Luke ConnollyUniversity of Plymouth | UoP · School of Health Professions
Luke Connolly
PhD
About
23
Publications
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Publications
Publications (23)
Background
High‐intensity interval training (HIIT) is feasible and beneficial for some people with Parkinson's (PwP), although adherence to extended programs may be problematic. PwP face barriers to exercise such as lack of time, expense, and difficulty with travel logistics due to motor symptoms. HIIT based in the home setting if practical, could...
High-intensity interval training (HIIT) is useful and feasible for some people with Parkinson’s (PwP), although long-term adherence may be problematic. If practical, undertaking HIIT in the home setting could be a way to encourage continued participation. However, no home-based HIIT programme has been developed for this population. Therefore, the o...
Background:
Exercise is important for people with Parkinson's (PwP), with high-intensity interval training (HIIT) proposed as a feasible and effective exercise modality. However, no literature synthesis for PwP has been undertaken.
Objectives:
To evaluate the feasibility, safety, physiological and clinical effects of HIIT for PwP.
Methods:
Sys...
Background: Perceptual responses (affect, enjoyment and perceived exertion) and exercise performance (running speed) during self-paced continuous running are dependent on environmental setting (treadmill vs overground), but these observations remain unclear during interval exercise. We examined the running speed, affect, enjoyment, and perceived ex...
UNSTRUCTURED
These are author responses to peer review.
BACKGROUND
Understanding and assessing patients’ body movements is essential for physical rehabilitation but is challenging in video-consultations as clinicians are frequently unable to see the whole patient or see the patient performing specific movements.
OBJECTIVE
The objective of this exploratory study was to assess readily available technolog...
Background
Understanding and assessing patients’ body movements is essential for physical rehabilitation but is challenging in video consultations, as clinicians are frequently unable to see the whole patient or observe the patient as they perform specific movements.
Objective
The objective of this exploratory study was to assess the use of readil...
Objective:
The purpose of this systematic review was to investigate whether aerobic training (AT) or resistance training (RT) is most effective in terms of improving lower limb physical function and perceived fatigue in persons with multiple sclerosis (pwMS).
Data sources:
Nine databases (MEDLINE, EMBASE, CINAHL, AMED, PEDro, SPORTdiscus, PsycIN...
Background
: The short-term benefits of exercise in people with multiple sclerosis (MS) are well established. To sustain benefits exercise needs to continue long-term. Despite important clinical implications, no systematic reviews have synthesized evidence on adherence and drop-out in MS exercise interventions.
Objectives
: 1) To summarize reporte...
![Blood [glucose] response following the oral glucose tolerance test OGTT...](publication/340035078/figure/fig4/AS:960132930367502@1605924937623/Blood-glucose-response-following-the-oral-glucose-tolerance-test-OGTT-displayed-over_Q320.jpg)
![[High-density lipoprotein (HDL) cholesterol] before and after 12 weeks...](publication/340035078/figure/fig5/AS:960132930338839@1605924937956/High-density-lipoprotein-HDL-cholesterol-before-and-after-12weeks-of-home-based_Q320.jpg)
PurposeThis study tested the hypothesis that a novel, audio-visual-directed, home-based exercise training intervention would be effective at improving cardiometabolic health and mental well-being in inactive premenopausal women.Methods
Twenty-four inactive premenopausal women (39 ± 10 years) were randomly assigned to an audio-visual-directed exerci...
We examined the effects of 16 weeks of football training and dietary advice on blood glucose control and health status in 55‐ to 70‐year‐old women and men with prediabetes. Fifty participants with prediabetes (age; 61 ± 6 years, BMI; 29.6 ± 4.7; VO2max 22.3 ± 5.7 mL·min⁻¹·kg⁻¹) were randomized into a football and dietary advice group (F+D; n = 27)...
A diet rich in vegetables is known to provide cardioprotection. However, it is unclear how the consumption of different vegetables might interact to influence vascular health. This study tested the hypothesis that nitrate-rich vegetable consumption would lower systolic blood pressure but that this effect would be abolished when nitrate-rich and thi...
![Blood [glucose] response during the oral glucose tolerance test (OGTT)...](publication/319941392/figure/fig3/AS:960131667853323@1605924636507/Blood-glucose-response-during-the-oral-glucose-tolerance-test-OGTT-displayed-over_Q320.jpg)
Purpose:
To compare the effects of self-paced high-intensity interval and continuous cycle training on health markers in premenopausal women.
Methods:
Forty-five inactive females were randomised to a high-intensity interval training (HIIT; n = 15), continuous training (CT; n = 15) or an inactive control (CON; n = 15) group. HIIT performed 5 × 5...
The health benefits of playing football and the importance of exercise and social contact for healthy ageing are well established, but few older adults in the UK take enough exercise. Football is popular, flexible in format and draws players into engrossing, effortful and social exercise, but the physical demands of play at full speed may make it u...
![Figure 1: Plasma [glucose] response following the OGTT displayed over...](profile/Luke-Connolly-2/publication/305719133/figure/fig2/AS:391009558122514@1470235351626/Plasma-glucose-response-following-the-OGTT-displayed-over-time-in-HIT-LIT-and-CON_Q320.jpg)
![Figure 2: Plasma [insulin] response following the OGTT displayed over...](profile/Luke-Connolly-2/publication/305719133/figure/fig3/AS:391058690199565@1470247065609/Plasma-insulin-response-following-the-OGTT-displayed-over-time-in-HIT-LIT-and-CON_Q320.jpg)
![Figure 4: Fasting plasma [sICAM-1] (a) and [sVCAM-1] (b) in HIT, LIT...](profile/Luke-Connolly-2/publication/305719133/figure/fig1/AS:389992221298689@1469992799053/Fasting-plasma-sICAM-1-a-and-sVCAM-1-b-in-HIT-LIT-and-CON-before-and-after-the_Q320.jpg)
Purpose:
We tested the hypothesis that low-volume high-intensity swimming has a larger impact on insulin sensitivity and glucose control than high-volume low-intensity swimming in inactive premenopausal women with mild hypertension.
Methods:
Sixty-two untrained premenopausal women were randomised to an inactive control (n = 20; CON), a high-inte...
We aimed to study whether short-duration vibration exercise or football sessions of two different durations acutely changed plasma markers of bone turnover and muscle strain. Inactive premenopausal women ( n=56 ) were randomized to complete a single bout of short (FG15) or long duration (FG60) small sided football or low magnitude whole body vibrat...
The hypothesis that the adaptive capacity is higher in human upper- than lower-body skeletal muscle was tested. Furthermore, the hypothesis that more pronounced adaptations in upper-body musculature can be achieved by 'low-volume high-intensity' as compared to 'high-volume low-intensity' exercise training was evaluated. A group of sedentary premeno...
Purpose
The present study investigated the effects of 16 weeks of small-volume, small-sided soccer training (soccer group (SG) n = 13) and oscillating whole-body vibration training (vibration group (VG) n = 17) on body composition, aerobic fitness and muscle PCr kinetics in healthy inactive premenopausal women in comparison with an inactive control...
To examine the physical capacity and physiological response to the Yo-Yo Intermittent Endurance level 2 test (IE2) for untrained individuals and trained male soccer players, and to investigate the determinants of intense intermittent exercise performance.
Thirty-four healthy untrained males (UTR) and fifteen age-matched trained soccer players (TR)...
Questions
Question (1)
I have been using Biorad equipment and my target proteins are CS, PFK and FXYD1.
The contents of the sample buffer (which we have used many times with no problems) are Tris Base, DTT, SDS, Glycerol and Bromphenol blue.
The gel we are using is Bio-Rad Criterion TGX Stain-Free Precast Gel 4-15%.
The transfer buffer we prepare includes Tris-base, glycin, SDS, ethanol and DDH2O (we have used two different batches of this transfer buffer with the same smeared results.
The running phase seems to work normally and the process terminated before the blue dye reaches the bottom of the gel.
As we are working with many samples and running/transferring 5 gels/membranes at one time, we have cut the gels so CS, PFK and FXYD1 samples are on the same membrane when transferred. We use two transfer machines connected to a Bio-Rad PowerPac Basic Power Supply. It should be noted that an error code appears and displays short circuit error and I am awaiting a response from Bio-Rad with troubleshooting this problem.
However, after the transfer, the markers we use (All Blue and Dual Colour) tend to look smeared and once we have finished blocking and adding antibodies, the pictures provided using the Bio-Rad chemidoc look even worse (please find example for PFK attached).
If the sample buffer is made incorrectly (e.g. too much Tris Base used in relation to other contents), how much of an effect could this have on the results?
