Luísa Gouveia

Luísa Gouveia
Laboratório Nacional de Energia e Geologia | LNEG · Unidade de Bioenergia

PhD in Biotechnology

About

134
Publications
70,080
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7,933
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Additional affiliations
January 1987 - October 2015
Laboratório Nacional de Energia e Geologia
Position
  • Researcher

Publications

Publications (134)
Article
The use of microalgae to remediate raw effluent from brown crab aquaculture was evaluated by performing batch mode growth tests using separately the microalgae Chlorella vulgaris (Cv), Scenedesmus obliquus (Sc), Isochrysis galbana (Ig), Nannocloropsis salina (Ns), and Spirulina major (Sp). Removal efficiencies in batch growth were 100% for total ni...
Article
The aim of this work was to study an integrated approach for landfill leachate remediation comprising chemical precipitation with biomass bottom ash as a pre-treatment to reduce color and turbidity followed by bioremediation through microalgae treatment for effluent disposal. Optimal pre-treatment conditions were determined through batch experiment...
Article
Full-text available
Recently, yeast and microalgae mixed cultures have been widely used in biological effluent treatments and biofuel production because such cultures show many advantages over pure cultures. However, industrial effluents often contain toxic compounds; therefore, it is important to evaluate the cell stress response when growing in such conditions durin...
Conference Paper
Wastewater treatment was performed with different microalgae/cyanobacteria strains: Synechochystis sp., Scenedesmus obliquus, Chlorella protothecoides, Chlorella vulgaris, etc. The potential of the produced biomass (at 0.5g/L) was evaluated for several seeds (cucumber, barley, wheat, soya, watercress, and tomato) as a biostimulant for seed germina...
Article
Full-text available
The potential of Scenedesmus obliquus biomass, after treating several wastewaters (WWs) (urban, dairy and brewery industries, cattle and poultry breeding), was evaluated for the production of bio-char, bio-oil and bio-gas, through a pyrolysis process. The experiments were carried out in a fixed bed reactor at a temperature of 748 K during 15 min. T...
Article
The forecasted growth of the aquaculture sector requires the use of novel and sustainable ingredients in aquaculture feeds. A study was undertaken to evaluate the effect of a 10% incorporation of defatted microalgal biomass (DMB) of Tetraselmis sp. CTP4, used at the expense of dehulled solvent-extracted soybean meal (SBM), on the growth performance...
Article
Full-text available
Microalgae are microorganisms with the capacity to contribute to the sustainable and healthy food production, in addition to wastewater treatment. The subject of this work was to determine the potential of Scenedesmus obliquus microalga grown in brewery wastewater to act as a plant biostimulant. The germination index of watercress seeds, as well as...
Article
Full-text available
Six microalgae strains were screened according to their biomass productivity and polymer synthesis, showing biomass productivity between 0.14 and 0.68 g/(L·d) for a 21-day growth period. Extracellular biopolymers from the spent culture media of Nostoc sp. (No), Synechocystis sp. (Sy), and Porphyridium purpureum (Pp) was obtained, and the yields of...
Chapter
Conventional wastewater treatment (WWT) is currently based on the action of heterotrophic and nitrifying bacteria supported by mechanical aeration in a sequence of interconnected aerobic, anoxic, and anaerobic processes. It provided satisfactory levels of pollutant removal, but at the expenses of high-energy consumption and environmental impacts (h...
Chapter
Microalgae-based bioproducts remain expensive mainly due to microalgae cultivation, harvesting, and downstream processing costs. Nonetheless, microalgae are a high potential source of several biofuels, biofertilizers, and bioproducts (e.g., carbohydrates, long-chain fatty acids, pigments, and proteins), which can provide important nutritional, cosm...
Article
In the present study, the sustainability of the simultaneously upgrading of biogas and high-value algal biomass production was evaluated in terms of energy consumption, CO2 emissions and environmental impacts. Two pilot-scale biogas upgrading units and a simulated real scale plant were considered. The study included the creation of an inventory of...
Conference Paper
Microalga Scenedesmus obliquus cultivated in brewery effluents. The microalga was tested as a biofertilizer/biostimulant and showed an enhancing effect on seed germination and plant growth (number, length and leaves).
Article
Full-text available
Commercial production of microalgal biomass for food and feed is a recent worldwide trend. Although it is common to publish nutritional data for microalgae grown at the lab-scale, data about industrial strains cultivated in an industrial setting are scarce in the literature. Thus, here we present the nutritional composition and a microbiological an...
Article
The circular bioeconomy concept relies on the exploitation of wastes as a feedstock of different biotechnological processes to obtain, as much as possible, a huge spectrum of biochemical components through a biorefinery platform. This work deals with the treatment of brewery effluent through the cultivation of Scenedesmus obliquus microalga and the...
Conference Paper
It has been shown that microalga S. obliquus presented bioactive compounds which had an enhancing effect on seed germination and plant growth (number, length and leaves). Moreover, there was an increase of 40% on germination of watercress (Lepidium sativum) using the biomass at 0.1 g/L, without any pre-treatment steps.
Article
Full-text available
Biomass harvesting is one of the most expensive steps of the whole microalgal production pipeline. Therefore, the present work aimed to understand the effect of salinity on the growth performance, biochemical composition and sedimentation velocity of Tetraselmis sp. CTP4, in order to establish an effective low-cost pilot-scale harvesting system for...
Chapter
Microalgae accumulate a variety of compounds that form the basis for potential marketable products as whole cell biomass or as fractions after an extraction process. A cascading biorefinery approach aims at recovering and separating multiple algae compounds to valorise the complete biomass and to improve the economics of the value chain. This chapt...
Article
Wastewater biological treatment with microalgae can be an effective technology, removing nutrients and other contaminants while reducing chemical oxygen demand. This can be particularly interesting for the meat producing industry which produces large volumes of wastewater from the slaughtering of animals and cleaning of their facilities. The main p...
Article
Full-text available
Microalgae have been used to remove nitrogen, phosphorus, and chemical oxygen demand (COD) from brewery wastewater (BWW). The microalga Scenedesmus obliquus was grown on BWW, using bubble column photobioreactors that operated under batch and continuous regimes. For the first time, the cell physiological status cell membrane integrity and enzymatic...
Article
Full-text available
Industrial production of novel microalgal isolates is key to improving the current portfolio of available strains that are able to grow in large-scale production systems for different biotechnological applications, including carbon mitigation. In this context, Tetraselmis sp. CTP4 was successfully scaled up from an agar plate to 35- and 100-m3indus...
Presentation
Tetraselmis sp. CTP4 is a euryhaline microalgal strain recently isolated from a coastal lagoon (Ria Formosa, Portugal) by means of fluorescence activated cell sorting with high potential for different biotechnological applications. Laboratory experiments revealed that this strain displays a high growth performance and robustness, namely a wide tole...
Article
In this work, hydrogen (H2) was produced through the fermentation of Spirogyra sp. biomass by Clostridium butyricum DSM 10702. Macronutrient stress was applied to increase the carbohydrate content in Spirogyra, and a 36% (w/w) accumulation of carbohydrates was reached by nitrogen depletion. The use of wet microalga as fermentable substrate was comp...
Article
The ability of microalgae to grow in nutrient-rich environments and to accumulate nutrients from wastewaters (WW) makes them attractive for the sustainable and low-cost treatment of WW. The valuable biomass produced can be further used for the generation of bioenergy, animal feed, fertilizers, and biopolymers, among others. In this study, Scenedesm...
Chapter
Microalgae harvesting is a major challenge because microalgal cells are small and carry a negative surface charge and biomass concentration in cultures is relatively low. The microalgal biomass (0.05%, w/w) needs to be concentrated to a paste with 15%–25% water content. This dewatering process is ideally performed in two stages, including a first p...
Chapter
Full-text available
Microalgae have been proposed as a multiproduct biorefinery feed stock. The algae-based biorefinery concept relies on the complete optimization process from the biomass production to processing and generating different products. Microalgae are composed of a high number of biocompounds with numerous applications. It has been pointed out by several r...
Chapter
Research on biological processes for wastewater treatment (WWT) over the past decade has focused on the development of compact treatment processes at the expense of a high energy demand or a loss of valuable nutrients. The recent worldwide interest in the cultivation of microalgae for energy production, along with the need for more sustainable WWT...
Chapter
The economic analysis of biomass production is a critical step in ensuring the success of any microalgae-based industry. Until recently, only small-scale facilities of less than 10 ha have been in operation, but now large-scale facilities of more than 200 ha are being built and operated. Moreover, specific machinery and technologies are being adapt...
Chapter
This chapter focuses on the production of microalgae-based biodiesel, which has been the most studied biofuel from this promising feedstock. This chapter highlights the advantages of the microalgal biomass to produce third-generation biodiesel compared to oleaginous crops (first generation) and to other raw materials (second generation). The differ...
Presentation
Bioprospection of novel microalgal isolates and industrial production in large-scale production systems is key to improving the current portfolio of available strains for different biotechnological applications. In this context, a novel Tetraselmis sp. CTP4 isolate was successfully scaled up from an agar plate to 2.5-, 35-, 100- and 150-m3 tubular...
Article
Biohydrogen production through dark fermentation is a promising technology for generating renewable energy, while using microalgal biomass as a third generation feedstock can further increase the sustainability of the process. In the present study, Scenedesmus obliquus was used as model microalga substrate for studying the impact of operational par...
Article
The atmospheric CO2 increase is considered the main cause of global warming effect. Microalgae are photosynthetic microorganisms that can help to CO2 mitigation and at the same time produce value-added compounds. In this study, Scenedesmus obliquus, Chlorella vulgaris and Chlorella protothecoides were cultivated under 0.035 (air), 5% and 10% (v/v)...
Article
Full-text available
Diacronema vlkianum is a marine microalgae for which supposed health promoting effects have been claimed based on its phytochemical composition. The potential use of its biomass as health ingredient, including detox-shakes, and the lack of bioavailability studies were the main concerns. In order to evaluate the microalgae-biomass assimilation and i...
Article
Microalgae can be used for wastewater bioremediation with simultaneous CO2 biofixation producing valuable biomass. Wastewater from a brewery was treated using the Scenedesmus obliquus microalga in bubble-column photobioreactors (PBRs). The PBRs were fed with ambient air and the effect of a 10% (v/v) brewery CO2 supplement was studied. The PBRs were...
Chapter
Microalgae have been proposed as a multi-product biorefinery feed-stock. The algae-based biorefinery concept relies on the complete process optimization from the biomass production to the processing and generation of different products. Microalgae are composed by a high number of biocompounds, those with numerous applications. It has been pointed o...
Chapter
The biorefinery concept harbors a wide range of conversion technologies in order to take advantage of all the components of a feedstock . Among those technologies, a wide range of biological/biochemical processes can be envisaged to generate products such as biofuels, value-added products , and other chemical building blocks . These processes are g...
Article
Full-text available
Bioprospecting for novel microalgal strains is key to improving the feasibility of microalgae-derived biodiesel production. Tetraselmis sp. CTP4 (Chlorophyta, Chlorodendrophyceae) was isolated using fluorescence activated cell sorting (FACS) in order to screen novel lipid-rich microalgae. CTP4 is a robust, euryhaline strain able to grow in seawater...
Article
This work is part of a LIFE project to treat urban wastewater from Águas da Figueira (AdF, Figueira da Foz, PT) using a vertical tubular photobioreactor (PBR) prototype (150 L), to be scaled up and integrated in a waste water treatment plant (WWTP). The PBR was inoculated with three different microalgae: Chlorella vulgaris (Cv), Scenedesmus obliquu...
Article
The potential of Nannochloropsis sp. microalga to biodiesel production was assessed. The work deals with the optimization of the cell disruption, a required step when the lipid extraction followed by the transesterification reaction is the option to produce biodiesel, as well as the optimization of the reaction parameters (catalyst, alcohol, temper...
Poster
Bioprospecting for novel microalgae strains is of the outmost importance. Via a high throughput methodology, 96 strains of marine microalgae have been successfully isolated from local coastal waters. Among all isolates, strain CTP4 classified as Tetraselmis sp. by 18S rDNA sequencing showed to be an ideal strain for biotechnological applications, d...
Conference Paper
Full-text available
The aim of this work was to study the bioremediation of effluents from the poultry processing industry using two microalgae (Chlorella vulgaris and Chlorella protothecoides). In addition, the microalgal biomass obtained will be evaluated for the production of feed, fertilizer and/or biofuels. Microalgae were grown at lab scale in order to select th...
Article
The biological hydrogen production from Spirogyra sp. biomass was studied in a SBR (sequential batch reactor) equipped with a biogas collecting and storage system. Two acid hydrolysis pre-treatments (1N and 2N H2SO4) were applied to the Spirogyra biomass and the subsequent fermentation by Clostridium butyricum DSM 10702 was compared. The 1N and 2N...
Conference Paper
Full-text available
As microalgas ricas em lípidos podem ser diretamente utilizadas para produzir biodiesel, no entanto a sua utilização noutras aplicações, no setor farmacêutico e alimentar, podem ser também particularmente interessantes. Este trabalho tem como objetivo contribuir para a sustentabilidade do tratamento de efluentes residuais da indústria do processame...
Article
Full-text available
Autotrophic microalgae are photosynthetic organisms that undergo the conversion of light into chemical energy as a form of a wide range of organic compounds through its photosynthetic machinery. The cultivation of microalgae brings environmental advantages, bearing in mind the capability of nutrient recycling in wastewaters together with the fixati...
Article
Scenedesmus obliquus biomass was used as a feedstock for comparing the biological production of hydrogen by two different types of anaerobic cultures: a heat-treated mixed culture from a wastewater treatment plant and Clostridium butyricum DSM 10702. The influence of the incubation temperature and the carbon source composition were evaluated in ord...
Article
The aim of the present work was the simultaneous treatment of urban wastewater using microalgae and the energetic valorization of the obtained biomass. Chlorella vulgaris (Cv), Scenedesmus obliquus (Sc) and a naturally occurring algal Consortium C (ConsC) were grown in an urban wastewater. The nutrient removals were quite high and the treated water...
Article
Hydrogen (H2) gas is seen as an ideal future energy carrier because it is easily converted into electricity in fuel cells, liberates a large amount of energy per unit mass, and generates no air pollutants. In this work, biological hydrogen (bioH2) was produced from the microalgal biomass of Scenedesmus obliquus which was used as a substrate for the...
Article
Full-text available
The production of microbial biofuels is currently under investigation, as they are alternative sources to fossil fuels, which are diminishing and their use has a negative impact on the environment. However, so far, biofuels derived from microbes are not economically competitive. One way to overcome this bottleneck is the use of microorganisms to tr...
Article
Full-text available
Isoprene is a key intermediate compound for the production of synthetic rubber and adhesives and is also used as a building block in the chemical industry. Traditionally, isoprene is obtained from crude oil during the refinery process. Nevertheless, plants and animals are also able to synthesize this important compound. This work compares two renew...
Article
Chlorella vulgaris (Cv) biomass is widely used as a traditional food in the Orient and its safety for human consumption has been proved. However, carotenogenic biomass (orange), resulting from induced stresses, needs further safety evaluation, which is the aim of this study. The preliminary toxicological characterization of Cv consisted of a single...
Article
This paper presents a life cycle inventory of biohydrogen production by Clostridium butyricum through the fermentation of the whole Scenedesmus obliquus biomass. The main purpose of this work was to determine the energy consumption and CO2 emissions during the production of hydrogen. This was accomplished through the fermentation of the microalgal...
Article
Microalgae are one of the most promising sources for new food and functional food products, and can be used to enhance the nutritional value of foods, due to their well-balanced chemical composition. Knowing their physicochemical characteristics is fundamental for the selection of the most suitable microalgae to specific food technology application...
Data
a b s t r a c t Factors such as an ageing population, increased health care costs and rapid advances in science and technology are likely driving the increase interest among consumers in attaining wellness through diet, which is in turn, fuelling interest in functional foods and changing the way that people eat. Microalgae have been largely culture...
Article
This paper presents the life cycle inventory (LCI) of hydrogen production by Clostridium butyricum fermentation of Scenedesmus obliquus hydrolysate. The main purpose of this work was to evaluate the potential of H2 production from microalgal biomass and the respective energy consumption and CO2 emissions in the bioconversion process considering the...
Article
Full-text available
Microalgae (prokaryotic and eukaryotic) consist of a wide range of autotrophic organisms which grow through photosynthesis just like land based plants. Their unicellular structure allows them to easily convert solar energy into chemical energy through CO2 fixation and O2 evolution, being well adapted to capture CO2 and store it as biomass. Microalg...