Maha Abbas

Maha Abbas
Al Qasim Green University · Biotechnology

Ph.D Molecular virologist/ University Lecturer

About

12
Publications
17,853
Reads
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141
Citations
Introduction
I have worked as a research scientist in the Virology lab in Germany from 2008 until 2013 where I was introduced to a wide range of diagnostic methods. My work afterwards as a lab analyst in one of the major laboratories in Baghdad/ Iraq has also enhanced my skills and lab experience.
Additional affiliations
August 2016 - present
Al Qasim Green University
Position
  • Lecturer
March 2014 - July 2016
Al-Ansega clinical Lab
Position
  • Analyst
October 2008 - February 2013
University of Hohenheim
Position
  • Researcher
Description
  • I was exposed during my daily routine work of diagnosing viruses from clinical samples to a wide range of diagnostic methods.
Education
October 2006 - October 2008
Universität Hohenheim, Institute of envirnmental and animal hygien and animal medicine
Field of study
  • Molecular virology

Publications

Publications (12)
Article
Full-text available
Ferlaviruses are important pathogens in snakes and other reptiles. They cause respiratory and neurological disease in infected animals and can cause severe disease outbreaks. Isolates from this genus can be divided into four genogroups–A, B, and C, as well as a more distantly related sister group, “tortoise”. Sequences from large portions (5.3 kb)...
Research
Full-text available
Respiratory syncytial virus (RSV) is one of the most common respiratory viruses that causes bronchiolitis in infant all over the world. Differentiation of the contiguous genotype A and B of this virus in children less than five years old with influenza like illness (ILI) was carried out by real-time RT-PCR targeting the Fusion (F) and Glycoprotein...
Article
Full-text available
Blood samples were collected from 135 children under 11years of age suspected with fever and sepsis in 2013, obtained from Welfare Teaching Hospital/Medical City/Baghdad. The blood samples were tested for bacteremia using conventional blood culture and polymerase chain reaction (PCR) targeting the 16s rRNA and the Lac Z gene. The results indicated...
Article
Full-text available
Sera from a total of 202 tortoises from six countries and nine species were tested for antibodies against four different reptilian paramyxoviruses (ferlaviruses, ferlaVs) by hemagglutination inhibition (HI) test. The viruses used were a tortoise PMV (tPMV) and three squamatid PMV isolates, each belonging to a different subgroup of ferlaV within the...
Article
Full-text available
Respiratory diseases in boid snakes are common in captivity, but little information is available on their aetiology. This study was carried out to determine the occurrence of lung associated pathogens in boid snakes with and without respiratory signs and/or pneumonia. In total, 80 boid snakes of the families Boidae (n = 30) and Pythonidae (n = 50)...
Article
Full-text available
In May 2011, 22 cloacal swabs and plasma samples were collected from 22 bearded dragons (Pogona vitticeps) from a rescue center in Munich, Germany. All of the lizards swabbed were healthy throughout the observation period and did not show any clinical signs during the course of the study. The swabs were tested for the presence of ferlaviruses (Para...
Article
During the course of a longitudinal survey on the occurrence of viruses in Hungarian exotic reptile collections a dead masked water snake (Homalopsis buccata) was submitted for virologic examination in September 2009. Based on history, gross pathological and histopathological findings paramyxovirus infection was suspected and later confirmed by RT-...
Article
Full-text available
A common agama (Agama agama) with clinical signs of dehydration, anorexia, and an abscess in the oral cavity was presented for medical examination. Bacteriological testing of an oral swab demonstrated the presence of Proteus vulgaris and Morganella morganii (Proteus morganii). A fecal examination showed oocysts of Choleoeimeria sp. and pinworm eggs...
Article
Full-text available
In 2009, 26 clinical samples (organs and oral/cloacal swabs) from a total of 24 corn snakes (Pantherophis guttatus) from a single owner were sent to our laboratory to be tested for the presence of viruses. Paramyxoviruses (PMV), adenoviruses (AdV) and reoviruses were detected by RT-PCR, PCR and virus isolation methods. Three snakes were infected wi...
Article
Full-text available
To determine the role of water fleas in accumulating avian influenza viruses (AIV) from the surrounding water and to estimate their role as a vector of AIV. Water fleas were exposed to H4N6 and H5N1 AIV-contaminated water in a closed system. The potential of water fleas to take up and retain the viruses was estimated by quantitative real-time RT-PC...
Article
A germ carrier technique was adapted for the determination of the persistence of influenza viruses in moist environments. The technique was employed with 3 low pathogenic avian influenza viruses (H4N6, H5N1, and H6N8), one human influenza virus (H1N1), and two model viruses (NDV and ECBO) in lake water at five different temperatures (30, 20, 10, 0,...

Questions

Questions (2)
Question
What would you do if you have dicovered a novel technique that may make a big change in the routine lab work, what would you do to bring it into the reality? participation in a conference, publishing this work, contact some companies? what would you do ? what would be the first step?
Question
I am trying to follow the M&M part as indicated in this link to prepare the working concentration of primers. In the paper, it is written that the working concentration of the primers is supposed to be 100ng/µl. I have already looked and searched the Internet, I found some formulae, but I am not sure that they are accurate.
1ng/ml=3.47 nmole/l
so to convert I did as follows:
1ng/1000µl = 3.47x nmole/1000ml
0.001ng/µl=3.47 x 0.001nmole/ml
0.001ng/µl= 3.47xpmole/1000µl
1ng/µl=3.47pmole
100 ng/µl =347pmole/µl
This cannot be true as it is too high. Do you have any suggestions?

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