Luigi Pane

Luigi Pane
Università degli Studi di Genova | UNIGE · Dipartimento di Scienze della terra, dell'ambiente e della vita (DISTAV)

PhD

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85
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Publications

Publications (85)
Article
Microplastics are nowadays recognized as one of the most important pollution sources in the aquatic environment. Their presence has been investigated and certified in various parts of the globe and, most importantly, in a wide variety of species belonging to different trophic levels. However, the process of microplastics vertical distribution among...
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Distributional data on planktic copepods (Crustacea, Copepoda) collected in the framework of the III rd , V th , and X th Expeditions of the Italian National Antarctic Program (PNRA) to the Ross Sea sector from 1987 to 1995 are here provided. Sampling was performed with BIONESS and WP2 nets at 94 sampling stations at depths of 0-1,000 m, with a spe...
Article
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Tigriopus fulvus (Fischer, 1860) is a benthic harpacticoid copepod of the Mediterranean supralittoral zone. The transitional characteristics of this environment forced this species to develop high resistance to changes of environmental parameters. Nevertheless, Tigriopus fulvus life-cycle is influenced from the splashpools physical-chemical paramet...
Article
The ingestion and retention of microplastics of filter-feeder organisms represent a risk for the final consumers and the environment. Biomonitoring is necessary to deal with the effects of plastic material pollution. The selection of the monitored organisms strongly affects the relevance of the results and the understanding of the environmental con...
Conference Paper
Tigriopus fulvus (Fischer, 1860) is a benthic harpacticoid copepod of the supralittoral environment, the study subject population is currently resident in Genova Nervi rockpools (Ligurian Sea, 44°22'52.561 N; 9°2'12.570 E). Main environmental rockpools parameters were studied from March To September 2014. Salinity, temperature, pH, density, copepod...
Conference Paper
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Crassostrea gigas, conosciuta anche come Ostrica concava o Ostrica giapponese è la specie di Ostreidae più commerciata al mondo tanto da rappresentare, al 2012, il 75% della produzione europea di settore (FAP, 2012). Originaria del Pacifico, essa è considerata ora una specie alloctona naturalizzata nel Mar Mediterraneo, nel Golfo di La Spezia ne è...
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Recurrent outbreaks of Pelagia noctiluca and health problems consequent to stings were recorded during the last decades. This phenomenon forced some Italian University laboratories to study this cnidarian. The first studies concerned the distribution, biochemical composition and morphology of nematocysts of Pelagia noctiluca. The discharge mechanis...
Article
Surfactants are synthetic chemicals utilized as detergents and cleaning products or as dispersants and emulsifiers to face water pollution. In spite of this, due to their wide diffusion, surfactants can induce water and soil pollution, notably in developed countries, and can be toxic to organisms. Taking into account that the assessment of new comp...
Article
Cnidarians are numbered among the most venomous organisms. Their venoms are contained in intracellular capsules, nematocysts, which inject the content into preys/attackers through an eversion system resembling a syringe needle. Several cnidarian venoms have activity against the nervous system, being neurotoxic, or affect other systems whose functio...
Conference Paper
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Tigriopus fulvus (Fischer, 1860) is a supralittoral harpacticoid copepod usually found in Mediterranean Sea splashpools which has many biological applications (aquaculture, ecotoxicology, including genomics). Aim of the present paper is to contribute to the ecological knowledge of the Tigriopus fulvus populations of Genova Nervi studying the main e...
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Histological, scanning electron microscopic and immunohistochemical studies were performed on the gills of juvenile gilthead (Sparus aurata L.) exposed to various concentrations of three anionic detergents: sodium dodecyl sulphate (SDS), safety sea cleaner 2 (SSC2), and FINASOL OSR2, in short term (96 h) and chronic (28 days) experiments. The morta...
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Several microorganisms – epibionts – can adhere to living supports taking advantage for their survival, feeding and movement. Epibiosis occurs particularly in aquatic environments, on both benthic and planktonic organisms, among which copepods and cladocerans represent an important living support. The harpacticoid copepod Tigriopus fulvus, living i...
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In the framework of the Risk Assessment procedure for the ecotoxicological evaluation of compounds used in the aquatic environment, tests with target species must be carried out. This paper reports the results concerning the evaluation of the acute toxicity of F-500 micelle encapsulator (Hazard Control Technologies Inc., Fayetteville, GA, USA) on c...
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The toxicity of Cnidaria is a subject of concern for its influence on human activities and public health. During the last decades, the mechanisms of cell injury caused by cnidarian venoms have been studied utilizing extracts from several Cnidaria that have been tested in order to evaluate some fundamental parameters, such as the activity on cell su...
Article
Many organisms produce bioactive substances used in the production of drugs. In this context, Cnidaria occupies a major position; for this reason, research on new bioactive substances has focused upon them as an interesting target. As a matter of fact, substances and extracts able to fight human diseases have been found in cnidarians, several of wh...
Article
Organophosphorus pesticides (OP) are dangerous xenobiotics for aquatic environments mainly because they can interact with all links of the food web inducing bioaccumulation and bioconcentration in organisms. In addition, they have an influence on the enzymes of the cholinergic system (acetylcholinesterase and pseudocholinesterase) of the organisms...
Article
Vibrio cholerae N-acetyl glucosamine-binding protein A (GbpA) is a chitin binding protein and a virulence factor involved in the colonization of human intestine. We investigated the distribution and genetic variations of gbpA in 488 V. cholerae strains of environmental and clinical origin, belonging to different serogroups and biotypes. We found th...
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Vibrio cholerae N-acetyl glucosamine-binding protein A (GbpA) is a chitin binding protein and a virulence factor involved in the colonization of human intestine. We investigated the distribution and genetic variations of gbpA in 488 V. cholerae strains of environmental and clinical origin, belonging to different serogroups and biotypes. We found th...
Article
Marine sediments are a reservoir of organic and inorganic pollutants, that can be adsorbed in suspended particulate matter and can occur in higher concentration in whole sediments than in the water column; so, the evaluation of sediment ecotoxicity provides for general information on pollutant bioavailability. Microalgae are widely employed in ecot...
Article
In this work, we investigated the efficacy of three new biocides (77351, 73532, 73503--NALCO®) as specific antifouling products against adult organisms of the bivalve Brachidontes pharaonis (Fischer P., 1870), a Lessepsian species introduced in the Mediterranean Sea by sea transport (ballast water), and which has recently shown invasive behaviour i...
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Marine sediments play an important role in the accumulation-storage and/or release of contaminants in seawaters; sedimets bioassays provide for general information on pollutant bioavailability. This work points out the importance to utilize free and immobilised (Na-alginate) marine algae for the ecotoxicological evaluation of metals (Cd, Zn, Cu) as...
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The effects of the organophosphorus pesticide Malathion, Pestanal® on the activity of the enzymes Acetylcholinesterase (AChE) and Glutathione-S-transferase and on survival were evaluated in Tigriopus fulvus. The mortality of the organisms after 96 hours (acute toxicity) and after 7 days (chronic toxicity) of treatment was evaluated. LC50 was not ca...
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The Scyphomedusa Pelagia noctiluca is known to be an harmful species able to cause contact dermatitis and also systemic symptoms in sensitive subjects. Taking into account that some compounds are known to be protective agents against jellyfish venoms, in this research the protection of non-cytotoxic zinc sulfate concentrations was evaluated on cult...
Article
Marine rocky substrata consist of different levels according to spatial location, environmental characteristics and occurrence of vegetal and animal communities. Particularly, the littoral system is divided into the supralittoral, midlittoral, infralittoral and circalittoral zones. The rocky shore is a transitional environment between the marine an...
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The Ligurian Sea is a deep basin in the northernmost sector of the western Mediterranean which shows peculiar hydrodynamic and meteo-oceanographic features. The coasts of the Ligurian Sea are among the most urbanised and industrialised along the Italian coastline: the main causes of disturbance being littoral urban development and harbour activitie...
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The production of natural toxins is an interesting aspect, which characterizes the physiology and the ecology of a number of marine species that use them for defence/offence purposes. Cnidarians are of particular concern from this point of view; their venoms are contained in specialized structures--the nematocysts--which, after mechanical or chemic...
Article
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Abstract Cnidarians exert a meaningful influence on human activities and healyh being able to produce several local and systemic symptoms on humans. Mediterranean jellyfish are scarcely poisonous, but during outbreaks the impact of some of them, such as the Scyphomedusa Pelagia noctiluca, is noteworthy. As some compounds containing lanthanum ions c...
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The correct identification of the species of origin in cell culture procedures as well as the detection of eventual cross-contamination by other cells are of essential importance to ensure the correctness of all experimental trials. In this connection, PCR is a fundamental technique to identify the occurrence of genetic information belonging to a d...
Article
The project is conducted by the VibrioSea consortium including the following institutions and leading researchers: CNES (Murielle Lafaye), MEDIAS (Jean Pierre Lacaux), CLS (Jacques Stump) and IFREMER (Dominique Hervio-Hearth) from France, University of Verona (Maria del Mar Lleò), University of Genova (Carla Pruzzo) and ISMAR-CNR Venezia (Giorgio S...
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Biotoxin production is a characterizing aspect of the physiology and ecology of several marine organisms. In this study the cytotoxic effect of crude extracts from jellyfish Pelagia noctiluca, Chrysaora hysoscella and Aurelia aurita (Cnidaria: Scyphozoa) against cultured human keratinocytes NCTC 2544 was evaluated by 3- (4,5-Dimethylthiazol-2-yl)-2...
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Full-text available
Biotoxin production is a characterizing aspect of the physiology and ecology of several marine organisms. In this study the cytotoxic effect of crude extracts from jellyfish Pelagia noctiluca, Chrysaora hysoscella and Aurelia aurita (Cnidaria: Scyphozoa) against cultured human keratinocytes NCTC 2544 was evaluated by 3- (4,5-Dimethylthiazol-2-yl)-2...
Article
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The luminescence is a feature of several marine planktonic organisms, such as bacteria, algae, cnidaria, ctenophores, crustaceans and pyrosomes, in particular, alot of pelagic crustaces have got luminescent organs (Ghirendelli, 1981, Gruner, 1994) useful for trophic, reproductive or defense purposes. Several calanoid copepods are luminescent and po...
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The toxicity of Cnidaria is a subject of concern due to its influence on humans. In particular, jellyfish blooms can highly affect human economical activities, such as bathing, fishery, tourism, etc., as well as the public health. Stinging structures of Cnidaria (nematocysts) produce remarkable effects on human skin, such as erythema, swelling, bur...
Article
Full-text available
The toxicity of Cnidaria is a subject of concern due to its influence on humans. In particular, jellyfish blooms can highly affect human economical activities, such as bathing, fishery, tourism, etc., as well as the public health. Stinging structures of Cnidaria (nematocysts) produce remarkable effects on human skin, such as erythema, swelling, bur...
Article
The uptake of cadmium and zinc by Spirulina platensis was investigated using a laboratory culture of this cyanobacterium. The cells were treated with metal concentrations increasing from 0.5 to 2.0 mg L(-1), in order to evaluate their adsorption capacity and survival potential. Afterwards, the cytotoxicity of cell extracts bioaccumulating heavy met...
Article
Background, Aim and ScopeThe toxicity of contaminated sediments should be evaluated considering the direct exposure of laboratory organisms to whole sediments and the indirect exposure to elutriates or extracts (Tay et al. 1992, Byrne and Halloran 1999, Nendza 2002). The alga Dunaliella tertiolecta is indicated for the use in toxicity bioassays bec...
Chapter
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Heavy metal pollution is a growing environmental problem in marine coastal waters;for this reason the monitoring of water pollution needs the availability of test-species ableto furnish reliable and cheap results. From this point of view, copepods are important testorganisms owing to their wide distribution and for their key-position within the foo...
Article
Full-text available
Surfactants are synthetic organic chemicals used as detergents and household cleaning products and employed in several industrial processes, such as in food, mining, oil and textile. To evaluate the toxicity of these compounds freshwater and marine crustaceans have been widely used; in particular, copepods seem to have a satisfactory sensitivity to...
Article
In order to verify the intra- and interlaboratory variability of the test, 7 laboratories conducted 48h acute bioassays exposing 50 days old seabasses (Dicentrarchus labrax, L. 1758) (Teleostei: Serranidae) (L.S. 12.11±1.44 mm) to the toxicant sodium dodecylsulfate. The average LC50 was 2.93±0.52 mg/l to 24h and 2.90±0.50 mg/l to 48h, respectively....
Article
Culturable vibrios were isolated from water and plankton fractions collected during an 18-month sampling study performed along the north-central coast of the Adriatic Sea (Italy). Unculturable Vibrio vulnificus and V. parahaemolyticus were detected in plankton fractions by polymerase chain reaction amplification of DNA sequences for cytotoxin-haemo...
Article
Marine species carried by the ships’ ballast waters are a potentially serious environmental problem. Many strategies are being adopted to minimize the transfer of invasive or pathogenic marine species between different aquatic ecosystems. This problem is often addressed by using biocides for ballast water treatment; however, the biocide could be da...
Article
From April 1996 through March 1997, culturable vibrios associated with both large (>200 μm) and small (0.45–200 μm) size plankton collected in the Ancona, Italy, coastal zone of the Adriatic Sea were studied. Vibrio alginolyticus was readily cultured from large size plankton from July to September and represented the only Vibrio species that could...
Article
Polycyclic aromatic hydrocarbons (PAHs) are widespread carcinogenic, mutagenic and teratogenic pollutants that can significantly affect ecosystems and exert an influence on marine planktonic and benthic communities as well as on higher organisms such as fish and mammals, including humans. In this paper the concentrations of six PAHs in water, sesto...
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Helicobacter pylori was isolated from marine zooplankton, and characterized by standard microbiological tests, by PCR amplification of vacA and cagA gene fragments, and by comparative sequence analysis of the vacA PCR product. In a viable-but-non-culturable (VBNC) state, this isolate, as well as the reference strain H. pylori ATCC 43629, could be r...
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The aim of this study was to investigate the in vitro cytotoxicity of 5 ceramic materials for metal-free fixed prosthodontics: In-Ceram, Cergo, IPS Empress II, Cercon ZrO2, Finesse All Ceram compared each other and to commercially pure Titanium (CpTi). The materials, prepared directly from manufacturers as 10 mm diameter and 3 mm thickness disks, h...
Article
Harbours are characterized by high pollutant charge and by the occurrence of well adapted and resistant species. This paper reports the results of an annual plankton survey (May 1997-June 1998) carried out in the western harbour of Genova (Ligurian Sea) and in its mouth. Plankton samples were collected by horizontal trawls using a WP2 net. Copepods...
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The presence of enterococci in lake and seawater in an 18-month survey comparing molecular (PCR and quantitative PCR) and culture methods was evaluated, as well as the possibility that zooplankton could act as reservoirs for enterococci. Samples of both water and zooplankton were collected monthly from a Lake Garda site and an Adriatic Sea site. In...
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The structure of the zooplankton biotic community and of copepod population in the coastal area of Terra Nova Bay (Ross Sea, Antarctica) was investigated during the 10th Italian Antarctic Expedition (1994/1995). Zooplankton biotic community consisted mainly of pteropods (Limacina helicina and Clione antarctica), Cyclopoid (Oithona similis), Poecilo...
Article
The effect of exposure to artificial sea water (ASW) on the ability of classical Vibrio cholerae O1 cells to interact with chitin-containing substrates and human intestinal cells was studied. Incubation of vibrios in ASW at 5 degrees C and 18 degrees C resulted in two kinds of cell responses: the viable but non-culturable (VBNC) state (i.e. <0.1 co...
Article
In aquatic environments heterotrophic flagellates are an important component within the microbial loop and the food web, owing to their involvement in the energy transfer and flux and as an intermediate link between bacteria and primary producers, and greater organisms, such as other protists and metazoan consumers. In the microbial loop heterotrop...
Article
Marine microalgae represent an essential link in the planktonic food web and some of them are commonly utilised in aquaculture systems as food for larval and juvenile stages of fishes, crustacea and mollusca. However, the caloric content and the biochemical composition of these microrganisms vary in relation to ageing and to several environmental c...
Article
In the present study the mercury concentration has been investigated in three components of a rock pool environment of Genoa Nervi (Ligurian Sea): water, particulate matter and Tigriopus fulvus (Harpacticoid copepod). The influence of some parameters (pH, temperature, salinity, rainfall) on mercury concentration has been evaluated. These data show...
Article
In this paper, the growth of the marine microalga Tetraselmis suecica was investigated using thermogravimetry (TG) and differential thermal analysis (DTA) to determine suitable diets for larval and juvenile development in aquaculture systems. Microalgae were maintained in synthetic sea water (19@1000 salinity, 18 degrees C constant temperature) and...
Article
In the marine environment associations between picoplankton and copepods are very important not only for the lifestyle of these animals, but also for marine ecosystems and human health. In the present study we investigated the biofouling of Tigriopus fulvus in culture and possible effects of this colonization by means of scanning electron microscop...
Chapter
Taxonomic studies, as well as density and distribution patterns of larval crustacean decapods collected during the Italian National Antarctic Expeditions in 1987–88 and 1989–90 were carried out. Specimens of Acanthephyra pelagica (Risso 1816) (Oplophoridae), Chorismus antarcticus (Pfeffer 1887) (Hippolythidae) and Notocrangon antarcticus (Pfeffer 1...
Chapter
This study presents the results obtained on copepods collected during the Italian oceanographic Antarctic expeditions in 1987–88 and 1989–90. The structure of copepod populations in Terra Nova Bay and its neighbouring Ross Sea zones was examined with particular attention to distribution and abundance of the large-sized species dominating the copepo...
Article
Chitin is one of the most abundant organic compounds in nature; in the aquatic environment it is heavily colonized by bacteria (mainly vibrios) that are responsible for its mineralization. Zooplankton organisms (e.g copepods) are known to support a significant number of attached bacteria on their chitinaceous surface. As a consequence of these inte...
Article
The growth responses of free and immobolized cultures of the marine alga Tetraselmis suecica (Chlorophyta, Prasinophyceae) were compared. Algal cells were immobilized in 2% calcium alginate solution; both entrapped and free cultures were maintained in synthetic sea water at 19 salinity and 14C constant temperature. Algal growth was evaluated by cel...
Article
As carotenoids have important biological functions, it is important to discover new natural sources of these pigments. The bacterial strains isolated from a sea water rock pool were cultivated on marine agar containing yeast extract and identified by conventional methods. The bacterial pigments were extracted with methanol and analyzed by reversed-...
Article
Vibrio alginolyticus is the only culturable vibrio associated with the chitinaceous carapace of the copepod Tigriopus fulvus (Fisher 1860) living in Ligurian coastal rock pools (Tyrrhenian Sea). The characteristics of the interaction between chitin particles and V. alginolyticus were studied by analysing strains isolated both from the copepod surfa...
Article
In aquaculture, the availability of highly nutritive and inexpensive live food is a subject of major concern; from this point of view, some harpacticoid copepods (gen. Tigriopus) are suitable, owing to both the ease of finding in nature and their constant reproduction in culture. In order to optimize the production of nauplii in Tigriopus fulvus Fi...
Article
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A study of heterotrophic bacteria and vibrios adhering to the copepod Tigriopus fulvus, which lives in Ligurian coast rock pools (Tyrrhenian Sea), was carried out from November 1990 to October 1991. Heterotrophic bacteria, which were always found both free in the water and bound to the T. fulvus organisms, showed a correlation with water temperatur...
Chapter
Results are presented from samples collected from May to September 1989 in order to study the biotic plankton community in a sea zone of the Gulf of Genoa (Ligurian Sea). Three fixed stations affected by discharges from purifying installations and river flow were considered. The samplings were supported by hydrological data (temperature, salinity,...
Article
Between March and June 1989 zooplankton and sea-water samples were taken in three different areas of Riva Trigoso Bay (eastern Ligurian Sea) every two weeks. The analysis of eight trace elements (Cd, Co, Cr, Cu, Fe, Ni, V, Zn) was conducted by means of a graphite furnace atomic absorption spectrophotometer (GFAAS). Biological material values result...
Article
The study concerns the Tigriopus fulvus specimens fatty acid composition, sampled in Ligurian coast rockpools from September 1980 to June 1981. By the study of frequency distribution it was observed that the fatty acid found in greater quantity was the palmitic one, followed by the palmitoleic, the oleic and stearic acids. Short and long chain fatt...