Lucinda Puebla-Clark- Ph.D.
- PostDoc Position at The University of Texas Medical Branch at Galveston
Lucinda Puebla-Clark
- Ph.D.
- PostDoc Position at The University of Texas Medical Branch at Galveston
About
16
Publications
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Introduction
Lucinda Puebla currently works at the University of Texas Medical Branch, macrophage response to tuberculosis
Current institution
Publications
Publications (16)
SARS-CoV-2 is a highly transmissible virus that causes COVID-19 disease. Mechanisms of viral pathogenesis include excessive inflammation and viral-induced cell death, resulting in tissue damage. Here we show that the host E3-ubiquitin ligase TRIM7 acts as an inhibitor of apoptosis and SARS-CoV-2 replication via ubiquitination of the viral membrane...
SARS-CoV-2 is a highly transmissible virus that causes COVID-19 disease. Mechanisms of viral pathogenesis include excessive inflammation and viral-induced cell death, resulting in tissue damage. We identified the host E3-ubiquitin ligase TRIM7 as an inhibitor of apoptosis and SARS-CoV-2 replication via ubiquitination of the viral membrane (M) prote...
The C-type lectin receptor (CLR) family plays an important role in mediating the innate immune responses to some viruses. The Macrophage galactose-type lectin (MGL), or CLEC10, is a CLR that binds SARS-CoV-2 Spike. This binding event is dispensable for virus entry but has been implicated in an increased inflammatory response. Downstream immune resp...
Macrophages (Mφ) are the primary replicative niche for Mycobacterium tuberculosis (Mtb), a prominent bacterial pathogen responsible for nearly 1.5 million deaths annually. Appropriate metabolic reprogramming and activation of these cells is a major determinant of host-pathogen interactions and control of infection. Macrophage galactose-type lectin...
Tuberculosis (TB) recurrence following drug treatment and apparent clinical cure affects an estimated 700,000 people annually and is increasingly associated with relapse of initial Mycobacterium tuberculosis (Mtb) infection. Cellular immunity by T helper (Th) cells, including Th17 populations is important during active TB. The contribution of Th17...
Clearance of infection with Plasmodium spp. requires both antibody and Th1-type responses. While Th1-like cells control the peak in P. chabaudi infection, however specific IgG is delayed. Both Tfh and GC-Tfh can promote specific antibodies and long-lived plasma cells to fully clear infection. GC-Tfh numbers remain stable throughout P. chabaudi infe...
Severe COVID-19 is due to a dysregulated immune response; however, the mechanisms preventing recovery of airway function are unclear. Cytokines regulate the quality of inflammation, and drive both anti-viral and disease tolerance mechanisms. There was a mostly monocytic infiltration in the lungs with alveolar thickening and consolidation 3 days pos...
Germinal center (GC) formation in P. chabaudi rodent malaria is delayed for three weeks, which can prolong the infection. T follicular helper (Tfh) cells express CXCR5 for homing to the B cell area, and IL-21 which promotes antibody production. T helper type 1 (Th1) cells express CXCR3 and IFN-γ to promote phagocytosis. Previous data suggests that...
Cytokines, such as type-I Interferons (IFN-I), are essential to mount an effective antiviral response. The production of IFN-I is regulated by multiple mechanisms, including ubiquitination of host signaling factors. However, viruses can hijack the ubiquitin (Ub) system to promote viral replication. For example, we recently reported that the E3-Ub l...
In recent years, porcine dendritic cells (DCs) have been identified from pig tissues. However, studying the interaction of porcine DCs with pathogens is still difficult due to the scarcity of DCs in tissues. In the present work, the Flt3-ligand (Flt3L)-based in vitro derivation system was further characterized and compared with other cytokine deriv...
Hybrid Th1/Tfh cells (IFN-γ⁺IL-21⁺CXCR5⁺) predominate in response to several persistent infections. In Plasmodium chabaudi infection, IFN-γ⁺ T cells controls parasitemia, while antibody and IL-21⁺Bcl6⁺ T cells effect final clearance, suggesting an evolutionary driver for the hybrid population. We found that CD4-intrinsic Bcl6, Blimp-1 and STAT3 coo...
Hybrid Th1/Tfh cells (IFN-γ+IL-21+CXCR5+) predominate in response to persistent infections. However, it is not clear if the function of these cells is plastic and might be modulated. In infection with Plasmodium spp, an IFN-γ+ T helper-1 (Th1) response controls initial parasitemia, while antibody and IL-21+CXCR5+ T follicular helper (Tfh) function...
Objective
The present work aims to develop pig conventional DC (cDC) using a Flt3L-dependent
bone marrow (BM) culture system and assess their susceptibility to PRRSV1.
Methods
BM were cultured with human Flt3L (20 ng/ml) for 14 days with half of the medium
changed every 3 days. The resulting cultures (Flt3L-DC) were characterized by flow
cytometry...
Porcine reproductive and respiratory syndrome virus (PRRSV) infects monocyte-derived DCs, and previous reports have shown that PRRSV does not infect conventional DCs (cDCs) in vitro, but the effects on cDCs from lymphoid tissues are unknown. This study analyzed the response and susceptibility of tonsil DEC205+cDCs from infected pigs. We confirmed t...
Introduction: Giardia lamblia, a protozoan intestinal parasite is able to evade or suppress defense mechanisms, such as innate response. Antigen presenting cells (APC) like dendritic cells may orchestrate an immune response and support a more effective adaptive defense. Conjugated linoleic acid (CLA), a dietary lipid, has shown biological activity...
Conventional dendritic cells (cDCs) are divided into the following different subtypes: cDC1, which promotes a Th1 response, and cDC2, which stimulates a Th2 and Th17 response. These cells have not been characterized in porcine lymphoid tissues. DEC205 is a receptor that increases antigen presentation and allows DCs to cross-present antigens. The ob...
Questions
Question (1)
I am stimulating with IL-27, IL-6, or IL-21 and trying to see pSTAT3 in naive CD4+ T cells. I have tried BD's protocol using the Perm buffer III, I have used Stumhofer et al., 2007, AOH Hall et al., 2012 and a combination of the 3 of them, I have also tried the Ab in different colors. I am incubating with complete IMDM media (done it in plates or eppendorf) for short periods of time (5, 15, 20, 30 min), with or without TCR stimulation, I have fixed at 37°C and -20°C, permeabilized with 90% methanol 30min on ice, or -20°C overnight, or using the Perm III buffer. I appreciate any tips you can give me, thank you.
