Luiz Antonio Biasi

Universidade Federal do Paraná | UFPR · Departamento de Fitotecnia e Fitossanidade

Viticulture in Brazil develops from the south to the north of the country. Due to the high edapho-climatic variability, Brazilian viticulture is divided into 3 main productive locations: temperate viticulture in south, subtropical viticulture in southeast and tropical viticulture in north/northeast. Temperate viticulture in southern Brazil is the m...

Grapes are one of the most important fruit commodities, as economic plants, with good agricultural characteristics being also an important source of antioxidants, such as polyphenols. These compounds confer several benefits to human health, performing biochemical and pharmacological effects such as anticancer, anti-inflammatory, antimicrobial, and...

The objective of this study was to evaluate the effective temperature for overcoming the dormancy of ‘Fuyu’ persimmon tree buds. Stem samples were collected three times between 2013 and 2014. Stems were maintained in a climate incubator chamber at 3, 6, 9, and 12 °C. For each temperature, five numbers of additional chilling hours (CH) (0, 240, 384,...

The nutritional state of cultivated plants can be influenced, due to the poultry litter doses and basalt powder, thus affecting its proper growth. This work aimed to evaluate the nutritional efficiency of pear, cultivated in different poultry litter doses and basalt powder. The experiment was conducted in the orchard of the fruit growing sector of...

The number of organic vineyards around the metropolitan region of Curitiba-Paraná-Brazil is increasing, and alternative products to control downy mildew (Plasmopara viticola) and grapevine leaf spot (Pseudocercospora vitis) are crucial for the development of organic grape production. The objective of this study was to compare the efficacy of ten al...

This study identified physiological and biochemical changes in ‘Fuyu’ persimmon buds during dormancy. Branches were collected between March and August 2015. Dormancy was evaluated by biological testing of isolated node cuttings at 25 °C and a photoperiod of 16 h. The variables analyzed were water content; reducing sugar content; respiratory activit...

Blueberries have large amounts of antioxidant and anti-inflammatory compounds, playing an important role in improving health. In vitro culture is a possible method for vegetative propagation overcoming the problems of pathogen infection. Most studies on the in vitro culture of blueberries focus on highbush and lowbush cultivars. However, blueberry...

Because this species Rubus brasiliensis Mart. is not commercially cultivated as an agricultural crop, there is significant lack of information on cultivation practices, particularly about propagation. The present study was carried out to evaluate rooting of stem cuttings harvested at different dates and subsequently treated with indole-3-butyric ac...

Abstract Blueberry is an important fruit crop, with many health benefits. Despite its importance, much remains to be studied concerning the dormancy dynamics in rabbiteye cultivars growing in a mild winter climate. In this research, the dormancy in blueberry, rabbiteye cultivars ‘Bluegem’, ‘Climax’, ‘Delite’, and ‘Powderblue’, was studied in a mild...

Vineyards of ‘Niagara Rosada’ have shown great productive potential. However, the grape production has been affected by factors related to climate adversities. Thus, this study aimed to evaluate the effect of plastic cover and bagging of bunches of ‘Niagara Rosada’, in Almirante Tamandaré, PR, Brazil. Plastic cover used was of polyethylene with 250...

A miniestaquia tem sido utilizada para diversas espécies lenhosas, apresentando maior eficiência do que a propagação por estaquia convencional, devido seu elevado rendimento de material propagativo e maior porcentagem de enraizamento das miniestacas. Este trabalho foi realizado para avaliar a eficiência da técnica de miniestaquia para amoreira pret...

Blueberries are valued for their taste and their high nutritional benefits, including their antioxidant and anti-inflammatory properties.In vitroculturing is an alternative method for clonal propagation, and has been usedin many biotechnological studies. Most blueberry research is concentrated on highbush and lowbush taxa (Vac-cinium corymbosumandV...

A produção de mudas in vitro de amoreira-preta possibilita a obtenção rápida e sadia de mudas. O objetivo dessa pesquisa foi otimizar um protocolo de micropropagação da amoreira-preta ‘Xingu’, definindo a concentração mais eficiente de BAP para multiplicação, o melhor agente gelificante a necessidade de AIB no enraizamento ex vitro e a sobrevivênci...

The demand for blackberry has been increasing due to the results of studies on its nutraceutical properties. Moreover, the rusticity of its plants allows its cultivation with reduced use of pesticides, becoming feasible to grow it in areas of environmental protection (APA). As a non-traditional crop in the country and its requirement in specific cl...

Despite the expansion of viticulture in Brazil, the lack of chilling hours is a factor that requires the use of growth inducers to promote sprouting. Erger™ is a product of low toxicity and efficient in the bud induction of apple. However, there is a lack of studies testing such a product on the grapevine. This research was carried out to evaluate...

Antioxidants are compounds with varied chemical structures that are affected by biotic and abiotic factors. The objective of this study was to characterize and compare bioactive compounds and the antioxidant capacity of fruit from four blackberry cultivars produced under different climatic conditions. Ascorbic acid content, total polyphenols, flavo...

Essential oils are broadly used in the food, perfume, and pharmaceutical industries. These are associated with ecological functions of defense and attraction of pollinators, and usually undergo quantitative and qualitative variation in response to the environment. Thus, the aim of this chapter is to highlight the essential oil content, chemical com...

In plant micropropagation, the establishment stage is difficult, due to the presence of microorganisms in tissues from field-grown matrices, especially for bamboo. This study aimed to establish an efficient asepsis protocol for Bambusa oldhamii explants from field plants, as well as to carry out the molecular identification of a possible endophytic...

Micropropagation is an important technique for clonal mass propagation and a tool for in vitro studies. One of the first steps to overcome in this process is the establishment of new explants in vitro. ‘Delite’ rabbiteye blueberry was cultured in vitro with four cytokinins (zeatin (ZEA), 6-(γ-γ-dimethylallylamino)-purine (2iP), 6-benzylaminopurine...

In order to analyze the pathogenicity and antagonism between grapevine rootstocks and soil microorganisms, plant growth-promoting bacteria with antibiotic effect were selected from the phytopathogen Ilyonectria liriodendri, 2T-VSC-101 isolated monosporic, in in vitro antibiosis tests. The bacterial access that produced the largest zone of inhibitio...

In vitro culture is a very important technique in plant mass propagation, as well as an auxiliary tool to germplasm conservation and gene transfer. The present study developed an efficient multiplication protocol for ‘Brazos’ blackberry. Here, its multiplication ability was evaluated using in vitro shoots grown on medium supplemented with different...

Stevia (Stevia rebaudiana Bertoni) is a species characterized by producing non-caloric substances with high sweetening potential. Among these substances, rebaudioside A and stevioside are produced in greater quantity. Plant density and harvesting time are factors that affect biomass and sweetening compounds yield in this species. The objective of t...

O presente trabalho teve por objetivo avaliar o efeito da 6-benzilaminopurina (BAP) na multiplicação de brotações e de diferentes meios de cultura no enraizamento in vitro da amoreira-preta cv. Xavante. O primeiro experimento foi constituído por três concentrações de BAP (0, 1 e 5 ?M) em meio de cultura MS. O segundo experimento foi constituído pel...

This study presents the first micropropagation protocol for greenberry (Rubus erythroclados), a wild Brazilian species with edible green fruits. In the in vitro multiplication stage, three concentrations of benzyladenine (BA) were tested (0, 5 and 10 μM), combined with three concentrations of indolebutyric acid (IBA) (0, 3 and 6 μM) in two subseque...

Studies on adaptation to the cultivation site are necessary for the recommendation of new cultivars. The aim of this study was to evaluate the phenological development, productivity and fruit quality of eight blueberry cultivars from the rabbiteye group (Aliceblue, Bluebelle, Bluegem, Briteblue, Climax, Delite, Powderblue and Woodard) and two from...

This study aimed to evaluate the flowering period and the physicochemical characteristics of peaches, aiming to find those most promising for the diversification of the peach crop in the Metropolitan Region of Curitiba, Paraná State. We evaluated 8 selections and three cultivars in Porto Amazonas and 10 cultivars in Araucaria for flowering period,...

This study presents the first micropropagation protocol for greenberry (Rubus erythroclados), a wild Brazilian species with edible green fruits. In the in vitro multiplication stage, three concentrations of benzyladenine (BA) were tested (0, 5 and 10 μM), combined with three concentrations of indolebutyric acid (IBA) (0, 3 and 6 μM) in two subseque...

Downy mildew (Plasmopara viticola) is among the most important grape disease in the world. Disease control is mostly based on preventive management, including reduction of overwintering inoculum, green pruning, use of less susceptible cultivars, and fungicide applications. The objective of this study was to describe the incidence and severity of P....

RESUMO O mecanismo fisiológico da dormência de gemas de espécies frutíferas temperadas ainda não foi totalmente esclarecido, sendo esse conhecimento importante para se entender melhor o comportamento das plantas em diferentes climas. Este experimento teve por objetivo caracterizar a dinâmica de brotação de gemas de videira cv. Bordô, por meio do te...

The essential oil yield and composition of 17 species of the Asteraceae family were evaluated. Samples were obtained by hydrodistillation of the leaves and inflorescences and they were analyzed by GC-MS and GC-FID. A total of 13 species presented essential oils with yields ranging from 0.06 to 3.36% in fresh samples and from 0.07 to 2.53% in dried...

This work aimed to evaluate the essential oil yield and composition of fresh and dried samples of native tree species in the Atlantic Forest of Parana State, South of Brazil. The essential oil extraction was carried out by hydrodistillation in a graduate Clevenger type apparatus and the chemical composition was analyzed by gas chromatography couple...

Evaluation of yield performance is important to find the most adapted blueberry cultivars in a particular region. This research aimed to evaluate the flowering and hasvesting periods, the production per plant, and fruit quality of eight rabbiteye blueberry cultivars (Aliceblue, Bluebelle, Bluegem, Briteblue, Climax, Delite, Powderblue, and Woodard)...

A micropropagação tem solucionado diversos problemas de propagação de diferentes espécies, porém, as condições in vitro alteram a morfologia das plantas. O objetivo deste trabalho foi caracterizar a morfologia das folhas do porta-enxerto de videira ‘VR043-43’ cultivadas in vitro – normais e in vitro – hiperídricas, aclimatizadas e in vivo. As plant...

O objetivo deste trabalho foi padronizar os estádios de brotação de gemas de ameixeira, caquizeiro, macieira, pereira, pessegueiro, kiwizeiro e videira para o teste biológico de avaliação de dormência. O experimento foi realizado no outono e inverno de 2008. O teste biológico foi conduzido com 40 estacas com 6 cm de comprimento para cada espécie, c...

Muscadine (Vitis rotundifolia Michx.) vines may be male (M), female (F), or hermaphroditic (H). Male flowers have only filaments and anthers, whereas female and hermaphroditic flowers are morphologically perfect. Female flowers are distinguished from hermaphroditic flowers by their reflexed stamens (as opposed to upright) and nonfunctional pollen....

To complete each phase of the growing season, plants must accumulate thermal time at lower base temperature (Tb). Little information exists on Tb variation between either fruit species or cultivars of the same species. We therefore aimed to determine the lower base temperature for contrasting genotypes in precocity of peach, plum, grape, pear, and...

Araucaria angustifolia decreases its growth ratio during the fall and winter in response to adverse environmental conditions such as in temperate fruits where bud dormancy as a survival startegy. The aim of this study was to evaluate the dynamics of the respiratory activity of apical meristems of plagiotropic branches of Araucaria angustifolia . To...

Among the American table grapes, 'Niagara Rosada' stands out as one of the most consumed in Brazil. This is due to its hardiness and adaptation to diverse environmental conditions. Thus, this study aimed to evaluate the effects of plant growth regulators on bunch and berry physico-chemical characteristics in 'Niagara Rosada' grape. The experiment w...

Native muscadines (Vitis rotundifolia Michx.) are dioecious, but muscadine vineyards are usually planted with a mixture of female plants and hermaphroditic pollenizers. Hermaphroditic cultivars are derived from either of two separate sources original hermaphroditic plants, H1 or H2. Nine hermaphroditic and two female cultivars were studied to deter...

The mineral nutrients in vinasse provide support for algal and plant growth. Algal culture releases organic compounds into its liquid culture medium. These organic and inorganic substances can be useful for formulating a plant tissue culture medium, because tissue culture medium is composed of organic and inorganic components. Therefore, the aims o...

Araucaria angustifolia decreases its growth ratio during the fall and winter in response to adverse environmental conditions such as in temperate fruits where bud dormancy as a survival startegy. The aim of this study was to evaluate the dynamics of the respiratory activity of apical meristems of plagiotropic branches of Araucaria angustifolia. To...

Temperate climate fruit trees often exhibit uneven budding and flowering, due to the low number of chilling hours that determine the occurrence of dormancy and its depth. The objective of this study was to determine the depth of bud dormancy in peach tree cultivars and evaluate the efficiency of the tetrazolium test for dormancy and comparing with...

Blackberry has the potential to be grown in subtropical climates, requiring phenological studies for the indication of cultivars adapted to this type of climate. Thus, the aim of this work was to evaluate the phenological behavior of four blackberry cultivars, as well as to determine the physical and chemical characteristics of fruits produced in m...

RESUMO O abacaxi (Ananas comosus L. Merril) é uma planta de propagação vegetativa de grande importância econômica para o Brasil. Entretanto, a dificuldade em se obter mudas sadias, de qualidade, em grande quantidade e de forma rápida é um entrave à expansão desta cultura. Dessa forma, o presente estudo visou estabelecer a regeneração in vitro de ab...

A miniestaquia é uma técnica de propagação vegetativa promissora. Este estudo traz uma contribuição para o avanço do conhecimento na propagação da pitangueira (Eugenia uniflora L.) (Myrtaceae), árvore nativa do Brasil, com potencial para restauração ecológica, exploração pelas indústrias alimentícias, cosméticas e medicinais, e pode melhorar as con...

The minicutting is a promising vegetative propagation technique. This study makes a contribution to the advancement of knowledge in the propagation of Surinam cherry (Eugenia uniflora L.) (Myrtaceae), native tree of Brazil, with potential for ecological restoration, exploitation by food, cosmetic and medical industries, and can improve the life in...

The knowledge of phenological stages and thermal requirement are important in vineyard management because provides information for production and quality of the grapes. This study had the objective to evaluate phenology and thermal demand of the grape cultivars Bordô, Concord, and BRS Carmem on rootstocks Paulsen 1103, IAC 766, and VR 043-43. The e...

Anthracnose is a severe disease present in several grape production areas; it is considered a tropical disease. In South Brazil, high relative humidity and other conditions favor the occurrence of fungal disease in vineyards. The objective of this study was to evaluate the anthracnose incidence progress in grape leaves in three scion cultivars (Con...

Rubus erythroclados is a Brazilian native species with green, sweet fruits that have great potential for fresh market sales and for processing. However, this species is difficult to propagate, and there are no studies about its micropropagation. This study aimed to evaluate the multiplication and rooting of greenberry shoots treated with different...

The aim of this study was to evaluate the viability of the minicutting technique in the vegetative propagation of Surinam cherry (Eugenia uniflora L.), through productivity and survival of ministumps, and rooting of minicuttings originating from grafting and treated with different concentrations of indolebutyric acid (IBA) in the successive collect...

Vinasse is the main pollutant (effluent) obtained from the distillation of sugarcane in the production of fuel alcohol. However, this residue is rich in nutrients that are required by plants. We developed a new culture medium using vinasse for the In vitro propagation of an orchid. The vinasse was treated (decanted and filtered), and the nutrients...

The objective of this work was to evaluate the dormancy dynamic of Imperial Gala apple tree buds and Hosui pear tree buds in a region of low chill occurrence. Experiments were conducted between April and August in 2007 and 2008. Branches were collected every two weeks from an orchard at Porto Amazonas (Parana State, Brazil). On the last sampling da...

O desenvolvimento da videira está bastante relacionado às condições climáticas de cada região; portanto, para se obter frutos de qualidade, torna-se essencial o conhecimento dos estádios fenológicos que, somados ao conhecimento da demanda térmica da planta, fazem com que o viticultor programe as práticas culturais de seu vinhedo reduzindo custos e...

In the present study, the effects of two CaCl2.2H2O levels (440 and 1320 mg L-1) and two subcultures were evaluated on in vitro shoots of Lavandula angustifolia cv. Provence Blue. Ca2+ content of the apical, middle and basal portion of shoots was determined. Increasing CaCl2.2H2O level in the culture medium increased tissue Ca2+ content and decreas...

Ten grapevine cultivars were genotyped at eight microsatellite loci to characterize their identity and genetic diversity. Of these, nine cultivar profiles matched with those of databases and 'Magoon' matched with 'Regale' in the present study and 'Regale' in the University of California (Davis) database, implicating a likely error in planting. The...

Two Vitis labrusca cultivars [Bordô (Ives), Concord] grafted to two rootstocks (1103 Paulsen, VR043-43) were combined in a randomized complete block experiment with a factorialized treatment arrangement in southern Brazil. Clusters were sampled over two seasons (2012, 2013) and analyzed for Brix, titratable acidity (TA) and pH, sucrose, glucose and...

Vegetative development, essential oil yield and composition of nine genetic materials of Lippia alba, linalool chemotype, were compared in the environmental conditions of Southern Brazil. Plants were propagated by stem cuttings and transferred to field in a completely randomized design with four replications and six plants each. The vegetative grow...

O louro (Laurus nobilis L.) é uma espécie de importância econômica, suas folhas são utilizadas na culinária e na extração de óleo essencial. Nas condições do Brasil, a única forma de multiplicar a espécie é via propagação vegetativa, sendo a estaquia considerada uma técnica de grande potencial para utilização. O presente trabalho teve por objetivo...

The aim of this study was to evaluate the effects of fermented extract contend GA3 obtained by Solid State Fermentation and comparing with a commercial source of GA3 (reagent grade) in In vitro culture of common lavender (Lavandula angustifolia Mill.). The residual effects of GA3 also were evaluated in acclimatization. Nodal segments of L. angustif...

Diversos fatores influenciam o processo de aclimatização de plantas micropropagadas, como a concentração de sacarose no meio de cultura e a umidade do ambiente in vitro, que é dependente da técnica utilizada de vedação dos frascos. O objetivo deste trabalho foi estudar o efeito da concentração de sacarose no meio de cultura e a influência causada p...

The aim of this work was to study the influence of sucrose and glucose, amino acids and BAP (6-Benzylaminopurine) levels on in vitro shoot regeneration of fox grape cv. Bordô and grapevine cv. Chardonnay. The nodal segments from micropropagated material were used as explants and half-strength MS medium as the basal medium. Sucrose and glucose at 15...

O enraizamento in vitro aumenta o custo de produção de plantas via micropropagação, pois as plantas necessitam de mais uma fase de desenvolvimento, além do uso de meio de cultura acrescido de reguladores vegetais, sendo que no enraizamento ex vitro há redução das dificuldades associadas à sobrevivência na aclimatização e a redução dos custos de pro...

Piper hispidinervum C.DC. is a plant native to the Amazon region, produces an essential oil rich in safrole. The aim of this study was to evaluate the effect of different methods of drying biomass in chemical composition of the essential oil of P. hispidinervum cultivated in Morretes (PR). The treatments were: fresh leaves; natural drying for 6 and...

Lavandula angustifolia Mill. is an important producer of essential oil, which presents the difficulty of propagation stem cuttings. In this work the propagation of plant was carried out by micropropagation technique, in which certain conditions were evaluated in vitro. The micropropagated plants were grown in São Joaquim (SC). The essential oil of...

The objective of this work was to evaluate the effects of the application of concentrations of indolebutyric acid (IBA) and of bottom heat on rooting of stem cuttings of the blueberry cultivars Bluebelle, Bluegem, and Powderblue in the four seasons of the year. A completely randomized design was used, with a 4x5x2 factorial arrangement (seasons x c...

O substrato deve promover o crescimento das plantas, sendo um componente importante durante a aclimatação das plantas micropropagadas. O objetivo desta pesquisa foi avaliar os efeitos de diferentes substratos durante a aclimatação da uva de raposa cv. Bordô. O experimento foi realizado em casa de vegetação nos meses de fevereiro e março de 2009. Ci...

The objective of this work was to evaluate phenology and yield of peach cultivars in Lapa municipality, state of Paraná, Brazil. Eleven cultivars were evaluated in the 2008/2009, 2009/2010 and 2010/2011 harvest seasons: Aurora-1, Chimarrita, Chiripá, Coral, Eldorado, BRS Granada, BRS Leonense, Maciel, Marli, Premier, and BRS Vanguarda. A randomized...

The Melaleuca alternifolia is a Myrtaceae tree of great medicinal importance. Its essential oil is widely used both in human and in veterinary medicine, having great antiseptic power. It is a difficult plant in the process of rooting cuttings. This study aimed to evaluate the efficacy of different concentrations of NAA and IBA on the rooting of M....

The objective of the research was to determine an index of dormancy (ID) of temperate climate fruit trees buds for evaluation of the biological test of single node cuttings. The biological tests had been done from April to September of 2007 and 2008 with plum, peach, persimmon, kiwi and grape trees. Based in the values of average time for budburst...

Nidularium procerum and Nidularium innocentii belong to the family Bromeliaceae and have ornamental and medicinal characteristics. A procedure for the micropropagation for these bromeliads is described. Seedling explants were cultured on MS media (Murashige and Skoog, 1962), liquid and solid supplemented with 0, 2, 4 and 8μM BAP (6-benzylaminopurin...

The objective of this work was to observe the dormancy dynamics of 'Niagara Branca' grape and 'Bruno' kiwifruit buds in a region of low chill occurrence. Stems were collected weekly during autumn-winter 2009. Extra sets of stems were collected during the last week of winter and maintained at 4-7°C for 672 and 1440 h, for grape and kiwifruit, respec...

The objective of this work was to evaluate the endodormancy of 'Imperial Gala' apple and 'Hosui' pear buds in a region of low chill occurrence in Brazil. Stems were collected weekly during autumn-winter 2009. Extra sets of stems were collected during the last week of winter and maintained at 4-7°C for 1440 h. The evaluation of dormancy was performe...

Lavandula angustifolia Miller (lavender), considered a hard-to-root species, is comprised of many varieties and horticultural forms, and genotypes from different provenances may present variance in rooting capacity. The objective of this experiment was to test different concentrations of indol-3-butyric acid (IBA) on rooting of eight (G1 to G8) L....

The cultivation of calli and cell suspension lines of aromatic plants is important because they are considered to be factories of active compounds such as, for instance, the rosmarinic acid found in Lavandula species. L. angustifolia is considered the most important species because it is regarded to be practically free from camphor, differently fro...

The substrate must promote the growth of the plants, being an important component during the acclimatization of micropropagated plants. The aim of this research was to evaluate the effects of different substrates during the acclimatization of fox grape cv. Bordô. The experiment was carried out at the greenhouse during February and March of 2009. Fi...