Luis H Reyes

Luis H Reyes
Los Andes University (Colombia) | UNIANDES · Department of Chemical and Food Engineering

Doctor of Philosophy

About

70
Publications
10,491
Reads
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893
Citations
Additional affiliations
July 2017 - June 2020
Los Andes University (Colombia)
Position
  • Professor (Assistant)
July 2016 - June 2017
Universidad de América
Position
  • Lecturer
April 2015 - April 2017
Pontificia Universidad Javeriana
Position
  • PostDoc Position
Education
August 2008 - August 2013
Texas A&M University
Field of study
  • Chemical Engineering
March 2001 - September 2007
Industrial University of Santander
Field of study
  • Chemical Engineering

Publications

Publications (70)
Article
Full-text available
At the beginning of 2020, the pandemic caused by the SARS-CoV-2 virus led to the fast sequencing of its genome to facilitate molecular engineering strategies to control the pathogen’s spread. The spike (S) glycoprotein has been identified as the leading therapeutic agent due to its role in localizing the ACE2 receptor in the host’s pulmonary cell m...
Preprint
Antimicrobial peptides (AMPs) have emerged as exciting alternatives to the alarming increase of multiresistant bacteria due to their high activity against them through mechanisms that are thought to largely avoid resistance in the long term. Buforin II (BUFII) is an antibacterial peptide hypothesized to kill bacteria by crossing their membranes to...
Article
Full-text available
Antibiotic resistance has become a major public health problem generated by their excessive and inappropriate use. This is worrisome because multiple microbial infections that could traditionally be treated without major complications are now considerably challenging to treat. In this regard, research in this field has been focused on searching for...
Article
Full-text available
Biomaterials engineering and biotechnology have advanced significantly towards probiotic encapsulation with encouraging results in assuring sufficient bioactivity. However, some major challenges remain to be addressed, and these include maintaining stability in different compartments of the gastrointestinal tract (GIT), favoring adhesion only at th...
Article
Full-text available
Background Mucopolysaccharidosis type II (MPS II), also known as Hunter syndrome, is a rare X-linked recessive disease caused by a deficiency of the lysosomal enzyme iduronate-2-sulfatase (IDS), which activates intracellular accumulation of nonmetabolized glycosaminoglycans such as heparan sulfate and dermatan sulfate. This accumulation causes seve...
Article
Full-text available
Dairy products are among the most popular nutritious foods in the world. Understanding the relationship between the composition, process, and structural properties at different scales (molecular, microscopic, and macroscopic) is fundamental to designing dairy products. This review highlights the need to analyze this relationship from different scal...
Article
Full-text available
Fructo-oligosaccharides (FOS) are one of the most well-studied and commercialized prebiotics. FOS can be obtained either by controlled hydrolysis of inulin or by sucrose transfructosylation. FOS produced from sucrose are typically classified as short-chain FOS (scFOS), of which the best known are 1-kestotriose (GF2), 1,1-kestotetraose (GF3), and 1,...
Article
Full-text available
Magnetite nanoparticles (MNPs) have gained significant attention in several applications for drug delivery. However, there are some issues related to cell penetration, especially in the transport of cargoes that show limited membrane passing. A widely studied strategy to overcome this problem is the encapsulation of the MNPs into liposomes to form...
Article
Full-text available
As life expectancy continues to increase, the inevitable weakening and rupture of bone tissue have grown as concerns in the medical community, thus leading to the need for adhesive materials suitable for bone repair applications. However, current commercially available adhesives face certain drawbacks that prevent proper tissue repair, such as low...
Article
Full-text available
Gelatin and chitosan nanoparticles have been widely used in pharmaceutical, biomedical, and nanofood applications due to their high biocompatibility and biodegradability. This study proposed a highly efficient synthesis method for type B gelatin and low-molecular-weight (LMW) chitosan nanoparticles. Gelatin nanoparticles (GNPs) were synthesized by...
Article
Full-text available
The discovery of new membrane-active peptides (MAPs) is an area of considerable interest in modern biotechnology considering their ample applicability in several fields ranging from the development of novel delivery vehicles (via cell-penetrating peptides) to responding to the latent threat of antibiotic resistance (via antimicrobial peptides). Dif...
Article
Full-text available
Important institutions, such as the World Health Organization, recommend reducing alcohol consumption by encouraging healthier drinking habits. This could be achieved, for example, by employing more effective promotion of non-alcoholic beverages. For such purposes, in this study, we assessed the role of experiential beer packaging sounds during the...
Article
Full-text available
One of the main routes to ensure that biomolecules or bioactive agents remain active as they are incorporated into products with applications in different industries is by their encapsulation. Liposomes are attractive platforms for encapsulation due to their ease of synthesis and manipulation and the potential to fuse with cell membranes when they...
Article
Full-text available
CRISPR is a simple and cost-efficient gene-editing technique that has become increasingly popular over the last decades. Various CRISPR/Cas-based applications have been developed to introduce changes in the genome and alter gene expression in diverse systems and tissues. These novel gene-editing techniques are particularly promising for investigati...
Article
Full-text available
Nutraceutical formulations based on probiotic microorganisms have gained significant attention over the past decade due to their beneficial properties on human health. Yeasts offer some advantages over other probiotic organisms, such as immunomodulatory properties, anticancer effects and effective suppression of pathogens. However, one of the main...
Article
Full-text available
Gene therapy has been used as a potential approach to address the diagnosis and treatment of genetic diseases and inherited disorders. In this line, non-viral systems have been exploited as promising alternatives for delivering therapeutic transgenes and proteins. In this review, we explored how biological barriers are effectively overcome by non-v...
Article
Full-text available
The development of a culture medium is an essential step in any bioprocess involving microorganisms for the bioconversion of by‐products to valuable chemicals, making industries like the biofuel industry more competitive. Optimization of the bioconversion process to minimize cost while maximizing yield underscores the importance of using computatio...
Article
Full-text available
Mucopolysaccharidosis type II (MPS II), also known as Hunter syndrome, is a rare X-linked recessive disease caused by a deficiency of the lysosomal enzyme iduronate-2-sulfatase (IDS). This deficiency leads to a progressive intracellular accumulation of the glycosaminoglycans heparan sulfate and dermatan sulfate, causing severe damage to the central...
Article
Full-text available
The effects on the texture, rheology, and microstructure of costeño-type artisan cheese caused by the substitution and reduction of NaCl and the increase in cooking temperature during cheese production were studied using a multiscale approach that correlates responses at the macroscopic and microscopic levels. The decrease in the NaCl content, the...
Article
Full-text available
The development of a comprehensive set of skills, including technical, professional, and technology expertise, is critical to succeeding in the increasingly competitive global job marketplace. We proposed to develop such skills in our junior students (third year) via a flipped-classroom approach, a PO-PBL problem, and interactive e-learning tools....
Article
Full-text available
One of the challenges of modern biotechnology is to find new routes to mitigate the resistance to conventional antibiotics. Antimicrobial peptides (AMPs) are an alternative type of biomolecules, naturally present in a wide variety of organisms, with the capacity to overcome the current microorganism resistance threat. Here, we reviewed our recent e...
Conference Paper
Full-text available
Gene therapy has been considered a promising strategy for treating several inherited diseases and acquired complex disorders. One crucial challenge yet to be solved to ensure the nanomaterials’ success in delivering gene therapies is their ability to escape from endosomes. To address this issue, we previously developed magnetite nanoparticles conju...
Conference Paper
Full-text available
One of the main challenges in gene therapy is the transport of genetic material into target cells. This is mainly due to the need for overcoming several obstacles like rapid genetic material degradation by the physiological environment, low endosomal escape, and limited cell uptake. A meaningful way to increase the efficacy of genetic material deli...
Conference Paper
Full-text available
Nowadays, nanoparticles (NPs) are used to make safe and more effective biomedical technologies for applications in highly targeted therapeutics and drug-delivery vehicles. This helps avoid low cellular penetration and accumulation of the drug in intracellular endosomal compartments that are not of interest to a particular therapy. A way to enhance...
Conference Paper
Full-text available
The delivery of bioactive compounds is often improved by their encapsulation within systems based on different materials, such as polymers and phospholipids. In this regard, one of the most attractive vehicles are liposomes, which can be produced by the self-assembly of phospholipids in aqueous buffered systems. Encapsulation of therapeutic magneti...
Conference Paper
Full-text available
Several biological barriers are generally responsible for the limited delivery of cargoes at the cellular level. Fullerenols have unique structural features and possess suitable properties for interaction with the cells. This study aimed to synthesize and characterize a fullerenol derivative with desirable characteristics (size, charge, functionali...
Conference Paper
Full-text available
Magnetite nanoparticles (MNPs) have been considered for several applications in drug delivery. However, the main challenge is to assure high cell-penetration levels, especially when dealing with cargoes that show limited membrane passing. A strategy is to encapsulate the MNPs into liposomes to form magnetoliposomes (MLs) capable of fusing with memb...
Conference Paper
Full-text available
The encapsulation of biomolecules and microorganisms into liposomes is useful for several biological and biomedical applications. For instance, it is possible to encapsulate pharmacological compounds to increase properties such as therapeutic effectiveness, circulation times, and biocompatibility. Here, we are interested in encapsulating yeast cell...
Conference Paper
Full-text available
Magnetite nanoparticles (MNPs) have been considered for several applications in drug delivery. However, the main challenge is to assure high cell-penetration levels, especially when dealing with cargoes that show limited membrane passing. A strategy is to encapsulate the MNPs into liposomes to form magnetoliposomes (MLs) capable of fusing with memb...
Article
Full-text available
Los problemas asociados a la producción masiva de porquinaza en las granjas porcícolas se asocian al mal olor, a la propagación de patógenos y a la contaminación de fuentes hídricas y suelos. Como una solución sostenible se implementó un biodigestor con el fin de aprovechar el biosol producido por biodigestión anaerobia de porquinaza y promover la...
Article
Many researchers have limited access to fully equipped laboratory-scale batch bioreactors and chemostats due to their relatively high cost. This becomes particularly prohibitive when multiple replicas of the same experiment are required, but not enough bioreactors are available to operate simultaneously. Additionally, experiments using shaken flask...
Article
Full-text available
Preparing work-ready chemical engineering graduates is achieved by integrating the technical skills and knowledge learned at university with employability skills required by industry. While this is most often made through industry-placements, non-placement forms of work-integrated learning (WIL) can be highly effective in preparing graduates for th...
Article
Full-text available
Nanomedicine is entering a high maturity stage and is ready to reach full translation into the clinical practice. This is because of the ample spectrum of applications enabled by a large arsenal of nanostructured materials. In particular, bimetallic patchy core/shell nanoparticles offer tunable surfaces that allow multifunctional responses. Despite...
Article
Full-text available
Clostridium (Ruminiclostridium) thermocellum is recognized for its ability to ferment cellulosic biomass directly, but it cannot naturally grow on xylose. Recently, C. thermocellum (KJC335) was engineered to utilize xylose through expressing a heterologous xylose catabolizing pathway. Here, we compared KJC335′s transcriptomic responses to xylose ve...
Article
Full-text available
Mixing operations in biological processes is of utmost importance due to its effect on scaling-up and heat and mass transfer. This paper presents the characterization of a bench-top bioreactor with different impeller configurations, agitation and oxygen transfer rates, using CFD simulations and experimental procedures. Here, it is demonstrated that...
Article
Full-text available
Lysosomal storage disorders (LSDs) are a group of monogenic diseases characterized by progressive accumulation of undegraded substrates into the lysosome, due to mutations in genes that encode for proteins involved in normal lysosomal function. In recent years, several approaches have been explored to find effective and successful therapies, includ...
Article
Full-text available
Over the past decade, gene therapies have attracted much attention for the development of treatments for various conditions, including cancer, neurodegenerative diseases, protein deficiencies, and autoimmune disorders. Despite the benefits of this approach, several challenges are yet to be solved to reach clinical implementation. Some of these chal...
Article
Full-text available
One of the main issues when orally administering microorganism-based probiotics is the significant loss of bioactivity as they pass through the gastrointestinal (GI) tract. To overcome these issues, here, we propose to encapsulate the probiotic yeast Kluyveromyces lactis on chemically crosslinked gelatin hydrogels as a means to protect the bioactiv...
Article
Full-text available
Chemical products such as PEO/PPO block copolymers or alkoxylated resins tend to work well in chemical demulsification of light hydrocarbons (°API > 20) but fail to do so in the presence of heavy and extra heavy crude emulsions. Moreover, a large knowledge gap between the relationship of instability mechanisms at the liquid-liquid interface and the...
Conference Paper
Full-text available
Currently, there is a growing interest in science and industry to generate new products and processes that can be used as efficient and green sources of energy. This is also important to find avenues to substitute fossil fuel consumption as their reserves are rapidly declining and the environmental impact issues associated with their intensive use...
Conference Paper
Full-text available
Over the past few years, gene therapies have attracted much attention for the development of therapies for various conditions including cancer, neurodegenerative diseases, protein deficiencies and autoimmune disorders. Despite the benefits of this approach, several challenges are yet to be solved to eventually reach clinical implementation. Some of...
Conference Paper
Full-text available
The need for platforms to scale-up bioprocesses is of the utmost importance for proof-of-concept experiments to collect information regarding operating conditions in order to maximize yield and minimize energy consumption. Commercially available systems are expensive, require large volumes and rely on tedious assembly protocols. This is particularl...
Conference Paper
Full-text available
Unit Operations and Modelling and Simulation have been for long time a staple of the academic formation of any chemical engineer. Both are integral to the analysis of any chemical process, discretizing it into smaller specific processes that can then be characterized and modelled through the solution of balance, thermodynamics and transport equatio...
Conference Paper
Full-text available
Pig farming is one of the most productive sectors of the Colombian economy, which faces quality and health challenges, due to the environmental impact generated by the inadequate management of the waste produced by the swine manure that contaminates the bodies of water, soil and air. This is the productive activity of the habitants of the Verjón Fo...
Article
Full-text available
Iduronate-2-sulfatase (IDS) is a lysosomal enzyme involved in the metabolism of the glycosaminoglycans heparan (HS) and dermatan (DS) sulfate. Mutations on IDS gene produce mucopolysaccharidosis II (MPS II), characterized by the lysosomal accumulation of HS and DS, leading to severe damage of the central nervous system (CNS) and other tissues. In t...
Article
Full-text available
Unit Operations and Modelling and Simulation have been for long time a staple of the academic formation of any chemical engineer. Both are integral to the analysis of any chemical process, discretizing it into smaller specific processes that can then be characterized and modelled through the solution of balance, thermodynamics and transport equatio...
Conference Paper
Full-text available
Cellulose and hemicellulose are the most abundant components in plant biomass. A preferred Consolidated Bioprocessing (CBP) system is one which can directly convert both cellulose and hemicellulose into target products without adding the costly hydrolytic enzyme cocktail. In this work, the thermophilic, cellulolytic, and anaerobic bacterium, Clostr...
Article
Full-text available
Cellulose and hemicellulose are the most abundant components in plant biomass. A preferred Consolidated Bioprocessing (CBP) system is one which can directly convert both cellulose and hemicellulose into target products without adding the costly hydrolytic enzyme cocktail. In this work, the thermophilic, cellulolytic, and anaerobic bacterium, Clostr...
Chapter
Full-text available
Adaptive laboratory evolution is a powerful technique for strain development. However, the target phenotypes using this strategy have been limited by the required coupling of the phenotype-of-interest with fitness or survival, and thus adaptive evolution is generally not used to improve product formation. If the desired product confers a benefit to...
Article
Full-text available
Previously, we demonstrated production of an active recombinant human N-acetylgalactosamine-6-sulfatase (rhGALNS) enzyme in Escherichia coli as a potential therapeutic alternative for mucopolysaccharidosis IVA. However, most of the rhGALNS produced was present as protein aggregates. Here, several methods were investigated to improve production and...
Article
Full-text available
An evolutionary engineering approach for enhancing heterologous carotenoids production in an engineered Saccharomyces cerevisiae strain was used previously to isolate several carotenoids hyper-producers from the evolved populations. β-Carotene production was characterized in the parental and one of the evolved carotenoids hyper-producers (SM14) usi...