Mahmoud Hamdy

Animal Health Research Institute · Brucellosis Research Department

Background and Aim: Given the rise in stray and imported dogs in Egypt over the past 5 years, it is surprising that no report of Brucella canis infection in dogs or humans has been documented in Egypt’s published papers. This study aimed to detect the presence of antibodies against the rough (B. canis) and smooth Brucellae among dogs in Egypt and t...

Abstract Background and Aim: Given the rise in stray and imported dogs in Egypt over the past 5 years, it is surprising that no report of Brucella canis infection in dogs or humans has been documented in Egypt's published papers. This study aimed to detect the presence of antibodies against the rough (B. canis) and smooth Brucellae among dogs in E...

In the current study, 14 Brucella suis biovar 2 (B. suis bv 2) strains isolated from slaughter pigs in Cairo were sequenced using Illumina technology to investigate genetic diversity, antimicrobial resistance (AMR) genes, and virulence-associated determinants. These strains were the first B. suis bv 2 isolates from Egypt. To place them in a global...

Brucellosis is one of the most common neglected zoonotic diseases globally, with a public health significance and a high economic loss in the livestock industry caused by the bacteria of the genus Brucella. In this study, 136 Egyptian B. melitensis strains isolated from animals and humans between 2001 and 2020 were analyzed by examining the whole c...

Background Brucella suis is a zoonotic pathogen with a serious impact on public health and the pig industry worldwide. Information regarding B. suis in pigs in Egypt is scarce. This study aimed to investigate the prevalence of B. suis in slaughtered domestic pigs at El-Basatin abattoir in Cairo, Egypt. A total of 1,116 domestic pigs slaughtered in...

For the aim of genotypic fingerprinting of Brucella melitensis bv3 isolated from different ruminant species in Kafrelsheikh governorate, Egypt, a multilocus variable-number tandem-repeat analysis (MLVA 16) has been approached. The MLVA 16 was performed on 41 B. melitensis bv3 isolates identified by bacteriological and molecular techniques. Thirty-o...

Brucellosis is among the most prevalent zoonotic infections in Middle Eastern and North African (MENA) countries, critically impacting human and animal health. A comprehensive review of studies on antibiotic susceptibility and therapeutic regimes for brucellosis in ruminants and humans in the MENA region was conducted to evaluate the current therap...

To investigate the incidence of the virulence genes among predominant Brucella strains in infected cattle, buffaloes, sheep, goats, and camels as well as in humans in Egypt, a total of 263 samples (85 milk, 167 tissue, 11 whole blood samples), yielded 140 (53.2%) Brucella isolates. Confirmation of Brucella isolates was carried out by conventional b...

This study aimed to evaluate two PCR-based DNA fingerprinting techniques: Enterobacterial repetitive intergenic consensus (ERIC) and Random amplification polymorphism DNA (RAPD), for genotyping the Brucella isolates, as alternative rapid tools for epidemiological tracing and investigation of brucellosis in Egypt. We recovered Brucella isolates (n=2...

This is an open-access article under the terms of the Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium provided the original work is properly cited. Abstract Aims: This study aimed to identify the genotypic fingerprinting of Brucella melitensis biovar 3 isolates from ruminants in Kafr El-Sheikh,...

Brucellosis, caused by the bacteria of the genus Brucella, is one of the most neglected common zoonotic diseases globally with a public health significance and a high economic loss among the livestock industry worldwide. Since little is known about the distribution of B. abortus in Egypt, a total of 46 B. abortus isolates recovered between 2012–202...

Brucellosis is a major constraint to livestock production that is still enzootic in livestock in many developing countries including Egypt. This study was conducted with the general objective of establishing the bacteriological status of bovine brucellosis in 15 governorates in Egypt during 2020-2021 to determine the circulating Brucella species on...

Aims: This study aimed to identify the genotypic fingerprinting of B. melitensis biovar 3 isolates from ruminants in Kafr El-Sheikh, Egypt, to compare with other peers globally, and to highlight the epidemiology and potential causes of brucellosis control failure. Methods and results: A multilocus variable-number tandem-repeat analysis (MLVA 16)...

Brucellosis is a highly contagious and incapacitating disease of humans, livestock and wildlife species globally. Treatment of brucellosis in animals is not recommended, and in humans, combinations of antibiotics recommended by the World Health Organization are used. However, sporadic antimicrobial-resistant (AMR) isolates and relapse cases have be...

Brucellosis is a widespread disease in Egypt which cause huge economic losses in the dairy industry. The present study aims at isolating and identifying Brucella (B.) spp. circulating in bovine and buffalo dairy herds kept at farmers houses in four districts of the Delta region of Egypt. One hundred and five tissue specimens were collected from ser...

Brucellosis is a common zoonotic disease in Egypt. However, there are limited data available on the genetic diversity of brucellae circulating in Egypt and other Mediterranean areas. One hundred and nine Brucella (B.) strains were isolated from different animal species in thirteen Egyptian governorates. Multi-locus variable number tandem repeats (V...

Supplementary Materials: Figure S1: The similarity matrix showing the similarity percentages and the maximum and minimum number of identical or different VNTRs within the cluster and between the two distinguished clusters of 49 B. melitensis isolates recovered from animal in Egypt. Figure S2. The similarity matrix showing the similarity percentag...

Abstract This study was carried out to investigate the effect of microwave radiation on the viability of Brucella melitensis in contaminated cattle milk through establishing a correlation between milk volumes, microwave exposure time, measured Celsius temperature and viable counts of B. melitensis in inoculated milk. Microwave appliances were used...

In this study, we compared the role of hydrogen peroxide (H 2 O 2) alone and in a compound mixture of H 2 O 2 and silver Nanoparticles (AgNs) on the elimination and inhibition of Brucella melitensis in contaminated milk. The compound product of H 2 O 2 and AgNs and hydrogen peroxide alone were applied in different concentrations of the (17 ml /L-10...

This study aimed to investigate the levels of acute phase proteins, antioxidants and trace elements in camels naturally infected with Brucella species. One hundred twenty sex blood samples were collected from camels (males, n = 58 and females, n = 68). Haptoglobin, fibrinogen, superoxide dis-mutase (SOD), glutathione peroxidase (GPx), ascorbic acid...

La brucelosis es una zoonosis altamente contagiosa de importancia para la salud pública y para el rendimiento económico tanto en países endémicos como en países no endémicos. La brucelosis se identificó por primera vez en la región del Mediterráneo y se cree que posteriormente se extendió por todo el mundo. En los humanos, se asocia a una escasa hi...

Preface: Brucellosis is an endemic disease in the Mediterranean Basin but accurate epidemiological data are not available for most of the area’s countries, despite several national and international surveys. This is the first publication to compiles and describe the historical and current status of brucellosis in all Mediterranean countries. Thirty...

La brucellose est une zoonose hautement contagieuse dont l’impact sur la santé publique et la performance économique des pays endémiques et non endémiques est considérable. Historiquement, la brucellose a été reconnue pour la première fois dans la région méditerranéenne à partir de laquelle elle se serait propagée dans le monde entier. La brucellos...

ARTICLE INFO Evaluation of the real-time PCR, rose bengal test (RBT), competitive ELISA, and complement fixation test (CFT) was done on 335 camels sera. Real-time PCR, classified 335 camel serum samples to 268 (80%) as positive and 67 (20%) as negative. Real-time PCR, using species specific primers, distinguished 94/104 serum samples due to B. abor...

Genotypic characterization of Brucella Spp. isolated from sheep and goats Ashraf A. Abdeltawab1, Mohsen A. Agag2, Mahmoud E. Hamdy3, Gamal Wareth4,5, Fatma I. El-Hofy1, Mohamed M. Ramadan1 1- Dept. of bacteriology, Immunology and Mycology Benha Univ. 2- Dept. of Theriogenology Benha Univ. 3- Animal Health Institute, Dokki, Giza. 4- Dept. of Patholo...

The current study involved detection of three virulence genes (bvfA, virB, ure) by PCR in 52 isolates of Brucella melitensis biovar 3, recovered from different animal species (28 sheep, 10 goats, 9 cattle and 5 buffaloes). Of the 52 B. melitensis strains; 48 (92.3%) isolates carried bvfA genes, 51 (98.1%) isolates had virB genes and 50 (96.2%) isol...

Brucellosis is a major infectious zoonotic disease affecting animal and human. In animal, the disease is causing great economic losses such as abortion, still birth as well as infertility in both male and female. A cross sectional study carried out from November 2016 to May 2017 to estimate the seroprevalence of brucellosis in sheep and goats in Be...

Brucellosis is a major infectious zoonotic disease affecting animal and human. In animal, the disease is causing great economic losses such as abortion, still birth as well as infertility in both male and female. A cross sectional study carried out from November 2016 to May 2017 to estimate the seroprevalence of brucellosis in sheep and goats in Be...

Evaluation of the real-time PCR, rose bengal test (RBT), competitive ELISA, and complement fixation test (CFT) was done on 335 camels sera. Real-time PCR, classified 335 camel serum samples to 268 (80%) as positive and 67 (20%) as negative. Real-time PCR, using species specific primers, distinguished 94/104 serum samples due to B. abortus, 4/10...

International Conference for Brucellosis Research, Brucella2008, Veterinary Laboratory Agency (VLA London, 10-13/9/2008, Abstract code P. 63.

A total number of 264 goats, located on a farm at Ai-Shamal city in the north of Qatar, were examined for five consecutive examinations. All animals belonging to this farm were serologically screened for brucellosis; positive animals were isolated and transferred to a remote farm for slaughtering, while negative ones were examined after a period of...

BALB/c mice were immunized with live rough Brucella abortus RB51 or smooth Brucella melitensis Rev. 1 vaccines and challenged with a B. melitensis field strain. Protection was assessed by a variety of serological tests and recovery of vaccinal and challenge strains by culture. Mice vaccinated with RB51 gave negative results in the conventional sero...

One hundred and three milk samples were collected from 52 cows, 21 ewes, 18 goats and 12 camels. The animals tested positive to at least one of the following: (1) standard tube agglutination test (SAT); (2) Rose Bengal plate test (RBPT); (3) milk ring test (MRT). All milk samples were examined by culture and single-step polymerase chain reaction (P...

General Meeting American Society for Microbiology, (ASM) Call For Abstracts 102nd , Salt Lake City, Utah, USA Ab. No. 972. (2002).

An evaluation of the polymerase chain reaction (PCR) for detection of Brucella melitensis DNA in bovine and ovine semen was performed. Since semen contains different components that inhibit PCR amplification, a protocol was used to purify Brucella-DNA from bovine and ovine semen samples prior to conducting amplification of the targeted DNA. When se...

In this study, 400 blood samples were collected from apparently healthy animals (100 cows, 100 buffaloes, 100 sheep and 100 pigs) for screening of serological prevalence of Y. enterocolitica agglutinins. 172 (43 %) strongly displayed antibodies against Y. enterocolitica srovar O:3 and/or O:9. Among these, Yersinia positive sera 164 (41 %) were inde...

In this study, 400 blood samples were collected from apparently healthy animals (100 cows, 100 buffaloes, 100 sheep and 100 pigs) for screening of serological prevalence of Y. enterocolitica agglutinins. 172 (43 %) strongly displayed antibodies against Y. enterocolitica srovar O:3 and/or O:9. Among these, Yersinia positive sera 164 (41 %) were inde...