Lucile Durand

Lucile Durand
Institut Français de Recherche pour l'Exploitation de la Mer | Ifremer · Department of Deep-Sea Ecosystems

PhD

About

98
Publications
3,922
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493
Citations
Citations since 2016
39 Research Items
359 Citations
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20162017201820192020202120220204060
20162017201820192020202120220204060
20162017201820192020202120220204060

Publications

Publications (98)
Article
Full-text available
Background In deep-sea hydrothermal vent areas, deprived of light, most animals rely on chemosynthetic symbionts for their nutrition. These symbionts may be located on their cuticle, inside modified organs, or in specialized cells. Nonetheless, many of these animals have an open and functional digestive tract. The vent shrimp Rimicaris exoculata is...
Article
The herbicide glyphosate has been widely used in the past 40 years, under the assumption that side effects were minimal. In recent years, its impact on microbial compositions and potential indirect effects on plant, animal and human health have been strongly suspected. Glyphosate and co-formulates have been detected in various water sources, but ou...
Article
Full-text available
The shrimp Rimicaris exoculata and Rimicaris chacei are visually dominant fauna co-occurring at deep-sea hydrothermal sites of the Mid-Atlantic Ridge (MAR). Their co-existence was related to contrasted life-history traits, including differences in their diet and reliance on chemoautotrophic symbionts at the adult stage. Both species of shrimp are c...
Preprint
Full-text available
The shrimps Rimicaris exoculata and Rimicaris chacei are visually dominant fauna co-occurring at deep-sea hydrothermal sites of the Mid-Atlantic Ridge (MAR). Their co-existence was related to contrasted life-history traits, among which differences in their diet and reliance on chemoautotrophic symbionts at adult stage. Both shrimps are colonized by...
Article
Full-text available
Shallow-water hydrothermal vents are widespread, especially in the Mediterranean Sea, owing to the active volcanism of the area. Apart free microbial communities’ investigations, few biological studies have been leaded yet. Investigations of microbial communities associated with Nematoda, an ecologically important group in sediments, can help to im...
Article
Full-text available
The symbiotic shrimp Rimicaris exoculata dominates the macrofauna inhabiting the active smokers of the deep-sea mid Atlantic ridge vent fields. We investigated the nature of the host mechanisms controlling the vital and highly specialized ectosymbiotic community confined into its cephalothoracic cavity. R. exoculata belongs to the Pleocyemata, crus...
Article
Full-text available
Prokaryotes and free-living nematodes are both very abundant and co-occur in marine environments, but little is known about their possible association. Our objective was to characterize the microbiome of a neglected but ecologically important group of free-living benthic nematodes of the Oncholaimidae family. We used a multi-approach study based on...
Article
Full-text available
Deep-sea hydrothermal vent meiofauna have been the focus of recent research and the discovery of an abundant well-adapted free-living marine nematode on the Mid-Atlantic Ridge offers new perspectives on adaptations to the vent environment. Indeed, knowledge concerning biological interactions of microbes and meiofauna in marine extreme environments...
Article
Full-text available
Rimicaris exoculata is a caridean shrimp that dominates the fauna at several hydrothermal vent sites of the Mid-Atlantic Ridge. It has two distinct and stable microbial communities. One of these epibiontic bacterial communities is located in the shrimp gut and has a distribution and role that are poorly understood. The second colonizes its enlarged...
Data
The SI File is a more detailed description of bioinformatic studies followed by extraction and quantification of N-acylhomoserine lactones (AHLs) description. (DOC)
Data
LuxS protein sequences alignment. The words red/black, orange and white, are respectively used to describe the microbial mat at the end, intermediate stage, and beginning of the molt cycle. Black boxes indicate similarity of amino acids sequences. The red square shows a region necessary for the enzyme activity that is conserved in all luxS gene. (T...
Data
LuxR protein analysis. (A) LuxR protein sequence alignment. The words red/black and white are used to describe the microbial mat at the end and beginning of the molt cycle, respectively. Black boxes indicate similarity of amino acid sequences. LuxR type receptors share a modular domain structure, with a N-terminal signal binding domain (SBD) and a...
Data
Chromatograph of N-acylhomoserine lactone extraction. (A) C4-AHL standard (1) and 3-oxo-C12-HSL standard (2). (B) branchiostegite and C4-AHL extraction control (3). (C) and (F) scaphognathite. (D) abdomen and C4-AHL extraction control (4). (E) branchiostegite. (G) abdomen. (TIF)
Article
Full-text available
Prokaryotic communities forming symbiotic relationships with the vent shrimp, Rimicaris exoculata, are well studied components of hydrothermal ecosystems at the Mid-Atlantic Ridge (MAR). Despite the tight link between host and symbiont, the observed lack of spatial genetic structure seen in R. exoculata contrasts with the geographic differentiation...
Data
16S Maximum Likelihood (ML) consensus boostrap tree for Gammaproteobacteria. Tree illustrates 16 sequences obtained from Petersen et al. 2010 [14] and 100 unclassifed bacteria OTUs (denovos), all from the hydrothermal vent shrimp Rimicaris exoculata. Bootstrap support (1000 replicates, Tamura-Nei model) is located either above or below the node. Se...
Data
16S Maximum Likelihood (ML) consensus boostrap tree for Mollicutes. Tree illustrates 13 sequences obtained from Durand et al. 2015 [24] and 100 unclassifed bacteria OTUs (denovos), all from the hydrothermal vent shrimp Rimicaris exoculata. Bootstrap support (1000 replicates, Tamura-Nei model) is located either above or below the node. Sequences obt...
Data
Rimicaris exoculata sample counts by organs, molts and life stage categories. n refers to the number of total samples collected from each vent. Only adults were identified by molt color. (DOCX)
Data
Strategy for statistically testing the rejection of the null hypothesis of homogeneity of bacterial communities across molt stages, organs, life stages and vents. (DOCX)
Data
Counts and percentages of OTUs that were described only to domain level ‘bacteria’ for each sample (unclassified bacteria). Seven samples had > 25% of the OTUs described as ‘Bacteria’; all seven samples were taken from adult R. exoculata. (DOCX)
Data
16S Maximum Likelihood (ML) consensus boostrap tree for Deferribacteres. Tree illustrates 18 sequences obtained from Durand et al. 2015 [24] and 100 unclassifed bacteria OTUs (denovos), all from the hydrothermal vent shrimp Rimicaris exoculata. Bootstrap support (1000 replicates, Tamura-Nei model) is located either above or below the node. Sequence...
Data
Frequency of OTUs assigned to class for each vent, excluding unclassified bacteria. *asterisk denotes less represented phyla that include classes of Acidobacteria, Actinobacteria, Bacteroidetes, Chlorobi, Cyanobacteria, Firmicutes, GB02, SR1 and other proteobacteria. (DOCX)
Data
16S Maximum Likelihood (ML) consensus boostrap tree for Epsilonproteobacteria. Tree illustrates 19 sequences obtained from Durand et al. 2015 [24] and 100 unclassifed bacteria OTUs (denovos), all from the hydrothermal vent shrimp Rimicaris exoculata. Bootstrap support (1000 replicates, Tamura-Nei model) is located either above or below the node. Se...
Data
Geographic coordinates, depth, year(s) and number of samples sequenced for Rimicaris exoculata collections from three vents along the Mid-Atlantic Ridge (MAR) (DOCX)
Data
ANOSIM test results for dataset including unclassified bacteria. The Bray-Curtis similarity index at 9999 permutations was used (*) defines significant relationships at p = 0.05. (DOCX)
Data
ANOSIM and PERMANOVA results excluding unclassified bacteria. The Bray-Curtis similarity index at 9999 permutations was used (*) defines significant relationships at p = 0.05. (DOCX)
Data
Qualitative results of the most common haplotypes. Most common haplotypes are defined as those represented by ≥20 sequences within common operational taxonomic units (OTU) for which networks were not drawn. Each common haplotype is listed by name, number of sequences within each haplotype, and the percentage of sequences from each vent. (DOCX)
Data
Multidimensional Scaling (MDS) analyses for Rimicaris exoculata bacterial communities, including unidentified bacteria. (A) Clustering pattern by vents, with colored circles representing the vent location from where each sample was collected. (B) Clustering pattern by vent, with colored shapes and letters denoting the specific categories for each s...
Data
Frequencies of identified bacterial classes for the three life stages at Logatchev, including unidentified bacteria. Three main classes are identified across Rimicaris exoculata life stages. “Other classes” contain nine or more less common groups. (PDF)
Data
Species count and species richness for molt colors at Rainbow (A) and life stages at Logatchev (B). (PDF)
Data
Geographic network for Operational Taxonomic Unit (OTU) assigned to Gammaproteobacteria. denovo 6909 was composed of 298 sequences. Network was drawn using star-contraction before applying the median-joining calculation to illustrate the clustering of haplotypes; links are not proportional to the number of mutations and therefore, do not illustrate...
Data
ANOVA testing of OTU frequency among groups, performed using the group_significance.py script in QIIME [29]. (XLS)
Data
Alpha diversity indices, species count and species richness (Chao1 and standard error) for dataset including unclassified bacteria. (DOCX)
Data
Frequency of OTUs assigned to class for each vent, including unclassified bacteria. *asterisk denotes less represented phyla that include classes of Acidobacteria, Actinobacteria, Bacteroidetes, Chlorobi, Cyanobacteria, Firmicutes, GB02, SR1 and other proteobacteria. (DOCX)
Article
Thermophilic spore-forming bacteria are potential contaminants in several industrial sectors involving high temperatures (40–65 °C) in the manufacturing process. Among those thermophilic spore-forming bacteria, Thermoanaerobacterium thermosaccharolyticum, called “the swelling canned food spoiler”, has generated interest over the last decade in the...
Article
Full-text available
Rimicaris exoculata is a deep-sea hydrothermal vent shrimp which enlarged gill chamber houses a complex trophic epibiotic community. Its gut harbours an autochthonous and distinct microbial community. This species dominates hydrothermal ecosystems megafauna along the Mid-Atlantic Ridge, regardless of contrasted geochemical conditions prevailing in...
Article
Full-text available
The shrimp Rimicaris exoculata dominates several hydrothermal vent ecosystems of the Mid-Atlantic Ridge and is thought to be a primary consumer harbouring a chemoautotrophic bacterial community in its gill chamber. The aim of the present study was to test current hypotheses concerning the epibiont's chemoautotrophy, and the mutualistic character of...
Article
Full-text available
The caridean shrimp Rimicaris exoculata dominates the fauna at several Mid-Atlantic Ridge hydrothermal vent sites. This shrimp has an enlarged gill chamber, harboring a dense ectosymbiotic community of chemoautotrophic bacteria associated with mineral oxide deposits. Until now, their acquisition is not fully understood. At three hydrothermal vent s...
Article
Full-text available
Ventiella sulfuris Barnard and Ingram, 1990 is the most abundant amphipod species inhabiting the Eastern Pacific Rise (EPR 9°N) vent fields. This vent-endemic species is frequently encountered near colonies of Pompeii worms Alvinella pompejana. V. sulfuris specimens were collected during the oceanographic cruise LADDER II at the Bio9 (9°50.3'N, 250...
Article
Investigations of the diversity of culturable yeasts at deep-sea hydrothermal sites have suggested possible interactions with endemic fauna. Samples were collected during various oceanographic cruises at the Mid-Atlantic Ridge, South Pacific Basins and East Pacific Rise. Cultures of 32 isolates, mostly associated with animals, were collected. Phylo...
Article
Rimicaris exoculata dominates the megafauna of several Mid-Atlantic Ridge hydrothermal sites. Its gut is full of sulphides and iron-oxide particles and harbours microbial communities. Although a trophic symbiosis has been suggested, their role remains unclear. In vivo starvation experiments in pressurized vessels were performed on shrimps from Rain...