Luis Borrás

Luis Borrás
University of Valencia | UV · Departament of Chemical Engineering

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54
Publications
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801
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Publications

Publications (54)
Article
Due to their capacity to assimilate carbon dioxide and nutrients, microalgae-based cultivation systems have emerged as a green solution for intensive wastewater treatment. However, when large concentrations of ammonium are present the competition between microalgae and ammonium-oxidising bacteria plays a significant role. Microalgae use ammonium to...
Article
Harvested microalgae Chlorella spp. and primary sludge were co-digested in a laboratory-scale anaerobic membrane bioreactor (AnMBR) under thermophilic conditions (55 °C). The system was run for 700 days divided into four experimental phases to determine the influence of the organic loading rate on the process performance and the microbial community...
Article
A hollow fibre membrane contactor has been applied for nitrogen recovery from anaerobic digestion supernatant at different operating conditions obtaining nitrogen recovery efficiencies over 99%. A mathematical model able to represent the time evolution of pH and nitrogen concentration during the recovery process is presented in this paper. The deve...
Article
Microalgae cultivation has been receiving increasing interest in wastewater remediation due to their ability to assimilate nutrients present in wastewater streams. In this respect, cultivating microalgae in membrane photobioreactors (MPBRs) allows decoupling the solid retention time (SRT) from the hydraulic retention time (HRT), which enables to in...
Article
Outdoor microalgae cultivation systems treating anaerobic membrane bioreactor (AnMBR) effluents usually present ammonium oxidising bacteria (AOB) competition with microalgae for ammonium uptake, which can cause nitrite accumulation. In literature, nitrite effects over microalgae have shown controversial results. The present study evaluates the nitr...
Article
Methane production from microalgae can be enhanced through anaerobic co-digestion with carbon-rich substrates and thus mitigate the inhibition risk associated with its low C:N ratio. Acclimated microbial communities for microalgae disruption can be used as a source of natural enzymes in bioenergy production. However, co-substrates with a certain mi...
Article
Ruminal fluid was inoculated in an Anaerobic Membrane Reactor (AnMBR) to produce biogas from raw Scenedesmus. This work explores the microbial ecology of the system during stable operation at different solids retention times (SRT). The 16S rRNA amplicon analysis revealed that the acclimatised community was mainly composed of Anaerolineaceae, Spiroc...
Article
Two outdoor photobioreactors were operated to evaluate the effect of variable ambient temperature on an indigenous microalgae-nitrifying bacteria culture dominated by Chlorella. Four experiments were carried out in different seasons, maintaining the temperature-controlled PBR at around 25 °C (by either heating or cooling), while the temperature in...
Article
The potential of microbial communities for efficient anaerobic conversion of raw microalgae was evaluated in this work. A long-term operated thermophilic digester was fed with three different Organic Loading Rates (OLR) (0.2, 0.3 and 0.4 g·L−1·d−1) reaching 32–41% biodegradability values. The microbial community analysis revealed a remarkable prese...
Article
The application of low ozone dosage to minimize the problems caused by filamentous foaming was evaluated in two bioreactors of an urban wastewater treatment plant. Filamentous and nitrifying bacteria, as well as protist and metazoa, were monitored throughout a one-year period by FISH and conventional microscopy to examine the effects of ozone appli...
Article
This research work proposes an innovative water resource recovery facility (WRRF) for the recovery of energy, nutrients and reclaimed water from sewage, which represents a promising approach towards enhanced circular economy scenarios. To this aim, anaerobic technology, microalgae cultivation, and membrane technology were combined in a dedicated pl...
Article
Anaerobic Membrane Bioreactors (AnMBRs) can achieve maximum energy recovery from urban wastewater (UWW) by converting influent COD into methane. The aim of this study was to assess the anaerobic biodegradability...
Article
Anaerobic digestion of indigenous Scenedesmus spp. microalgae was studied in continuous lab-scale anaerobic reactors at different temperatures (35 °C and 55 °C), and sludge retention time - SRT (50 and 70 days). Mesophilic digestion was performed in a continuous stirred-tank reactor (CSTR) and in an anaerobic membrane bioreactor (AnMBR). Mesophilic...
Article
Notorious changes in microbial communities were observed during and after the joint treatment of wastewater with Food Waste (FW) in an Anaerobic Membrane Bioreactor (AnMBR) plant. The microbial population was analysed by high-throughput sequencing of the 16S rRNA gene and dominance of Chloroflexi, Firmicutes, Synergistetes and Proteobacteria phyla...
Poster
Full-text available
Biological nitrification-denitrification is commonly used for nitrogen removal from wastewaters. Nitrification is mainly carried out by ammonia-oxidizing bacteria and archaea (AOB and AOA) and nitrite-oxidizing bacteria (NOB). They are a phylogenetically diverse guild with pronounced ecological niche specialization and they differ from each other i...
Article
BACKGROUND This work is focused on the assessment of the performance of mini‐scale air‐breathing microbial fuel cells (MFCs), by monitoring the evolution of the bio‐electrogenic activity for a period of 40 days and by comparing the microorganisms populations developed in each of the MFCs after this period. RESULTS Five MFCs were operated at sludge...
Article
In a previous study the authors confirmed the ability of PAOs to perform GAO metabolism in short-term experiments. However, what happens when PAOs are exposed to poly-P shortage for an extended period of time? The answer to this question was the aim of this work from a macroscopic and microscopic point of view. Therefore, the poly-P was removed fro...
Article
Mediante tratamientos biológicos se puede efectuar la eliminación de nutrientes nitrógeno y fósforo de las aguasresiduales. Las bacterias acumuladoras de polifosfatos (PAOs) son las responsables de la eliminación biológica de fósforo en condiciones anaerobias-aerobias/anóxicas. Estas bacterias compiten por el sustrato con las bacterias acumuladoras...
Article
The effects of two sequencing batch reactor operation strategies for phosphorus stream enrichment over the biological phosphorus removal performance have been studied. The objective of both strategies is of performing an extraction cycle in order to obtain a new stream highly enriched with phosphorus. In the 1st strategy the amount of influent vola...
Article
Full-text available
This paper deals with the effect of a bioaugmentation batch enhanced (BABE) reactor implementation in a biological nutrient removal pilot plant on the populations of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB). The results of fluorescence in situ hybridization (FISH) technique showed that AOB and NOB populations were signi...
Article
Enhanced biological phosphorus removal (EBPR) is one of the most important methods of phosphorus removal in municipal wastewater treatment plants, having been described by different modelling approaches. In this process, the PAOs (polyphosphate accumulating organisms) and GAOs (glycogen accumulating organisms) compete for volatile fatty acids uptak...
Article
Full-text available
Eight different phenotypes were studied in an activated sludge process (AeR) and anaerobic digester (AnD) in a full-scale wastewater treatment plant by means of fluorescent in situ hybridization (FISH) and automated FISH quantification software. The phenotypes were ammonia-oxidizing bacteria, nitrite-oxidizing bacteria, denitrifying bacteria, phosp...
Article
Full-text available
En el tratamiento biológico de aguas residuales participan diversos microorganismos entre los que se encuentran las bacterias filamentosas. El crecimiento excesivo e incontrolado de estos microorganismos genera problemas asociados al esponjamiento de los fangos conocido como bulking y la formación de espumas o foaming. La correcta identificación de...
Article
Full-text available
Integrated studies relating nitrifying bacteria abundance to physico-chemical and operational variables in wastewater treatment plants are scarce. The knowledge of these relationships under an integrated perspective can provide valuable information to optimize the nitrification process. The present study was carried out in a wastewater treatment pl...
Article
Full-text available
Integrated studies relating nitrifying bacteria abundance to physicochemical and operational variables in wastewater treatment plants are scarce. The knowledge of these relationships under an integrated perspective can provide valuable information to optimize the nitrification process. The present study was carried out in a wastewater treatment pla...
Conference Paper
One of the main operational problems in membranes bioreactors (MBRs) is the membrane fouling. Bacteria present in the biomass of an activated sludge system produce sticky compounds denominated extracellular polymeric substances (EPS) which result from active bacterial secretion, shed from the cell surface or cell lysis. Thus, the activated sludge f...
Poster
Full-text available
Domestic sewage should be subjected to purification processes before being discharged to streams or reused for other purposes. One of the most innovative biological treatment processes is the membrane biological reactor (MBR). MBR systems are based on the combination of the activated sludge system and membrane technologies to separate the particula...
Article
The anaerobic treatment of sulphate-rich wastewater causes sulphate reducing bacteria (SRB) and methanogenic archaea (MA) to compete for the available substrate. The outcome is lower methane yield coefficient and, therefore, a reduction in the energy recovery potential of the anaerobic treatment. Moreover, in order to assess the overall chemical ox...
Article
Previous studies have shown that polyphosphate-accumulating organisms (PAOs) are able to behave as glycogen-accumulating organisms (GAOs) under different conditions. In this study we investigated the behavior of a culture enriched with Accumulibacter at different levels of polyphosphate (poly-P) storage. The results of stoichiometric ratios Gly(deg...
Poster
Full-text available
La nitrificación está catalizada por procariotas aerobios quimilitotrófos: bacterias oxidadoras de amonio del dominio Bacteria (AOB), bacterias oxidadoras del amonio del dominio Archaea (AOA) y bacterias oxidadoras de nitrito (NOB). Hasta la fecha no se conoce ningún procariota con la capacidad de realizar los dos pasos de la nitrificación. Todas l...
Article
A continuously aerated SHARON (single reactor high activity ammonia removal over nitrite) system has been operated to achieve partial nitritation. Two sets of batch experiments were carried out to study the effect of ammonia concentration and salinity on the activity of ammonia-oxidizing bacteria (AOB). Activity of AOB raised as free ammonia concen...
Patent
The method comprises the application of molecular microbiological techniques of in situ hybridation (FISH), staining with DAPI and cell viability to determine the contribution to the chemical oxygen demand of the specific groups of bacteria present in biological waste-water purification processes (heterotrophic bacteria, autotrophic bacteria and po...
Article
In this work, the effect of calcium concentration in wastewater on the polyphosphate accumulating organisms (PAO) is investigated as well as its influence in PAO metabolism, specifically in the YPO4 (ratio between phosphorus release and acetic acid uptake). For this study a sequencing batch reactor (SBR) anaerobic-aerobic was used, in which the PAO...
Article
A glycogen nonpolyphosphate-accumulating organism (GAO) enrichment culture dominated by the Alphaproteobacteria cluster 1 Defluviicoccus was investigated to determine the metabolic pathways involved in the anaerobic formation of polyhydroxyalkanoates, carbon storage polymers important for the proliferation of microorganisms in enhanced biological p...
Article
A glycogen nonpolyphosphate-accumulating organism (GAO) enrichment culture dominated by the Alphaproteobacteria cluster 1 Defluviicoccus was investigated to determine the metabolic pathways involved in the anaerobic formation of polyhydroxyalkanoates, carbon storage polymers important for the proliferation of microorganisms in enhanced biological p...
Article
This paper focuses on the application of Kohonen self-organizing maps (SOM) and principal component analysis (PCA) to thoroughly analyse and interpret multidimensional data from a biological process. The process is aimed at enhanced biological phosphorus removal (EBPR) from wastewater. In this work, SOM and PCA are firstly applied to the data set i...
Article
A sequencing batch reactor that is operated for biological phosphorus removal has been operated under different influent calcium concentrations to study the precipitation process and the possible effects of phosphorus precipitation in the biological phosphorus removal process. Four experiments were carried out under different influent calcium conce...
Article
The aim of this paper is to study the precipitation process in a sequencing batch reactor (SBR) operated for EBPR (enhanced biological phosphorus removal) and the possible effects of this phosphorus precipitation in the biological process. Four experiments were carried out under different influent calcium concentration. The experimental results and...
Article
A sequencing batch reactor was operated in the conventional anaerobic-aerobic mode for enhanced biological phosphorus removal using acetate as the sole substrate. Despite the nutrients concentrations in the influent being high enough to satisfy the biological requirements, Zoogloea ramigera managed to grow in the system until it had negative effect...
Article
An anaerobic aerobic laboratory scale sequencing batch reactor (SBR) was operated to study the effect of pH on enhanced biological phosphorus removal. Seven steady states were achieved under different operating conditions. In all of them, a slight variation in the pH value was observed during anaerobic phase. However, pH rose significantly during a...
Article
The role of calcium (Ca) in enhanced biological phosphorus removal and its possible implications on the metabolic pathway have been studied. The experience has been carried out in an SBR under anaerobic-aerobic conditions for biological phosphorus removal during 8 months. The variations of influent Ca concentration showed a clear influence on the E...
Article
A struvite crystallisation process is highly dependent on pH. To achieve a high phosphorus recovery as struvite it is important to have an accurate control of pH in the reactor. The high non-linear response of pH value makes manual pH control difficult. Therefore, a software based on fuzzy logic control (FLC) has been developed to maintain the pH a...
Article
This paper presents an extension of the Activated Sludge Model No. 2d (ASM2d) including a chemical model able to calculate the pH value in biological processes. The developed chemical model incorporates the complete set of chemical species affecting the pH value to ASM2d describing non-equilibrium biochemical processes. It considers the system form...
Article
Full-text available
This paper presents laboratory-scale experimentation carried out to study enhanced biological phosphorus removal. Two anaerobic aerobic (A/O) sequencing batch reactors (SBR) have been operated during more than one year to investigate the information provided by monitoring pH and electric conductivity under stationary and transient conditions. Conti...

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Project (1)
Project
The LIFE MEMORY project aims to demonstrate at an industrial prototype scale an anaerobic technology using Submerged Anaerobic Membrane Bioreactor (SAnMBR) technology as an alternative to traditional urban wastewater treatment. This new approach focuses on a more sustainable concept, where wastewater turns into a source of energy and nutrients, and also a recyclable water resource by membrane disinfection. The LIFE MEMORY project has three main objectives: - Reduction of WWTPs energy consumption by 70% - Reducing by 80% of the net greenhouse gases emission per unit of COD removed from the influent wastewater, avoiding the oxidation of organic matter - Increase effluent quality for reuse and minimization of residuals by 50%