Luning Liu

Luning Liu
University of Liverpool | UoL · Institute of Integrative Biology

PhD

About

117
Publications
17,275
Reads
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2,229
Citations
Introduction
I am a Professor and a Royal Society University Research Fellow at the University of Liverpool. Combining molecular genetics, biochemistry, synthetic biology and biophysics, I am interested in the molecular basis of protein distribution and assembly in biological machines, light-harvesting mechanism of the photosynthetic apparatus, and the physiological regulations of bioenergetic machinery.
Additional affiliations
October 2018 - present
University of Liverpool
Position
  • Professor
Description
  • Chair of Microbial Bioenergetics and Bioengineering
October 2013 - present
University of Liverpool
Position
  • Fellow
December 2012 - September 2018
University of Liverpool
Position
  • Principal Investigator

Publications

Publications (117)
Article
Full-text available
Bacterial metabolosomes are a family of protein organelles in bacteria. Elucidating how thousands of proteins self-assemble to form functional metabolosomes is essential for understanding their significance in cellular metabolism and pathogenesis. Here we investigate the de novo biogenesis of propanediol-utilization (Pdu) metabolosomes and characte...
Article
Full-text available
The reaction center (RC) and light-harvesting complex 1 (LH1) form a RC–LH1 core supercomplex that is vital for the primary reactions of photosynthesis in purple phototrophic bacteria. Some species possess the dimeric RC–LH1 complex with a transmembrane polypeptide PufX, representing the largest photosynthetic complex in anoxygenic phototrophs. How...
Article
Full-text available
A wide range of bacteria use special protein-based organelles, termed bacterial microcompartments, to encase enzymes and reactions to increase the efficiency of biological processes. As a model bacterial microcompartment, the carboxysome contains a protein shell filled with the primary carbon fixation enzyme Rubisco.
Preprint
Carboxysomes are a family of bacterial microcompartments in cyanobacteria and chemoautotrophs. It encapsulates carbonic anhydrase and Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) catalysing carbon fixation inside a proteinaceous shell. How Rubisco packs into the carboxysomes is unknown. Using cryo-electron tomography and subtomogram av...
Preprint
Carboxysomes are proteaceous bacterial microcompartments (BMCs) that sequester the key enzymes for carbon fixation in cyanobacteria and some proteobacteria. They consist of a virus-like icosahedral shell, encapsulating carbonic anhydrase and ribulose 1,5 bisphosphate carboxylase/oxygenase (RuBisCO), which catalyses the dehydration of bicarbonate in...
Preprint
Full-text available
The reaction center (RC) and light-harvesting complex 1 (LH1) form a RC-LH1 core supercomplex that is vital for the primary reactions of photosynthesis in purple photosynthetic bacteria. Some species possess the dimeric RC-LH1 complex with an additional polypeptide PufX, representing the largest photosynthetic complex in anoxygenic phototrophs. How...
Preprint
Full-text available
Bacterial metabolosomes are a family of protein organelles in bacteria. Elucidating how thousands of proteins self-assemble to form functional metabolosomes is essential for understanding their significance in cellular metabolism and pathogenesis. Here we investigate the de novo biogenesis of propanediol-utilization (Pdu) metabolosomes and characte...
Preprint
Full-text available
Carboxysomes are anabolic bacterial microcompartments that play an essential role in carbon fixation in cyanobacteria and some chemoautotrophs. This self-assembling organelle encapsulates the key CO 2 -fixing enzymes, Rubisco, and carbonic anhydrase using a polyhedral protein shell that is constructed by hundreds of shell protein paralogs. The α-ca...
Article
Full-text available
The carboxysome is a versatile paradigm of prokaryotic organelles and is a proteinaceous self-assembling microcompartment that plays essential roles in carbon fixation in all cyanobacteria and some chemoautotrophs. The carboxysome encapsulates the central CO2-fixing enzyme, ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), using a polyhedr...
Article
Bacterial microcompartments (BMCs) are self-assembling prokaryotic organelles consisting of a polyhedral proteinaceous shell and encapsulated enzymes that are involved in CO2 fixation or carbon catabolism. Addressing how the hundreds of building components self-assemble to form the metabolically functional organelles and how their structures and fu...
Article
Full-text available
Background QconCATs are quantitative concatamers for proteomic applications that yield stoichiometric quantities of sets of stable isotope-labelled internal standards. However, changing a QconCAT design, for example, to replace poorly performing peptide standards has been a protracted process. Results We report a new approach to the assembly and c...
Article
Full-text available
The light reactions of photosynthesis occur in thylakoid membranes that are densely packed with a series of photosynthetic complexes. The lateral organization and close association of photosynthetic complexes in native thylakoid membranes are vital for efficient light harvesting and energy transduction. Recently, analysis of the interconnections be...
Article
Full-text available
How thylakoid membranes are generated to form a metabolically active membrane network and how thylakoid membranes orchestrate the insertion and localization of protein complexes for efficient electron flux remain elusive. Here, we develop a method to modulate thylakoid biogenesis in the rod-shaped cyanobacterium Synechococcus elongatus PCC 7942 by...
Article
Full-text available
SbtA is a high-affinity, sodium-dependent bicarbonate transporter found in the cyanobacterial CO 2 -concentrating mechanism (CCM). SbtA forms a complex with SbtB, while SbtB allosterically regulates the transport activity of SbtA by binding with adenyl nucleotides. The underlying mechanism of transport and regulation of SbtA is largely unknown. In...
Article
Full-text available
The reaction center (RC)−light-harvesting complex 1 (LH1) supercomplex plays a pivotal role in bacterial photosynthesis. Many RC-LH1 complexes integrate an additional protein PufX that is key for bacterial growth and photosynthetic competence. Here, we present a cryo–electron microscopy structure of the RC-LH1-PufX supercomplex from Rhodobacter vel...
Preprint
How thylakoid membranes are generated to form the metabolically active membrane network and how thylakoid membranes orchestrate the insertion and localization of protein complexes for efficient electron flux remain elusive. Here, we develop a method to modulate thylakoid biogenesis in the rod-shaped cyanobacterium Synechococcus elongatus PCC 7942 b...
Preprint
Full-text available
We report a new approach to the assembly and construction of QconCATs, quantitative concatamers for proteomic applications that yield stoichiometric quantities of sets of stable isotope-labelled internal standards. The new approach is based on synthetic biology precepts of biobricks, making use of loop assembly to construct larger entities from ind...
Article
Full-text available
Bacterial metabolosomes have been discovered for over 25 years. They play essential roles in bacterial metabolism and pathogenesis. In this crystal ball paper, I will discuss the recent advances in the fundamental understanding and synthetic engineering of bacterial metabolosomes. Bacterial metabolosomes have been discovered for over 25 years. They...
Article
Full-text available
For the unicellular alga Chlamydomonas reinhardtii, the presence of N -glycosylated proteins on the surface of two flagella is crucial for both cell-cell interaction during mating and flagellar surface adhesion . However, it is not known whether only the presence or also the composition of N -glycans attached to respective proteins is important for...
Article
The N⁶-methyladenosine (m⁶A) modification is the most common internal post-transcriptional modification, with important regulatory effects on RNA export, splicing, stability, and translation. Studies on the m⁶A modifications in plants have focused on Arabidopsis thaliana growth and development. However, A. thaliana is a salt-sensitive and model pla...
Article
Full-text available
Compartmentalization is a ubiquitous building principle in cells, which permits segregation of biological elements and reactions. The carboxysome is a specialized bacterial organelle that encapsulates enzymes into a virus-like protein shell and plays essential roles in photo-synthetic carbon fixation. The naturally designed architecture, semi-perme...
Article
Full-text available
Abstract Bacterial microcompartments (BMCs) are nanoscale proteinaceous organelles that encapsulate enzymes from the cytoplasm using an icosahedral protein shell that resembles viral capsids. Of particular interest are the carboxysomes (CBs), which sequester the CO2-fixing enzymes ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) to enhance...
Article
Full-text available
Photosynthetic membranes are typically densely packed with proteins, and this is crucial for their function in efficient trapping of light energy. Despite being crowded with protein, the membranes are fluid systems in which proteins and smaller molecules can diffuse. Fluidity is also crucial for photosynthetic function, as it is essential for bioge...
Article
Full-text available
Carboxysomes are the specific CO2-fixing microcompartments in all cyanobacteria. Although it is known that the organization and subcellular localization of carboxysomes are dependent on external light conditions and are highly relevant to their functions, how carboxysome organization and function are actively orchestrated in natural diurnal cycles...
Article
Plant chloroplasts have complex membrane systems. Among these, thylakoids serve as the sites for photosynthesis and photosynthesis-related adaptation. In addition to the photosynthetic membrane complexes and associated molecules, lipids in the thylakoid membranes, are predominantly composed of MGDG (monogalactosyldiacylglycerol), DGDG (digalactosyl...
Article
Full-text available
Cell division of Staphylococcus adopts a “popping” mechanism that mediates extremely rapid separation of the septum. Elucidating the structure of the septum is crucial for understanding this exceptional bacterial cell division mechanism. Here, the septum structure of Staphylococcus warneri is extensively characterized using high-speed time-lapse co...
Preprint
Full-text available
Bacterial microcompartments (BMCs) are nanoscale proteinaceous organelles that encapsulate enzymes from the cytoplasm using an icosahedral protein shell that resembles viral capsids. Of particular interest are the carboxysomes (CBs), which sequester the CO2-fixing enzymes ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) to enhance carbon a...
Article
Full-text available
Cyanobacteria are autotrophs whose photosynthetic process is similar to that of higher plants, although the photosynthetic apparatus is slightly different. They have been widely used for decades as model systems for studying the principles of photosynthesis, especially the effects of environmental stress on photosynthetic activities. Salt stress, w...
Preprint
Full-text available
Cell division of Staphylococcus adopts a “popping” mechanism that mediates extremely rapid separation of the septum. Elucidating the structure of the septum is crucial for understanding this exceptional bacterial cell division mechanism. Here, the septum structure of Staphylococcus warneri is extensively characterized using high-speed time-lapse co...
Preprint
Full-text available
For the unicellular alga Chlamydomonas reinhardtii , the presence of N -glycosylated proteins on the surface of two flagella is crucial for both cell-cell interaction during mating and flagellar surface adhesion. It is unknown whether the composition of N -glycans attached to respective proteins is important for these processes. To this end, we exa...
Chapter
Carboxysomes are a group of bacterial microcompartments (BMCs) that encapsulate Rubisco and carbonic anhydrase to enhance CO2 fixation in cells. Through self-assembly of hundreds of proteins into a virus-like icosahedral organelle, carboxysomes provide all cyanobacteria and some chemoautotrophs with the ability to utilise limited environmental CO2...
Chapter
Purple photosynthetic bacteria are metabolically versatile, anoxygenic phototrophs that produce bacteriochlorophylls a or b and display a wide range of metabolic lifestyles, which are reflected in their diverse range of habitats. Under oxic conditions, energy is derived from aerobic respiration, and the synthesis of photosynthetic pigments, and pig...
Article
Full-text available
Long non-coding RNAs (lncRNAs) can enhance plant stress resistance by regulating the expression of functional genes. Sweet sorghum is a salt-tolerant energy crop. However, little is known about how lncRNAs in sweet sorghum respond to salt stress. In this study, we identified 126 and 133 differentially expressed lncRNAs in the salt-tolerant M-81E an...
Article
Full-text available
Precise folding of photosynthetic proteins and organization of multicomponent assemblies to form functional entities are fundamental to efficient photosynthetic electron transfer. The bacteriochlorophyll b-producing purple bacterium Blastochloris viridis possesses a simplified photosynthetic apparatus. The light-harvesting (LH) antenna complex surr...
Article
Full-text available
Sweet sorghum is a C4 crop with the characteristic of fast-growth and high-yields. It is a good source for food, feed, fiber, and fuel. On saline land, sweet sorghum can not only survive, but increase its sugar content. Therefore, it is regarded as a potential source for identifying salt-related genes. Here, we review the physiological and biochemi...
Article
Full-text available
Bicarbonate transporters play essential roles in pH homeostasis in mammals and photosynthesis in aquatic photoautotrophs. A number of bicarbonate transporters have been characterized, among which is BicA—a low-affinity, high-flux SLC26-family bicarbonate transporter involved in cyanobacterial CO2-concentrating mechanisms (CCMs) that accumulate CO2...
Article
Full-text available
The carboxysome is a complex, proteinaceous organelle that plays essential roles in carbon assimilation in cyanobacteria and chemoautotrophs. It comprises hundreds of protein homologs that self-assemble in space to form an icosahedral structure. Despite its significance in enhancing CO2 fixation and potentials in bioengineering applications, the fo...
Preprint
Full-text available
The carboxysome is a complex, proteinaceous organelle that plays essential roles in carbon assimilation in cyanobacteria and chemoautotrophs. It comprises hundreds of protein homologs that self-assemble in space to form an icosahedral structure. Despite its significance in enhancing CO 2 fixation and potentials in bioengineering applications, the f...
Article
Phycobiliproteins (PBPs) are the main component of light-harvesting complexes in cyanobacteria and red algae. In addition to their important role in photosynthesis, PBPs have many potential applications in foods, cosmetics, medical diagnosis and treatment of diseases. However, basic researches and technological innovations are urgently needed for e...
Article
Full-text available
Bacterial microcompartments (BMCs) are proteinaceous self-assembling organelles that are widespread among the prokaryotic kingdom. By segmenting key metabolic enzymes and pathways using a polyhedral shell, BMCs play essential roles in carbon assimilation, pathogenesis, and microbial ecology. The BMC shell is composed of multiple protein homologs th...
Article
Full-text available
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is the essential enzyme mediating the fixation of atmospheric CO2 during photosynthesis. In cyanobacteria, Rubisco enzymes are densely packed and encapsulated in a specialized organelle known as the carboxysome. Well-defined Rubisco assembly and carboxysome formation are pivotal for efficien...
Chapter
Full-text available
Bacterial microcompartments (BMCs) are polyhedral protein organelles in many prokaryotes, playing significant roles in metabolic enhancement. Due to their self-assembly and modularity nature, BMCs have gained increased interest in recent years, with the intent of constructing new nanobioreactors and scaffolding to promote cellular metabolisms and m...
Article
Full-text available
Bacterial microcompartments (BMCs) are proteinaceous organelles widespread among bacterial phyla and provide a means for compartmentalizing specific metabolic pathways. They sequester catalytic enzymes from the cytoplasm, using an icosahedral proteinaceous shell with selective permeability to metabolic molecules and substrates, to enhance metabolic...
Article
Full-text available
Incubation conditions are an important factor to consider when studying protein aggregation in vitro. Here, we employed biophysical methods and atomic force microscopy to show that agitation dramatically alters the morphology of medin, an amyloid protein deposited in the aorta. Agitation reduces the lag time for fibrillation by ~18-fold, suggesting...
Data
Document S1. Supplemental Methods, Supplemental Figures 1–10, Supplemental Table 1, and Supplemental Reference
Data
Data were recorded for 12 min and shown at 15 frames per second. Snapshots and kymograph are presented in Figure 8.
Data
Data were recorded for 12 min and shown at 15 frames per second. Snapshots and kymograph are presented in Figure 8.
Data
Data were recorded for 10 min and shown at 15 frames per second. Snapshots and kymograph are presented in Supplemental Figure 9.
Data
Data were recorded for 12 min and shown at 15 frames per second. Snapshots and kymograph are presented in Supplemental Figure 9.
Data
Data were recorded for 12 min and shown at 15 frames per second. Snapshots and kymograph are presented in Figure 8.
Data
Data were recorded for 10 min and shown at 15 frames per second. Snapshots and kymograph are presented in Supplemental Figure 9.
Data
Data were recorded for 12 min and shown at 15 frames per second. Snapshots and kymograph are presented in Supplemental Figure 9.