Luis guillermo gonzález-olivares

• Doctor of Biotechnology
• Researcher at Autonomous University of Hidalgo State

Nowadays, probiotics have been utilized as starter cultures in the elaboration of fermented dairy products such as cheese. The survival of probiotic microorganisms in this type of products is very important in order to have a beneficial effect after their consumption. In addition to this, milk proteins are considered an important source of bioactiv...

The aim of this study was to evaluate the release of bioactive peptides encrypted in milk proteins by proteolytic activity of lactic acid bacteria in commercial fermented milks in refrigerated storage. Peptide concentration varied from 0.05 mg / ml in LF–3 (Lactobacillus casei) and LF–4 (Lactobacillus acidophilus) to 0.227 mg/ml in LF–5 (Lactobacil...

Milk is an important source of bioactive compounds. Many of these compounds are released during fermentation and refrigerated storage. The aim of this study was to determine the release of peptides by lactic acid bacteria in commercial fermented milk during refrigerated storage. The size and profile of peptides were analyzed by polyacrylamide gel e...

Selenium is an essential micronutrient which is found in many foods and beverages in low concentrations. Craft beer, one of the most widely consumed fermented beverages globally, presents a strategic opportunity for selenium intake through organic nanoparticles. This study aimed to confirm the presence of selenium nanoparticles in the fermentation...

The increasing interest in the health benefits of whey-derived bioactive peptides highlighted the need for a comprehensive understanding of their production and applications. This study analyzed the global research landscape on the enzymatic hydrolysis of bovine whey and its bioactive peptides from 2004 to 2024. A bibliometric approach was used to...

The growing consumer demand for natural food preservatives has intensified research into bacteriocins, due to their potential to enhance food safety and preservation. This study aimed to conduct a bibliometric analysis of bacteriocin research from 2003 to 2023, focusing on their applications in food preservation to identify critical trends, challen...

The increasing demand for functional foods has led to an interest in fermentation processes that enhance the production of antioxidant peptides. This study aimed to perform a bibliometric analysis of lactic acid bacteria (LAB) used in the fermentation of wheat to produce antioxidant peptides. To achieve this, data from 2010 to 2023 were gathered fr...

La presente obra contribuye a fortalecer la Línea de Generación y Aplicación del Conocimiento (LGAC) denominada Caracterización de Productos Alimenticios y Técnicas Culinarias que forma parte del Grupo de Investigación del Programa Educativo de Gastronomía de la Universidad Autónoma del Estado de Hidalgo, del Instituto de Ciencias Económico Adminis...

Gummies are a popular product in the confectionery industry, characterized by their various flavors and attractive shapes. The main challenge in their production is balancing ingredients and optimizing processes to ensure excellent texture, taste, aroma, and quality while adapting to the demand for healthier and more sustainable products. This cont...

Flowers have been commonly used in cooking to add color and flavor to dishes. In addition to enhancing the visual appeal of food, many edible flowers also contain bioactive compounds that promote good health. These compounds include antimicrobial, antihypertensive, nephroprotective, antiulcer, and anticancer agents. In the last 5 years, there have...

El mango Ataúlfo es altamente valorado por su color amarillo intenso, el cual es un indicador clave de su madurez y calidad. El objetivo de este estudio fue modelar los cambios de color en el mango Ataúlfo durante el almacenamiento, utilizando modelos cinéticos y técnicas de procesamiento de imágenes con OpenCV en Python, para cuantificar y describ...

Fermenting fruit juices with lactic acid bacteria (LAB) is a sustainable method to enhance fruit harvests and extend shelf life. This study focused on blackberries, rich in antioxidants with proven health benefits. In this research, we examined the effects of fermentation (48 h at 37 °C) at 28 days on whey-supplemented (WH, 1:1) blackberry juice (B...

Given the rising interest in functional foods for health benefits, this study aims to evaluate the antihypertensive activity of an ice cream base incorporating Lacticaseibacillus rhamnosus GG and aguamiel syrup. We assessed the probiotic viability and ACE inhibitory activity in ice cream enriched with aguamiel syrup compared to inulin. Several repo...

El mangostán (Garcinia mangostana) es un fruto que se considera exótico en México, sin embargo, empieza a tener relevancia por las propiedades que aporta. Debido a la importancia que está cobrando este fruto, en el presente trabajo se realizó el análisis proximal de su pulpa y de su cáscara, así como la medida de la capacidad antioxidante (FRAP y D...

This chapter addressed the problem of understanding the role of probiotics in managing metabolic syndrome. Therefore, the objective was to analyze the clinical evidence surrounding using probiotics and prebiotics for metabolic syndrome through a bibliometric analysis and to evaluate the impact of dietary interventions on the microbiota. The most si...

Background Standard cancer treatments show a lack of selectivity that has led to the search for new strategies against cancer. The selective elimination of cancer cells modulating the redox environment, known as “selective oxycution”, has emerged as a viable alternative. This research focuses on characterizing the unexplored Escallonia genus plant...

The objective of this study was to determine the optimal conditions of the extrusion cooking process using ungerminated Lupinus angustifolius flour, to later develop snacks with germinated flours for 3, 5 and 7 days. The optimal conditions were 16% feed moisture, 152 ◦C die temperature, and 47/53% lupinus/corn starch proportion. The water absorptio...

Foods based on plant proteins have increased in recent years. Thus, this study aimed to establish the potential of rye protein hydrolysates to release bioactive peptides to treat non-communicable diseases. Rye protein isolate was obtained using the solubilization–precipitation method and hydrolyzed with alcalase and flavourzyme. Hydrolysates were a...

Bovine whey remains a common pollutant from food, and diverse strategies to mitigate it have been formed. Therefore, this study aimed to generate a functional ingredient from whey hydrolysis to be incorporated into beverage formulations. Hydrolysis at pH = 8 for 7 h was performed in order to determine the sample to be added to the beverage. It was...

Selenium is an essential trace element in human health. However, it has been considered a widespread selenium deficiency worldwide, although the recommended daily intake is very low (55 μg per day). Strategies have been implemented to comply with the recommended doses, for example, through bioavailable selenium such as selenoamino acids. Thus, this...

This work analyzed the antidiabetic activity of peptides from whey proteins after hydrolysis by Lactobacillus rhamnosus GG and Streptococcus thermophilus SY-102, emphasizing the differences between the proteolytic systems of both bacteria. Peptide fractions from whey proteins may have biological functions, such as antidiabetic functions, which inhi...

Different carbon sources, such as prebiotics, have promoted probiotics’ survival during refrigerated fermented milk storage. These compounds stimulate both the metabolic response and the resistance of probiotics to adverse conditions, such as low temperatures. That is why the objective of this study was to evaluate the kinetic parameters of growth,...

The aim of this work was to find out if biofilms can be made by lactic acid bacteria (LAB) isolated from agave plants using agave fructans as sole carbohydrate substrates or if it was necessary to use fructose as a breakdown product of such polymers. This is part of a research project geared to develop industrial lactic acid production from agave f...

Lactic acid bacteria (LAB) resist sodium selenite of concentrations greater than 100 mg/L in fermentation media. Selenium affects the growth rate, but once the microorganism absorbs selenium, this element is converted through a complex mechanism into selenocysteine and then into a selenoprotein structure. This study verified the presence of selenoc...

Un Muffin es un pastel pequeño redondo sin azúcar. En general, los productos panificados han sido objeto de diversas investigaciones debido a la interacción entre sus componentes y su deterioro. Puesto que en la presente investigación fueron estudiados unos muffins de vainilla con una buena estabilidad microbiológica gracias a los agentes conservan...

Many studies have reported the benefits of probiotic microorganisms and the production of angiotensin-converting enzyme (ACE) inhibitors. Determining the proteolytic and ACE inhibition capacities during whey fermentation was the goal of the study. Lacticaseibacillus rhamnosus GG, Streptococcus thermophilus SY-102, and both bacteria together were in...

Los ácidos húmicos (AHs) son los componentes principales y más abundantes de la materia orgánica (MO). En este estudio se realizó una evaluación de AHs mediante parámetros espectroscópicos, posteriormente a un proceso de aglomeración, coagulación y microfiltración en presencia de hierro (Fe3+) como agente coagulante y mediante la cuantificación por...

In recent years, the synthesis of human milk oligosaccharides, particularly fucooligosaccharides, has been investigated. These oligosaccharides provide protection against gastrointestinal diseases for newborns, among other functionalities. α-L-fucosidase from Thermotoga maritima of GH29 family has been reported to produce fucooligosaccharides by me...

In this work, the ability to biodegrade two samples of polystyrene (PS) was evaluated: crystal and expanded with three bacterial strains isolated from PS waste collected in the municipal dump of Chimalhuacán, Estado de Mexico. The biodegradation potential of the isolated strains was determined by substrate weight loss assays. All bacterial strains...

Human milk is the ideal food for newborns. In addition, thanks to the fucooligosaccharides it contains, it provides protection against gastrointestinal diseases. These oligosaccharides can be synthesized enzymatically using α-L-fucosidases. The aim of this work was to compare the active site of three fucosidases of the GH29 family with in silico me...

Selenium is essential for human health; however, recommended daily intake is not always met. Thus, studies have beencarried out on the biogenic production of more bioavailable selenium. It has been demonstrated that certain lactobacilli canmetabolize inorganic selenium to transform it into selenoamino acids. This study aimed to add selenium onLacto...

Abstract Lactic acid bacteria have been studied for their ability to accumulate inorganic selenium, which is reduced to its elemental form or could be integrated into selenoproteins in the form of selenocysteine. This ability to produce bioavailable organic selenium for humans is an advantage that could be exploited in the production of functional...

En esta investigación se evaluó el efecto de interacción que presentan los ácidos húmicos (AHs) con el arsénico trivalente (As III) en la formación de aglomerados en presencia de sales de metales de Fe3+ o Al3+ (Me) (AHs-Me-As); con la finalidad de eliminar al metaloide junto con la materia orgánica (MO) en sistemas de agua que es destinada al uso...

Fish proteins are a promising source for multifunctional bioactive peptide production. Thus, this work aimed to establish the potentiality of rainbow trout muscle protein for the obtention of antioxidant and antihypertensive hydrolysates. Alcalase application produced a hydrolysate with inhibition of angiotensin-converting enzyme equivalent to 56.4...

Bioactive peptides derived from diverse food proteins have been part of diverse investigations. Whey is a rich source of proteins and components related to biological activity. It is known that proteins have effects that promote health benefits. Peptides derived from whey proteins are currently widely studied. These bioactive peptides are amino aci...

In recent years, the biosynthesis of seleno amino acids from inorganic selenium has been a subject of investigation. Saccharomyces cerevisiae has been reported to bioaccumulate selenium through the metabolism of selenate. Different authors have postulated a metabolic pathway in which selenate is converted into selenomethionine or selenocysteine bas...

Brewers’ spent grain is an important source of non-starch polysaccharides, such as arabinoxylans. In this paper, a fraction rich in arabinoxylans was extracted from brewers’ spent grain (BSG-AX) by alkaline medium; was characterized by FTIR spectroscopy, scanning electronic microscopy (SEM), and zeta potential; and was used to prepare fourteen ther...

The germination of legume seeds is an effective, fast and economical technique that allows to improve the nutritional qualityof the flours obtained from them, it has an increase in the concentration of nutrients, such as proteins, fiber and vitamins, it has also been evidenced a decrease in anti-nutritional compounds such as phytic acid, lectins, s...

Health benefits of probiotics and production of inhibitors of angiotensin converting enzyme (ACE) released during milk fermentation are well known. That is why in this investigation the proteolytic profile and ACE inhibitory capacity of peptide fractions from protein hydrolysis of milk during fermentation processes was analyzed. Milk fermentation w...

β-glucans come from cereals that have been located within compounds with prebiotic activity. They have presented several bioactivities that have determined their high functional value. The aim of this study was to identify the influence of oat β-glucan on the survival and proteolytic activity of Lactobacillus rhamnosus GG in a milk fermentation thr...

Several modern drugs, which are derived from traditional herbal medicine are used in contemporary pharmacotherapy. Currently, the study of drug–plant interactions in pain has increased in recent years, looking for greater efficacy of the drug and reduce side effects. The antinociception induced by intragastric co-administration of the combination o...

Abstract: Selenium nanoparticles (SeNPs) are gaining importance in the food and medical fields due to their antibacterial properties. The microbial inhibition of these kinds of particles has been tested in a wide range of Gram (+) and Gram (−) pathogenic bacteria. When SeNPs are synthesized by biological methods, they are called biogenic SeNPs, whi...

Currently, the interest of the food industry on the use of probiotics in order to provide beneficial e�ects is increasing. However, one limitation is getting microorganisms to survive in food matrices. In this work, protective e�ect of exopolysaccharide produced by Lactobacillus delbrueckii subsp. bulgaricus NCFB 2772 on Lactobacillus rhamnosus GG...

El objetivo fue caracterizar física y químicamente el jengibre (Zingiber officinale) cultivado en la Sierra Norte de Puebla, en tres estados de madurez. Las propiedades físicas determinadas fueron eje mayor, eje menor, espesor, peso, volumen y color. La composición proximal se realizó de acuerdo a la metodología de la AOAC. El contenido de minerale...

Selenium is an essential micronutrient and has been shown to have an antioxidant effect. This can prevent different diseases such as coronary illness or cancer. Its deficiency generates neuromuscular disorders, which is why a recommended daily intake for humans of 55 μg/day has been proposed but it is not always covered. Selenium is integrated as s...

Gut microbiota is a group of microorganisms that are deposited throughout the entire gastrointestinal tract. Currently, thanks to genomic tools, studies of gut microbiota have pointed towards the understanding of the metabolism of important bacteria that are not cultivable and their relationship with human homeostasis. Alterations in the compositio...

Nowadays, there are few options of low or no added sugar drinks offering health benefits and oriented to consumers with a special health condition, such as overweight and obesity. The objective of this work was to develop and characterize a low-calorie antioxidant beverage made from aqueous extracts of medicinal plants (Melissa and lemon verbena),...

The biosynthesis of inorganic selenium into seleno amino acids has been studied in recent years. Thus, it has been reported that Saccharomyces cerevisiae bioaccumulates selenium from the metabolism of inorganic selenium. Based on the studies conducted, several authors have proposed a biotransformation metabolism of selenate into selenomethionine or...

The objective of this research was to obtain a macerate with prebiotic potential from barley straw obtained after fermentation in solid-state. Fermentation process was carried out for 7 weeks at 30 °C using sterilized barley straw inoculated with Rhizopus oryzae JCP024. Spores number, changes in pH, and carbohydrates were monitored every 24 h. Deio...

Fermented and/or protein-rich foods, the most widely consumed worldwide, are the most susceptible to the presence of high levels of biogenic amines (BAs). Many reviews have focused on BAs toxicity and presence in foods; however, technological strategies such as evaluation of physical parameters, the addition of natural or synthetic compounds or the...

The objective of this paper is to compare conventional, ultrasound, microwave, and French press methods for the extraction of antioxidant compounds from Decatropis bicolor in an aqueous medium. This plant is widely used in Mexican traditional medicine for breast cancer treatment. Despite that, there are few studies on D. bicolor. Two response surfa...

The importance of functional foods added with probiotics and prebiotics is directly related with the promotion of the health benefits of human. In this study the survival of the probiotic Lactobacillus rhamnosus GG in a semi-ripened cheese added with agave juice, was determined. Cheese ripening was carried out at 14 °C for 35 days. The pH changes w...

Amaranth is a protein-rich seed, highly consumed in Mexico and reported as a potential source of bio-peptides with physiological function. Therefore, the objective of this study was to evaluate and identify bioactive peptide sequences from amaranth protein fractions released by pepsin and pancreatin in vitro digestion. Multifunctional bioactivities...

Fucooligosaccharides are the main group into the human milk oligosaccharides. Due to their beneficial properties, a series of synthetic methods have been proposed to obtain them. In this way, the α-L-fucosidase from Thermotoga maritima has emerged as a powerful catalyst for fucooligosaccharides production. Nonetheless, the limited options in substr...

Human milk oligosaccharides (HMO) have attracted great interest in recent years due to their role in boosting infants and adults health. According to several in vitro, in vivo and clinical studies, gastrointestinal and immune physiological systems benefit the most from HMO intake. Other organ systems, such as the respiratory, central nervous, circu...

Human milk oligosaccharides (HMO) have attracted great interest in recent years due to their role in boosting infants and adults health. According to several in vitro, in vivo and clinical studies, gastrointestinal and immune physiological systems benefit the most from HMO intake. Other organ systems, such as the respiratory, central nervous, circu...

Ginger rhizome is widely used in culinary preparations and in traditional medicine. Its benefits are associated with its antioxidant properties related to phenolics and terpenoids compounds, which use to be thermolabile. Ultrasound-assisted extraction has been useful for enhancing the release of thermosensitive compounds present in vegetable tissue...

In Mexico, whey is discarded into drains without treatment, which represents an environmental problem. It is known that whey is a mixture of nutrients that increase biochemical demand (BOD) and chemical oxygen demand (COD), exceeding the limits established by national and international standards. Some uses of whey have been proposed to reduce the n...

Due to worldwide thrombosis incidence, new anticoagulant drugs have been developed. Therefore, diverse techniques are used to measure this capacity. In this sense, a size-exclusion chromatography method has already proposed, which depends on the fibrin solubilization step. Thus, the work aimed to establish the influence of different fibrin solubili...

The health benefits attributed to probiotics generate interest in the search of competent strains adapted to several ecological niches, especially those related to traditional beverages and foods of each country. Pineapple tepache, a traditional Mexican fermented beverage, was used for the isolation of lactic acid bacteria with probiotic potential,...

Chemical synthesis of carbohydrates is a challenging task. Several protection and deprotection steps of hydroxyl groups are required to ensure regioselective formation of the glycosidic bond. Usually, it is achieved through acylation, where conventional heating is combined with addition of Lewis acids as catalysts. This traditional approach has two...

Amaranthus hypochondriacus spp. is a commonly grown cereal in Latin America, known for its high protein content. The objective of this study was to separate and identify bioactive peptides found in amaranth seeds through enzymatically-assisted hydrolysis using alcalase and flavourzyme. Hydrolysis was carried out for each enzyme separately and compa...

Milk whey (MW) is a by-product of cheese factory, which causes serious environmental problems, due to is poured into the rivers, without treatment. However, MW contains high-value compounds, like lactoferrin (Lf). Several methods have been suggested to separate Lf from MW, including ultrafiltration and chromatography a�nity. In this work, extractio...

Fermentation has shown to be an effective technique in bioactive peptides release. That is why in this study antihypertensive, antithrombotic, and antioxidant activity was evaluated during amaranth proteins fermentation with Lactobacillus casei Shirota and Streptococcus thermophilus 54102 in mono and combined culture. During fermentation an increas...

Selenium is included in selenoprotein sequences, which participate in enzymatic processes necessary to preserve optimal health. Some lactic acid bacteria carry out the biotransformation of inorganic selenium in their metabolism. The complete biochemical mechanism of selenium biotransformation is still unknown; however, it is known that both the sel...

Extracts of wheat bran and barley husk with solvents of different polarity were analyzed to determine the concentration of bioactive compounds (phytic acid, saponins, flavonoids, total phenolic compounds and phenolic profile) and their antioxidant activity by DPPH and ABTS assays. The results showed that the phytic acid concentration in wheat bran...

In this study, the survival and proteolysis of lactic acid bacteria Lactobacillus casei Shirota and Lactobacillus rhamnosus GG were measured in fermented milks enriched with agave juice and inulin. Fermentations were terminated at pH 4.5 and stored for 21 days at 4 °C. Survival was determined by viable count in MRS-agar. The production of free amin...

In food systems, lipid oxidation is one of the most important factors affecting food quality, nutrition, safety, color and consumer acceptance. The objective of this study was to determine the kinetic parameters of lipid oxidation and its influence in the shelf life of third generation (3G) snacks stored in three types of packaging (polyethylene, p...

In the last decade, thrombosis has been one of the pathologies with high incidence creating great concern in Health Institutes all around the world. Considering this, the aim of this research was to determine the antithrombotic activity of peptides released during lactic fermentation. Reconstituted skim milk powder was fermented by Lactobacillus ca...

The growth of lactic acid bacteria (LAB) generates a high number of metabolites related to aromas and flavors in fermented dairy foods. These microbial proteases are involved in protein hydrolysis that produce necessary peptides for their growth,releasing different molecules of interest like bioactive peptides, during their activity. Each genus in...

Lemon balm (Melissa officinalis) is a plant in the family of Lamiaceae. In Mexican traditional medicine it is used to alleviate gastrointestinal and hepatic problems. Studies carried out mainly in ethanolic and methanolic extracts, have revealed the presence of diverse compounds to which those medicinal properties are attributed. The objective of t...

In vitro analysis of anticoagulant compounds with a potential use as antithrombotic drugs, has been traditionally performed using techniques like spectrophotometry, turbidimetry, as well as electrochemical and clinical assays. Although, these techniques have some disadvantages such as: the inability to measure the total biological activity of throm...

En el programa de la asignatura de Física y Química de superficies se contemplan los fenómenos electrocinéticos, donde figura la electroforesis; la cual es una técnica útil para caracterizar cambios atribuidos a la transformación de biomoléculas como es el caso de las proteínas. Por ello, en el presente trabajo se evaluó la modificación del perfil...

In this work, a simple method based on anthocyanins degradation by nitrite ion was used to determine cyclamate in table-top sweeteners. The method was carried out in four steps: (1) cyclamate hydrolysis (hydrolysed cyclamate, HC); (2) reaction between HC and nitrous acid; (3) anthocyanins degradation by residual nitrous acid; and (4) Absorbance mea...