermodynamic results revealed that there was only one class of bin...

The health benefits stemming from green tea are well known, but the exact mechanism of its biological activity is not elucidated. Epicatechin (EC) and epicatechin gallate (ECG) are two dietary catechins ubiquitously present in green tea. Serum albumins functionally carry these catechins through the circulatory system and eliminate reactive oxygen s...

K562 cells are erythroleukemic cells derived from a chronic myeloid leukemia patient in blast crisis. Comparison of the genome from K562 cells and normal human genome has been very useful strategy, in uncovering eight genes, implicated in acute myeloid leukemia (AML). These genes carry mutations in K562 genome and the role of these mutations in the...

The interaction of the natural plant alkaloid and anticancer agent sanguinarine with tRNA(phe) has been investigated by spectroscopic and calorimetric techniques. Sanguinarine iminium binds to tRNA(phe) cooperatively; alkanolamine does not bind but in presence of large tRNA(phe) concentration, a conversion from alkanolamine to iminium occurs result...

Binding of two 9-O-(ω-amino) alkyl ether berberine analogs BC1 and BC2 to the RNA triplex poly(U)(•)poly(A)(*)poly(U) was studied by various biophysical techniques. Berberine analogs bind to the RNA triplex non-cooperatively. The affinity of binding was remarkably high by about 5 and 15 times, respectively, for BC1 and BC2 compared to berberine. Th...

Job plot for the complexation of BC, BC1 and BC2 with the RNA triplex. (TIF)

Stern-Volmer plots for the quenching of BC, BC1 and BC2 by RNA triplex. (TIF)

Optical properties of free and triplex bound alkaloid analogs. (DOC)

The binding of the phenothiazinium dye methylene blue to single stranded poly(riboadenylic acid) was investigated by spectroscopic and calorimetric techniques. The binding was cooperative and the affinity was of the order of 106 M−1 at 100 mM [Na+] as determined from absorbance, fluorescence and calorimetric studies. Ferrocyanide quenching studies...

The structural effects and thermodynamics of the DNA binding of six berberine analogues with alkyl chains of varying length and a terminal phenyl group at the C-13 position were investigated. All the analogues bound DNA noncooperatively in contrast to the cooperative binding of berberine. The binding affinity was higher and the effect of the chain...

The interaction of the phototoxic alkaloid coralyne with bovine and human serum albumins (BSA, HSA) was investigated. Absorbance and fluorescence quenching experiments revealed the formation of strong complexes. Based on the binding parameters calculated from Stern-Volmer quenching method, coralyne has higher affinity to BSA (~10(5) M(-1)) compared...

The anticancer drugs Adriamycin (ADR) and Daunomycin (DNM) of the anthracycline family are effective in treating a variety of cancers. Although their interactions with other cellular targets may play a role in the selective cytotoxicity of these drugs, it is generally believed that intercalation with DNA is essential for their activity. However, a...

Binding of the phenothaizinium dye thionine with four sequence specific deoxyribopolynucleotides, poly(dG-dC).poly(dG-dC), poly(dG).poly(dC), poly(dA-dT).poly(dA-dT), and poly(dA).poly(dT) has been investigated by means of thermal helix melting, isothermal titration calorimetry, and differential scanning calorimetry experiments. The binding affinit...

Difference spectrum of BSA and sanguinarine iminium (A) and alkanolamine forms (B). (TIF)

Interaction of the iminium and alkanolamine forms of sanguinarine with bovine serum albumin (BSA) was characterized by spectroscopic and calorimetric techniques. Formation of strong complexes of BSA with both iminium and alkanolamine forms was revealed from fluorescence quenching of sanguinarine. Binding parameters calculated from Stern-Volmer quen...

Sanguinarine is an anticancer plant alkaloid that can exist in the charged iminium and neutral alkanolamine forms. The thermodynamics of the interaction of the two forms with human serum albumin was investigated using calorimetric techniques, and the data supplemented with circular dichroism and spectrofluorimetric studies. The thermodynamic result...

The base specificity and energetics of DNA binding of the phenazinium dyes phenosafranine and safranine-O have been studied using various biophysical tools. The guanine-cytosine base specificity of both compounds was established from binding affinity values and competition dialysis results and also from circular dichroism, thermal melting, and calo...

Energetics of the binding of two known classical DNA intercalating molecules, ethidium and sanguinarine with four sequence specific polynucleotides, poly(dG-dC).poly(dG-dC), poly(dG).poly(dC), poly(dA-dT).poly(dA-dT), and poly(dA).poly(dT) have been compared under identical conditions. The binding of both the molecules was characterized by strong s...

To understand the structure-activity relationship of isoquinoline alkaloids, absorption, fluorescence, circular dichroism, and thermodynamics were employed to study the interaction of five C-9-ω-amino alkyl ether analogs from the plant alkaloid berberine with double-stranded calf thymus DNA. The C-9 derivatization resulted in dramatic enhancements...

The sequence selectivity of the DNA binding of the phenazinium dyes phenosafranin and safranin O have been investigated with four sequence-specific deoxyribopolynucleotides from spectroscopic and calorimetric studies. The alternating guanine-cytosine sequence selectivity of the dyes has been revealed from binding affinity values, circular dichroism...

In this study absorbance, fluorescence, circular dichroic spectroscopy, viscosity, thermal melting and calorimetric techniques were employed to understand the binding of the phenothiazinium dye, thionine, with deoxyribonucleic acids of varying base composition. Strong hypochromic and bathochromic effects and quenching of fluorescence were observed...

The binding of the known DNA intercalators methylene blue and quinacrine with four sequence specific polynucleotides, viz. poly(dG-dC).poly(dG-dC), poly(dG).poly(dC), poly(dA-dT).poly(dA-dT) and poly(dA).poly(dT), have been compared using absorbance, fluorescence, competition dialysis and thermal melting and the thermodynamic aspects of the interac...

There is compelling evidence that cellular DNA is the target of many small molecule anticancer agents. Consequently, elucidation of the molecular nature governing the interaction of small molecules to DNA is paramount to the progression of the rational drug design strategies. In this study, we have compared the binding and thermodynamic aspects of...

Design and synthesis of new small molecules binding to double-stranded RNA necessitate complete understanding of the molecular aspects of the binding of many existing molecules. Toward this goal, in this work we evaluated the biophysical aspects of the interaction of a DNA intercalator (proflavine) and a minor groove binder (hoechst 33258) with two...

Sanguinarine is a medically important plant alkaloid with remarkable pharmacological and biological activities. DNA binding is considered to be an important aspect in its mode of action. Isothermal titration calorimetric technique was used for the first time to derive the thermodynamic aspects of the interaction of the phototoxic and cytotoxic plan...

There is compelling evidence that cellular DNA is the target of many anticancer agents. Consequently, elucidation of the molecular nature governing the interaction of small molecules to DNA is paramount to the progression of rational drug design strategies. In this study, we have compared the binding and thermodynamic aspects of two known DNA-bindi...

A key step in the rational design of new RNA binding small molecules necessitates a complete elucidation of the molecular aspects of the binding of existing molecules to RNA structures. This work focuses towards the understanding of the interaction of a DNA intercalator, quinacrine and a minor groove binder 4',6-diamidino-2-phenylindole (DAPI) with...

The interaction of the protoberberine alkaloid palmatine with single and double stranded structures of poly(A) was studied by various biophysical techniques. Comparative binding studies were also performed with double stranded DNA, t-RNA, poly(C).poly(G), poly(U) and poly(C). The results of competition dialysis, fluorescence, and absorption spectra...

The cytotoxic plant alkaloid palmatine was found to bind strongly by partial intercalation to single stranded poly(A) structure with binding affinity (Ka) of (8.36+/-0.26) x 10(5) M(-1). The binding of palmatine was characterized to be exothermic and enthalpy driven with one palmatine for every two adenine residues. On the other hand, the binding t...