M. Manokari

M. Manokari
Central Council for Research in Siddha (M/o AYUSH) India

Doctor of Philosophy
In vitro regeneration-RET, AYUSH Medicinal &Aromatic plants. Plant structural & physiological behaviors from lab to land

About

136
Publications
36,328
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Citations
Introduction
Dr. M. Manokari, Asst. Research Officer, Central Council for Research in Siddha (M/o AYUSH), Plant biotechnologist (Biotech Research Unit, KMGIPSR, Pondicherry University), Scientific specialization: Conservation and micropropagation of RET, AYUSH & Horticultural species using biotechnological interventions, secondary metabolites, adv. root culture, in vitro structural and functional biology of plants, SE, Microscopy, Histochemical analysis & Biogenesis of nanoparticles.
Additional affiliations
June 2017 - present
Central Council for Research in Siddha (M/o AYUSH) Govt. of India
Position
  • Research Officer
Education
October 2012 - February 2016
Pondicherry University
Field of study
  • Plant Tissue Culture
August 2011 - October 2012
Kanchi Mamunivar Centre for Post Graduate Studies
Field of study
  • Plant Biology and Biotechnology
August 2009 - August 2011
Kanchi Mamunivar Centre for Post Graduate Studies
Field of study
  • Plant Biology and Biotechnology

Publications

Publications (136)
Article
Vanilla planifolia – a natural source of vanillin is the world's highest-valued spice crop with endangered status and the only hand-pollinated crop globally. The present study evaluates the effect of exogenous silver nitrate (AgNO3) on effective direct and indirect somatic embryogenesis in V. planifolia for the first time. The direct and indirect s...
Article
The present study was intended to develop an effective micropropagation system of the economically important ‘Grand Nain’ variety of Musa acuminata Colla (AAA) having high structural fidelity and significant potential to multiply shoots under in vitro conditions using the non-conventional cytokinin 6-benzoyladenine (BOA, C12H9N5O). The young sword...
Article
Full-text available
Lippia javanica is a multipurpose medicinal plant of the Verbenaceae family, which is commonly known for its volatile and essential oil compounds with numerous pharmaceutical applications. Due to medicinal importance, the plant was overexploited, which has been resulted in the rare availability of plant material. Through this study, an efficient an...
Article
Full-text available
Velamentous roots of orchids are desiccation resistant and help in anchorage and absorption of water and nutrients. The specialized multi-layered, spongy epidermis ‘velamen radicum’ is meant for epiphytic adaptation in economically and ecologically significant ‘grey orchid’ Vanda tessellata. The development of velamen tissue during various stages o...
Article
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An in vitro regeneration method with functional morpho-structural and physiological traits has been optimized for Aloe vera L. in the present study. Among the various types and treatments of cytokinins [6-benzylaminopurine, BAP; 6-(3-hydroxybenzylamino) purine, mT (meta-topolin), and 1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea, TDZ] tested for shoot in...
Article
In vitro propagation is extremely important for the rapid multiplication of a wide range of orchids. This study aimed to investigate the effects of polyethylene glycol (PEG) on morpho-anatomical improvements and ex vitro development of Spathoglottis plicata, a terrestrial orchid. Various concentrations of PEG (100-500 mg L⁻¹) were incorporated with...
Article
Full-text available
Key message The anatomical and histochemical localization at subsequent stages of somatic embryogenesis in Santalum album revealed the direct development of somatic embryos from internode explants. AbstractSantalum album L. (Santalaceae) is a vulnerable tree species due to overexploitation for commercial, pharmaceutical and cosmetic industries, whi...
Article
Full-text available
The current investigation revealed a highly proficient and reproducible method for comprehensive in vitro regeneration and structural differentiation of bulblets in Crinum malabaricum Lekhak & Yadav using meta-topolin [mT; 6-(3-hydroxybenzylamino) purine] [6-benzylaminopurine] for the first time. Cultures were established from twin scales explants...
Conference Paper
Full-text available
Best Oral Presentation Award ICEACBS 2022
Article
The present investigation describes the efficacy of aromatic and synthetic cytokinins, which influence the direct organogenesis, biochemical responses, and ex vitro and in vivo performance of the in vitro propagated plantlets of Dioscorea pentaphylla L., an underrated famine tuber crop. The axillary shoot bud induction was achieved on the Murashige...
Article
Full-text available
Santalum album L. (Indian sandalwood) is an economically important but vulnerable tropical tree species. Cultures were established via direct shoot regeneration from axillary buds on Murashige and Skoog (MS) medium supplemented with 2.5 mg L⁻¹ 6-benzylaminopurine (BAP). The shoots were multiplied using MS medium containing 1.0 mg L⁻¹ BAP and 0.5 mg...
Article
The present report elucidates the foliar micro-morphological adaptation mechanism of micropropagated plantlets of Aerva lanata. The in vitro induced anomalies in the leaves were repaired through gradual hardening and acclimatization processes in a greenhouse. The epidermal tissues of in vitro grown leaves significantly differed from the field trans...
Article
Full-text available
Oxystelma esculentum (L. f.) Sm. (Apocynaceae) is a perennial medicinal climber, enormously explored in traditional and modern systems of medicines. Due to over-harvesting from the wild, this species is categorized as ‘rare’. Hence, this study was aimed to develop an in vitro regeneration system for O. esculentum as a conservation measure. Nodal ex...
Article
An improved in vitro propagation system has been developed for Santalum album L. using meta-Topolin for the first time. Direct organogenesis (100 %) was achieved by induction of axillary buds and a maximum of 8.2 shoots with 4.0 cm length was developed from nodal explants on Murashige and Skoog’s (MS) medium containing 1.0 mg L⁻¹ meta-Topolin (mT)....
Article
Plantlets of Hemidesmus indicus (L.) R. Br. (Indian sarasaparilla) were developed in vitro in two different types of culture vessels and closure types-(1) culture bottles with polypropylene screw-caps and (2) conical flasks with cotton plugs both containing Murashige and Skoog medium with 1.0 mg L −1 6-benzylaminopurine, 0.5 mg L −1 kinetin, and 0....
Article
An effective plant regeneration protocol via a liquid culture system has been ascertained in Spathoglottis plicata Blume (Orchidaceae), an important orchid of the horticulture industry. The seeds were germinated asymbiotically on the agar-gelled Murashige and Skoog’s (MS) medium supplemented with 1.0 mg L− 1 6-benzylaminopurine (BAP) and about 93.0...
Chapter
Dendrocalamus strictus (Roxb.) Nees (family: Poaceae) is an economically important bamboo species explored worldwide for its wider adaptability. In vitro propagation is an alternate platform and has astonishing benefits in large-scale production of disease-free clones of the important plant species. Despite several efforts implicated to improve the...
Article
Full-text available
An advanced micropropagation protocol has been developed for the global spice crop Vanilla planifolia using meta-topolin [mT, 6-(3-hydroxybenzylamino) purine] for the first time. Among the two types of cytokinins [mT and BAP (6-benzylaminopurine)] experimented, healthy shoot development and growth in terms of shoot numbers (5.0 ± 0.20/explant) with...
Article
Full-text available
Spathoglottis plicata Blume is a vulnerable orchid species in various parts of the world, and the conventional propagation provides limited success to its cultivation and conservation. Therefore, present study deals with the direct induction of somatic embryos (SEs) from the leaf explants of S. plicata. Murashige and Skoog’s (MS) medium fortified w...
Article
Origanum majorana (L.) (family Lamiaceae) is a multipurpose medicinal plant with high demand across the pharmaceutical, cosmetic, food, beverage, and perfume industries that has raised concern for its mass propagation through in vitro culture techniques. The present report describes an effective in vitro propagation system for an aromatic medicinal...
Article
Micropropagation techniques allow producing large numbers of clones of genetically identical plants. However, there is evidence of disorders in internal structures due to sophisticated in vitro conditions. Such variations are responsible for the mortality of plantlets in the field and cause huge loss to the tissue culture industry. Anatomical evalu...
Article
Vanda tessellata (Roxb.) Hook. ex G. Don. (Orchidaceae) is an epiphytic orchid species, commonly known as grey orchid, and explored enormously for its significant horticultural, medicinal, phytochemical and pharmacological properties. The present study describes an in vitro culture approach via asymbiotic seed germination assisted by phytohormones...
Article
Full-text available
Ferocactus peninsulae (A.A.Weber) Britton & Rose (Barrel cactus) belongs to the family Cactaceae, has a great potential for commercial exploitation by the horticulture industry and landscape projects. It is difficult to propagate vegetatively because of slow and columnar growth; hence, its natural population is decreasing. Therefore, it is indeed d...
Article
Ferocactus peninsulae (A.A.Weber) Britton & Rose (Cactaceae) has great potential for commercial exploitation by the horticulture industry. It is difficult to propagate vegetatively, and we investigated novel propagation methods. The objectives were to optimize the organic supplements in nutrient medium for seed germination, synthetic seed (synseeds...
Article
Full-text available
Scaevola taccada (Gaertn.) Roxb. is a hemi-sclerophyllous littoral shrub of the family Goodeniaceae. It is a salt-tolerant plant and used in soil reclamation and coastal landscaping programs to control beach erosion. The present article reports a reproducible in vitro regeneration system using meta-topolin (mT) through liquid medium employing nodal...
Article
The present communication reports, induction of adventitious roots (AR) from the in vitro raised leaves of Morinda coreia as affected by auxins and their varying concentrations. The roots of this plant possess anthraquinone, a preferable natural dye, explored well in pharmaceuticals. The AR from the leaves were induced under light and dark conditio...
Article
Full-text available
Vanda tessellata (Roxb.) Hook. ex G.Don (grey orchid) is a horticultural and medicinally potent, and conservation prioritized orchid species. The present study investigates asymbiotic seed germination, callus induction, somatic embryogenesis, encapsulation of somatic embryos (SEs), and retrieval of plantlets of V. tessellata from the artificial see...
Article
Ormocarpum sennoides (bone-knit) is a vulnerable medicinal plant species belongs to the family Leguminosae. The aim of the present study was to evaluate the influence of plant growth regulators (PGRs), additives (ascorbic acid, citric acid, adenine sulfate, and L-arginine), gelling agents (agar and phytagel™) and type of culture vessels on the quan...
Article
Aloe vera (L.) Burm.f (Barbados aloe) is an economically important succulent herb, well accredited for its medicinal properties for centuries. In vitro propagation encouraged the production of identical individuals within a short period. However, there are evidences of in vitro induced disorders in the morphology, anatomy, phyto-chemistry and physi...
Article
Full-text available
Present study explores the potential of seismo-mechanical stress in alleviating the macro- and micro-morphological aberrations observed in the in vitro regenerated plantlets of Vitex negundo L. (Chaste tree). Untreated in vitro plantlets showed non-functional stomata, trichomes, and photosynthetic pigments. This study demonstrates that these struct...
Article
The micropropagation of critically endangered plant Crinum malabaricum Lekhak and Yadav (Malabar river lily) resulted in the development of in vitro-induced structural and physiological aberrations in the plantlets, which hampered the successful acclimatization and field establishment. Therefore, this study aimed to identify the effects of seismic...
Article
Vanda tessellata (Roxb.) Hook. ex G. Don. (grey orchid, family Orchidaceae) is an epiphytic orchid of horticultural importance and currently under threat due to overharvesting and habitat destruction. Micropropagation protocols were developed for the production of grey orchid but the survival success of in vitro regenerated plantlets is uncertain d...
Article
Application of seismo-mechanical stress in attenuation of in vitro induced abnormalities in Scaevola taccada (Gaertn.) Roxb. A B S T R A C T Seismic stress interplayed in the alleviation of in vitro induced morphometric and biochemical disorders and effective acclimatization of plantlets of Scaevola taccada. The in vitro regenerated plantlets (thro...
Article
Hemidesmus indicus (L.) R. Br. ex Schult. (Indian Sarsaparilla of the family Apocynaceae) is a perennial climber used in traditional and modern medicinal systems since ages due to presence of a phytoconstituent MBAlD (2-hydroxy 4-methoxy benzaldehyde) in its roots. The morphological and light microscopic examination of the foliar appendages of the...
Research
Full-text available
Development of nontoxic and clean techniques for the synthesis of silver nanoparticles (Ag NPs) has attracted increasing attention in recent years. The biological synthesis of silver nanoparticles using Turnera ulmifolia plant leaf extract was studied in present investigation. The synthesized nanoparticles were confirmed by color changes from pale...
Research
Full-text available
The biogenesis of nanoparticles recently gained more attention. Here, we report the biogenesis of zinc oxide (ZnO) nanoparticles using aqueous extracts of the leaves, stem, root, flowers and fruits of Croton bonplandianum Baill. at room temperature. Croton bonplandianum is an important medicinal plant used to cure many pathological conditions in th...
Research
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The present communication reports the biosynthesis of zinc oxide nanoparticles using aqueous extracts of leaves, stem, root, flowers and fruits of Duranta erecta L. This plant gains horticultural and medicinal importance due to its various biological activities. Zinc Nitrate Hexahydrate [Zn (NO 3) 2 ·6H 2 O] solution was used as a precursor to synt...
Research
Full-text available
The present study focuses on the green synthesis of zinc oxide nanoparticles using leaf, stem, root and flower extracts of Thunbergia erecta. Zinc nitrate hexahydrate (Zn (NO3)2.6H2O) solution was used as precursor to synthesize the nanoparticles. Five grams of plant materials (leaf, stem, root and flowers) were weighed and cut in to small pieces a...
Article
Crinum malabaricum Lekhak & Yadav (Malabar river lily of the family Amaryllidaceae) is a critically endangered plant species and a natural source of galanthamine. Synthetic seeds production via somatic embryo-genesis was adopted as in vitro conservation strategy and proliferation of Malabar river lily in this study. Murashige and Skoog's (MS) basal...
Article
Bambusa balcooa Roxb. is one of the economically most important bamboo species cultivated in the tropical countries. The present investigation is conceded to establish an effective method for clonal propagation of B. balcooa. The morphological response from nodal cuttings was tested on Murashige and Skoog's (MS) basal medium mixed with a range of c...
Article
Oldenlandia herbacea (L.) Roxb. is an important medicinal plant of Indian, Malayan, and Chinese traditional systems of medicines. In vitro flowering system serves as a model system to study flowering physiology, and role of combination of photoperiodic conditions and ethylene inhibitors. Ethylene is responsible for delayed flowering response in pla...
Conference Paper
Full-text available
A sustainable direct somatic embryogenesis protocol and consequent plant regeneration has been developed for the first time in Euphorbia hypericifolia L. a potential medicinal plant species. In vitro raised shoots from nodal shoot explants were subjected to attain somatic embryogenic potential in the present study. Murashige and Skoog's (MS) medium...
Article
Full-text available
An effective in vitro regeneration protocol is essential to improve the natural population of conservationprioritized plants species. The micropropagation techniques are considered cost-effective if the survival chance of tissue-cultured plants is excellent in field conditions. Comparative foliar micromorphometric characteristics were analysed in...
Article
An effective in vitro regeneration protocol developed for Oldenlandia herbacea. Comparative foliar micromorphometric characteristics were analysed to determine the sequential developmental adaptations of foliages of Oldenlandia herbacea plantlets under in vitro and field conditions. The leaf constants showed considerable variations in stomata and r...
Article
Plant growth regulators (PGRs) may alter the morphogenic behavior of plant cells under in vitro conditions. Cytokinins and auxins were used to examine the response of mature nodal meristems of Oldenlandia herbacea (L.) Roxb. in this report. The 6-benzylaminopurine (BAP) alone at 1.5 mg L− 1 in Murashige and Skoog’s medium (MS) formed 5.0 ± 0.25 axi...
Article
The in vitro induced anomalies during micropropagation affect acclimatization process of the regenerants, which influence the survival success of tissue culture raised plantlets. The comparative foliar micro-morphological analysis of in vitro developed; ex vitro adapted and field transferred plantlets could help in understanding the acclimatization...
Article
Scaevola taccada (Gaertn.) Roxb. (family Goodeniaceae) is a large littoral shrub of great economic and ecological importance. Plantlets were regenerated from the in vitro raised leaf explants via direct somatic embryogenesis in this study. Nodes were cultured on Murashige and Skoog's (MS) medium augmented with 1.5 mg L ⁻¹ 6-benzylaminopurine (BAP)...
Article
This report establishes the production and characterization of anthraquinones and phenolic compounds from the adventitious root cultures of Morinda coreia, induced from the leaves on half strength Murashige and Skoog’s (MS) medium fortified with 1.0 mg l⁻¹ of Indole-3 butyric acid (IBA). The in vitro regenerated adventitious roots (AR) were treated...
Article
Full-text available
This study intended to develop a significant in vitro regeneration protocol for sustainable propagation, conservation and re-establishment of critically endangered aquatic plant species Crinum malabaricum Lekhak & Yadav (Malabar river lily). This plant is the natural source of galanthamine, the drug to treat Alzheimer’s disease. We present a scient...
Article
A method for micropropagation of Coccoloba uvifera (L.) L. has been developed through bud proliferation from surface-sterilized nodal explants collected from a 9 yr old mature tree. Ninety six percent of the explants exhibited bud development on agar-gelled Murashige and Skoog's (MS) medium supplemented with 3.0 mg L⁻¹ 6-benzylaminopurine (BAP) if...
Article
The current study provides an enhanced and reproducible micropropagation system in Artemisia vulgaris L. using nodal explants of mature plants. The explants were cultured on basal Murashige and Skoog's (MS) medium augmented with 6-benzylaminopurine (BAP) and Kinetin (Kin) concentrations individually. Direct regeneration of shoots (7.7 ± 0.36 shoots...
Article
The present study describes successful in vitro regeneration systems in Chromolaena odorata (L.) King & H. Rob. by forced axillary bud break from nodal explants. Full strength Murashige and Skoog’s (MS) medium augmented with BAP at 8.88 μM resulted in maximum induction of shoots (4.6±0.25 shoots per explant). The shoots were promoted to proliferate...
Article
Hedyotis biflora (Linn.) Lam is an important herb, used in the Indian and Chinese traditional systems of medicines. Indis- criminate harvesting of this plant resulted in decline of its population from the natural habitats. The present study reports effective micropropagation and in vitro flowering in H. biflora. Healthy plants were maintained in th...
Chapter
Full-text available
A frugal method (use of Foldscope) was used to study the foliar micromorphological characteristics of Oxalis corniculata L. using foldscope in the present research. The results revealed the presence of hypostomatic, paracytic, anisocytic and anomocytic types of stomata. Stomatal density and index were observed as 71.8 and 31.5 respectively. The ven...
Article
Full-text available
Justicia betonica L., family Acanthaceae is a widely used traditional folk medicinal herb. It is a unique source of jusbetonin and the leaves yield bluish purple dye. Traditionally, the plant is used to cure constipation, diarrhea, malaria, orchitis, pain, snake bite, stomach ache, vomiting etc. The whole plant possess significant biologically acti...
Article
The present study was aimed to study and document the indigenous herbal knowledge of an ethnic community residing at Salem district (India) that was applied for their health complaints. The ethnobotanical exploration and documentation was conducted at Kurumbapatti, Palamalai, Kathrimalai, Sundaikkadu, Periyathanda, Kolathur, Komburankadu and Veerak...
Article
Introduction:This paper reports on aquatic/semi-aquatic plants from the Siddha Medicinal Plants Garden (SMPG),Mettur Dam, Salem district of Tamil Nadu, which are used to cure various diseases in Siddha system of medicine. Materials and Methods: In the present review, information on Siddha formulations of the plants and medicinal properties along wi...
Article
The ethnic people have their own culture, customs, religion and medical lores gained from their ancestors. The Kani tribal community settled in the forests of Agasthiyamalai of Western Ghats in Kanyakumari and Tirunelveli districts of Tamil Nadu and some places of Kerala also have their own culture and medical lores. Residing inside the forest area...
Article
Full-text available
Green synthesis of Zinc oxide nanoparticles (ZnO-NPs) is a novel and non-toxic method as compared to the hazardous conventional physical and chemical methods. Herein, we report production of ZnO-NPs for the first time using whole vegetative parts of Sesamum indicum L. The aqueous extracts of various parts of S. indicum were used to synthesize nanop...
Article
Full-text available
Plant tissue culture techniques offer quick methods of regeneration of plants of medicinal importance but the survival chances of such plants are always questionable when shifted to the in vivo conditions. The present study enumerates the micromorphological developments in the leaves of in vitro regenerated and field transferred plantlets of Oldenl...
Article
Full-text available
The present report illustrates an improved and reproducible micropropagation system for economically valuable bamboo species Pseudoxytenanthera stocksii using nodal segments. Direct organogenesis from nodal segments was accomplished by culturing on Murashige and Skoog (MS) medium supplemented with 6.0 mg/L 6-benzylaminopurine (BAP). On this medium...
Article
Full-text available
Biological synthesis of metal nanoparticles gained worldwide interest due to their rapid, non-toxic, economical, single-step technique and eco-friendly alternative. Green-chemical approach of production of Zinc Oxide (ZnO) nanoparticles (NPs) has been exploited in the field of medicine, food packaging, nano-optical and electrical devices. Muntingia...
Article
Oldenlandia umbellata L. gains importance due to its medicinal properties and the presence of anthraquinones based natural dyes in the roots. Present study describes the effect of Murashige and Skoog’s (MS) liquid medium (full strength) on in vitro regeneration, flower bud induction and ex vitro rooting in O. umbellata. Shoot segments with 2-3 node...