Łukasz Paprotny

• Doctor of Pharmacy
• Head of Research and Development Center at SHIM-POL

Currently, there is a significant demand for natural biologically active compounds. Emphasis is placed on improving the quality and safety of processed natural products, which is understandable in light of the frequently observed instability of natural compounds and their degradation, among others, to compounds of unknown biological activity. In th...

The health-promoting properties of vitamin K stimulate the growing interest in this compound, which translates into the development of new analytical methodologies for its determination. New, more efficient methods of its isolation are sought, paying increasingly more attention to the methods within currently available extraction techniques that, o...

Vitamin K, as a natural protector of our blood, bones, kidneys, and brain, is essential for human health. It is also considered an effective anti-aging agent with comprehensive biological effects, including antifungal, antibacterial, anti-inflammatory, analgesic, and even antioxidant properties. Of these, the least is known about the antioxidant pr...

The extensive use of rodenticides poses a severe threat to non-target species, particularly birds of prey and scavengers. In this study, a GC-MS/MS-based method was used to unlock the cause of bird deaths in Poland. Organs (liver, heart, kidney, and lungs) collected during autopsies of two rooks (Corvus frugilegus) and one carrion crow (Corvus coro...

One of the most important issues considered in ecotoxicology is the elevated accumulation of different elements in soil as a consequence of anthropogenic activities. In this paper, determination of nine elements in soil obtained from three different forest complexes (sycamore, beech, and spruce) in urbanized and non-urbanized regions of Tatra Natio...

Biological properties of menaquinone-7, one of the vitamin K2 vitamers (K2MK-7), both those proven and those that remain to be investigated, arouse extensive interest that goes beyond the strictly scientific framework. The most important of them is the prevention of age-related diseases, considering that we live in the times identified as the era o...

6-Thioguanine is an immunosuppressive drug, an analogue of guanine, applied to treat acute leukemia and inflammatory bowel disease. Excessive use of 6-thioguanine during clinical treatment may cause side effects. Moreover, providing a dose too low will be ineffective. Therefore, there is a critical need for a rapid, selective and routine approach t...

In this article, we have presented the development and validation of a rapid and sensitive reversed-phase liquid chromatography with tandem mass spectrometry (LC-MS/MS) method for the determination of vincristine (VCR) in patient serum samples. Chromatographic separation was achieved on a Kinetex® (Singapore) column using a mobile phase consisting...

We compared the antioxidant activity of serum and plasma samples of a known glutathione content with the activity of glutathione, whilst determining to what extent various stress factors might change the activity of the tested samples. Copper ions and benzene were used as examples of environmental stress factors, and xenobiotics in the form of repr...

The imbalance between the production of Reactive Oxygen Species (ROS) and their sequestration promotes the formation of so-called oxidative stress conditions which are considered crucial in the aging process and development of many human diseases. Glutathione plays an essential role in the antioxidative barricade against ROS. Its role in the detoxi...

Sulthiame is an old antiepileptic medicine with controversial history, which effectiveness and safety in use were stated in some current studies. However, there is still a need of further clinical examinations for confirmation its usefulness and tolerability in monotherapy and add‐on therapy for epilepsy of various etiologies. A fully‐validated RP...

Przedmiotem badań były próby przerośniętych migdałków gardłowych usuniętych na drodze adenotomii od 56 dzieci, w tym 30 chłopców i 26 dziewczynek, narażonych i nienarażonych na bierne palenie, mieszkających na obszarze administracyjnym Chorzowa. W pracy przedstawiono charakterystyką statystyczną występowania Fe i Mn oraz przeprowadzono badania nad...

We compared results of vitamin D levels from 41 individual patients, paying additional attention to two groups of results (insufficient and toxic levels). Results were classified into two groups: those with vitamin D concentration of less than 30 ng/mL (according Roche method) and those with concentration greater than 100 ng/mL. These analyzes were...

Introduction: Effective diagnosis of cardiovascular diseases requires the right tools to be used enabling selective and sensitive analysis of their biomarkers. One of them is homocysteine (Hcy), nowadays determined by immunoassays and chromatographic methods. This study aims to compare the results obtained by direct chemiluminescence immunoassay (...

Dysbiosis is a disorder of the bacterial flora of the human digestive tract. It is usually diagnosed clinically by direct detection of an abnormal pattern of the intestinal microbiota. The intermediate diagnosis based on determining the content of microflora metabolites, considered as chemical markers of this disorder, is still rarely used. This is...

The objective of the research was to determine the effect of habitat type of selected species of trees on the nature of cooccurrence of Fe, Mn, Zn, Cu, Pb, Cd, Ni, Cr and Co. The presence of speciation forms of these metals was investigated, with reference to the species composition of tree stands in selected areas of the Tatra Mountain National Pa...

1α,25(OH)2D3 is metabolized through the oxidation pathway of B-ring side chain at C-24 into inactive calcitroic acid or at C-23 leading to formation of 1α,25(OH)2D3-26, 23-lactone. In the late 90-ties another metabolic pathway has been described – modification of hydroxyl groups configuration of A-ring at C-3 with formation of 3-epimers of 25(OH)D3...

Introduction: In previous paper we confirmed the presence of microbiological, inorganic and organic contaminations in home-made poppy straw extracts - ‘compote’. These contaminations may influence on its toxicity, however there is no appropriate experimental studies. The authors decided to develop method of ‘compote’ purification and determine and...

Our research investigated the bioavailability of Pb, Cd, and Ni in the soil of Tatra Mountain National Park (Chochołowska Valley, Kościeliska Valley, Małej Łaki Valley, and Strażyska Valley). The content of Pb, Cd, and Ni was estimated using the ASA method with accuracy of 0.1 μg/g. Rudd's methodology was applied to estimate direct and indirect bio...

The subject of the research were samples of overgrown adenoids removed by adenoidectomy 56 children, including 30 boys and 26 girls, exposure and unexposure from passive smoking, living in the administrative area of Chorzów. The statistic characteristic of Fe and Mn occurrence is presented in the thesis. The studies were carried out on the changes...