Magdalena Karas

Magdalena Karas
Maria Curie-Sklodowska University in Lublin · Department of General Microbiology and Genetics

Assistant Professor

About

17
Publications
2,910
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123
Citations
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November 1996 - present
Maria Curie-Sklodowska University in Lublin

Publications

Publications (17)
Article
Full-text available
Anthropogenic activities generate a high quantity of organic pollutants, which have an impact on human health and cause adverse environmental effects. Monitoring of many hazardous contaminations is subject to legal regulations, but some substances such as therapeutic agents, personal care products, hormones, and derivatives of common organic compou...
Article
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Lipopolysaccharide (LPS) is the major glycolipid and virulence factor of Gram-negative bacteria, including Aeromonas spp. The O-specific polysaccharide (O-PS, O-chain, O-antigen), i.e., the surface-exposed part of LPS, which is a hetero- or homopolysaccharide, determines the serospecificity of bacterial strains. Here, chemical analyses, mass spectr...
Article
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Innate immunity is an evolutionarily ancient form of host defense that serves to limit infection. The invading microorganisms are detected by the innate immune system through germline-encoded PRRs. Different classes of PRRs, including TLRs and cytoplasmic receptors, recognize distinct microbial components known collectively as PAMPs. Ligation of PA...
Article
Full-text available
In our previous report, we had shown that the free-living amoeba Acanthamoeba castellanii influenced the abundance, competiveness, and virulence of Mesorhizobium loti NZP2213, the microsymbiont of agriculturally important plants of the genus Lotus. The molecular basis of this phenomenon; however, had not been explored. In the present study, we demo...
Article
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Reversible phosphorylation is a key mechanism that regulates many cellular processes in prokaryotes and eukaryotes. In prokaryotes, signal transduction includes two-component signaling systems, which involve a membrane sensor histidine kinase and a cognate DNA-binding response regulator. Several recent studies indicate that alternative regulatory p...
Article
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This is the first report describing isolates from root nodules of Ononis arvensis (field restharrow). The aim of this investigation was to describe the diversity, phylogeny, and plant growth promoting features of microsymbionts of O. arvensis, i.e., a legume plant growing in different places of the southern part of Poland. Twenty-nine bacterial iso...
Article
The O-specific polysaccharide (OPS) was isolated from the lipopolysaccharide of Aeromonas hydrophila strain K691 and studied by chemical methods and (1)H and (13)C NMR spectroscopy, including 2D (1)H,(1)H COSY, TOCSY, NOESY, (1)H-detected heteronuclear (1)H,(13)C HSQC, and HMBC experiments. It was found that the O-specific polysaccharide was built...
Article
Full-text available
Rhizobium leguminosarum bv. trifolii is capable of establishing a symbiotic relationship with plants from the genus Trifolium. Previously, a regulatory protein encoded by rosR was identified and characterized in this bacterium. RosR possesses a Cys2-His2-type zinc finger motif and belongs to Ros/MucR family of rhizobial transcriptional regulators....
Article
The outer core oligosaccharide (OS) was isolated from the lipopolysaccharide (LPS) of Rhizobium leguminosarum bv. trifolii strain 24 after Smith degradation and then studied by sugar and methylation analyses along with NMR and mass spectrometry methods. Negative-ion electrospray (ESI-MS) mass spectrum showed two molecular ions at m/z 686.3 and 728....
Article
Full-text available
The importance of protozoa as environmental reservoirs of pathogens is well recognized, while their impact on survival and symbiotic properties of rhizobia has not been explored. The possible survival of free-living rhizobia inside amoebae could influence bacterial abundance in the rhizosphere of legume plants and the nodulation competitiveness of...
Article
Full-text available
Ether lipids were identified among components liberated with HF and nitrous acid deamination from Acanthamoeba rhysodes whole cells and its membrane glycoinositolphospholipids (GIPL). Liberated ether glycerols were converted to various derivatives that served characterization thereof. These included TMS and isopropylidene derivatives, oxidation wit...
Article
Full-text available
The polymer called lipophosphonoglycan (LPG) was isolated from Acanthamoeba castellanii membranes after exhaustive delipidation and butanol extraction. A novel extremely long phytosphingosine was revealed in glycoinositolphosphosphingolipid (GIPSL). All data obtained by gas-liquid chromatography coupled with MS analyses of products liberated during...
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Dialysis patients and patients post-renal transplantation can be predisposed to Legionella infections. The aim of this work was to investigate the prevalence of L. pneumophila serogroups 1-7 (SG 1-7) antibodies in dialysis patients and in patients following renal transplantation, in order to analyse the potential risk factors for infections. Materi...
Article
Full-text available
Short glucan oligomers were found to be covalently linked to the peptidoglycan fragments liberated by partial lysozyme digestion of Mesorhizobium loti HAMBI 1148 murein and among some muropeptide mono and oligomers subsequently subjected to alkaline β-elimination. The mass spectra of the preparation before and after treatment with alkali contained...
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The location and nature of the linkage between peptidoglycan and oligoglucans in the cell wall of Mesorhizobium loti HAMBI 1148 have been defined by the analysis of nitrous acid deamination of peptidoglycan glucosaminyl residues. The MurNH(2)-Glc(n) fraction was obtained after converting deaminoacylated and N-deacetylated muramyl residues in the ce...
Article
Full-text available
summary. The cellular fatty acid composition of Acanthamoeba castellanii, a unicellular bacteriovorous organism, was reinvestigated. Lipids from amoebae grown axenically in proteose peptone-yeast extract-glucose medium were extracted with chloroform–methanol and separated by silicic acid column chromatography into non-polar and polar fractions. The...
Article
The murein sacculus is the essential part of cell wall of almost all eubacteria. It is needed to withstand the cytoplasmic turgor pressure and maintain a specific cell shape. The sacculus of bacteria appears to be a single molecule covering the inner membrane of bacteria. During bacteria growth the sacculus is enlarged continuously without rapture...

Questions

Question (1)
Question
I have extremely long phyo LCBs C24-28 with FA C22-28 so it's impossible to analyse them by GC-MS. Can anyone recommend a method to identify them in a mixture?

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