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Mahipal Shekhawat

Mahipal Shekhawat
Kanchi Mamunivar Government Institute for Postgraduate Studies and Research · Botany

Doctor of Philosophy
Plant Structural Plasticity and Changes in Behavioral Patterns. In vitro Plant Biology and Production of Sec Metabolites

About

197
Publications
53,812
Reads
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1,195
Citations
Introduction
Dr. Mahipal S. Shekhawat established 'Biotech Research Unit' @ KMGIPSR, Puducherry. Current projects: Pilot-scale micropropagation of RET, AYUSH & Horticultural crops, Transgenics, Adventitious roots, Sec. metabolites synthesis, Microscopy, Plant structural & behavioural responses, Biosynthesis of NPs and its application on crop improvement, Application of cross-stress in vitro to alleviate morpho-physiological disorder, Histochemical markers, Somatic embryogenesis & Synseeds production.
Additional affiliations
November 2003 - present
K M Government Institute for Postgraduate Studies and Researach
Position
  • Professor (Associate)
Description
  • Presently working on in vitro propagation of rare, endangered and threatened medicinal plants which are endemic to India. Production of bioactive compounds using cell suspension and adventitious roots cultures. Micro-morpho-anatomical analysis
Education
July 1997 - June 2000
Jai Narain Vyas University
Field of study
  • Plant Biotechnology

Publications

Publications (197)
Article
Vanilla planifolia – a natural source of vanillin is the world's highest-valued spice crop with endangered status and the only hand-pollinated crop globally. The present study evaluates the effect of exogenous silver nitrate (AgNO3) on effective direct and indirect somatic embryogenesis in V. planifolia for the first time. The direct and indirect s...
Article
The present study was intended to develop an effective micropropagation system of the economically important ‘Grand Nain’ variety of Musa acuminata Colla (AAA) having high structural fidelity and significant potential to multiply shoots under in vitro conditions using the non-conventional cytokinin 6-benzoyladenine (BOA, C12H9N5O). The young sword...
Article
Full-text available
Global agricultural demand and the impact of fluctuating climatic conditions including global warming have catastrophically limited crop productivity and immensely outstretched the market value of agricultural products leading to acute inflation. The effect of desiccation or drought stress in plants is manifested at three levels viz. morphological,...
Article
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Chitosan is a biopolymer with various favorable properties (biotic/abiotic stress mitiga-tion, qualitative improvement, bio-fertilizer, bio-stimulant and postharvest management) to meet multiple agricultural objectives. Grapevine is an important crop and has an enormous impact on the world's economy due to its derived products, notably the differen...
Chapter
The generation and dumping of electronic (e)-waste are one of the greatest global crises that developing countries are facing. e-waste largely consists of electrical and electronic tools and is considered a source of different types of heavy and precious metals and persistent organic pollutants (POPs) which are reported to cause severe health and e...
Article
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Gloriosa superba L., commonly known as “gloriosa lily,” “glory lily,” and “tiger claw,” is a perennial climber in the Liliaceae family. This plant is used in African and Southeast Asian cultures as an ayurvedic medicinal herb to treat various health conditions. Its main bioactive component is colchicine, which is responsible for medicinal efficacie...
Article
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Lippia javanica is a multipurpose medicinal plant of the Verbenaceae family, which is commonly known for its volatile and essential oil compounds with numerous pharmaceutical applications. Due to medicinal importance, the plant was overexploited, which has been resulted in the rare availability of plant material. Through this study, an efficient an...
Article
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Rauvolfia serpentina (L). Benth. ex Kurz. (Apocynaceae), commonly known as Sarpagandha or Indian snakeroot, has long been used in the traditional treatment of snakebites, hypertension, and mental illness. The plant is known to produce an array of indole alkaloids such as reserpine, ajmaline, amalicine, etc. which show immense pharmacological and bi...
Article
Crinum malabaricum Lekhak & Yadav is a recently discovered and critically endangered aquatic bulbous plant of the family Amaryllidaceae. It gained attention as a wild source of acetylcholinesterase inhibiting alkaloid ‘galanthamine’ to treat Alzheimer’s and Parkinson’s disease. The bulbs of this plant contain the highest amount of galanthamine amon...
Article
A reliable and stable Agrobacterium-mediated genetic transformation system for Artemisia pallens has been developed using cell suspension cultures derived from cotyledon explants. Cotyledon, attached cotyledon, and compound leaves were found to be suitable for the induction of callus among five different types of explants tested. The yellow friable...
Chapter
Stem cuttings-based vegetative propagation is considered to be genetically clonal in nature; but due to lower rooting abilities in most of the plant species, it has become much difficult to propagate the commercial/important plants through stem cuttings. Because of this limitation; in vitro micropropagation of medicinal and commercial plants has ga...
Article
Full-text available
Velamentous roots of orchids are desiccation resistant and help in anchorage and absorption of water and nutrients. The specialized multi-layered, spongy epidermis ‘velamen radicum’ is meant for epiphytic adaptation in economically and ecologically significant ‘grey orchid’ Vanda tessellata. The development of velamen tissue during various stages o...
Chapter
Plants hold the ability to produce wide types of bioactive secondary metabolites. Having emerged in the pregenomic era, increasingly more biosynthetic genes are being discovered in plants, leading to the discovery of new types of bioactive secondary metabolites. Utilisation of classical techniques is limited that hampers the discovery of pharmacolo...
Article
Full-text available
An in vitro regeneration method with functional morpho-structural and physiological traits has been optimized for Aloe vera L. in the present study. Among the various types and treatments of cytokinins [6-benzylaminopurine, BAP; 6-(3-hydroxybenzylamino) purine, mT (meta-topolin), and 1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea, TDZ] tested for shoot in...
Article
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Background: COPD (chronic obstructive pulmonary disease) is a serious health problem worldwide. Present treatments are insufficient and have severe side effects. There is a critical shortage of possible alternative treatments. Medicinal herbs are the most traditional and widely used therapy for treating a wide range of human illnesses around the wo...
Article
Piper betle L. (synonym: Piper betel Blanco), or betel vine, an economically and medicinally important cash crop, belongs to the family Piperaceae, often known as the green gold. The plant can be found all over the world and is cultivated primarily in South East Asian countries for its beautiful glossy heart-shaped leaves, which are chewed or consu...
Article
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Medicinal plants are important for improving human health and represent an essential pool for the identification of novel pharmacological leads. Plant-derived biomolecules have historically proven their value as a source of therapeutic drugs and hold an important potential for the identification and characterization of novel drug leads. Many differ...
Article
Piper betleL. (synonym: Piper betel Blanco), or betel vine, an economically and medicinally important cash crop, belongs to the family Piperaceae, often known as the green gold. The plant can be found all over the world and is cultivatedprimarily in South East Asian countries for its beautiful glossy heart-shaped leaves, which are chewed or consume...
Article
Full-text available
Metastasis or the progression of malignancy poses a major challenge in cancer therapy and is the principal reason for increased mortality. The epithelial-Mesenchymal transition (EMT) of the Basement Membrane (BM) allows cells of epithelial phenotype to transform into a mesenchymal-like (quasi-mesenchymal) phenotype and metastasize via the lymphovas...
Article
In vitro propagation is extremely important for the rapid multiplication of a wide range of orchids. This study aimed to investigate the effects of polyethylene glycol (PEG) on morpho-anatomical improvements and ex vitro development of Spathoglottis plicata, a terrestrial orchid. Various concentrations of PEG (100-500 mg L⁻¹) were incorporated with...
Chapter
Secondary metabolites are synthesized by plants when they are developing or responding to biotic and abiotic environmental stimuli, such as pathogen attack, light, stress, etc., that are used for pharmaceutical production or as nutritional ingredients. Research on secondary metabolites has received particular attention due to their benefits to heal...
Article
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Bacopa monnieri (L.) Wettst. or water hyssop commonly known as “Brahmi” is a small, creeping, succulent herb from the Plantaginaceae family. It is popularly employed in Ayurvedic medicine as a nerve tonic to improve memory and cognition. Of late, this plant has been reported extensively for its pharmacologically active phyto-constituents. The main...
Article
Selenium (Se) is found in plants both in inorganic and organic forms as selenoamino acids and methylated complexes. Se influences growth and physiological parameters, viz. root and shoot growth, starch accumulation, water status regulation, respiration, germination promotion, nitrogen assimilation and delaying senescence in plants. Se deficiency ca...
Article
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Key message The anatomical and histochemical localization at subsequent stages of somatic embryogenesis in Santalum album revealed the direct development of somatic embryos from internode explants. AbstractSantalum album L. (Santalaceae) is a vulnerable tree species due to overexploitation for commercial, pharmaceutical and cosmetic industries, whi...
Article
Full-text available
The current investigation revealed a highly proficient and reproducible method for comprehensive in vitro regeneration and structural differentiation of bulblets in Crinum malabaricum Lekhak & Yadav using meta-topolin [mT; 6-(3-hydroxybenzylamino) purine] [6-benzylaminopurine] for the first time. Cultures were established from twin scales explants...
Article
Full-text available
Polyamines (PAs) are ubiquitous low-molecular-weight, aliphatic compounds with wide as well as complex application in fundamental areas of plant growth and development. PAs are mediator of basic metabolism of organisms which include cell division and differentiation, biotic and abiotic stress tolerance, reversal of oxidative damage, stabilization o...
Chapter
Abstract Herbal medicines, or phytochemicals can treat various neurological disorders, and is actually preferred over synthetic drugs due to their lower cost, negligible side effects, easy availability and therapeutic efficiency. This article lists a total of 13 plant extracts, 27 plant derived natural compounds and 3 herbal formulations that were...
Conference Paper
Full-text available
Best Oral Presentation Award ICEACBS 2022
Article
Crinum malabaricum (Family: Amaryllidaceae) is a critically endangered aquatic medicinal plant endemic to India. This species is a promising natural source of bioactive compounds including galanthamine (GAL), an anti-Alzheimer drug. In vitro regeneration in the Amaryllidaceae is often challenging. This study assessed the use of meta-Topolin (mT) on...
Article
The present investigation describes the efficacy of aromatic and synthetic cytokinins, which influence the direct organogenesis, biochemical responses, and ex vitro and in vivo performance of the in vitro propagated plantlets of Dioscorea pentaphylla L., an underrated famine tuber crop. The axillary shoot bud induction was achieved on the Murashige...
Article
Full-text available
Piperine and piperidine are the two major alkaloids extracted from black pepper (Piper nigrum); piperidine is a heterocyclic moiety that has the molecular formula (CH 2) 5 NH. Over the years, many therapeutic properties including anticancer potential of these two compounds have been observed. Piperine has therapeutic potential against cancers such...
Article
Dioscorea deltoidea Wall. ex Griseb. (family Dioscoreaceae) has been traditionally used as a remedy for various human ailments. In modern medicine, it is used as an efficacious remedy against diabetes and cardiovascular diseases. Dioscorea deltoidea tubers are known to be a rich reservoir of diosgenin. A consistent and reproducible protocol is need...
Chapter
Phytohormones play significant roles in plants for its growth and development. These phytohormones have been identified to regulate stress tolerance in plants. Gibberellic acid is one such phytohormones which carries out several important roles, GA crosstalk with many other phytohormones like ethylene, jasmonic acid, brassinosteroids, strigolactone...
Article
Dioscin has gained immense popularity as a natural, bioactive steroid saponin, which offers numerous medical benefits. The growing global incidence of disease-associated morbidity and mortality continues to compromise human health, facilitating an increasingly urgent need for nontoxic, noninvasive, and efficient treatment alternatives. Natural comp...
Article
Full-text available
Monoamine oxidase (MAO) is capable of catalysing the oxidative deamination of amines and neurotransmitters. MAO plays a pivotal role in maintaining neurotransmitters linked to neurological disorders viz. Alzheimer's disease (AD), Parkinson's disease (PD) etc. Therefore, inhibition of MAO can be implicated to the cure of such diseases. Synthetic MAO...
Article
Full-text available
Santalum album L. (Indian sandalwood) is an economically important but vulnerable tropical tree species. Cultures were established via direct shoot regeneration from axillary buds on Murashige and Skoog (MS) medium supplemented with 2.5 mg L⁻¹ 6-benzylaminopurine (BAP). The shoots were multiplied using MS medium containing 1.0 mg L⁻¹ BAP and 0.5 mg...
Article
An incredible array of natural products are produced by plants that serve several ecological functions, including protecting them from herbivores and microbes, attracting pollinators, and dispersing seeds. In addition to their obvious medical applications, natural products serve as flavouring agents, fragrances and many other uses by humans. With t...
Article
The present report elucidates the foliar micro-morphological adaptation mechanism of micropropagated plantlets of Aerva lanata. The in vitro induced anomalies in the leaves were repaired through gradual hardening and acclimatization processes in a greenhouse. The epidermal tissues of in vitro grown leaves significantly differed from the field trans...
Article
Dalbergia latifolia Roxb. (Fabaceae), famed for its wood, is a vulnerable plant species according to the International Union for Conservation of Nature and Natural Resources (IUCN) Red List as a result of its over-utilisation. This mini-review, which provides a synthesis of the micropropagation of this woody plant species, can be useful for the est...
Article
Full-text available
Oxystelma esculentum (L. f.) Sm. (Apocynaceae) is a perennial medicinal climber, enormously explored in traditional and modern systems of medicines. Due to over-harvesting from the wild, this species is categorized as ‘rare’. Hence, this study was aimed to develop an in vitro regeneration system for O. esculentum as a conservation measure. Nodal ex...
Article
An improved in vitro propagation system has been developed for Santalum album L. using meta-Topolin for the first time. Direct organogenesis (100 %) was achieved by induction of axillary buds and a maximum of 8.2 shoots with 4.0 cm length was developed from nodal explants on Murashige and Skoog’s (MS) medium containing 1.0 mg L⁻¹ meta-Topolin (mT)....
Article
Plantlets of Hemidesmus indicus (L.) R. Br. (Indian sarasaparilla) were developed in vitro in two different types of culture vessels and closure types-(1) culture bottles with polypropylene screw-caps and (2) conical flasks with cotton plugs both containing Murashige and Skoog medium with 1.0 mg L −1 6-benzylaminopurine, 0.5 mg L −1 kinetin, and 0....
Article
An effective plant regeneration protocol via a liquid culture system has been ascertained in Spathoglottis plicata Blume (Orchidaceae), an important orchid of the horticulture industry. The seeds were germinated asymbiotically on the agar-gelled Murashige and Skoog’s (MS) medium supplemented with 1.0 mg L− 1 6-benzylaminopurine (BAP) and about 93.0...
Article
Mucuna pruriens L. (DC.), is a valuable, economically significant medicinal crop, known worldwide for producing the anti-Parkinson's drug Levodopa (L-Dopa). Itching property of the plant poses certain constrains in its conventional breeding, but, in vitro cell culture systems offer advantage in which controlled conditions can be used for the produc...
Article
The well-known medicinal plant Centella asiatica (L.) Urban is an Ayurvedic and traditional Chinese medicine used in the treatment of different health problems and as an edible vegetable in a regular diet. Ease of availability in the wide range of environmental conditions plus low-cost cultivation process has made the plant popular in ethno-medicin...
Chapter
Dendrocalamus strictus (Roxb.) Nees (family: Poaceae) is an economically important bamboo species explored worldwide for its wider adaptability. In vitro propagation is an alternate platform and has astonishing benefits in large-scale production of disease-free clones of the important plant species. Despite several efforts implicated to improve the...
Article
Full-text available
An advanced micropropagation protocol has been developed for the global spice crop Vanilla planifolia using meta-topolin [mT, 6-(3-hydroxybenzylamino) purine] for the first time. Among the two types of cytokinins [mT and BAP (6-benzylaminopurine)] experimented, healthy shoot development and growth in terms of shoot numbers (5.0 ± 0.20/explant) with...
Article
Full-text available
Spathoglottis plicata Blume is a vulnerable orchid species in various parts of the world, and the conventional propagation provides limited success to its cultivation and conservation. Therefore, present study deals with the direct induction of somatic embryos (SEs) from the leaf explants of S. plicata. Murashige and Skoog’s (MS) medium fortified w...
Article
Origanum majorana (L.) (family Lamiaceae) is a multipurpose medicinal plant with high demand across the pharmaceutical, cosmetic, food, beverage, and perfume industries that has raised concern for its mass propagation through in vitro culture techniques. The present report describes an effective in vitro propagation system for an aromatic medicinal...
Article
Micropropagation techniques allow producing large numbers of clones of genetically identical plants. However, there is evidence of disorders in internal structures due to sophisticated in vitro conditions. Such variations are responsible for the mortality of plantlets in the field and cause huge loss to the tissue culture industry. Anatomical evalu...
Preprint
Full-text available
A reliable and stable Agrobacterium -mediated genetic transformation system has been developed using cell suspension cultures derived from Artemisia pallens cotyledon explants. Cotyledon, attached cotyledon, and compound leaf were found to be suitable for the induction of callus among five different types of explants tested. Yellow friable callus d...
Article
Vanda tessellata (Roxb.) Hook. ex G. Don. (Orchidaceae) is an epiphytic orchid species, commonly known as grey orchid, and explored enormously for its significant horticultural, medicinal, phytochemical and pharmacological properties. The present study describes an in vitro culture approach via asymbiotic seed germination assisted by phytohormones...
Article
Full-text available
Ferocactus peninsulae (A.A.Weber) Britton & Rose (Barrel cactus) belongs to the family Cactaceae, has a great potential for commercial exploitation by the horticulture industry and landscape projects. It is difficult to propagate vegetatively because of slow and columnar growth; hence, its natural population is decreasing. Therefore, it is indeed d...
Presentation
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Going to deliver a lecture and having a virtual workshop on Foldscope: its Assembly and Applications
Article
Ferocactus peninsulae (A.A.Weber) Britton & Rose (Cactaceae) has great potential for commercial exploitation by the horticulture industry. It is difficult to propagate vegetatively, and we investigated novel propagation methods. The objectives were to optimize the organic supplements in nutrient medium for seed germination, synthetic seed (synseeds...
Presentation
Full-text available
As a resource person delivered a talk on Recent Advances in Plant Cell and Tissue Culture to the audiances of different parts of India.
Article
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Scaevola taccada (Gaertn.) Roxb. is a hemi-sclerophyllous littoral shrub of the family Goodeniaceae. It is a salt-tolerant plant and used in soil reclamation and coastal landscaping programs to control beach erosion. The present article reports a reproducible in vitro regeneration system using meta-topolin (mT) through liquid medium employing nodal...
Article
The present communication reports, induction of adventitious roots (AR) from the in vitro raised leaves of Morinda coreia as affected by auxins and their varying concentrations. The roots of this plant possess anthraquinone, a preferable natural dye, explored well in pharmaceuticals. The AR from the leaves were induced under light and dark conditio...
Article
Full-text available
Vanda tessellata (Roxb.) Hook. ex G.Don (grey orchid) is a horticultural and medicinally potent, and conservation prioritized orchid species. The present study investigates asymbiotic seed germination, callus induction, somatic embryogenesis, encapsulation of somatic embryos (SEs), and retrieval of plantlets of V. tessellata from the artificial see...
Article
Ormocarpum sennoides (bone-knit) is a vulnerable medicinal plant species belongs to the family Leguminosae. The aim of the present study was to evaluate the influence of plant growth regulators (PGRs), additives (ascorbic acid, citric acid, adenine sulfate, and L-arginine), gelling agents (agar and phytagel™) and type of culture vessels on the quan...