M. Angela A. Meireles

University of Campinas | UNICAMP · Departamento de Engenharia de Alimentos (DEA)

This study investigated the technological properties of barley hydrocolloids to produce a kinetically stable plant-based non-dairy alternative milk. The impact of mechanical extraction assisted by electrolysis treatments (0, 5, and 15 V) on the barley protein content was examined. The protein-rich barley aqueous extract was used to emulsify canola...

The development of active packaging for food storage containers is possible through impregnation of natural extracts by supercritical CO2-assisted impregnation processes. The challenge of scCO2-impregnation of natural extracts is to control the total loading and to ensure that the composition of the loaded extract may preserve the properties of the...

Emerging technologies have been investigated as smart process options for manufacturing plant-based non-dairy alternative milk. However, few studies have evaluated the effects of these non-conventional technologies on the preservation of the bioactive and nutritional compounds. In this regard, this study aimed to examine the impact of pulsed high-p...

In this paper, genipin, an important natural crosslinker and anti-neurodegenerative compound, was extracted from unripe Genipa americana L., combining high-intensity ultrasound (HIUS) and natural deep eutectic solvents (NADESs). The extraction process conditions were evaluated step-by-step to reach the best genipin recovery. The obtained ready-to-u...

Tannins are a class of molecules belonging to the group of phenolic compounds widely found in plant matter and have attracted great attention due to their applications related to several biological activities. The interest in recovering and concentrating these compounds has increased in recent years, especially due to their potential application in...

The thermosonication processing has been evaluated as a smart alternative to overcoming the heat treatment limitations associated with longer holding times at high temperatures. In this regard, this innovative technique combines acoustic energy with moderate heat treatments to inactivate pathogenic and spoilage microorganisms and endogenous enzymes...

Whey beverages that were enriched with fructooligosaccharides (FOS) and xylooligosaccharides (XOS) were used for carrying Foeniculum vulgare extract that was obtained by the supercritical CO2 extraction technique to produce novel functional products. Fennel-based whey beverages were subjected to thermosonication processing (100, 200, and 300 W at 6...

This study aimed to valorize pomegranate by-products (peel and carpelar membranes—PPCM) through their high biological potential for phenolic compounds recovery. The influence of lower temperatures (40 and 60 °C) and pressures (20, 40, 60, 80, and 100 bar) than those generally used in pressurized liquid extraction (PLE) was evaluated through global...

Currently, one of the biggest challenges of the colorant industry is to obtain natural blue colorants. Among the different options, a blue pigment can be formed by a crosslinking reaction between genipin and primary amine groups. However, at the industrial level, obtaining an ingredient from pure compounds, such as amino acids, is economically unfe...

Supercritical fluid extraction (SFE) assisted by cold pressing (SFEAP) was previously reported in the literature for oleaginous raw materials to improve the SFE process, but it suffers from operational disadvantages due to its sequential, instead of simultaneous, extraction operations. Therefore, this study aimed to address this issue by simultaneo...

This study aimed to evaluate the impact of the ultrasound intensity (0, 5, 10, and 20 W/cm²) on the esterification of annatto (Bixa orellana L.) seed starch with octenyl succinic anhydride (OSA) employing a short processing time (5 min) to produce a novel emulsifier. OSA-esterified annatto seed starches were examined according to their degree of su...

This chapter provides a scenario from the past 3 years on the extraction of polyphenols using sub- and supercritical fluid-based technologies, including supercritical fluid extraction (SFE) and pressurized liquid extraction (PLE). The nonpolar behavior of CO2 does not allow the extraction of most polyphenols. Therefore using cosolvents is necessary...

This study aimed to examine the impact of the combination of acoustic energy at the nominal powers of 100, 200, 300, and 400 W with moderate heat processing at 40, 50, and 60 °C on the extraction of phytochemical compounds from Foeniculum vulgare. Thermosonication processing, based on high-intensity ultrasound combined with an external heat source,...

Supercritical CO2 extraction of sucupira branca (SB) seeds was studied under different conditions of pressure (20-40 MPa) and temperature (40 and 60°C). Operational conditions were assessed on laboratory and pilot scales, and their influences on manufacturing cost (COM) and productivity were discussed. At 40°C and 30 MPa for 65 min, 40 g oil/100 g...

Consumers are demanding food products with high added value concerning their sensory properties and safety. In this scenario, updates in industrial food processing have been claimed by the consumer market due to the undesirable sensory properties associated with severe heat treatments. Therefore, the development of novel food processes based on inn...

The growing interest of industrialized nations expressed through academics and industry in reducing the negative impact on the environment has motivated the search for new processes simulation tools, studied from raw materials to the production of a finished product. Currently, these tools make its possible to measure the positive impact when the s...

Consumers are demanding not animal-derived food products produced by clean technologies. An almond-based functional beverage enriched with coconut oil and dietary fibers and colored with natural blue colorant was formulated and processed by low-frequency ultrasound combined with mild heat treatment. This study aimed to evaluate the effects of therm...

Xylooligosaccharides (XOS) are emerging prebiotic ingredients used for enriching food products due to their health-promoting properties. This study investigated XOS chemical stability after high-pressure processing (HPP) combined with heat treatment. First, orange juice was enriched with XOS (xylobiose, xylotriose, xylotetraose, xylopentaose, and x...

Ultrasound-assisted extraction (UAE) of semi-defatted unripe genipap (SDG) using supercritical CO2 was performed to enhance the recovery of natural colorant iridoids genipin and geniposide. There are currently few natural sources of iridoids, and their application as colorants is scarce. The UAE resulted in extracts with blue and green colors using...

Xylooligosaccharides (XOS) are emerging prebiotic carbohydrates with a high potential to promote health and wellness due to their ability to modulate gut microbiota. Their use as a carrier of milk proteins-crosslinked genipin, an innovative natural blue colorant, was examined in this study. Fructooligosaccharides (FOS), a standard prebiotic carbohy...

The production of an innovative food ingredient obtained from unripe Genipa Americana L. pulp and milk was studied in this paper. Natural blue compounds were produced from the crosslinking between genipin and milk proteins. They are a promising ingredient for the natural food colorant sector. In this way, this paper aimed to evaluate the impact of...

Inulin-type fructans with different degrees of polymerization (DPs) were used as wall materials for the blue colorant produced from the crosslinking between genipin and milk proteins. The impact of using fructooligosaccharides (FOS) with DP = 5 and inulins with DP ≥ 10 (GR-In) and DP ≥ 23 (HP-In) on the physical (microstructure, size, water activit...

This study aimed to evaluate the effects of the high-intensity ultrasound (HIUS) on the recovery of phenolic compounds from the leaves, fruit pulp, and seed of Eugenia calycina Cambess. The impact of using different modes of application of the same ultrasound-specific energy (kJ/g) was examined. Ultrasound energy performance was assessed employing...

In the last decades, different non-thermal and thermal technologies have been developed for food processing. However, in many cases, it is not clear which experimental parameters must be reported to guarantee the experiments’ reproducibility and provide the food industry a straightforward way to scale-up these technologies. Since reproducibility is...

Background The food industry seeks to expand the color palette with natural colorants. Nevertheless, in nature, the blue color is rare compared to other colors. Despite synthetic colorants' effects on human health, synthetic blue colorants are largely used by the food industry. In this way, consumers usually associate the blue color with artificial...

Aims This work aims to understand the effects of processing variables on supercritical fluid extraction from Mauritia flexuosa (Aguaje). This is not a cultivar because the plants used are indigenous. Background The production of Mauritia flexuosa (Aguaje) is an economically significant activity in Madre de Dios, Peru, which has rarely been studied...

Phytochemicals extracted from natural sources are widely studied for many applications, including the prevention of diseases and functional foods. In this context, passion fruit by-products (PFBP) can play an important role in obtaining these compounds. This work aimed to concentrate PFBP extracts from super-critical fluid extraction (SFE) (fixed a...

This study investigated the impact of adding β-carotene on the structure of fresh O/W emulsions with different oil phase (sunflower oil-LCT or NEOBEE®1053-MCT) and emulsifiers (WPI, Tween 80 – T80 or WPI/T80 mixture). In this sense, the behavior of emulsions through the gastrointestinal tract, the stability and bioaccessibility of β-carotene were a...

This work presents an economic evaluation of supercritical fluid extraction (SFE) process from uvaia leaves. Extractions were performed at a previously optimized condition of pressure (200 bar) and temperature (60 °C) and were evaluated with the addition of 5% of two different co-solvents (ethyl acetate and ethanol). A scale-up study was performed...

Red peppers (Capsicum annuum L.) that are eliminated as waste due to its low quality for commercialization were used as raw material to recover bioactive compounds in a biorefinery approach. The effect of temperature (40, 60 °C) and pressure (15, 25, 35 MPa) was evaluated on the oleoresin extraction yield and carotenoids composition obtained by sup...

Monguba seeds show high content of extractable oil, approximately 54% (w/w). Literature data have reported the recovery of monguba seed oil using conventional methods. However, the use of emerging and environment-friendly methods should be applied, especially the supercritical fluid process. Therefore, we evaluated herein the effect of supercritica...

Buritirana (Mauritiella armata Mart.) is a fruit species native to the Amazon and Cerrado region, belonging to the Arecaceae family. It has high nutritional and functional potential, yet little explored. In this study, we evaluated for the first time the overall yield, behavior of total carotenoids in the extraction kinetics, fatty acid profile, bi...

The increase in the consumption of natural and non-dairy products has boosted the worldwide colorant food industry. Thus, the use of non-animal protein sources to produce natural blue compounds from the cross-linking between primary amines and genipin extracted from unripe Genipa americana L. were evaluated in this study. Plant extracts obtained fr...

The present study evaluated the effect of supercritical fluid extraction (SFE) assisted by cold pressing (SFEAP) on the overall yield, extraction kinetics, composition of baru seed oil and manufacturing cost (COM). The best extraction conditions were determined in extraction assays combining different pressures (150–350 bar) and temperatures (35 an...

The goal of green analytical chemistry is to implement efficient methods with reduced use of toxic solvents and minimum generation of wastes streams. The use of emerging extraction technologies (ultrasound- and microwave-assisted, pressurized liquid and supercritical fluid extraction) to prepare samples for analytical detection increased because of...

This chapter covers an updated review of the health and technological aspects of lycopene. The consumption of processed foods was correlated to the highest absorption of lycopene in the human body because of the isomerization of trans-lycopene to cis-lycopene induced by processing parameters such as temperature and pressure. Most of the published w...

Haematococcus pluvialis is the largest producer of natural astaxanthin in the world. Astaxanthin is a bioactive compound used in food, feed, nutraceutics, and cosmetics. In this study, astaxanthin extraction from H. pluvialis by supercritical fluid extraction was evaluated. The effects of temperature (40 and 50 °C), pressure (40 and 50 MPa), and CO...

We report a mathematical model of the supercritical fluid foaming (SCF) from the poly(ε-caprolactone) (PCL) + CO2 system based on the Laws of Conservation of Momentum and Conservation of Mass. The main model assumptions are constant temperature, negligible inertial effects, Newtonian and incompressible fluid, equal-sized bubble, constant bubbles de...

This paper presents techno-economic results for the high-intensity ultrasound (HIUS)-assisted extraction of a valuable phytochemical. Genipin is a precussor of natural blue compounds besides presenting anticancer, anti-oxidative, and anti-inflammatory properties. The effects of the non-thermal and thermal HIUS treatments on obtaining of genipin-ric...

The effects of supercritical CO2 processing on the chemical stability of fructooligosaccharides (FOS) and other functional and nutritional compounds were evaluated employing non-thermal and thermal approaches. Apple juice was enriched with Pfaffia glomerata roots aqueous extract due to its high content of short-chain FOS and then subjected to diffe...

The aim of this study was to evaluate the extraction of α-bisabolol from different parts of candeia wood (bark, core and core + bark) using supercritical CO2. The extractions were performed evaluating two levels of temperature (313 and 333 K) and pressure (10 and 20 MPa). The extraction yield ranged from 0.76 to 2.6 g/100 RM (raw material), while t...

The use of supercritical CO2 (SC–CO2) as a solvent for the extraction of vanillin from organosolv media was proposed and evaluated. The use of sugarcane bagasse as a biomass source to product diversification has been gaining much attention recently and it is a very promising research topic. From sugarcane bagasse, it is already produced fuels as 2G...

This work investigates a novel approach for turmeric rhizomes valorization for the obtaining of microparticles composed of curcuminoids ethanolic extract and fractionated volatile oils, using compressed carbon dioxide as an antisolvent and the recovery of phenolic compounds and carbohydrates with pressurized hot water. In addition, a cheap and vers...

This chapter discusses the recent (2013–2018) applications on the use of supercritical CO2 (SC–CO2) antisolvent for micronization, coprecipitation, and fractionation of high-value products for the food, cosmetic, and pharmaceutical industries, most focusing on the applications of integrated techniques on the biorefining of plant matrices into marke...

The use of supercritical CO2 (SC–CO2) antisolvent for micronization, coprecipitation, and fractionation of high-value products for biorefining of plant matrices into marketable products has been a promising and increasing research topic. These SC–CO2 antisolvent processes are able to microencapsulate many materials that are difficult to treat with...

This chapter proposes an innovative biorefinery conceptual process for the production of carotenoids from microalgae using lignocellulose-based biorefinery products and/or by-products and pressurized fluids. The extraction process, which can be done also with microalgal biomass with high content of moisture avoiding high-cost downstream processes,...

This study was organized into three experimental steps. In the first step, SFE was used for the extraction of the fatty acid-rich extract from unripe genipap. The extract with the highest yield (4.6 ± 0.1%) and total fatty acid content (16.6 mg fatty acids / g) was obtained at 30 MPa and 333 K. In the second step, the kinetic behavior of the overal...

The aim of this study was to evaluate the effects of non-thermal and thermal high-intensity ultrasound (HIUS) treatment on the microbial and enzymatic inactivation, physicochemical properties, and kinetic stability of the raw milk by applying different energy densities (1, 3, 5, and 7 kJ/mL). Two HIUS treatments were evaluated based on different no...

Tecnologias de extração, micronização e estabilização de compostos bioativos por encapsulação em matrizes poliméricas utilizando fluidos pressurizados podem representar uma alternativa ambientalmente correta, uma vez que estão incluídas no conceito de "química verde" e do desenvolvimento sustentável, e economicamente viável em relação aos respectiv...

Inulin is a prebiotic carbohydrate widely used in the food industry due to its health-related claims and its ability to modify/improve the technological properties of food products. However, industrial processes such as thermal treatments, can cause structure changes in prebiotic carbohydrates affecting their health benefits and technological prope...

A promising natural blue colorant was obtained from the cross-linking between genipin and milk proteins. Milk was simultaneously used to extract genipin from the unripe genipap (Genipa americana L.) and evaluated as a reaction medium and carrier for the novel blue colorant. The effects of the milk composition (skimmed, semi-skimmed, and whole) on t...

Background The new global trends for consuming natural products rich in bioactive compounds and health-promoter phytochemicals have increased the modern consumer's interest in fruit and vegetable juices. But, the current technologies based on thermal treatments reduce the nutritional value and degrade sensory attributes of these products in relatio...

Chañar is a typical fruit of arid and semiarid regions of South America, commonly used to produce sweet honey-like syrup called “arrope” from its pulp. During the arrope production, the chañar almonds that contain elevated amount of oil are disposed as a residue. Thus, the aim of this study was to extract the chañar almond oil by supercritical flui...

This study presents the production of a novel natural blue colorant obtained from the cross-linking between milk proteins and genipin assisted by low-frequency and high-power ultrasound technology. Genipin was extracted from unripe Genipa americana L. using milk as a solvent. Also, milk colloidal system was used as a reaction medium and carrier for...

Passion fruit bagasse is a rich source of phenolic compounds, including piceatannol, a stilbene to which several biological activities are conferred. This work reports the application of pressurized liquid extraction (PLE) assisted by ultrasound (US) to intensify the extraction of phenolic compounds from defatted passion fruit bagasse (DPFB). PLE a...

The effect of grinding time (GT) from 15 s to 20 min on the dynamic yield of supercritical fluid extraction (SFE( from fennel was investigated. The extractor was subjected to a pressure of 200 bar, a temperature of 313 K, and a supercritical CO2 flow rate of 2.0 × 10⁻⁴ kg/s over 80 min. It was found that the overall SFE yield was increased from 6.4...

This study evaluated the influence of the high-intensity ultrasound (HIUS) technology on the quality parameters of a model dairy beverage (chocolate whey beverage), operating under the same energy density (5000 J/mL), but applied at different ways. Two processes were performed varying nominal power and processing time: HIUS–A (160 W and 937 s), and...

In this study, a biorefinery for the processing of turmeric (Curcuma longa L.) based on clean and emerging technologies has been proposed. High-intensity ultrasound (HIUS) technology was evaluated as a promising technique for curcumin recovery aiming to improve its extraction yield and technological properties as a colorant. In addition, we evaluat...

This book provides deep insights about the fundamentals, applications and perspectives of the use of supercritical CO2 as solvent and antisolvent for biorefinering.

The effects of the high-intensity ultrasound (HIUS) technology at the nominal powers of 300, 600, 900, and 1200 W were evaluated on the chemical stability of xylooligosaccharides (XOS) used to enrich orange juice. The ultrasound energy performance for each nominal power applied to the XOS-enriched orange juice was determined by calculating acoustic...

Passion fruit bagasse extract (PFBE) is a rich source of polyphenols, including piceatannol. This work produced alginate (1, 2, 3 wt%) aerogel and investigated the impregnation of gallic acid (GA) and PFBE in alginate aerogel microparticles. The microparticles of ca. 100 μm in diameter were obtained by emulsion-gelation method, submitted to solvent...

The inulin is known as a prebiotic carbohydrate with beneficial health properties in addition to its technological properties as a texture modifier and stabilizer of food products. Despite that, a little is known about the influence of the degree of inulin polymerization during gastrointestinal digestion. Then, the objective of this study was to ev...

This work consists of the steps for the assembly of a Supercritical Antisolvent Precipitation laboratory equipment and evaluation of the parts acquisition costs. A flow diagram with all components was developed, a complete list of all necessary components was presented, and an estimate of the acquisition of these parts in Brazil was reported. The s...

Surfactants are widely used in the food industry to form and stabilize emulsion-based food and beverage products. The use of natural surfactant to replace the synthetic ones is a recent demand in industries. In this work, first it was evaluated the influence of hydrostatic pressure levels (up to 10 bars applying nitrogen), oily phase type and surfa...

Efficient encapsulation techniques and development of special delivery systems enhance the stability of target compounds, enabling their processing and application. Supercritical fluid extraction of emulsions (SFEE) is a promising alternative to process natural target compounds, due to its suitability to encapsulate poorly water-soluble compounds i...

High Turbulence Extraction Assisted by Ultrasound combined with Supercritical Antisolvent Fractionation (SAF) was done to intensify the recovery of bixin and phenolic compounds from semi-defatted annatto seeds. Annatto seeds are extensively used due to its colorant properties and bioactive potential in human health. Modifications were performed in...

The effects of several operational parameters (pressure, temperature, CO2 flow rate, solution flow rate, injector type, and concentration of solute in the ethanol solution) during Supercritical AntiSolvent (SAS) precipitation process on the energy consumption cost per unit of manufactured product were investigated using experimental design techniqu...