Luigina Di Gialleonardo

IZS Istituto Zooprofilattico Sperimentale | IZS · Department of Vaccine Research and Development

In this report, we describe eight complete genome sequences of African horse sickness virus (AHSV) strains belonging to four different serotypes, namely, AHSV-5, AHSV-6, AHSV-8, and AHSV-9. Samples were collected in Namibia and South Africa from infected horses between 2000 and 2011. As expected, phylogenetic analyses of the variable outer capsid p...

Cat scratch disease (CSD) is a zoonotic disease, caused predominantly by Bartonella henselae and transmitted to humans through a scratch or bite of the cat. Cat represents the principal reservoir and healthy carrier of Bartonella, which is mainly transmitted, among cats, by the flea Ctenocephalides felis. During 2014, fifty‑two samples of whole blo...

Background: As the epidemiology of human Q Fever generally reflects the spread of Coxiella burnetii in ruminant livestock, molecular characterization of strains is essential to prevent human outbreaks. In this study we report the genetic diversity of C. burnetii in central Italy accomplished by MST and MLVA-6 on biological samples from 20 goat, sh...

Importance: Bluetongue is one of the main infectious diseases of ruminants and is caused by bluetongue virus (BTV), an arthropod-borne virus transmitted from infected to susceptible animals byCulicoidesbiting midges. Bluetongue has a variable clinical outcome that can be related to both virus and host factors. It is therefore critical to understan...

After a May 2011 outbreak of Rift Valley fever among livestock northeast of Etosha National Park, Namibia, wild ruminants in the park were tested for the virus. Antibodies were detected in springbok, wildebeest, and black-faced impala, and viral RNA was detected in springbok. Seroprevalence was high, and immune response was long lasting.

Unlabelled: Bluetongue virus (BTV) causes bluetongue, a major hemorrhagic disease of ruminants. In order to investigate the molecular determinants of BTV virulence, we used a BTV8 strain minimally passaged in tissue culture (termed BTV8L in this study) and a derivative strain passaged extensively in tissue culture (BTV8H) in in vitro and in vivo s...

Unlabelled: Bluetongue is a major infectious disease of ruminants caused by bluetongue virus (BTV), an arbovirus transmitted by Culicoides. Here, we assessed virus and host factors influencing the clinical outcome of BTV infection using a single experimental framework. We investigated how mammalian host species, breed, age, BTV serotypes, and stra...

GenBank Accession Numbers KM053268-KM053277 represent sequences from the 10 segments of Bluetongue virus 2 strain BTV-2IT2000.

GenBank Accession Numbers KM053268-KM053277 represent sequences from the 10 segments of Bluetongue virus 2 strain BTV-2IT2000.

GenBank Accession Numbers KM053268-KM053277 represent sequences from the 10 segments of Bluetongue virus 2 strain BTV-2IT2000.

GenBank Accession Numbers KM053268-KM053277 represent sequences from the 10 segments of Bluetongue virus 2 strain BTV-2IT2000.

GenBank Accession Numbers KM053268-KM053277 represent sequences from the 10 segments of Bluetongue virus 2 strain BTV-2IT2000.

GenBank Accession Numbers KM053268-KM053277 represent sequences from the 10 segments of Bluetongue virus 2 strain BTV-2IT2000.

GenBank Accession Numbers KM053268-KM053277 represent sequences from the 10 segments of Bluetongue virus 2 strain BTV-2IT2000.

GenBank Accession Numbers KM053268-KM053277 represent sequences from the 10 segments of Bluetongue virus 2 strain BTV-2IT2000.

GenBank Accession Numbers KM053268-KM053277 represent sequences from the 10 segments of Bluetongue virus 2 strain BTV-2IT2000.

GenBank Accession Numbers KM053268-KM053277 represent sequences from the 10 segments of Bluetongue virus 2 strain BTV-2IT2000.

GenBank Accession Numbers KM053258-KM053267 represent sequences from the 10 segments of Bluetongue virus 8 strain BTV-8IT2008.

GenBank Accession Numbers KM053258-KM053267 represent sequences from the 10 segments of Bluetongue virus 8 strain BTV-8IT2008.

GenBank Accession Numbers KM053258-KM053267 represent sequences from the 10 segments of Bluetongue virus 8 strain BTV-8IT2008.

GenBank Accession Numbers KM053258-KM053267 represent sequences from the 10 segments of Bluetongue virus 8 strain BTV-8IT2008.

GenBank Accession Numbers KM053258-KM053267 represent sequences from the 10 segments of Bluetongue virus 8 strain BTV-8IT2008.

GenBank Accession Numbers KM053258-KM053267 represent sequences from the 10 segments of Bluetongue virus 8 strain BTV-8IT2008.

GenBank Accession Numbers KM053258-KM053267 represent sequences from the 10 segments of Bluetongue virus 8 strain BTV-8IT2008.

GenBank Accession Numbers KM053258-KM053267 represent sequences from the 10 segments of Bluetongue virus 8 strain BTV-8IT2008.

GenBank Accession Numbers KM053258-KM053267 represent sequences from the 10 segments of Bluetongue virus 8 strain BTV-8IT2008.

GenBank Accession Numbers KM053258-KM053267 represent sequences from the 10 segments of Bluetongue virus 8 strain BTV-8IT2008.

Understanding the factors governing host species barriers to virus transmission has added significantly to our appreciation of virus pathogenesis. Jaagsiekte sheep retrovirus (JSRV) is the causative agent of ovine pulmonary adenocarcinoma (OPA), a transmissible lung cancer of sheep that has been rarely described in goats. In this study, in order to...

Bluetongue is a major infectious disease of ruminants that is caused by bluetongue virus (BTV). In this study, we analyzed virulence and genetic differences of (i) three BTV field strains from Italy maintained at either a low (L strains) or high (H strains) passage number in cell culture and (ii) three South African "reference" wild-type strains an...

A two year study (2008-2009) was carried out to monitor the Usutu virus (USUV) circulation in Italy. Sentinel horses and chickens, wild birds and mosquitoes were sampled and tested for the presence of USUV and USUV antibodies within the WND National Surveillance plan. Seroconversion evidenced in sentinel animals proved that in these two years the v...

Four groups of BTV free Frisian and cross bred calves were used to determine the length of viraemia following infection with different doses of BTV-8 Italian isolate. The first group of five animals was infected with 10 TCID(50) of BTV-8, the second group of four animals with 10(3) TCID(50) and the third group, which also included four animals, was...

In the 2004, the Sardinian bluetongue (BT) vaccination campaign used the combination of monovalent BTV-2, BTV-4 and BTV-16 modified-live vaccines manufactured by the Onderstepoort Biological Products in South Africa. Following vaccination, some herds showed temperature, oedema, lameness, hyperaemia and decrease in milk production, and some others r...

This study described the first report of BTV-16 in Croatia. Serological evidence occurred in cattle at the end of September and continued during October and November 2004. All positive animals were in the Dubrovnik-Neretva County, a region located in the southernmost part of Croatia. BTV-16 infection was also detected in goats and sheep. Apart from...

In the recent years, USUTU virus (USUV), a flavivirus of the Japanese encephalitis virus complex, has been reported in Central Europe. As part of a systematic surveillance programme to monitor possible entrance and/or circulation of vector-borne viruses, since 2001, sentinel-chicken flocks were placed throughout the Italian territory nearby areas c...

Since 2000 severe, long-lasting epidemics of bluetongue virus (BTV) have been described in Italy, caused by BTV serotypes 2, 4, 9 and 16. Vaccination programs have been applied extensively to control the infection, in spite of concerns about the potential dissemination of attenuated vaccine strains of BTV in susceptible animals. Accordingly, rapid...

The authors describe a real-time reverse transcriptase polymerase chain reaction (RT-PCR) to detect bluetongue viruses (BTV) in blood samples. The primers and Taqman probes used were specific for a conserved region of BTV RNA segment 5, which encodes non-structural protein NS1. The method was able to detect strains of BTV serotypes 2, 4, 9 and 16 i...

Riassunto In questo lavoro è descritto un metodo RT-PCR real-time per rilevare il virus della bluetongue (BTV) in campioni di sangue. Sono stati utilizzati primer e TaqMan probe specifici per una regione conservata dell'RNA di BTV localizzata sul segmento 5 che codifica per la proteina non strutturale NS1. Il metodo è stato in grado di rilevare vir...