Mahmoud Sitohy

Zagazig University · Department of Biochemistry

This study evaluates the efficacy of discrete bacterial consortia in bioremediating sandy loamy soil deliberately contaminated with 20 mg/kg of chlorantraniliprole (CAP). It monitors alterations in total bacterial populations and CO2 emissions, tracking residual CAP levels through UV scanning and HPLC analysis. Six active bacterial degraders (four...

The 7S globulins (seed storage glycoprotein) isolated from soybean and chickpea seeds have the antifungal potential against the pathogenic fungus (Botrytis cinerea) causing gray mold in grapes assessing table grapes in vitro or postharvest by comparing with synthetic fungicide, Switch 62.5 WG. Conventional microbiological procedures estimated the i...

Appropriate knowledge of the parental cultivars is a pre-requisite for a successful breeding program. This study characterized fruit yield, quality attributes, and molecular variations of ten tomato cultivars during three consecutive generations under greenhouse conditions. Peto 86, Castle Rock, and Red Star cultivars showed the highest fruit yield...

The yellow polyphenolic pigment known as curcumin, originating from the rhizome of the turmeric plant Curcuma longa L., has been utilized for ages in ancient medicine, as well as in cooking and food coloring. Recently, the biological activities of turmeric and curcumin have been thoroughly investigated. The studies mainly focused on their antioxida...

Heavy metal accumulation and pathogenic bacteria cause adverse effects on aquaculture. The active surface of selenium (Se) nanoparticles can mitigate these effects. The present study used Se-resistant Bacillus subtilis AS12 to fabricate biological Se nanoparticles (Bio-SeNPs). The double-edged Bio-SeNPs were tested for their ability to reduce the h...

The main target of this work is to discover new protein fractions from natural resources with high antibacterial action. The 7S and 11S globulin fractions, as well as the basic subunit (BS), were isolated from lupine seeds (Lupinus termis), chemically characterized, and screened for antibacterial activity against seven pathogenic bacteria. SDS-PAGE...

The current study aims to efficiently utilize nanomaterials as an ecological protective tool to maintain a safe environment safe, preserve human health, and support societal welfare via improving water chemical quality. Practically, carbon nanotubes (CNT), as chemical adsorbents, were evaluated for their capacity to remove both lead and copper ions...

Background The world is facing an extraordinarily unprecedented threat from the COVID-19 pandemic triggered by the SARS-CoV-2 virus. Global life has turned upside down, and that several countries closed their borders, simultaneously with the blockage of life cycle as a result of the shutdown of the majority of workplaces except the food stores and...

Apposite energy is required for body activity. Energy is derived from the oxidation of various biomolecules like carbohydrates, lipids, and proteins. These bio-molecules in the proper amount are essential for the structural and functional activities of any living being. Certain vitamins and enzymes are also needed for the maintenance of biochemical...

Methylated soy protein (MSP) which is positively charged with enhanced hydrophobicity may have antiviral action. This study is verifying if MSP can act inhibit H5N1 inside an animal model. Five groups of Hubbard chicks were challenged at the 25th day of the experiment with AIV virus (H5N1; 0.1 × 10 5 EID 50 /mL); 1 group did not receive any treatme...

This study investigated the ideal conditions for achieving fully deacetylated chitosan from shrimp and crab shell detritus. The deacetylation method consisted mainly of two steps, heating the decolorized chitin at 100 ºC for two, four, and six hours, then cool-stirring at 200 rpm for zero, two, and four hours. The maximum degree of deacetylation (D...

Sixty-six (66) Staphylococcus bacterial isolates were withdrawn from separate clinical samples of hospitalized patients with various clinical infections. Conventional bacteriological tests identified the species of all isolates, and standard microbiological techniques differentiated them into CoPS or CoNS. Their biofilm development was followed by...

Assessment of gene expression is an essential and sensitive indicator of the biological activity and function of a certain gene. The expression profile of R2R3 MYB gene (Solyc02g067760) was investigated at ten development stages of tomato fruits (Lycopersicon esculentum) of Micro-Tom cultivar using three different approaches; quantitative RT-PCR, s...

The consumption of plant-based dairy alternatives has increased rapidly around the world as a result of numerous positive health effects. Little information is available about the potential use of watermelon seed milk in the manufacture of yoghurt. The present study was undertaken to investigate the remedial action of yoghurt enriched with watermel...

The emergence of coronavirus disease 2019 (COVID-19) pandemic in Wuhan city, China at the end of 2019 made it urgent to identify the origin of the causal pathogen and its molecular evolution, to appropriately design an effective vaccine. This study analyzes the evolutionary background of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV...

In recent years, significant progress has been achieved in genome editing applications using new programmable DNA nucleases such as zinc finger nucleases (ZFNs), transcription activator-like endonucleases (TALENs) and the clustered regularly interspaced short palindromic repeats/Cas9 system (CRISPR/Cas9).These genome editing tools are capable of ni...

The use of nanomaterials alone or in composites with proteins is a promising alternative to inhibit pathogenic bacteria. In this regard, this study used seed proteins from both fenugreek (Trigonella foenum-graecum L.) (FNP) and mung bean (Viga radiate) (MNP), with silver nanoparticles (Ag-NPs) and nanocomposites of either Ag-NPs plus FNP (Ag-FNP) o...

Taxadiene synthase (TDS) is the rate-limiting enzyme of Taxol biosynthesis that cyclizes the geranylgeranyl pyrophosphate into taxadiene. Attenuating Taxol productivity by fungi is the main challenge impeding its industrial application; it is possible that silencing the expression of TDS is the most noticeable genomic feature associated with Taxol-...

Introduction: Biofungicides with low toxicity and high efficiency are a global priority for sustainable agricultural development. Phytohormone salicylic acid (SA) is an ancient medicine against various diseases in humans and activates the immune system in plants, but little is known of its function as a biofungicide. Objectives: Here, Fusarium o...

The present study examines the impacts of supplementing yogurt with 1% whey protein concentrate (WPC), Ca-caseinate (Ca-CN) and Spirulina platensis on the physiological performance of V-line rabbits receiving diets containing yogurt (at a dose of 5 g/kg body weight/day) and the different meat quality aspects. The results show that fat content was h...

Crustacean waste is one of the most severe issues, posing significant environmental and health risks. This study aims to improve managing marine waste by isolating chitosan from Procambarus clarkii by devising a new methodology, incorporating technical steps, e.g., washing, decolorization and deacetylation under a reflexive condenser and dialysis p...

Due to their beneficial, stimulating impact on plant growth, productivity, and alleviating environmental stresses, protein hydrolysates have recently received increasing attention as a possible substitute. This investigation aimed to explore the effects of foliar application of papain-produced whey protein hydrolysates (WPH) on the yield attributes...

A new preservation approach is presented in this article to prolong the lifetime of raw chicken meat and enhance its quality at 4 °C via coating with highly soluble kidney bean protein hydrolysate. The hydrolysates of the black, red, and white kidney protein (BKH, RKH, and WKH) were obtained after 30 min enzymatic hydrolysis with Alcalase (E/S rati...

Cowpea seed protein hydrolysates (CPH) were output from cowpea seeds applying alcalase® from Bacillus licheniformis. CPH with an elevated level of hydrolysis was fractionated by size exclusion chromatography (SEC). Both CPH and SEC-portions showed to contain antimicrobial peptides (AMPs) as they inhibited both Gram-positive bacteria, such as Lister...

Lettuce (Lactuca sativa) was grown using a foliar spray with whey protein hydrolysate (WPH) as opposed to normal nitrate fertilization. Lettuce juice was prepared from lettuce cultivated without any fertilization, nitrate fertilization, or WPH. Sixty weaned, 4-week-old male V-line rabbits with an average 455 ± 6 g body weight were randomly divided...

Papain and pepsin-hydrolyzed whey protein (PAH and PEH, respectively) were prepared and characterized for its degree of hydrolysis, chemical constituents (amino acid and peptides) and antioxidant activity. A field experiment was conducted at El Salheya El Gedida City, Sharqia, Egypt, during the seasons 2019 and 2020, to investigate the biological a...

This study aimed to produce a palatable functional cucumber juice: non-supplemented cucumber juice, control juice (J-con), and supplemented with different herbal extracts. Four juices were prepared and evaluated, as supplemented with the ethanolic extracts of cinnamon, mint, ginger, and clove denoting as; J-cin, J-min, J-gin, and J-clov, respective...

BACKGROUND Cucumber plants suffer from a serious threatening disease, downy mildew, throughout the growing seasons irrespective of the weather temperature. The causal agent, Pseudoperonospora cubensis, tends to evolve rapidly upon sequential applications of chemical fungicides and generate new progeny possessing tolerance to such fungicides. Glycop...

Methylated soy and chickpea proteins (MSP and MCP, respectively), and 11S globulin (a soy protein fraction), are characterized by net positive charges and a more hydrophobic nature, nominating them as antiviral proteins. Under greenhouse conditions, potato plants (cv. Spunta) mechanically infected with potato virus Y (PVY) and potato virus X (PVX)...

Bio iron (II) nanoparticles can be natural preservative and a source of available iron. Bacillus subtilis ML6 supernatant reduced FeCl3, producing biological ferrous nanoparticles (Bio-Fe (II)NPs). Bio-Fe (II)NPs were crystalline, absorbed UV at the range 250–350 nm, inhibited pathogenic microbes and exhibited considerable scavenging activity. Yogu...

The aim of the work was to study the effect of adding cucumber pomace powder (CP) to soft wheat flour used in noodle manufacture. CP is a rich source of minerals and fibres, having high contents of polyphenols and flavonoids were 1.6 mg g⁻¹ GAE and 0.65 mg g⁻¹ QE, respectively, besides its capability to inhibit bacterial and fungal growth and to sc...

The impact of partial replacement of fish meal with methylated soy protein isolates (MSPI) on fish performance was assessed in 10 weeks feeding trial using Nile tilapia,Oreochromisniloticusfingerlings (n = 225) with average initial weight 18.51 ± 0.05 g/fish. The effects on the growth, histopathological condition of certain organs, economic efficie...

The current study investigates the capacity of a lipolytic Lactobacillus paracasei postbiotic as a possible regulator for lipid metabolism by targeting metabolic syndrome as a possibly safer antiobesity and Anti-dyslipidemia agent replacing atorvastatin (ATOR) and other drugs with proven or suspected health hazards. The high DPPH (1,1-diphenyl-2-pi...

Native egg albumin (NEA) was isolated from hen eggs and hydrolyzed by pepsin to produce hydrolyzed egg albumin (HEA). HEA was chemically characterized and screened for its antibacterial activity against 10 pathogenic bacteria (6 Gram (+) and 4 Gram (−)). The SDS-PAGE pattern of NEA showed molecular weights of hen egg albumin subunits ranging from 3...

Phycocyanin isolated from Anabaena biomass was in‐vitro assayed for its antioxidant activity against DPPH (2,2‐diphenyl‐1‐picrylhydrazyl) and ABTS [2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid)] free radical, revealing maximum activities amounting to 77 and 80% at 1,000 µg/ml and SC50 values about 96 and 111 µg/ml, respectively. A biologic...

The potentiality of Stevia leaves and turmeric roots as remedies against diabetes mellitus type 2 was tested in this study. Stevia leaves and turmeric roots were extracted with ethanol:water (80:20 v/v) and analyzed by HPLC. Turmeric extract (TUE) was rich in; curcumin, gallic acid, and eugenol. Stevia extract (STE) contained 28 known compounds, in...

The present study aims to elucidate the microbiota of Egyptian mish cheese and milk, to isolate new strains and to study their biochemical properties relevant to their potential application in food and dairy industry. Among the isolates from Egyptian mish cheese there were two strains identified to be Lactobacillus fuchuensis DSM 14340T DSM and Lac...

Delila and Rosea1 anthocyanin accumulation genes were subjected to bioinformatics analysis. Delila protein has 56 - 69% similarity with different anthocyanin-rich plants, while Rosea1protein has 83 - 87% with anthocyanin-rich plant proteins. This study aimed at transferring Delila and Rosea1 genes from transgenic Micro- tom tomato cultivar to Money...

Minced beef is a very perishable food product, due to its vulnerability to microbial contamination and its fast quality deterioration. In the current study, the biological efficiency of different concentrations (0, 50 and 100 µg g−1) of the antibacterial catfish glycoprotein (CFG) was estimated as a possible improver of the storability and safety o...

Potato plants are liable to PVY infection without efficient control. Therefore, they were cultivated under greenhouse and open field conditions, artificially infected with PVY and then treated after 15 days of infection with native lactoferrin (LF) and native β-lactoglobulin (BL) and their esterified forms, MLF (methylated lactoferrin) and BLM (met...

Endophytic fungi have been considered as a repertoire for bioactive secondary metabolites with potential application in medicine, agriculture and food industry. The biosynthetic pathways by fungal endophytes raise the argument of acquisition of these machineries of such complex metabolites from the plant host. Diterpenoids “Taxol” is the most effec...

Pumpkin seed protein hydrolysate (PH) is a biostimulant product that contains high antioxidant activities, peptides and amino acids. This biostimulant is able to improve plant tolerance to abiotic stresses, including salinity. A pot experiment was conducted during summer season of 2018, with the aim of determining growth and productivity, physio-bi...

Background Since the emergence of the pandemic novel pneumonia (COVID-19) disease in Wuhan city in China in November 2019, it is becoming holistically urgent to discover and definitely determine the potential origin of causative virus of this disease, SARS-CoV2 to understand its pathogenic action an better design proper remedies. Methods Using bio...

The authors have withdrawn this preprint due to author disagreement.

Starches are valuable biopolymers with several functional and technical applications. Physical and chemical modifications are needed to enhance starch functionality. Modified starches exhibit high technological properties in food and non‐food applications. Modified starches are designed to overcome some physicochemical shortcomings of native starch...

Poultry enterprises have sustained rapid development through the last three decennaries. For which reason, higher utilization of antibacterial, either as therapeutic or growth promoting agents, has been accepted. Owing to the concern of developing bacterial resistance among populations towards antibiotic generations, accumulation of antibacterial r...

BACKGROUND Alternaria tenuissima was isolated from infected fig fruit and molecularly identified by rRNA gene sequencing. The objective of the current work was to test the inhibitory effect of vicilin as a glycoprotein, isolated from chickpea, against the fungus A. tenuissima, isolated from fig fruit, in vitro and in situ, to estimate its potential...

The current research aimed to estimate the effect of dietary supplementation with glycinin isolated from soybeans on the growth performance, carcass traits, and selected blood metabolites of broiler chicks. A total of 200 1-wk-old broiler chicks were administered diets without glycinin (control treatment) or diets supplemented with 3 concentrations...

Taxol is one of the potential anticancer drugs; however, the yield of Taxol and its cytotoxicity are common challenges. Thus, manipulating the Taxol biosynthetic pathway from endophytic fungi, in addition to chemical modification with biocompatible polymers, is the challenge. Four fungal isolates, namely, Aspergillus flavipes, A. terreus, A. flavus...

The impact of partial replacement of fish meal with methylated soy protein isolates (MSPI) on fish performance was assessed in 10 weeks feeding trial using Nile tilapia,Oreochromis niloticus fingerlings (n = 225) with average initial weight 18.51± 0.05 gm/fish. The effects on the growth, histopathological condition of certain organs, economic effi...

Buffalo skim milk retentate was hydrolyzed with papain for 4 h (enzyme:substrate, 1:200), resulting in a retentate hydrolysate (RH) with a degree of hydrolysis of 23%. We then investigated the potential hepatoprotective activity of RH at 250 and 500 mg/kg of body weight per day on carbon tetrachloride (CCl4)-induced oxidative stress in albino rats....

Crude, phenolic-rich extracts (CPREs) were isolated from different sources, such as Hibiscus sabdariffa (H. sabdariffa), Brassica oleracea var. capitata f. rubra (B. oleracea) and Beta vulgaris (B. vulgaris) and characterized. These CPREs showed potential antibacterial and antifungal activities. H. sabdariffa CPRE (HCPRE) is the most potent, as it...

Native seed proteins(Globulin) could be modified to modified seed globulin (MSG) of net positive charges on their both 7S and 11S subunits by biochemical methylation of side chains of amino acid residues.The positively charged MSG easily attach negatively charged phospholipid of bacterial membranes , making membrane deformations and deenergized die...