M. Camino Ordás

M. Camino Ordás
Instituto de Investigaciones Agropecuarias | INIA · Animal Health Research Centre

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25
Publications
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Publications

Publications (25)
Article
β-glucans are widely used in fish diets as immunomodulators to enhance the defensive capacities of farmed animals and improve their resistance to pathogens. Despite their extensive use, the precise effects that β-glucans provoke on immune and intestinal cells in teleost fish have not yet been fully elucidated. In the current work, we have used the...
Article
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a multifunctional neuropeptide belonging to the glucagon/secretin superfamily. In teleost fish, PACAP has been demonstrated to have an immunomodulatory role. Although previous studies have shown that viral/bacterial infections can influence the transcription of PACAP splicing variants an...
Article
Although viruses represent a major threat for cultured fish worldwide, the commercialization of vaccines capable of providing effective and long-lasting protection is still lacking for most of these viral diseases. In this situation, the use of supplemented diets could be a suitable strategy to increase the immune status of the fish and reduce the...
Article
A survey of immune-relevant genes that might be up-regulated in response to viral hemorrhagic septicaemia virus (VHSV) in the rainbow trout monocyte-macrophage cell line, RTS11, unexpectedly revealed an increased expression of perforin (PRF) and granzyme (GRZ) genes, which represent components of the major cytotoxic pathway. The natural killer-enha...
Article
Although many chemokine genes have been identified in rainbow trout (Oncorhynchus mykiss) as in other teleost species, almost no studies focused on their biological role have been conducted, despite the fact that no clear inferences as to their functions can be made based on their low similarity to mammalian counterparts. In the current work, we ha...
Article
Full-text available
The fin bases constitute the main portal of rhabdovirus entry into rainbow trout (Oncorhynchus mykiss), and replication in this first site strongly conditions the outcome of the infection. In this context, we studied the chemokine response elicited in this area in response to viral hemorrhagic septicemia virus (VHSV), a rhabdovirus. Among all the r...
Article
A laboratory experiment was carried out to study immune function alteration of the mussel Mytilus galloprovincialis when exposed to the Prestige oil spilled in November 2002 on the northwestern Spanish coast. Mussels were maintained for 4 months in tanks with flowing seawater and with 1, 2, and 0 kg (controls) Prestige fuel oil. Polycyclic aromatic...
Article
The tumor necrosis factor (TNF) superfamily is composed by several proteins with similar structure and functions. One of the main representatives of this family is TNF-alpha (TNFalpha), a proinflammatory cytokine which is produced by different immune cells and presents a wide variety of activities. Using the RACE technique, we have cloned and seque...
Article
The interferon regulatory factor (IRF) family comprises transcription factors that regulate the expression of interferon and interferon-related cytokines. Using the RACE technique, we have determined the complete cDNA sequence of turbot (Scophthalmus maximus) and sea bream (Sparus aurata) IRFs. These sequences shared characteristics with other IRFs...
Article
In 1999, abnormal mortalities of cockle (Cerastoderma edule) were reported in Galicia (NW Spain). We found several potentially pathogenic organisms and cellular disorders, including neoplasic cells with a strong lysosomic activity, which could be associated with the reported cockle mortalities.
Article
Full-text available
The genus Perkinsus includes protistan parasites infecting marine molluscs throughout the world, some of which are associated with mass mortalities. Life cycle involves vegetative proliferation within the host, by which a cell named trophozoite undergoes successive bipartitioning. Other stages have been observed in vitro or in vivo, depending on th...
Article
Nineteen strains of Gymnodinium catenatum were isolated from one bloom in Andalucía (S Spain) and from different blooms in Galicia (NW Spain). The PSP toxin profiles of 16 of the strains were analyzed, and although the saxitoxin was exclusive to the Galician strains, the corresponding dendogram showed no clustering of the isolates from this locatio...
Article
The development of hypnospores (large cells that stain dark blue with Lugol's iodine) after incubation of bivalves tissues in Ray's fluid thioglycollate medium (RFTM) has been used for Perkinsus diagnosis for the last 40 years. Polymerase chain reaction (PCR) was performed using conserved 18S rRNA gene primers on DNA from an enriched preparation of...
Article
Several pathogenic protozoan release proteases are necessary for host invasion and initiation of infection. We have identified proteolytic activities in extracellular proteins secreted by the clam parasite Pseudoperkinsus tapetis (Mesomycetozoa) in vitro. The protein concentration of the P. tapetis extracellular products (ECP) increased only during...
Article
In this study, we have determined the histopathology of the infection by the protozoan parasite Perkinsus atlanticus in three exploitable clam species (carpet-shell clam Ruditapes decussatus, Manila clam R. philippinarum, and the species R. pullastra) from Galicia (NW of Spain). In histological preparations from infected animals, typical mature tro...
Article
Defence parameters of non-infected clams (Ruditapes decussatus) and clams heavily infected with Perkinsus atlanticus were assessed. Cellular (haemocyte density and phagocytic activity) and humoral (lysozyme and anti-bacterial activities, protein concentration and agglutination titre) parameters were measured in clams collected in an area enzootic f...
Article
The influence of several factors on the chemiluminescence (CL) activity of haemocytes from the Mediterranean mussel (Mytilus galloprovincialis) was studied. Haemocytes were stimulated in vitro with different concentrations of zymosan, phorbol 12-myristate 13-acetate (PMA) and lipopolysaccharide (LPS) (adding superoxide dismutase, SOD, to the zymosa...
Article
Parasites identified as Perkinsus atlanticus have been reported infecting carpet shell clams in Galicia (northwest Spain). We have sequenced the 18S ribosomal RNA gene of in vitro cultured Perkinsus atlanticus-like or hypnospores from diseased clams, and compared it with the same genomic region from P. marinus and Perkinsus sp. We have also compare...
Article
The in vitro effects of the secretion products (SP) released by cultured Perkinsus atlanticus on the phagocytic activity of two shellfish species, Ruditapes decussatus and Mytilus galloprovincialis were determined. Haemocytes from clam and mussel were incubated with Perkinsus SP, and the number of phagocytosed particles were counted. The particles...
Article
Full-text available
The ability to mass-culture parasites in vitro facilitates biological, metabolic and morpho logical research that would otherwise be difficult to accomplish. In vitro continuous culture of the protistan Perkinsus atlanticus from carpet-shell clam was established and variables including culture medium, inoculum size, temperature and salinity, which...

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