M. Manokari

• Doctor of Philosophy
• Asst Research Officer at Central Council for Research in Siddha (M/o AYUSH) India

Aechmea bracteata (Sw.) Griseb. is an important bromeliad and is considered for ex situ conservation. Epiphytism and Crassulacean acid metabolism (CAM) make this species resilient to ecological stresses. This study aimed to illustrate the periodic development of epiphytic structural traits in micropropagated plantlets of A. bracteata through in vit...

Hemidesmus indicus (L.) R. Br. [Indian sarasaparilla] is an herbaceous climber used in traditional and modern medicines. This species is overexploited due to increased demand for its roots, resulting in population loss. Hence, there’s a necessity to explore biotechnological processes that aid in producing roots, which is crucial for conserving and...

Ferocactus peninsulae (F.A.C. Weber) Britton & Rose, commonly known as the barrel cactus, is a distinctive and visually striking succulent belonging to the Cactaceae family. It is renowned for its impressive size and robust, barrel-shaped structure. F. peninsulae faces potential threats from habitat destruction and illegal collection. This study in...

Coccoloba uvifera L. (Polygonacaeae), named also seagrape, is an ectomycorrhizal (ECM) Caribbean beach tree, introduced pantropically for stabilizing coastal soils and producing edible fruits. This review covers the pantropical distribution and micropropagation of seagrape as well as genetic diversity, functional traits and use of ECM symbioses in...

Pisonia grandis R. Br. is a tree commonly found in urban coastal and tropical regions, valued for its ornamental beauty and utility in agroforestry and traditional medicine. To facilitate its propagation, particularly through in vitro techniques, this study aimed to refine a propagation system by inducing adventitious buds from node explants of mat...

Plant genetic transformation has emerged as a key platform for both fundamental and translational research. The reporter genes are widely used to visualize gene expression patterns, promoter analysis, localization, and stable transformation studies. Marker genes would be expected to exhibit characteristics such as robustness, chemical affordability...

Background Dendrocalamus strictus (Roxb.) Nees, generally referred to as ‘Male bamboo,’ is a globally prevalent and highly significant species of bamboo. It is a versatile species and possesses notable industrial significance. However, despite its numerous applications, the production of this plant is insufficient to fulfill the worldwide demand. T...

The present study highlights the effect of silver nitrate (AgNO3) in the promotion of in vitro regeneration and morpho-structural growth and developments in Oxystelma esculentum (L.f.) Sm. The incorporation of AgNO3 into the Murashige and Skoog (MS) medium resulted in a favorable enhancement of shoot proliferation. Among the different concentration...

Nanosilicon positively affects the growth and development of plants. Investigations were carried out to analyse the effect of applied silicon nanoparticles (SiNPs) on the multiplication and rooting of the shoots...

Santalum album L. (Santalaceae) is an endangered tree species with high economic potential. To improve its cultivation, the role of strigolactone on the somatic embryogenesis of Santalum leaves was analyzed. Leaves were cultivated in vitro using GR24 (a synthetic analogue of strigolactone) at various concentrations (from 0.1 to 1.0 mg L−1) and meta...

Silicon nanoparticles (SiNPs) in Murashige and Skoog (MS) medium improved micropropagation of Hemidesmus indicus (L.) R. Br. ex Schult. (Apocynaceae), a valuable and threatened medicinal plant. Nodal explant-derived shoots amplified (130 shoots/inoculum/culture vessel) on MS medium + 1.0 mg L⁻¹ SiNPs and 1.0 mg L⁻¹ 6-benzylaminopurine (BAP) + 0.5 m...

Experimental studies have been conducted to evaluate the induction of mini-tubers in vitro from shoots of Dioscorea pentaphylla L. as affected by applications of Silicon nanoparticles (SiNPs). Amorphous nano-silica is a low-cost material and is considered biologically safe because of its biodegradability. A maximum number of mini-tubers (1.9) per s...

Thepresentstudyaimedtoanalyzetheeffectofsiliconnanoparticles(SiNPs)ontheinvitroproliferationofshoots,biomassproduction,foliarmicro-structuralfeatures,androot-inducingabilityinVitextrifoliaL.Budbreakingwasachievedfromaxillarymeristemson2.5mg/Lof 6 benzylaminopurine(BAP)inMurashigeandSkoog’s(MS)medium.Theshootswereproliferated(18.0§0.39shoots)on0.75m...

Two forms of the genus Passiflora, belonging to the Passifloraceae family, are commonly called yellow and purple passion. These perennial woody climbers are found in the cooler regions at higher altitudes and in lowlands of tropical areas. The presence of alkaloids, terpenes, stilbenes, flavonoids, glycosides, carotenoids, etc. in different parts o...

Cymbopogon citratus (DC.) Stapf (lemongrass or citronella) is a herb of therapeutic potential highly sought by various industries due to the presence of essential oil. In vitro propagation of commercial crops is highly desirable; it is an alternative approach for the production of quality planting materials. But the plantlets encounter certain morp...

Experimental studies were conducted on the effects of seismic stress on (a) in vitro shoot proliferation and (b) induction of stress tolerance in Dioscorea pentaphylla L. cultivated in vitro. The morphometric traits in plantlets developed under in vitro seismic stress set at 60 rpm for 8.0 min twice a day for 4 weeks promoted the number of shoots p...

Systematic studies on incorporation of Silicon nanoparticles (SiNPs) on the micropropagation and promotion of desirable morpho-anatomical features in cloned plantlets of Santalum album (Santalaceae) have been conducted. In vitro generated shoots were cultivated on Murashige and Skoog’s (MS) medium containing 0.5 to 3.0 mg L− 1 of SiNPs with pre-opt...

The poor rooting of in vitro-raised shootlets is a major bottleneck and severely affects the survival rate of plantlets in field conditions. Hedyotis biflora is used as a model plant to study the impact of Silicon nanoparticles (SiNPs) on in vitro adventitious root formation and their structural developments in this study. The in vitro proliferated...

Thunbergia erecta (Benth.) T. Anderson (Acanthaceae) is an ornamental shrub. Viable seeds are not produced due to genetic incompatibility constraining propagation. An in vitro culture method is developed for the production of plants for the species. Surface-sterilized explants exhibited bud breaking on Murashige and Skoog (MS) medium + 2.0 mg L⁻¹ 6...

Authors report the application of silver nanoparticles (AgNPs) in the plant tissue culture system for effective and reproducible in vitro propagation of Gaillardia pulchella cv. ‘Torch Yellow’ for the year-round production of quality planting materials for the first time. Murashige and Skoog (MS) medium augmented with 1.0 mg L⁻¹ of 6-benzylaminopur...

A novel and effective direct shoot regeneration technique from stomatal complexes was developed for a highly sought after medicinal plant Coleus forskohlii. Meta-Topolin was more efficient than 6-benzylaminopurine (BAP) in promoting direct organogenesis from leaf surface. Highest direct organogenesis was achieved from in vitro leaf explants on Mura...

Vitex negundo L. (Lamiaceae) is an aromatic species used to cure several illnesses worldwide. The plant is a panacea of herbal formulations with several of its metabolites exhibiting significant pharmacological properties. The present study is an attempt to find the impact of silicon nanoparticles (SiNPs) on inducing in vitro flowering in V. negund...

Genus Crinum L. is a member of the Amaryllidaceae family having beautiful, huge, ornamental plants with umbels of lily-like blooms that are found in tropical and subtropical climates all over the world. For thousands of years, Crinum has been used as a traditional medicine to treat illnesses and disorders. Numerous distinct alkaloids of the Amaryll...

Gardenia jasminoides J. Ellis (Cape jasmine) is a horticultural plant. It is difficult to cultivate species due to pathogen sensitivity; hence, the demand for this species is gradually increasing. Thus, micropropagation would be a convenient tool to produce quality planting material, but the higher rate of mortality constitutes a constraint to larg...

The present study aimed to determine the foliar micro-morpho-anatomical features of in vitro cultured Gardenia jasminoides J. Ellis (Rubiaceae) to compare the effect of exogenous supplementation of growth regulators (cytokinins; 6-benzylaminopurine and Kinetin), in order to attenuate heterotrophic nutrition (in vitro) induced structural disorders i...

Caralluma umbellata Haw. is a rare and endemic xerophyte with astonishing therapeutic properties. It is used as an appetite suppressant and in weight loss management program. The natural population of this plant is decreasing due to overharvesting from the forests as an ethnomedicine. Therefore, an effective in vitro propagation system was develope...

Substantial progress has been made in the micropropagation of orchid species using biotechnological interventions, but the commercial viability of the technique has been hindered by increased mortality of plantlets out of culture vessels. This is attributed to the development of in vitro-induced structural and physiological abnormalities in the pla...

Vanilla planifolia – a natural source of vanillin is the world's highest-valued spice crop with endangered status and the only hand-pollinated crop globally. The present study evaluates the effect of exogenous silver nitrate (AgNO3) on effective direct and indirect somatic embryogenesis in V. planifolia for the first time. The direct and indirect s...

The present study was intended to develop an effective micropropagation system of the economically important ‘Grand Nain’ variety of Musa acuminata Colla (AAA) having high structural fidelity and significant potential to multiply shoots under in vitro conditions using the non-conventional cytokinin 6-benzoyladenine (BOA, C12H9N5O). The young sword...

Crinum malabaricum Lekhak & Yadav is a recently discovered and critically endangered aquatic bulbous plant of the family Amaryllidaceae. It gained attention as a wild source of the acetylcholinesterase inhibiting alkaloid ‘galanthamine’ used to treat Alzheimer and Parkinson diseases. The bulbs of this plant contain the highest amount of galanthamin...

Velamentous roots of orchids are desiccation resistant and help in anchorage and absorption of water and nutrients. The specialized multi-layered, spongy epidermis ‘velamen radicum’ is meant for epiphytic adaptation in economically and ecologically significant ‘grey orchid’ Vanda tessellata. The development of velamen tissue during various stages o...

An in vitro regeneration method with functional morpho-structural and physiological traits has been optimized for Aloe vera L. in the present study. Among the various types and treatments of cytokinins [6-benzylaminopurine, BAP; 6-(3-hydroxybenzylamino) purine, mT (meta-topolin), and 1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea, TDZ] tested for shoot in...

Lippia javanica is a multipurpose medicinal plant of the Verbenaceae family, which is commonly known for its volatile and essential oil compounds with numerous pharmaceutical applications. Due to medicinal importance, the plant was overexploited, which has been resulted in the rare availability of plant material. Through this study, an efficient an...

In vitro propagation is extremely important for the rapid multiplication of a wide range of orchids. This study aimed to investigate the effects of polyethylene glycol (PEG) on morpho-anatomical improvements and ex vitro development of Spathoglottis plicata, a terrestrial orchid. Various concentrations of PEG (100-500 mg L⁻¹) were incorporated with...

Key message The anatomical and histochemical localization at subsequent stages of somatic embryogenesis in Santalum album revealed the direct development of somatic embryos from internode explants. AbstractSantalum album L. (Santalaceae) is a vulnerable tree species due to overexploitation for commercial, pharmaceutical and cosmetic industries, whi...

The current investigation revealed a highly proficient and reproducible method for comprehensive in vitro regeneration and structural differentiation of bulblets in Crinum malabaricum Lekhak & Yadav using meta-topolin [mT; 6-(3-hydroxybenzylamino) purine] [6-benzylaminopurine] for the first time. Cultures were established from twin scales explants...

Best Oral Presentation Award ICEACBS 2022

The present investigation describes the efficacy of aromatic and synthetic cytokinins, which influence the direct organogenesis, biochemical responses, and ex vitro and in vivo performance of the in vitro propagated plantlets of Dioscorea pentaphylla L., an underrated famine tuber crop. The axillary shoot bud induction was achieved on the Murashige...

Santalum album L. (Indian sandalwood) is an economically important but vulnerable tropical tree species. Cultures were established via direct shoot regeneration from axillary buds on Murashige and Skoog (MS) medium supplemented with 2.5 mg L⁻¹ 6-benzylaminopurine (BAP). The shoots were multiplied using MS medium containing 1.0 mg L⁻¹ BAP and 0.5 mg...

The present report elucidates the foliar micro-morphological adaptation mechanism of micropropagated plantlets of Aerva lanata. The in vitro induced anomalies in the leaves were repaired through gradual hardening and acclimatization processes in a greenhouse. The epidermal tissues of in vitro grown leaves significantly differed from the field trans...

Oxystelma esculentum (L. f.) Sm. (Apocynaceae) is a perennial medicinal climber, enormously explored in traditional and modern systems of medicines. Due to over-harvesting from the wild, this species is categorized as ‘rare’. Hence, this study was aimed to develop an in vitro regeneration system for O. esculentum as a conservation measure. Nodal ex...

An improved in vitro propagation system has been developed for Santalum album L. using meta-Topolin for the first time. Direct organogenesis (100 %) was achieved by induction of axillary buds and a maximum of 8.2 shoots with 4.0 cm length was developed from nodal explants on Murashige and Skoog’s (MS) medium containing 1.0 mg L⁻¹ meta-Topolin (mT)....

Plantlets of Hemidesmus indicus (L.) R. Br. (Indian sarasaparilla) were developed in vitro in two different types of culture vessels and closure types-(1) culture bottles with polypropylene screw-caps and (2) conical flasks with cotton plugs both containing Murashige and Skoog medium with 1.0 mg L −1 6-benzylaminopurine, 0.5 mg L −1 kinetin, and 0....

An effective plant regeneration protocol via a liquid culture system has been ascertained in Spathoglottis plicata Blume (Orchidaceae), an important orchid of the horticulture industry. The seeds were germinated asymbiotically on the agar-gelled Murashige and Skoog’s (MS) medium supplemented with 1.0 mg L− 1 6-benzylaminopurine (BAP) and about 93.0...

Dendrocalamus strictus (Roxb.) Nees (family: Poaceae) is an economically important bamboo species explored worldwide for its wider adaptability. In vitro propagation is an alternate platform and has astonishing benefits in large-scale production of disease-free clones of the important plant species. Despite several efforts implicated to improve the...

An advanced micropropagation protocol has been developed for the global spice crop Vanilla planifolia using meta-topolin [mT, 6-(3-hydroxybenzylamino) purine] for the first time. Among the two types of cytokinins [mT and BAP (6-benzylaminopurine)] experimented, healthy shoot development and growth in terms of shoot numbers (5.0 ± 0.20/explant) with...

Spathoglottis plicata Blume is a vulnerable orchid species in various parts of the world, and the conventional propagation provides limited success to its cultivation and conservation. Therefore, present study deals with the direct induction of somatic embryos (SEs) from the leaf explants of S. plicata. Murashige and Skoog’s (MS) medium fortified w...

Origanum majorana (L.) (family Lamiaceae) is a multipurpose medicinal plant with high demand across the pharmaceutical, cosmetic, food, beverage, and perfume industries that has raised concern for its mass propagation through in vitro culture techniques. The present report describes an effective in vitro propagation system for an aromatic medicinal...

Micropropagation techniques allow producing large numbers of clones of genetically identical plants. However, there is evidence of disorders in internal structures due to sophisticated in vitro conditions. Such variations are responsible for the mortality of plantlets in the field and cause huge loss to the tissue culture industry. Anatomical evalu...

Vanda tessellata (Roxb.) Hook. ex G. Don. (Orchidaceae) is an epiphytic orchid species, commonly known as grey orchid, and explored enormously for its significant horticultural, medicinal, phytochemical and pharmacological properties. The present study describes an in vitro culture approach via asymbiotic seed germination assisted by phytohormones...

Ferocactus peninsulae (A.A.Weber) Britton & Rose (Barrel cactus) belongs to the family Cactaceae, has a great potential for commercial exploitation by the horticulture industry and landscape projects. It is difficult to propagate vegetatively because of slow and columnar growth; hence, its natural population is decreasing. Therefore, it is indeed d...

Ferocactus peninsulae (A.A.Weber) Britton & Rose (Cactaceae) has great potential for commercial exploitation by the horticulture industry. It is difficult to propagate vegetatively, and we investigated novel propagation methods. The objectives were to optimize the organic supplements in nutrient medium for seed germination, synthetic seed (synseeds...

Scaevola taccada (Gaertn.) Roxb. is a hemi-sclerophyllous littoral shrub of the family Goodeniaceae. It is a salt-tolerant plant and used in soil reclamation and coastal landscaping programs to control beach erosion. The present article reports a reproducible in vitro regeneration system using meta-topolin (mT) through liquid medium employing nodal...

The present communication reports, induction of adventitious roots (AR) from the in vitro raised leaves of Morinda coreia as affected by auxins and their varying concentrations. The roots of this plant possess anthraquinone, a preferable natural dye, explored well in pharmaceuticals. The AR from the leaves were induced under light and dark conditio...

Ormocarpum sennoides (bone-knit) is a vulnerable medicinal plant species belongs to the family Leguminosae. The aim of the present study was to evaluate the influence of plant growth regulators (PGRs), additives (ascorbic acid, citric acid, adenine sulfate, and L-arginine), gelling agents (agar and phytagel™) and type of culture vessels on the quan...

Vanda tessellata (Roxb.) Hook. ex G.Don (grey orchid) is a horticultural and medicinally potent, and conservation prioritized orchid species. The present study investigates asymbiotic seed germination, callus induction, somatic embryogenesis, encapsulation of somatic embryos (SEs), and retrieval of plantlets of V. tessellata from the artificial see...

Aloe vera (L.) Burm.f (Barbados aloe) is an economically important succulent herb, well accredited for its medicinal properties for centuries. In vitro propagation encouraged the production of identical individuals within a short period. However, there are evidences of in vitro induced disorders in the morphology, anatomy, phyto-chemistry and physi...

Present study explores the potential of seismo-mechanical stress in alleviating the macro- and micro-morphological aberrations observed in the in vitro regenerated plantlets of Vitex negundo L. (Chaste tree). Untreated in vitro plantlets showed non-functional stomata, trichomes, and photosynthetic pigments. This study demonstrates that these struct...

The micropropagation of critically endangered plant Crinum malabaricum Lekhak and Yadav (Malabar river lily) resulted in the development of in vitro-induced structural and physiological aberrations in the plantlets, which hampered the successful acclimatization and field establishment. Therefore, this study aimed to identify the effects of seismic...

Vanda tessellata (Roxb.) Hook. ex G. Don. (grey orchid, family Orchidaceae) is an epiphytic orchid of horticultural importance and currently under threat due to overharvesting and habitat destruction. Micropropagation protocols were developed for the production of grey orchid but the survival success of in vitro regenerated plantlets is uncertain d...

Application of seismo-mechanical stress in attenuation of in vitro induced abnormalities in Scaevola taccada (Gaertn.) Roxb. A B S T R A C T Seismic stress interplayed in the alleviation of in vitro induced morphometric and biochemical disorders and effective acclimatization of plantlets of Scaevola taccada. The in vitro regenerated plantlets (thro...

Hemidesmus indicus (L.) R. Br. ex Schult. (Indian Sarsaparilla of the family Apocynaceae) is a perennial climber used in traditional and modern medicinal systems since ages due to presence of a phytoconstituent MBAlD (2-hydroxy 4-methoxy benzaldehyde) in its roots. The morphological and light microscopic examination of the foliar appendages of the...

Development of nontoxic and clean techniques for the synthesis of silver nanoparticles (Ag NPs) has attracted increasing attention in recent years. The biological synthesis of silver nanoparticles using Turnera ulmifolia plant leaf extract was studied in present investigation. The synthesized nanoparticles were confirmed by color changes from pale...

The biogenesis of nanoparticles recently gained more attention. Here, we report the biogenesis of zinc oxide (ZnO) nanoparticles using aqueous extracts of the leaves, stem, root, flowers and fruits of Croton bonplandianum Baill. at room temperature. Croton bonplandianum is an important medicinal plant used to cure many pathological conditions in th...

The present communication reports the biosynthesis of zinc oxide nanoparticles using aqueous extracts of leaves, stem, root, flowers and fruits of Duranta erecta L. This plant gains horticultural and medicinal importance due to its various biological activities. Zinc Nitrate Hexahydrate [Zn (NO 3) 2 ·6H 2 O] solution was used as a precursor to synt...

The present study focuses on the green synthesis of zinc oxide nanoparticles using leaf, stem, root and flower extracts of Thunbergia erecta. Zinc nitrate hexahydrate (Zn (NO3)2.6H2O) solution was used as precursor to synthesize the nanoparticles. Five grams of plant materials (leaf, stem, root and flowers) were weighed and cut in to small pieces a...

Crinum malabaricum Lekhak & Yadav (Malabar river lily of the family Amaryllidaceae) is a critically endangered plant species and a natural source of galanthamine. Synthetic seeds production via somatic embryo-genesis was adopted as in vitro conservation strategy and proliferation of Malabar river lily in this study. Murashige and Skoog's (MS) basal...

Bambusa balcooa Roxb. is one of the economically most important bamboo species cultivated in the tropical countries. The present investigation is conceded to establish an effective method for clonal propagation of B. balcooa. The morphological response from nodal cuttings was tested on Murashige and Skoog's (MS) basal medium mixed with a range of c...

Oldenlandia herbacea (L.) Roxb. is an important medicinal plant of Indian, Malayan, and Chinese traditional systems of medicines. In vitro flowering system serves as a model system to study flowering physiology, and role of combination of photoperiodic conditions and ethylene inhibitors. Ethylene is responsible for delayed flowering response in pla...

A sustainable direct somatic embryogenesis protocol and consequent plant regeneration has been developed for the first time in Euphorbia hypericifolia L. a potential medicinal plant species. In vitro raised shoots from nodal shoot explants were subjected to attain somatic embryogenic potential in the present study. Murashige and Skoog's (MS) medium...

An effective in vitro regeneration protocol is essential to improve the natural population of conservationprioritized plants species. The micropropagation techniques are considered cost-effective if the survival chance of tissue-cultured plants is excellent in field conditions. Comparative foliar micromorphometric characteristics were analysed in...

An effective in vitro regeneration protocol developed for Oldenlandia herbacea. Comparative foliar micromorphometric characteristics were analysed to determine the sequential developmental adaptations of foliages of Oldenlandia herbacea plantlets under in vitro and field conditions. The leaf constants showed considerable variations in stomata and r...

Plant growth regulators (PGRs) may alter the morphogenic behavior of plant cells under in vitro conditions. Cytokinins and auxins were used to examine the response of mature nodal meristems of Oldenlandia herbacea (L.) Roxb. in this report. The 6-benzylaminopurine (BAP) alone at 1.5 mg L− 1 in Murashige and Skoog’s medium (MS) formed 5.0 ± 0.25 axi...

The in vitro induced anomalies during micropropagation affect acclimatization process of the regenerants, which influence the survival success of tissue culture raised plantlets. The comparative foliar micro-morphological analysis of in vitro developed; ex vitro adapted and field transferred plantlets could help in understanding the acclimatization...

Scaevola taccada (Gaertn.) Roxb. (family Goodeniaceae) is a large littoral shrub of great economic and ecological importance. Plantlets were regenerated from the in vitro raised leaf explants via direct somatic embryogenesis in this study. Nodes were cultured on Murashige and Skoog's (MS) medium augmented with 1.5 mg L ⁻¹ 6-benzylaminopurine (BAP)...

This report establishes the production and characterization of anthraquinones and phenolic compounds from the adventitious root cultures of Morinda coreia, induced from the leaves on half strength Murashige and Skoog’s (MS) medium fortified with 1.0 mg l⁻¹ of Indole-3 butyric acid (IBA). The in vitro regenerated adventitious roots (AR) were treated...

This study intended to develop a significant in vitro regeneration protocol for sustainable propagation, conservation and re-establishment of critically endangered aquatic plant species Crinum malabaricum Lekhak & Yadav (Malabar river lily). This plant is the natural source of galanthamine, the drug to treat Alzheimer’s disease. We present a scient...

A method for micropropagation of Coccoloba uvifera (L.) L. has been developed through bud proliferation from surface-sterilized nodal explants collected from a 9 yr old mature tree. Ninety six percent of the explants exhibited bud development on agar-gelled Murashige and Skoog's (MS) medium supplemented with 3.0 mg L⁻¹ 6-benzylaminopurine (BAP) if...

The current study provides an enhanced and reproducible micropropagation system in Artemisia vulgaris L. using nodal explants of mature plants. The explants were cultured on basal Murashige and Skoog's (MS) medium augmented with 6-benzylaminopurine (BAP) and Kinetin (Kin) concentrations individually. Direct regeneration of shoots (7.7 ± 0.36 shoots...

The present study describes successful in vitro regeneration systems in Chromolaena odorata (L.) King & H. Rob. by forced axillary bud break from nodal explants. Full strength Murashige and Skoog’s (MS) medium augmented with BAP at 8.88 μM resulted in maximum induction of shoots (4.6±0.25 shoots per explant). The shoots were promoted to proliferate...

Hedyotis biflora (Linn.) Lam is an important herb, used in the Indian and Chinese traditional systems of medicines. Indis- criminate harvesting of this plant resulted in decline of its population from the natural habitats. The present study reports effective micropropagation and in vitro flowering in H. biflora. Healthy plants were maintained in th...

A frugal method (use of Foldscope) was used to study the foliar micromorphological characteristics of Oxalis corniculata L. using foldscope in the present research. The results revealed the presence of hypostomatic, paracytic, anisocytic and anomocytic types of stomata. Stomatal density and index were observed as 71.8 and 31.5 respectively. The ven...

Justicia betonica L., family Acanthaceae is a widely used traditional folk medicinal herb. It is a unique source of jusbetonin and the leaves yield bluish purple dye. Traditionally, the plant is used to cure constipation, diarrhea, malaria, orchitis, pain, snake bite, stomach ache, vomiting etc. The whole plant possess significant biologically acti...

The present study was aimed to study and document the indigenous herbal knowledge of an ethnic community residing at Salem district (India) that was applied for their health complaints. The ethnobotanical exploration and documentation was conducted at Kurumbapatti, Palamalai, Kathrimalai, Sundaikkadu, Periyathanda, Kolathur, Komburankadu and Veerak...

Introduction:This paper reports on aquatic/semi-aquatic plants from the Siddha Medicinal Plants Garden (SMPG),Mettur Dam, Salem district of Tamil Nadu, which are used to cure various diseases in Siddha system of medicine. Materials and Methods: In the present review, information on Siddha formulations of the plants and medicinal properties along wi...

The ethnic people have their own culture, customs, religion and medical lores gained from their ancestors. The Kani tribal community settled in the forests of Agasthiyamalai of Western Ghats in Kanyakumari and Tirunelveli districts of Tamil Nadu and some places of Kerala also have their own culture and medical lores. Residing inside the forest area...