Luna Samanta

• Ph.D
• Pofessor at Ravenshaw University

Background The association between Vitamin D Receptor (VDR) polymorphisms and different cancers has attracted growing attention; nonetheless, the function of these genetic variants in tobacco-related oral cancer remains little comprehended. This review assesses and integrates research concerning the influence of VDR gene variants on the development...

Two triazine‐cored BODIPY trimers (Compounds 6 and 12) were synthesized and well characterized using ¹H NMR, ¹³C NMR, ¹¹B NMR, ¹⁹F NMR, FT‐IR, and high‐resolution mass spectral analysis. The photophysical, computational, and in vitro anticancer studies of the synthesized compounds were comprehensively evaluated along with previously reported phenyl...

In this study, we report the cardioprotective effect of the glycerol monooleate (GMO) based nanocurcumin in both in vitro and in vivo conditions under a hyperthyroid state. The heart is one of the primary target organs sensitive to the action of thyroid hormone, and slight variations in the thyroid hormone serum concentrations result in measurable...

Effect of priming on the maturation and spawning were tested in two fish species using gonadotropin-releasing hormone (GnRH), carp pituitary homogenate (CPH) and clomiphene citrate (clomid). The fish were injected with a dose of GnRH, CPH and clomid, calibrated to contain 0.3ml/kg, 7 mg/kg and 70mg/kg respectively in three split doses (two priming...

Despite significant progress in the recent past, cancer remains as an enigma for both clinicians and scientists alike and the underlying mechanism(s) of onset and progression of the disease remain unresolved. Nevertheless, in recent years several efforts have been crafted for use of high through put omics technologies including genomics (detection...

Despite significant progress in the recent past, cancer remains as an enigma for both clinicians and scientists alike and the underlying mechanism(s) of onset and progression of the disease remain unresolved.

A new series of 2H‐chromene‐based hydrazones (10 a–w, 12 a–d, and 14 a–d) were efficiently synthesized by the reaction of substituted 2H‐chromene aldehydes (8 a–w) with various hydrazines (9, 11, and 13) in absolute ethanol under microwave irradiation as well as by lemon juice mediated conventional heating. The structures of synthesized target mole...

The endangered Indian Narrow Headed Soft Shell Turtle Chitra indica, native to Indian subcontinent, is found in major rivers and their tributaries in Odisha. Over the past few decades, the population of Chitra indica has declined due to exploitation for the consumption of its meat and eggs, fishing activities, habitat destruction resulting from ill...

The end product of germ cell maturation is the spermatozoa that are produced through a definite pattern in nature that requires a special environment. During proliferation of diploid germ cells, the spermatogonia repopulate the testis lobules by mitotic cell division. The natural germ cell proliferation and maturation has been modulated and manipul...

Diabetes mellitus is one of the complex metabolic disorders associated with individuals leading sedentary lifestyles. It leads to several complications rendering the normal function of vital organs like heart, liver, kidney, eye and brain. Scientists and doctors across the globe are involved in research for understanding the complex genetics of thi...

Introduction: Polyaromatic hydrocarbons (PAHs) are considered as redox active environmental toxicants inducing oxidative stress (OS) mediated injury to cells. Oxidative predominance is reported in 30%–80% of idiopathic male infertility (IMI) patients. Hence, this work aims to unravel correlation, if any, between seminal PAH exposome and sperm funct...

Boron-rich glycodendrimers containing three to six peripheral hydrophilic glucose moieties and three ortho-carborane clusters have been synthesized. Adding more numbers of glucose moieties compensated the hydrophobicity of ortho-carboranes and made...

This work advocates that in situ crosslinked chitosan-dialdehyde guar gum (CsDAGG) hydrogels can be effectually used for dual drug release in colorectal cancer therapy. Herein, we designed various grades of CsDAGG hydrogels where DAGG was accomplished by periodate oxidation of guar gum. With increasing DAGG content, a diminution in swelling and a m...

An exponential growth in chronic non-healing wounds worldwide have propelled the dire need for the design of effectual interventions. Of late, dialdehyde polysaccharides have blossomed as novel antimicrobial agents. Yet, such dialdehydes, accomplished by periodate oxidation, are mostly synthesized by the conventional strategy. Microwave-assisted sy...

Despite having tremendous medicinal benefits, the practical applications of curcumin are limited, owing to two major challenges: poor aqueous solubility and lack of bioavailability. In this regard, biosurfactant-based micellar systems have surged recently for the development of novel and more effective formulations because of their biological relev...

Studies on oxidative stress on organisms especially on invertebrates are widely used as biomarkers in relation to the redox regulatory system to assess the freshwater environment. Despite broad availability of Indian apple snail Pila globosa, no data on its antioxidant and oxidative stress status are available. Therefore, determination of tissue-sp...

Oxidative stress (OS), a clinical predicament characterized by a shift in homeostatic imbalance among prooxidant molecules embracing reactive oxygen species (ROS) and reactive nitrogen species (RNS), along with antioxidant defenses, has been established to play an indispensable part in the pathophysiology of subfertility in both human males and fem...

Multiple cellular processes are regulated by oxygen radicals or reactive oxygen species (ROS) where they play crucial roles as primary or secondary messengers, particularly during cell proliferation, differentiation, and apoptosis. Embryogenesis and organogenesis encompass all these processes; therefore, their role during these crucial life events...

The semi-sessile, ectothermic, amphibious mollusc Pila globosa receives less attention for studying its environmental aspects. Its ecological role, ecotoxic and physiological responses to environmental factors including pollution and during dormant periods such as hibernation and estivation to understand key information about many cellular metaboli...

Objectives Widely used organophosphorus pesticides, methyl parathion (MePa), alter the reproductive functions in various animals and humans by induction of oxidative stress on augmented release of reactive oxygen species (ROS). MePa affects semen quality by inducing DNA damage through spermatogenic stages. Several heat shock proteins (HSPs) are exp...

Studies on oxidative stress physiology on molluscs to monitor the aquatic environment, influenced by pollutants and stressors are very limited in general and in Indian apple snails, P. globosa in particular. The main focus of the present study was to establish the baseline data on a redox regulatory system in P. globosa sampled across the Indian su...

Bi-directional crosstalk between Ca²⁺ signaling and ROS modulates physiological processes as a part of a regulatory circuit including sperm function. The role of transient receptor potential vanilloid 1 (TRPV1) in this regard cannot be undermined. This is the first report demonstrating the Ca²⁺-sensitive TRPV1 channel to be under-expressed in sperm...

Information on the oxidative stress physiology parameters (OSPP) in general and as a function of the fluctuation of Mg, Ca and organic carbon present in soil and aquatic physico-chemical factors such as pH, temperature and salinity in particular are scanty in the amphibious snail Pila globosa. A spatio-temporal analysis of redox metabolism (as OSPP...

Problem: Recurrent Pregnancy Loss (RPL) is usually evaluated from a women's perspective, however, recent evidence implies involvement of male factors as paternally expressed genes predominate placenta. During fertilization, prior to implantation the immune system purposefully produces early pregnancy factors with potent immunomodulatory properties...

Climate change and temperature variations are of paramount importance to aquaculture. Here, we investigated the thermal stress response of five life stages of tilapia viz. spawn, fry, fingerling, juveniles and adults exposed to different temperatures (28°C to 40°C). Stress response was assessed in terms of survival/mortality, thermal shock, changes...

The end product of germ cell maturation is the spermatozoa that are produced through a definite pattern in nature that requires a special environment. During the proliferation of diploid germ cells, the spermatogonia repopulate the testis lobules by mitotic cell division. The natural germ cell proliferation and maturation has been modulated and man...

The present work assessed the seasonal changes in germ cells (GC) and the maturation of rohu, Labeo rohita and tilapia, Oreochromis niloticus. Here, we showed two species representing two diverse types of spawning behavior, having two distinct GC proliferation and spawning patterns. During July, a single peak spawning season for rohu was recorded w...

Oxidative stress (OS) is implicated in 80% of idiopathic male infertility (IMI) patients where exposure to redox active environmental toxicants such as polyaromatic hydrocarbons (PAHs) may play. In the present study the seminal exposome of various PAH was analyzed in two separate cohorts including 43 fertile donors and 60 IMI patients by HPLC and r...

Biomimetic hydrogels composed of natural polysaccharides have invariably blossomed as niche biomaterials in tissue engineering applications. The prospects of creating an extracellular matrix (ECM)-like milieu from such hydrogels has garnered considerable importance. In this study, we have fabricated bioscaffolds comprising dialdehyde alginate and x...

American Center for Reproductive Medicine Reasearch Collaborators-2018

Heat shock protein A2 (HSPA2) is a testis-specific molecular chaperone of the 70 kDa heat shock protein (HSP70) family and reported to play a key role in spermatogenesis as well as in the remodelling of the sperm surface during capacitation. It is established that mice lacking HSPA2 gene are infertile and spermatozoa that fail to interact with the...

A positive association between oxidative stress and hyper-thyroid conditions is well established. Vitamin E (VIT-E) and curcumin (CRM) are considered as potent antioxidant small molecules. Nuclear factor erythroid 2–related factor 2(NRF-2) is known to bind with antioxidant response element and subsequently activate expression of antioxidant enzymes...

Apple snails are one of the most survived fresh water invasive species that causes massive economic loss to vegetations, especially to the rice fields. They survive against many environmental stressors and are considered as a major pest in water-lodged cultivated area due to their intrusiveness, adaptation mechanisms and survivability. Therefore, t...

Cyclodextrin glycosyltransferase (CGTase; EC 2.4.1.19) is an extracellular enzyme which converts starch into nonreducing, cyclic malto-oligosaccharides called cyclodextrins (CDs). It is a hydrolytic enzyme that carries out reversible intermolecular as well as intramolecular transglycosylation reaction towards cyclization, coupling, and disproportio...

The present disclosure relates to a process for developing seaweed extract and composition useful against UV-radiation, oxidative damage and skin cancer.

The present disclosure relates to a method to detect paternal contributors present in seminal extracellular vesicles (SEVs) in case of recurrent pregnancy loss.

As global population escalates; the need for better healthcare shall inevitably intensify. Current scenario, thus, calls for a versatile technology for the construction for smart biomaterials to cater to the needs. In this context; hydrogels comprising carboxymethyl chitosan and polyvinyl alcohol (CMCS/ PVA) were fabricated using a green crosslinke...

Recent evidence entail paternal factors as plausible contributors in spontaneous recurrent pregnancy loss (RPL). Seminal extracellular vesicles secreted from cells of male reproductive tract carry regulatory proteins and RNAs. They are proposed to regulate sperm maturation and function while their fusion to endometrial stromal cells helps in decidu...

Diet has important effects on normal physiology and the potential deleterious effects of high fat diets and obesity on male reproductive health are being increasingly described. We conducted a histological review of the effects of chronic high fat (HF) diet (using a mouse model fed a 45% fat diet for 21 weeks) with a discovery proteomic study to as...

A series of C3‐symmetricaltriazine‐cored small dendritic molecules containing three to nine peripheral o‐carborane clusters were synthesized through Cu(I)‐catalyzedazide–alkyne cycloaddition reactions. The newly synthesized molecules containing multiple o‐carborane moieties were characterized using nuclear magnetic resonance and matrix‐assisted las...

Sperm proteins undergo post-translational modifications, such as phosphorylation, acetylation, and ubiquitination, which in turn play a key role in determining their fertilizing ability. In the current study, we examined the sperm proteome of men with unilateral and bilateral varicocele to identify the key proteins affected by acetylation to gain a...

HSPA2, a testis-specific protein belonging to the HSP70 family, is ubiquitously found in the testes and spermatozoa. It plays a pivotal role in the spermatogenesis process, and associated with human sperm-egg recognition. During various stress conditions, ligand-receptor binding between extracellular HSP70 and TLR 4 has been well established in man...

Background: This article describes the research trends in sperm DNA fragmentation (SDF) over the past 20 years (1999-2018) using a scientometric approach. Methods: A stepwise approach was adopted to retrieve scientometric data (articles per year, authors, affiliations, journals, countries) from Scopus and analyze the publication pattern of SDF w...

Recurrent pregnancy loss (RPL) is a perplexing problem experienced with two or more consecutive unexplained miscarriages wherein the cause remains unexplained in >50% of cases. However, despite several evidences of involvement of paternal factors on early embryogenesis and placental development, its contribution towards RPL has been largely unexplo...

Aims: To understand the molecular pathways involved in oxidative stress (OS)-mediated sperm dysfunction against a hypoxic and hyperthermic microenvironment backdrop of varicocele through a proteomic approach. Results: Protein selection (261) based on their role in redox homeostasis and/or oxidative/ hyperthermic/ hypoxic stress response from the...

Antioxidant supplementation in idiopathic male infertility has a beneficial effect on semen parameters. However, the molecular mechanism behind this effect has not been reported. The objective of this study was to evaluate the sperm proteome of idiopathic infertile men pre- and post-antioxidant supplementation. Idiopathic infertile men were provide...

Despite advances in the field of male reproductive health, idiopathic male infertility, in which a man has altered semen characteristics without an identifiable cause and there is no female factor infertility, remains a challenging condition to diagnose and manage. Increasing evidence suggests that oxidative stress (OS) plays an independent role in...

The present study was aimed at evaluating the antidiabetic activity of the ethanol bark extract of a mangrove plant Avicennia officinalis (AOEB) in streptozotocin (STZ)-induced diabetic mice. The study revealed AOEB could lower the blood glucose level significantly (p < .05) in diabetic mice at dose of 200 mg/kg b.w. The elevated total cholesterol,...

Semen contains leucocytes and round cells, besides spermatozoa. The objective of this study was to identify whether the proteins from round cells and leucocytes affect the proteomic analysis of spermatozoa. Cryopreserved human sperm samples were divided into four groups: (1) samples with ≥1 × 10^6/ml leucocytes unprocessed; (2) samples with ≥1 × 10...

Oxidative stress is implicated in both hypo- and hyper-thyroid conditions. In the present study an attempt has been made to elucidate possible interaction between vitamin E or/and curcumin (two established antioxidants) with active portion (redox signaling intervening region) of nuclear factor erythroid 2-related factor 2 (NRF2) as a mechanism to a...

Purpose: To evaluate the in vitro impact of ritalinic acid (RA), a major metabolite of methylphenidate (drug to treat attention-deficit hyperactivity disorder), on sperm motility, vitality and oxidative stress. Materials and methods: Semen samples (n=13) were collected from healthy donors and a semen analysis was performed according to World Hea...

Despite advances in the field of male reproductive health, idiopathic male infertility, in which a man has altered semen characteristics without an identifiable cause and there is no female factor infertility, remains a challenging condition to diag- nose and manage. Increasing evidence suggests that oxidative stress (OS) plays an independent role...

Xylocarpus granatum is a medicinal mangrove plant, traditionally used for the treatment of diarrhoea, cholera, fever, dyslipidaemia, inflammation, etc. The present study was aimed to evaluate the in vitro antidiabetic ( α -glucosidase inhibition assay) and antioxidant (ABTS scavenging and metal chelating assay) activities of ethanol, methanol, and...

The present study was designed to identify antidiabetic and antioxidant constituents from ethanol bark extract of a medicinally important mangrove plant Xylocarpus granatum J. Koenig, using in vitro bioactivity-guided fractionation. The repeated fractionation of X. granatum ethanol bark extract (XGEB) by silica gel column chromatography yielded a c...

Purpose: Aberrant expression of seminal plasma proteins are associated with altered homeostasis that may affect the fertilizing ability of spermatozoa. However, the precise roles of seminal exosomes on sperm function remain unclear. The objective of this study was to identify the differentially expressed proteins (DEPs) associated with varicocele-...

With the current scenario of green chemistry looming large, biocomposites comprising cellulose and polyvinyl alcohol were synthesized using a non-toxic crosslinker, tetraethyl orthosilicate. Surface modification of the aforementioned composites was performed by non-thermal argon plasma. The plasma treatment rendered the composites hydrophilic; evid...

In sperm proteomic experiments round cells and leukocyte proteins are profiled along with sperm proteome. The influence of round cell and leukocyte proteins on the sperm proteome has not been investigated. The objective of this study was to identify if the proteins from round cells, including leukocytes, interfere with the proteomic analysis of spe...

Elevated levels of reactive oxygen species (ROS) are a major cause of male infertility. However, some men with high seminal ROS levels are still fertile. The main objective of this study was to understand the molecular mechanism(s) responsible for the preservation of fertility in those men. Semen samples from fertile men were divided into two group...

Purpose: To identify the paternal factors responsible for aberrant embryo development leading to loss of foetus in recurrent pregnancy loss (RPL) through proteomic analysis of ejaculated spermatozoa. Materials and methods: This prospective study consisted of male partners of RPL patients (n=16) experienced with two or more consecutive unexplaine...

In human sperm proteomic experiments, leukocyte and round cell proteins may contaminate the sperm proteome and affects the bioinformatic results. The main objective of this study was to identify the possible interference of these proteins, especially from leukocytes, in identification of sperm functional pathways through proteomic and bioinformatic...

Infertility affects nearly 15 per cent of all couples within the reproductive age worldwide, with about 50 per cent being exhibited in the male, called male factor infertility. Successful reproduction is dependent on sperm chromatin integrity. Spermatozoa are highly specialized cells that aim to transmit the paternal genomic blueprint to the oocyte...

Cyclodextrin glycosyl transferases (CGTases) are produced by a wide range of bacteria, mainly Bacillus species by submerged fermentation. CGTase production is highly dependent on the strain, medium composition and culture conditions. The present investigation was undertaken with an objective to screen, isolate, identify the microorganism producing...

Dysfunctional sperm maturation is the primary reason for the poor sperm motility and morphology in infertile men. Spermatozoa from infertile men were fractioned on three-layer density gradient (80%, 60%, and 40%). Fraction 1 (F1) refers to the least mature stage having the lowest density, whereas the fraction 4 (F4) includes the most dense and morp...

Varicocele is the most common treatable cause of male infertility. Molecular changes associated with defective spermatozoa are predominantly due to alterations in the expression levels of their proteins. In varicocele patients, pathophysiological state, compromised testicular function and epididymal dysfunction may change the composition of the sem...

Male infertility is a global health concern because it affects not only the physical well-being of individual patients, but also the psychological health of couples who want to have children. It also has significant negative societal implications. Incertain societies, for a man to not be able to father children means that he will suffer from ridicu...