Lyudmyla V. Shkotova

National Academy of Sciences of Ukraine | ISP · Biomolecular Electronics

A bioselective membrane of the potentiometric sensor based on butyrylcholinesterase and pH-sensitive field-effect transistors (FETs) modified with gold nanoparticles (GNPs) have been developed. In the present investigation, we focused on the study and adjustment of the potentiometric biosensor just for the case of using gold nanoparticles because b...

Normal microflora of human skin and the development of its representatives depending on the skin pH are considered in this article. It is shown a possibility of using probiotic microorganisms of genera Bifidobacterium, Lactobacillus, Lactococcus, Bacillus and their metabolites for making the cosmetic medical remedies for different skin types. It wa...

The review presents the characteristics of bacteria of the Lactobacillus family and their ability to synthesize various bacteriocins. Also there is shown a classification of bacteriocins of lactobacilli, which includes three classes: class I – lantibiotics (peptides with a molecular weight of less than 5 kDa which contain lanthionine), class II – u...

The review presents the composition of goat colostrum, antimicrobial, immunomodulatory and antioxidant properties of biologically active proteins of goat colostrum, as well as the prospects of their use in medicine. Due to the presence of a complex of biologically active proteins such as lactoferrin, lysozyme, lactoperoxidase, immunoglobulins, etc....

Detection of the lactate content is of great importance in clinical diagnostics, fermentation industry, and control of the quality of food products. The work was aimed at the development of a sensitive element of the amperometric biosensor, based on enzyme lactate oxidase and carbon electrodes modified with platinum and palladium nanoparticles (Pt&...

Aim. To develop an amperometric biosensor based on glucose oxidase (1.1.3.4) from Aspergillus niger immobilized in the IrNPs/Ludox/GOx matrix for glucose detection. Methods. To achieve a highly selective and sensitive glucose detection, the enzymatic membrane was functionalized with Ir nanoparticles (IrNPs) and silica composite Ludox. The enzymatic...

To develop an amperometric biosensor based on immobilized uricase (1.7.3.3) from Arthrobacter Globiformis and a platinum disk electrode for the detection of uric acid in biological fluids. Methods. To obtain a highly selective detection of the uric acid concentration, an additive semi-permeable polymer film was formed on the surface of a platinum d...

The rapid development of nanotechnology has led to the expansion of its scope of application. Nanomaterials are used in various fields of science and technology: from aeronautics to micro- and nanoelectronics, from biotechnology up to genetic engineering. Application of nanomaterials and nanotechnology provides innovation boom in medicine. The arti...

The paper is a review of the researches of Biomolecular Electronics Laboratory concerning the development of biosensors based on electrochemical transducers (amperometric and conductometric electrodes, potentiometric pH-sensitive field effect transistors) and different biorecognition molecules (enzymes, cells, antibodies), biomimics (molecularly im...

The inspection method of the thin-film electrochemical transducers metrological characteristics based on surface condition assessment of the planar electrode system by value of parameters of equivalent circuits are described. The correlation of magnitude and stability of response of measuring channel with electrical equivalent circuit parameters is...

To improve analytical characteristics of amperometric biosensors for glucose determination, aminated and carboxylated multiwalled carbon nanotubes were applied. Attested samples of nanotubes were suspended and used for modification of amperometric transducers С220АТ of DropSens production and bioselective membranes. The glucose oxidase membranes we...

A comparative analysis of the efficiency of using glycerol oxidase preparations, which differ in their characteristics and methods of production, in the development of an amperometric biosensor for glycerol determination, was performed. The enzyme preparation which, being immobilized on the transducer surface, ensured the best working characteristi...

Abstract. The principles of construction of four-channel measuring-information system for work with multielectrode electrochemical cells, in particular, with amperometric multibiosensors have been considered. The basic hardware and software of the complex were described; results of its experimental measurements are given. Keywords: electrochemistry...

A lactate oxidase-based amperometric biosensor is designed for lactate determination. Two methods of lactate oxidase immobilization on the surface of commercial SensLab platinum printing electrodes are compared. The sensor with lactate oxidase immobilized by physical adsorption in Resydrol polymer is shown to have both narrower dynamic range (0.004...

The method of lactate determination in wine by amperometric biosensor based on platinum screen-printed electrode SensLab and lactate oxidase immobilized in polymer PEDT by electrochemical polymerization was optimized. Platinum screen-printed electrode SensLab without enzymatic membrane was demonstrated to show no response to wine, must and main win...

A new biosensor based on amperometric transducer and alcohol oxidase immobilised in Resydrol polymer for ethanol detection has been developed. The optimal composition and immobilisation conditions for creating active membrane based on alcohol oxidase have been determined. Electrochemical deposition of the polymer film has been achieved by applying...

The amperometric biosensor based on lactate oxidase for determination of lactate has been developed, and two methods of immobilization of lactate oxidase on the surface of industrial screen-printed platinum electrodes SensLab were compared. A sensor with immobilized in the Resydrol polymer lactate oxidase by the method of physical adsorption is cha...

The characteristics of different transducers for creation of amperometric biosensors such as various carbon and metallic electrodes were investigated. It was shown that the platinum is best electrode material for using as amperometric transducers. It is also possible to use interdigitated planar electrodes. It was shown that optimal material for su...

The amperometric biosensor for ethanol determination based on alcohol oxidase immobilised by the method of electrochemical polymerization has been developed. The industrial screen-printed platinum electrodes were used as transducers for creation of amperometric alcohol biosensor. Optimal conditions for electrochemical deposition of an active membra...

The amperometric enzyme biosensor was used for glucose analysis in grape wine. To decrease the influence of the samples under analysis on analytical characteristics of the biosensor the additional nafion membrane was deposited on the enzyme membrane. The sensor work was investigated in different conditions. It was shown that application of addition...

A glucose amperometric biosensor has been developed, its characteristics being studied. The stipulation of obtaining active membrane based on glucose oxidase has been optimized. The changes in both background electrolyte concentration and buffer capacity have been shown to affect the biosensor response insufficiently. The optimum pH is found to be...

A new improved technology for manufacturing microelectrodes based on carbon fibres has been developed. The microelectrodes produced are long living, stable to electrochemical and chemical treatment. The electrochemically activated microelectrodes demonstrated high sensitivity to H2O2 with the detection limit for hydrogen peroxide of 0.5 μM. The mic...

The microelectrodes based on carbon fibres have been developed. They are characterised in long service life and stability to electrochemical and chemical pre-treatment. Being electrochemically activated they demonstrate high sensitivity to H2O2 - the detection limit is 0.5 mM. The biosensors based on the carbon fibre microelectrodes have been devel...