Mahinda Senevirathne

Sabaragamuwa University of Sri Lanka | SUSL · Department of Food Science and Technology

The post-harvest loss of rice during storage due to insect infestation, particularly by rice weevil ( Sitophilus oryzae ), and the utilization of synthetic insecticides for pest management are two major concerns in the current world. Therefore, this study aimed to evaluate the potential of clove ( Syzygium aromaticum ) part formulations as alternat...

Penaeus monodon is the mostly exported shrimp in Sri Lanka. This study was conducted to evaluate the effect of gamma irradiation on the microbial, physical and chemical parameters of postharvest P. monodon. Samples from 10 farms were irradiated at 1 kGy, 2 kGy, 3 kGy, 5 kGy and 10 kGy. Total plate count (TPC), yeast and mold count (Y&M), coliform c...

Green leaves are the primary sites of photosynthesis and a lot of living beings depend on that. Green leaves rich with bioactive compounds like Phenolic compounds, vitamins, flavonoids and medicinal properties like antioxidant, antimicrobial, anticancer, antidiabetic activities. Therefore some green leaves are used to treat many diseases and condit...

The marine environment has a tremendous biodiversity and is the source of a plethora of biologically active molecules with great potential in pharmaceutical and medicinal applications. Consequently a number of functional materials have been identified including, fish muscle proteins, peptides, collagen and gelatin, fish oil, fish bone. Biologically...

In this study, we fermented sea tangle (FST) using Lactobacillus brevis BJ20, and its protective effect against the ethanol‐induced hepatotoxicity in Sprague‐Dawley rats was evaluated. Administration of ethanol to rats for 3 weeks induced liver damage with significant increase in hepatic malondialdehyde level and decrease in glutathione and antioxi...

Great amount of marine fish species have been identified with potential nutraceutical and medicinal values. Consequently, a number of bioactive compounds have been identified including fish muscle proteins, peptides, collagen and gelatin, fish oil, fish bone. Bioactive peptides derived from various fish muscle proteins have shown various biological...

Large amount of underutilized by-products are generated from the seafood processing plants annually. Consequently, researches have been initiated to investigate those discarded materials and have identified a number of bioactive compounds including bioactive peptides, collagen and gelatin, oligosaccharides, fatty acids, enzymes, calcium, water-solu...

In this study, the antibacterial effect was evaluated to determine the benefits of high speed drying (HSD) and far-infrared radiation drying (FIR) compared to the freeze drying (FD) method. Citrus press-cakes (CPCs) are released as a by-product in the citrus processing industry. Previous studies have shown that the HSD and FIR drying methods are mu...

In this study, eight kinds of phenolic acid conjugated chitooligosaccharides (PA-c-COSs) with different substitution groups, including p-hydroxyl {hydroxybenzoic acid-c-COS (HBA-c-COS), p-coumaric acid-c-COS (PCA-c-COS)}, 3,4-dihydroxyl {protocatechuic acid-c-COS (PTA-c-COS), caffeic acid-c-COS (CFA-c-COS)}, 3-methoxyl-4-hydroxyl {vanillic acid-c-C...

Antioxidant, anti-Alzheimer and anti-inflammatory activities of enzymatic hydrolysates from Laminaria japonica were evaluated in this study. Four proteases (Protamex, Neutrase, Flavourzyme and Alcalase) and seven carbohydrases (Viscozyme, Celluclast, Dextrozyme, Amyloglucosidase [AMG], Promozyme, Termamyl and Maltogenase) were used in hydrolysis. A...

Gallic acid-grafted-chitosans (GA-g-chitosans) were prepared according to our previous method, and the in vitro protective effect of the various GA-g-chitosans on tert-butyl hydroperoxide (t-BHP)-induced oxidative stress was investigated in Chang liver cells. Pretreatment of the GA-g-chitosans decreased cell damage induced by t-BHP in a dose-depend...

Asthma is a variable disease and various factors are affected to increase the asthmatic symptoms and level of asthma control. It is believed that the cause for this disease is a combination of genetic and environmental factors. Numerous medications are available at present to treat this disease but it has been failed to control number of incidences...

Sea-food processing wastes and underutilized species of fish are a potential source of functional and bioactive compounds. A large number of bioactive substances can be produced through enzyme-mediated hydrolysis. Suitable enzymes and the appropriate bioreactor system are needed to incubate the waste materials. Membrane separation is a useful techn...

IntroductionMarine algae-derived functional ingredients and their antioxidant effectConclusion References

In the present study, a suitable drying method was developed for citrus press cakes (CPCs), which are produced as a by-product in citrus juice plants, and the protective effect of methanol extract of CPCs prepared by far-infrared radiation (FIR) drying against H(2)O(2)-induced DNA damage was evaluated versus that of freeze-dried CPCs. Methanol extr...

Hepatoprotective effect of chitooligosaccharides (COSs) with different molecular weights was investigated against tert-butylhydroperoxide (t-BHP)-induced damage in Chang liver cells. For that, we evaluated the cell viability, inhibition of production of reactive oxygen species (ROS), lipid peroxidation inhibition, glutathione (GSH) contents and the...

Enzymatic extracts from Porphyra tenera were prepared using 4 proteases (Protamex, Neutrase, Flavourzyme, and Alcalase) and 7 carbohydrases (AMG, Celluclast, Dextrozyme, Maltogenase, Termamyl, Promozyme, and Viscozyme), and biological activities of the enzymatic extracts from P. tenera were determined as antioxidant, anti-acetylcholinestrase (AChE)...

We extracted bioactive materials from Codium fragile by enzymatic hydrolysis using four different proteases (Alcalase, Flavourzyme, Neutrase, and Protamex) and seven different carbohydrases (amyloglucosidase (AMG), Celluclast, Dextrozyme, Maltogenase, Promozyme, Termamyl, and Viscozyme), and evaluated their biological activities such as antioxidant...

Blueberry was enzymatically hydrolyzed using selected commercial food grade carbohydrases (AMG, Celluclast, Termamyl, Ultraflo and Viscozyme) and proteases (Alcalase, Flavourzyme, Kojizyme, Neutrase and Protamex) to obtain water soluble compounds, and their protective effect was investigated against H(2)O(2)-induced damage in Chinese hamster lung f...

In this study, far-infrared radiation (FIR) drying was used to convert wet citrus press-cakes (CPCs) into dried form at different temperatures (40, 50, 60, 70, and 80 °C). The dried samples were evaluated for flavonoid composition by HPLC and antioxidant activities by various in vitro assays. Compared with CPCs prepared by freeze drying (FD), all F...

Sea tangle has long been used as Korean folk remedy to promote material health, and is one of the popular dietary supplement. This study was designed to evaluate the protective effect of fermented sea tangle (FST) against ethanol and carbon tetrachloride (CCl(4))-induced hepatotoxicity in rats. Sprague-Dawley rats were orally treated with FST (25,...

This study was carried out to investigate total polyphenol contents, total flavonoid contents and antioxidative activities of the methanol extracts from dried Citrus sunki and C. unshiu peels. The dried C. sunki peels displayed higher total polyphenol and flavonoid contents for older dried peels. In contrast, the dried C. unshiu peels displayed hig...

In this study, factors affecting anti-inflammatory effect of chitooligosaccharides (COSs) in lipopolysaccharides (LPS)-induced RAW264.7 macrophage cells were investigated. The inhibition of NO secretion by COSs revealed that 90-COSs (90% N-deacetylation) significantly inhibited NO secretion than those of 50-COSs (50% N-deacetylation), and 90-HMWCOS...

The aim of this study was to evaluate the antioxidant activities of enzymatic digests from citrus by-products (CBPs) prepared by high speed drying (HSD). HSD needs a short time (60 min) for drying and can be used in a commercial scale. Enzymatic digests were prepared from the CBPs using 6 enzymes such as aminoglucosidase (AMG), celluclast, pectinex...

The citrus by-products (CBP) released from the citrus processing plants may contain high amount of potential bioactive compounds. Hence, this study aims to evaluate the antioxidant activity of CBP prepared using a novel drying technique known as high speed drying (HSD), economically and efficiently. HSD extract showed high amount of polymethoxylate...

This study was conducted to investigate total polyphenolic contents and reactive oxygen species (ROS) scavenging effects of extracts from peels of ten Jeju native citrus fruits according to the harvest from August 2006 to February 2007. Total polyphenolic contents from methanol extracts of citrus peel were the highest in Jigak (Citrus aurantium) an...

Efficiency of a far-infrared radiation (FIR) dryer for drying of citrus by-products (CBPs) was evaluated through their antioxidant activities. The CBPs dried through FIR were enzymatically digested by six carbohydrases (AMG, Celluclast, Pectinase, Termamyl, Ultraflo and Viscozyme) to prepare digests for evaluation of the activities. The total polyp...

The enzymatic extracts from seven species of microalgae (Pediastrum duplex, Dactylococcopsis fascicularis, Halochlorococcum porphyrae, Oltmannsiellopsis unicellularis, Achnanthes longipes, Navicula sp. and Amphora coffeaeformis) collected from three habitats (freshwater, tidal pool, and coastal benthic) at Jeju Island in Korea were investigated for...

Sargassum fulvellum, a marine brown alga, is a popular low priced edible plant in Korean markets. The polysaccharide fraction of the alga was separated and investigated for its radical scavenging activities and the results compared with those of commercial fucoidans (Fucus vesiculosus and Undaria pinnatifida), BHA and -tocopherol. The polysaccharid...

The enzymatic extracts from seven species of micro-algae (Pediastrum duplex, Dactylococcopsis fascicula-ris, Halochlorococcum porphyrae, Oltmannsiellopsis unicellularis, Achnanthes longipes, Navicula sp. and Amphora coffeaeformis) collected from three habi-tats (freshwater, tidal pool, and coastal benthic) at Jeju Island in Korea were investigated...

The antioxidative potential of different fractions (respective organic and aqueous fractions of n-hexane, chloroform and ethyl acetate) of 70% methanol extract of Ecklonia cava(a brown seaweed) was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide anion, hydrogen peroxide, hydroxyl radical, nitric oxide, ferrous ion chelating, reduci...

Enzymatic extracts were prepared from the blueberry (Vaccinium corymbosum L.) collected in Jeju, Korea. Five carbohydrases namely AMG, Celluclast, Termamyl, Ultraflo and Viscozyme, and five proteases namely Alcalase, Flavourzyme, Kojizyme, Neutrase and Protamex were used to prepare the enzymatic extracts. Antioxidant properties of each extracts wer...

The antioxidative potential of different fractions (respective organic and aqueous fractions of n-hexane, chloroform and ethyl acetate) of 70% methanol extract of Ecklonia cava (a brown seaweed) was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide anion, hydrogen peroxide, hydroxyl radical, nitric oxide, ferrous ion chelating, reduc...

The antioxidant potential of a 75\% methanolic extract of highbush blueberry (Vaccinium corymbosum L.) and its different fractions was investigated using different reactive oxygen species (ROS), nitric oxide (NO.), metal chelating and lipid peroxidation assays. Methylene chloride and 75\% methanol fractions showed equally high activities (IC_{50} 0...