Mahmoud Hadwan

Mahmoud Hadwan
University of Babylon · Department of Chemistry

Professor of Biochemistry 2018

About

58
Publications
28,343
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706
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Additional affiliations
October 2009 - January 2019
University of Babylon
Position
  • Professor (Full)
October 2005 - present
University of Babylon
Position
  • Professor (Full)

Publications

Publications (58)
Article
Spectrophotometric methodologies have been used to assess glutaminase activity, for which coloured complexes have been developed that measure spectrophotometry across the visible spectrum using different reagents. The present paper describes a precise, simple and reliable procedure for quantifying glutaminase activity, which is a key enzyme in glut...
Article
Cellulase is a microbial enzyme responsible for degrading the β-1,4 glycoside bond in polysaccharide cellulose, which is abundant in various animal foodstuffs. Cellulase is an important industrial enzyme used for various purposes, including biopolishing textile fibers, softening garments, biostoning denim fabric, and removing excess color from text...
Article
Full-text available
Catalase is an antioxidantenzyme existing in most of the animalcells; it defends them from oxidative damage by catalyzing the rapid decay of hydrogen peroxide in two kinds of reactions. The aim of the present study was to assess the catalase activity in normal subjects and patients whom suffer from diabetes mellitus. Serum catalase activity was ass...
Article
Full-text available
Sulfhydryl oxidase was studied using a spectrofluorometric assay. The current protocol operates by using a combination of hemoglobin (HB) and hematin (HT) as a peroxidase mimic to catalyze the H2O2-dependent oxidation of thiamine. The response surface methodology (RSM) is used to optimize the new method. The current method is very accurate, sensiti...
Article
This assay elucidates an accurate, simple, and precise protocol to quantify the activity of homocysteine thiolactonase (HTase). To establish HTase activity, the enzyme samples were incubated with a 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, which contained suitable concentrations of the homocysteine thiolactone as a substrat...
Article
This article presents a reliable, effective and easy procedure for measuring glutathione peroxidase (Gpx) activity. Enzyme samples were incubated with phosphate buffer, which included the appropriate concentrations of glutathione (GSH) and peroxide as substrates, to determine Gpx activity. In the CUPRAC method, the CUPRAC reagent Cu(Nc)22+ was adde...
Preprint
Full-text available
Sulfhydryl oxidase was studied using a spectrofluorometric assay. The current protocol operates by using a combination of hemoglobin (HB) and hematin (HT) as a peroxidase mimic to catalyze the H2O2-dependent oxidation of thiamine. The response surface methodology is used to optimize the new method (RSM). The current method is very accurate, sensiti...
Article
Full-text available
Background: Oxidative stress is an imbalance between free radical's production and the body's ability to counteract or detoxify their harmful effects through neutralization by antioxidants, oxidative stress is thought to be involved in the pathogenesis of diabetic nephropathy. One of the key enzymatic antioxidants is glutathione peroxidase (GPx),...
Article
Full-text available
Background: Accurate estimation of Prx activity poses many complications and interferences. The present protocol is free of interference and provides an effective alternative for the assessment of peroxide with high sensitivity. The assay can be used in clinical pathology laboratories since it is simple, rapid, and inexpensive. The systematic reag...
Article
Full-text available
A novel kinetic method for measuring catalase activity in biological samples was evaluated. The principle of the current method is based on the oxidation effect of unreacted hydrogen peroxide (H2O2) on pyrogallol red (PGR) using the catalytic effects of molybdenum. The decrease in the absorbance of PGR in the presence of H2O2 with time from 0.5 to...
Preprint
Full-text available
This essay presents a reliable, effective and easy procedure for measuring glutathione peroxidase activity (Gpx). The enzyme samples were incubated with phosphate buffer, which included appropriate concentrations of glutathione and peroxide as substrates, to determine the Gpx activity. After a sufficient incubation time, the CUPRAC reagent (Cu(Nc)...
Article
The current study describes a simple, precise, and accurate protocol for measuring the activity of catalase, which controls the rate-limiting step of the dissociation of hydrogen peroxide reactions. The current protocol assesses catalase activity by incubating catalase samples with suitable concentrations of hydrogen peroxide dissolved in a phospha...
Article
The current study describes a simple spectrophotometric protocol for the assessment of peroxiredoxin (Prx) activity and demonstrates its reproducibility, accuracy, and precision. The dissociation of organic or inorganic peroxide represents the rate-limiting step for reactions regulated by the Prx enzyme. In the current protocol, Prx enzyme activity...
Article
Full-text available
Background Catalase is a vital antioxidant enzyme that dismutates H2O2 into water and molecular oxygen. Many protocols have been developed to measure catalase enzyme activity. spectrophotometric methods are the most common assays that used to assess catalase enzyme activity. Methods Because the rate-limiting step during catalase enzyme activity de...
Article
Full-text available
Background: Uric acid (UA) is crucial for sperm metabolism as it protects seminal plasma against oxidative damage. Zinc also plays a central role in sperm metabolism. The current study was designed to investigate the role of zinc supplementation on qualitative and quantitative properties of seminal fluid, in parallel with the UA level and urate pa...
Article
Full-text available
Background: The liver is the major organ in the body and it is considered as an important organ for protein manufacture and detoxification. Liver health is evaluated by study liver function (LFTs) which include TSB, AST, ALT and ALK. The current study is an attempt to understand the correlation between morbid obesity and liver function enzymes. Met...
Article
Full-text available
A novel, simple, and accurate colorimetric assay was established for assessments of catalase activity in biological fluids and tissues. H2O2 dissociation rates are directly proportional to catalase activity, and the principle of the present assay is based on reactions of ammonium metavanadate with H2O2 under acidic conditions. The resulting reducti...
Article
Herein, we describe a simple spectrophotometric method for the measurement of peroxiredoxin activity and demonstrate reproducibility, accuracy, and precision. In these experiments, peroxiredoxin activity was measured by incubating enzyme samples with phosphate buffer solution containing suitable concentrations of the substrates 1,4-dithio-DL-threit...
Article
Full-text available
Background: The liver is the major organ in the body and it is considered as an important organ for protein manufacture and detoxification. Liver health is evaluated by study liver function (LFTs) which include TSB, AST, ALT and ALK. The current study is an attempt to understand the correlation between morbid obesity and liver function enzymes. Met...
Article
Full-text available
This article focus on synthesis of gold nano-particles using Iraqi mentha pulegium L, the prepared gold nanoparticles were investigated using UV-visible spectroscopy, X-rays diffraction, and scanning electron microscopy. The activity of the prepared gold nanoparticles was investigated via screening its biological and antioxidant activity. The obtai...
Article
Full-text available
Background: The details of a precise, accurate, and sensitive spectrophotometric method for measuring catalase activity are presented here. The assay was established for biological samples and depends on the rapid formation of a stable and colored carbonato-cobaltate (III) complex. Samples exhibiting catalase activity are incubated with hydrogen p...
Article
Full-text available
The aim of the study is to valuation some biomarker biochemical parameters that play important role in evaluating functions renal for hypertensive. The study was obtained. Included ninety-one. 35(38.46%) of the subjects were males and 56 (61.54%) were females. forty-two (42) subjects were selected as control and forty nine (49) suffered only from e...
Article
Medicinal plants are documented as supplies of usual antioxidants that can defend biological tissues from oxidative damage. In current study, the antioxidant status and antibacterial activities of gold nanoparticles (AuNPs) that synthesised by utilizing Mentha pulegium were estimated. Gold nanoparticles (AuNPs) have many potential applications in b...
Article
Full-text available
This work involves direct doping of Zn(CH3COO)2·2H2O with both single-walled carbon nanotubes (SWCNTs) and multi-walled carbon nanotubes (MWCNTs) at 60 ºC. The produced composites of ZnO/SWCNTs and ZnO/MWCNTs were investigated using X-rays diffraction patterns, atomic force microscopy and Fourier transform infrared spectroscopy. From the results, i...
Article
Full-text available
Introduction: Catalase is a principal constituent of the antioxidant system that attenuates the oxidative stress, which is ubiquitously associated with several types of pathological disorders. Aim: This paper describes a discontinuous assay for the assessment of catalase activity using the titanium tetrachloride/ sulfuric acid reagent. Materia...
Article
Full-text available
Background:Vitamin E is one of four fat-soluble vitamin with antioxidant property consists of tocopherols and tocotrienols with α-tocopherol the most bioactive member of this family. Aim: This study aims to assess always almost each potential deals withthe effect of consuming vitamin E on seminal fluids properties in asthenospermic patients. Method...
Article
Full-text available
A thiol group plays an essential role in sperm metabolism and the antioxidative defense state. Zinc is the second most abundant element in the human body, following iron. The present study was conducted to study the effect of zinc supplementation on the characteristics of semen along with thiol and thiol-related enzymes in semen of asthenospermic p...
Article
Full-text available
Introduction: Diabetes is a disease characterised by poor glycaemic control and development of various complications with age. Diabetic complications include development of nephropathy as well as other complications. Aim: The study was aimed to elucidate the consequences of diabetic nephropathy on erythropoietin levels and microalbuminuria. Materia...
Article
Full-text available
This work involves direct doping of Zn(CH3COO)2·2H2O with both single-walled carbon nanotubes (SWCNTs) and multi-walled carbon nanotubes (MWCNTs) at 60 ºC. The produced composites of ZnO/SWCNTs and ZnO/MWCNTs were investigated using X-rays diffraction patterns, atomic force microscopy and Fourier transform infrared spectroscopy. From the results, i...
Article
Full-text available
A novel, simple, and accurate colorimetric assay was established for assessments of catalase activity in biological fluids and tissues. H2O2 dissociation rates are directly proportional to catalase activity, and the principle of the present assay is based on reactions of ammonium metavanadate with H2O2 under acidic conditions. The resulting reducti...
Article
Full-text available
ABSTRACT Objective: 4-methoxy-N, N-dimethyl-6-(phenylthio) pyrimidin-2-amine, 4-(benzo[d]thiazol-2-ylthio)-6-methoxy-N,N-dimethylpyrimidin-2-amine, 4-(4-(1,2,3-selenadiazol-4-yl)phenoxy)-6-methoxy-N,N-dimethylpyrimidin- 2-amine and 4-(4-(1,2,3-thiadiazol-4-yl)phenoxy)-6-methoxy-N,N-dimethylpyri- midin-2-amine have been synthesized. Method: The pr...
Article
Full-text available
Keeping clean environment is vital matter for human health and other organism. In last decade, the usage of living systems to treat pollution get a real attention. Bioemediation is a way to treat wastewater using enzymes. This study was aimed to introduce a simple and inexpensive method for efficient removal of chlorine substituted phenolic compoun...
Article
Full-text available
Background: The following study presents a colorimetric method for the assessment of serum catalase activity which yields precise, accurate, reproducible results and is simplified so that clinical pathology laboratories may achieve this determination without the need for special techniques. Methods: In this method, dichromate in acetic acid is redu...
Article
Full-text available
Here we provide raw and processed data and methods for the estimation of catalase activities. The method for presenting a simple and accurate colorimetric assay for catalase activities is described. This method is based on the reaction of undecomposed hydrogen peroxide with ammonium molybdate to produce a yellowish color, which has a maximum absorb...
Article
Diabetes is a disease that described by worse glycemic control for which risk of the type IIDMprogresses with age. Anincrease in blood glucose levels causes elevated oxidative stress, which promotes the advancement of diabetes-associated complications.The aim of this research was to clarify the effects of the common oral antidiabetic drugs (glibenc...
Article
Full-text available
Here we provide raw and processed data and methods for the estimation of catalase activities. The method for presenting a simple and accurate colorimetric assay for catalase activities is described. This method is based on the reaction of undecomposed hydrogen peroxide with ammonium molybdate to produce a yel-lowish color, which has a maximum absor...
Article
Full-text available
Obesity is a multifaceted disorder stemming from an imbalance in the homeostasis of energy, which leads to an accumulation of excess energy as fat. It has become of in-creasing concern in the Iraqi population since 2003, when obesity was identified as a significant factor in relation to several diseases. The objective of this study is to inves-tiga...
Article
Full-text available
Background: Oxidative stress and decreased antioxidant levels have been projected as potential factors involved in the pathophysiology of diverse male infertility types, including asthenospermia. The present study was conducted to examine the effect of zinc supplementation on the quantitative and qualitative characteristics of semen along with oxid...
Article
Full-text available
A Fluorometric assay for serum sulfhydryl oxidase activity that could be used as a backup to current tests in the differential diagnosis of spermatozoa disorders is described. The assay is based on the H 2 O 2-dependent oxidation of dichlorofluorescein catalyzed by peroxidase mimetic (a mixture of hematin (HT) and hemoglobin (HB)). The method is se...
Article
Full-text available
Low concentrations of nitric oxide (NO) are necessary for the biology and physiology of spermatozoa, but high levels of NO are toxic and have negative effects on sperm functions. Although several studies have considered the relationship between infertility and semen NO concentrations, no study on the effects of asthenospermia treatments such as ora...
Article
Background: Different urogenital disease and damage factors, such as varicocele, inflammation, and smoking, are well known to influence the balance between radical production and antioxidant defense. The objective of the present study was to select an efficient Non-steriod Anti- inflammatory Drugs (NSAIDs) concentrations of sperm activation in vitr...
Article
Full-text available
A Fluorometric assay for semen xanthine oxidase activity that could be used as a back-up to current tests in the differential diagnosis of spermatozoa disorders is described. The assay is based on the H2O2- dependent oxidation of thiamine catalyzed by peroxidase mimetic (a mixture of hematin (HT) and hemoglobin (HB)). The method is sensitive, preci...
Article
Abstract: Oxidative stress and impaired antiperoxidant levels have been proposed as a potential factors involved in the pathophysiology of diverse male infertility types, including asthenospermia. The present study was conducted to study the effect of zinc supplementation on the quantitative and qualitative characteristics of semen along with an...
Article
ABSTRACT Background There are several causes leading to male infertility, like oxidative stress, and nutritional deficiency of trace elements i.e., selenium and zinc. The Zinc is the following only to iron as the most abundant element in the body. Although, Zinc is found in red meat, white meat, fish, and milk; the World Health Organization (WHO) a...
Conference Paper
ABSTRACT Background The generation of reactive oxygen species (ROS) in the male reproductive tract has become an actual concern because of their probable noxious effects, at high levels, on physical properties of sperm quality. ROS are extremely reactive oxidizing agents, which a member of the class of free radicals. It was found that uric acid can...
Article
Full-text available
ABSTRACT Background The generation of reactive oxygen species (ROS) in the male reproductive tract has become an actual concern because of their probable noxious effects, at high levels, on physical properties of sperm quality. ROS are extremely reactive oxidizing agents, which a member of the class of free radicals. It was found that uric acid can...
Article
ABSTRACT In the present study, a sensitive modification of the colorimetric assay for catalase activity is reported. This assay is based on the reaction of undecomposed hydrogen peroxide with ammonium molybdate to give a yellowish color, whichhas maximum absorbance at 354 nm. Catalase-like activity is calculated from a comparison with the activ...
Article
Full-text available
Semen samples were obtained from 60 fertile (age 31.6 ± 3.3 years) and 60 subfertile (age 32.5 ± 3.23 years) men with asthenozoospermia between July 2011 to July 2012, from couples who had consulted the infertility clinic of Babil Hospital of Maternity (Hilla city/ IRAQ). The subfertile group was treated with zinc sulfate, with every participant...
Article
In the present study, a sensitive modification of the colorimetric assay for catalase activity is reported. This assay is based on the reaction of undecomposed hydrogen peroxide with ammonium molybdate to give a yellowish color, whichhas maximum absorbance at 354 nm. Catalase-like activity is calculated from a comparison with the activity of a c...
Article
ABSTRACT Semen samples were obtained from 37 fertile and 37 subfertile men with asthenozoospermia between July 2011 to April 2012, from couples who consulted the infertility clinic of the Babil hospital of maternity (Hilla city/ IRAQ). The subfertile group consist of the patients which treated with zinc sulfate, every participant took two capsul...
Article
Full-text available
Zinc in human seminal plasma is divided into three types of ligands which are high (HMW), intermediate (IMW), and low molecular weight ligands (LMW). The present study was aimed to study the effect of Zn supplementation on the quantitative and qualitative characteristics of semen along with Zinc Binding Protein levels in the seminal plasma in asthe...
Article
The objective of this study was to develop a relatively simple test to evaluate the stability of the membranes of human spermatozoa. This method depend on measuring the amount of zinc which liberated after exposed to stress resulting from specific concentrations of mercury for a period of time. Membrane that resists or prevents liberation of zinc...
Article
Abstract A simple method for assaying 5'-Nucleotidase activity in biological fluids (serum, seminal plasma, spermatozoa) has been developed. This method is based on the measurement of liberated phosphate by the reaction of phosphate with molybdatein an acidic medium to produce phosphomolybdate complex, and reduce this complex to unstable molybdenu...
Data
Background: Obesity is an important risk factor for the development of different type of degenerative diseases. Objective: Investigate the relationship between oxidative stress and obesity. Methods: 30 Obese (BMI 34-42 kg/m 2) subjects and 20 non-obese (BMI 23-24 kg/m 2)subjects were subject to the present study. Conjugated diene lipid hydroperoxid...
Article
Full-text available
Background: Obesity is an important risk factor for the development of different type of degenerative diseases. Objective: Investigate the relationship between oxidative stress and obesity. Methods: 30 Obese (BMI 34-42 kg/m2) subjects and 20 non-obese (BMI 23-24 kg/m2)subjects were subject to the present study. Conjugated diene lipid hydroperoxides...
Article
bjective:To determine the level of Superoxide dismutase (SOD), Zinc and Copper in the seminal and spermatozoal of patients with asthenospermia. Setting: All semen samples were collected in the infertility unit of the laboratory (in Amel clinic for detection and treatment of infertility/Iraq. Hilla city) after a 3-5 day period of sexual abstinence,...
Article
Full-text available
The concentration of antioxidant such as glutathione, high density lipoprotein (HDL) were measured to assay the oxidative stress associated with Diabetes millitus(DM) compared with healthy controls, Glutathione was found to be reduced when compared with those of healthy controls, this depletion in antioxidant concentration may be due to their prote...

Questions

Questions (2)
Question
I am currently working on measuring the activity of xanthine oxidase enzyme in the liver tissues of some laboratory animals using a laboratory kit manufactured by Merck (Xanthine Oxidase Activity Assay Kit MAK078-1KT). I faced the problem of interference between the xanthine oxidase activity and the catalase enzyme activity. The xanthine oxidase enzyme produces hydrogen peroxide, while the catalase enzyme dissociates the hydrogen peroxide resulting from the enzymatic reaction of xanthine oxidase, thus reducing the accuracy of the results. My question; is what is the appropriate inhibitor that I can use to inhibit catalase enzyme and remove this interference?
Question
In biochemistry, Michaelis–Menten kinetics is one of the best-known models of enzyme kinetics. It is named after German biochemistLeonor Michaelis and Canadian physician Maud Menten. The model takes the form of an equation describing the rate of enzymatic reactions, by relating reaction rate to , the concentration of a substrate S. Its formula is given by
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Projects

Projects (5)
Archived project
Studying genes polymorphism
Archived project
Diabetic Nephropathy