Luciano HernándezCenter for Genetic Engineering and Biotechnology | CIGB · Production Direction
Luciano Hernández
MSc
About
45
Publications
12,227
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730
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Citations since 2017
Introduction
Additional affiliations
July 1986 - present
Education
January 1993 - December 1994
August 1979 - June 1984
Facultade de Biologia de la Universidad de La Habana
Field of study
- Biology
Publications
Publications (45)
METHOD TO OBTAIN 1-KESTOSE
The present invention discloses an industrial scale method to obtain 1-kestose by the use of a plant fructosyltransferase (FTF) produced in a non-saccharolytic yeast. In this invention, the recombinant FTF is the sucrose:sucrose 1-fructosyltransferase (1-SSTrec) from Festuca arundinacea expressed constitutively and recov...
Symbiotic nitrogen fixation is critical for high soybean (Glycine max L. Merril) yields with protection to the environment. This paper describes a procedure for massive production of Bradyrhizobium japonicum in an orbital multi-platform shaker and the use of the bacterial culture for seed inoculation in soybean areas under mechanized sowing. The B....
Recombinant Streptokinase (rSK) is a protein of bacterial origin currently used in the treatment of acute myocardial infarction. Its thrombolytic and anti-inflammatory action makes it attractive for the treatment of hemorrhoids. In this work the influence of absorption enhancer, preservative and emulsifiers on rSK stability was accessed by determin...
Recombinant Streptokinase (rSK) is a protein of bacterial origin currently used in the treatment of acute myocardial infarction. Its thrombolytic and anti-infl ammatory action makes it attractive for the treatment of hemorrhoids. In this work the infl uence of absorption enhancer, preservative and emulsifi ers on rSK stability was accessed by deter...
Objective: to identify the general risks in the cleaning phase during changeover in a multiproduct plant for biological products and to put forward control actions to reduce them, guaranteeing to focus attention on the unacceptable identified risks and their scenarios. Methods: aquality risk analysis using the tool called Failure Mode and Effect An...
En este trabajo se aplicó la identificación de riesgos para la introducción de un nuevo proceso de fabricación que usa Saccharomyces cerevisiae como célula huésped en una planta multiproducto certificada, donde se fabrican productos intermedios provenientes de Escherichia coli y se proponen acciones para su control. Para ello se aplicó el análisis...
Objective: to identify the general risks in the cleaning phase during changeover in a multiproduct plant for biological products and to put forward control actions to reduce them, guaranteeing to focus attention on the unacceptable identified risks and their scenarios. Methods: aquality risk analysis using the tool called Failure Mode and Effect An...
In this study, a risk identification method is applied for introducing a new manufacturing process using Saccharomyces cerevisiae as host cell, in a certified multiproduct plant, where intermediate products are manufactured using Escherichia coli, in addition risk control actions are proposed. The quality risk analysis was applied, with the support...
The total organic carbon (TOC) test is a fast and effective analytical technique to evaluate the cleaning of biopharmaceutical manufacturing equipment. This technique can help ensure that the cleaning processes meet predetermined cleanability criteria for single and multiproduct production areas. This article presents a case study describing the us...
The molecular signals and pathways that govern biotic and abiotic stress responses in sugarcane are poorly understood. Here we describe SodERF3, a sugarcane (Saccharum officinarum L. cv Ja60-5) cDNA that encodes a 201-amino acid DNA-binding protein that acts as a transcriptional regulator of the ethylene responsive factor (ERF) superfamily. Like ot...
RESUMEN Las alteraciones de la hemostasis del organismo pueden provocar la formación de coágulos en el sistema circulatorio, que ocasionan desórdenes que llevan a procesos tales como el infarto cerebral o del miocardio. El tratamiento de estas afecciones puede requerir la aplicación de agentes fibrinolíticos como la uroquinasa (Uk), el activador ti...
A failure of hemostasis of the organism could cause the formation of clots inside the circulatory system, causing disorders of the system that lead to such processes like the stroke or myocardial infarction. The treatment of these pathologies could require the application of thrombolytic agents like the urokinase (Uk), the tissue type plasmino- gen...
A procedure for producing pineapple [Ananas comosus (L.) Merr.] transgenic plants was developed that involved selection by micropropagation in temporary immersion bioreactors (TIBs). Pineapple calluses ranging in size from 1.5 mm to 2.0 mm that were co-cultivated with Agrobacterium tumefaciens strains AT2260 (pIG121Hm) and LBA4404 (pTOK233) for 24...
The stability of freeze-dried recombinant streptokinase was studied under normal conditions of storage at temperatures between 25 and 30 °C. These temperatures represented an important advantage in the storage and transportation of the product upon not needing chain of cold. Three batches of freeze-dried product were studied in a period of 50 month...
We describe Agrobacterium tumefaciens-mediated transformation for sugarcane commercial varieties. In vitro- or field-cultured meristematic tissue and/or calluses derived from them were used as explants. The meristematic tissues were treated with an antinecrotic solution to increase cellular viability during A. tumefaciens-plant tissue interactions....
Levansucrase (LsdA) (EC 2.4.1.10) from Gluconacetobacter diazotrophicus (formerly Acetobacter diazotrophicus) yields high levels of fructo-oligosaccharides (FOS) from sucrose. A DNA fragment encoding the precursor LsdA lacking the first 57 amino acids was fused to the pho1 signal sequence under the control of the Pichia pastoris-alcohol oxidase 1 (...
This chapter discusses strategies for fructan production in transgenic sugarcane (Saccharum spp. L.) and sweet potato (Ipomoea batata L.) plants expressing the Acetobacter diazotrophicus levansucrase. Fructans are polyfructose molecules produced by the action of fructosyltransferases on sucrose. Included in the diet, fructans function as fiber beca...
The dextranase gene from the fungus Penicillium minioluteum was expressed in Escherichia coli. The recombinant endodextranase represented 1.5% of the total soluble protein. Crude recombinant enzyme preparations displayed a product profile of dextran hydrolysis as well as heat stability properties similar to those of the naturally glycosylated enzym...
Acetobacter diazotrophicus levansucrase (LsdA) is a potential new candidate enzyme for kestose production from sucrose. Culture conditions for maximal LsdA yield were investigated. Variations in the medium pH had the most significant influence on LsdA production. The highest yield (32 mg l−1) was achieved at an initial pH of 8·0, although optimal g...
Background. Recombinant streptokinase (rSk) is a streptococcal protein cloned in E. coli. Several methods have been described for streptokinase purification: ion exchange chromatography, affinity chromatography with canine plasmin and chromatography on immobilized acylated human plasminogen. Monoclonal antibodies anti-rSk immobilized to Sepharose h...
A hybrid promoter consisting of the in tandem fusion of the Tn5 nptII and the Klebsiella pneumoniae nifH promoters was constructed to study the functionality of the nif genes transcriptional activator NifA from Bradyrhizobium japonicum in two different host bacteria. beta-galactosidase experiments in E. coli revealed that the hybrid nptII-nifH prom...
The Acetobacter diazotrophicus SRT4 gene encoding levansucrase (EC 2.4.1.10) (IsdA) was isolated from a genomic library. The nucleotide sequence of a 2.3 kb DNA fragment sufficient for complementation of a levansucrase-deficient mutant (obtained by EMS treatment) was determined. The IsdA gene (1751 bp) coded for a polypeptide of molecular mass 64.9...
The main ligand-binding determinant of the human urokinase receptor (uPAR) is located in the amino terminal domain D1, but when isolated this domain presents a 1500 fold lower affinity than the intact three-domain uPAR (D1D2D3). uPAR mutants missing either domain 2 (D1HD3) or domain 3 (D1D2) were expressed in murine LB6 cells and showed to be prope...
The biosynthesis and the surface localization of the urokinase plasminogen activator receptor (uPAR) were analysed in MDCK epithelial cells and in unpolarized fibroblasts. No differences were observed with respect to rate of synthesis, nature of precursors and time of surface appearance. uPAR was localized particularly at the focal and cell-cell co...
The receptor for urokinase plasminogen activator (uPAR) acts as an anchorage site for uPA on the cell surface where it stimulates pro-uPA activation, allows the internalization of uPA:inhibitor and other complexes and sends directly or indirectly signals into the cell that may promote migration, adhesion and growth. It is a GPI-anchored, three-doma...
Streptokinase is an extracellular protein produced by several strains of streptococci. It is able to convert blood plasminogen to plasmin. In this paper we describe the purification of recombinant streptokinase using monoclonal antibodies and a combination of two affinity matrixes: human plasminogen-Sepharose 4B and monoclonal antibody antistreptok...
Streptokinase (SK), which activates human plasminogen by promoting its conversion to plasmin, is normally obtained from beta-hemolytic streptococci. Treatment with SK is an effective therapy for improving survival and preserving left ventricular function after coronary thrombosis. We report the cloning, expression in E. coli to levels of 25% of the...
Streptokinase is an extracellular protein produced by several strains of streptococci. It functions in the species-specific conversion of plasminogen to plasmin. In this paper we describe the purification of streptokinase by affinity chromatography on human plasminogen acylated with p'-nitrophenyl p-guanidinobenzoate. The acylated and non-acylated...
In this work we describe the enzymatic degradation of soluble recombinant streptokinase (rSk) by inmovilized human plasmin(ogen) - Sepharose complexed with rSk.
Human Bowes melanoma cells were entrapped in calcium alginate beads and cultivated in spinner flasks and in a fluidized-bed reactor.
Recientemente expresamos el antigeno de superficie del virus de la hepatitis B en células CHO utilizando el sistema de amplificación de la DHFR/metotrexate.
Stable hybridoma clones secreting the IgG1 mouse monoclonal antibodies (Mabs= CB-tPA 1, CB-t-PA 2 and CB-t-PA 3 were obtained from the fusion of the P3/X63 myeloma with splenic lymphocytes of mice, immunized
During 1981 we started an intensive programme for the development of biotechnology in Cuba. The first project was related to the production of leucocyte interferon and the cloning an expression of these genes in prokaryotic and yeast cells. These products are currently obtained in large amounts and their physical and chemical characterization are p...
Projects
Project (1)
The mediafill equipment we use for automatic filling of Petri dishes with culture medium, for environmental microbiological control, according to the manufacturer's method sanitization is through cleaning in place by heat, and manually using detergent, does not specify that detergent what we want to use the sanitizers we use daily in cleanroom sanitization Grade a and Grade B.
I would like to hear views on that higienizante out the most recommended for sanitizing equipment without affecting filling me with culture medium plates.
