Luca Gasperini

Luca Gasperini
University of Minho · 3B's Research Group in Biomaterials, Biodegradables and Biomimetics

Doctor of Engineering

About

31
Publications
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1,102
Citations

Publications

Publications (31)
Chapter
Introduction The hair follicle (HF) is an exclusive adnexal structure with important cosmetic and physiological value, embodying stem cells that contribute to skin homeostasis and response to injury. Therefore, sustaining de novo hair formation in damaged skin would be a clinical breakthrough both in the management of critical wounds and in irrever...
Article
Full-text available
Extracellular matrix (ECM)-based bioinks have been steadily gaining interest in the field of bioprinting to develop biologically relevant and functional tissue constructs. Herein, we propose the use of supercritical carbon dioxide (scCO2) technology to extract the ECM components of cell-sheets that have shown promising results in creating accurate...
Article
Human tissues and organs are inherently heterogeneous, and their functionality is determined by the interplay between cell types, their secondary architecture, and gradients of signalling molecules and metabolites. To mimic the dynamics of native tissues, perfusion bioreactors and microfluidic devices are widely used, enhancing cell culture viabili...
Article
The microfluidic manipulation of hydrogels is a powerful tool to recapitulate functional biological architectures. A wide range of flow configurations and chip designs have been employed to create microfibers with increasingly complex shapes and compositions requiring individually engineered setups. Distinctly, we demonstrate how one single 3D hydr...
Article
Full-text available
Background The dermal papilla (DP) represents the major regulatory entity within the hair follicle (HF), inducing hair formation and growth through reciprocal interactions with epithelial cells. However, human DP cells rapidly lose their hair inductive ability when removed from their niche and cultured in an epithelium-deficient environment. Metho...
Article
Full-text available
Engineering complex tissues requires the use of advanced biofabrication techniques that allow the replication of the tissue 3D‐microenvironment, architecture and cellular interactions. In the case of skin, the most successful strategies to introduce the complexity of hair follicle appendages have highlighted the importance of facilitating direct in...
Article
Inducing thermal gradients in two injected fluid systems results in the temporal formation of mixing conductive streams. If preserved through sol-gel transition, this mechanism can be used to drive and pat- tern non-living and living entities in mixed hydrogels. Interfaces are vital in nature, where gradients of non-living and living entities build...
Article
High-throughput strategies for optimizing biomaterials to direct cellular behaviour are a fundamental need for propelling tissue engineering and regenerative medicine. In 2D, biomaterial’s gradients have proven to be powerful platforms for simultaneously screening several surface conditions. However, their translation to 3D is yet limited to 1) exp...
Chapter
Microfluidics techniques can be used to process a wide range of biomaterials, from synthetic to natural origin ones. This chapter describes microfluidic processing of biomaterials, mainly polymeric materials of natural origin, focusing on water-soluble polymers that form non-flowing phases after crosslinking. Some polysaccharides and proteins, incl...
Article
The past 20 years have witnessed ever-growing evidence that the mechanical properties of biological tissues, from nanoscale to macroscale dimensions, are fundamental for cellular behaviour and consequent tissue functionality. This knowledge, combined with previously known biochemical cues, has greatly advanced the field of biomaterial development,...
Poster
Bioprinting, as a tissue engineering tool, enables the deposition of various cells and matrices at high resolution, accuracy and speed. These features represent a major advantage to recreate the different microenvironments/microfunctionalities found in each tissue which enlightens the potential of these biofabrication method for the development of...
Poster
RESULTS AND DISCUSSION Gellan Gum (GG), although commonly used in tissue engineering approaches due to its good biocompatibility and stability under physiological conditions, is biologically inert. No natural or man-made material can recapitulate all the features of natural extracellular matrix. Moreover, the ECM of each tissue has its very own com...
Poster
Within the field of Tissue Engineering, there is a great need for platforms that allow high-throughput (HT) screening cell responses to wide ranges of biomaterial formulations (libraries). These HT platforms can be further developed through properly manipulated gradients allowing the combination of multiple conditions that change gradually within a...
Presentation
Introduction Platforms that allow high-throughput (HT) screening of cell responses to wide ranges of biomaterial modifications are a primal challenge within Tissue Engineering. To tackle this issue, we used a natural polymer to fabricate 3D hydrogel fibers built in a gradient fashion where cells can be encapsulated and sense their varying 3-dimens...
Poster
Abstract: Skeletal muscles are prone to regenerate upon small injuries but high damages may cause myopathy with irreversible loss of muscle mass and function. Strategies involving the use of biomaterial templates loaded with functional and stimuli-responsive muscle-derived cells have been followed to prompt myogenesis. Conductive materials are appe...
Conference Paper
Platforms that allow high-throughput (HT) screening of cell responses to wide ranges of biomaterial formulations (libraries) are a primal challenge within the field of Tissue Engineering. To tackle this issue, we used a microfluidic platform to fabricate 3D hydrogel fibers built in a gradient fashion where encapsulated cells can sense a spatial inc...
Article
Design of tissue engineering strategies deals with the need to balance both biomaterials characteristics and techniques specificities, often resulting in cell-compromising processing conditions. One important factor often disregarded is the osmotic pressure to which cells are exposed to. An in-house microfluidic system was used to prove that additi...
Article
New derivatives of gellan gum (GG) were prepared by covalent attachment of octadecylamine (C18-NH2) to polysaccharide backbone via amide linkage by using bis(4-nitrophenyl) carbonate (4-NPBC) as a coupling agent. The effect of the alkyl chain grafted onto hydrophilic backbone of high molecular weight GG was investigated in terms of physicochemical...
Article
Fiber-based techniques hold great potential towards the development of structures that mimic the architecture of fibrous tissues, such as tendon. Microfluidics and polyelectrolyte complexation are among the most widely used techniques for the fabrication of fibrous structures. In this work, we combined both techniques to generate hydrogel fibers wi...
Article
Microfibers of a hyaluronic acid amphiphilic derivative (HA-EDA-C18), with incorporated dexamethasone (Dex) as a model bioactive molecule, were obtained by microfluidic technique. Exploiting the ionic strength sensible behavior of HA-EDA-C18, microfibers were formed in baths containing phosphate buffer saline with different salt concentration. The...
Conference Paper
Tendons are responsible for force transmission between muscles and bones, assuring body movement. Given the poor healing capacity of tendons, tissue engineering strategies have been focused on translating tendon architecture and functionality into biomimetic materials. In the present study, multicomponent hydrogel fibers were fabricated by combinin...
Article
Statement of significance: Methacrylated gellan gum (GG-MA) is here suggested for the first time as a widely available polysaccharide to easily prepare hydrogels with cell adhesion properties and capability of inducing the autonomous osteogenic differentiation of human adipose-derived stem cells (hASCs). GG-MA was processed as stand-alone hydrogel...
Conference Paper
Full-text available
Tendons are connective tissues whose main function is force transmission between muscles and bones, allowing body movement. Thus, tendon injuries lead to substantial morbidity, pain and disability, affecting not only athletes, but also active working people and elder population. Tissue engineering efforts have been focused on translating tendon arc...
Article
Musculoskeletal diseases are one of the leading causes of disability worldwide. Tendon injuries are responsible for substantial morbidity, pain and disability. Tissue engineering strategies aim at translating tendon structure into biomimetic materials. The main goal of the present study is to develop microengineered hydrogel fibers through the comb...
Article
In this article, we propose a systemic approach to investigate the impact of electrohydrodynamic jetting (EHDJ) encapsulation on viability, proliferation, and functionality of the encapsulated cells. EHDJ consists in applying a high-voltage electrical field between a target substrate and a jetting needle, which is fed with a suspension of cells in...
Article
SHSY5Y human neuroblastoma cells have been encapsulated in alginate microbeads by electro hydro-dynamic jetting (EHDJ) printing. The method consists in applying a high voltage electrical field between a target substrate and a jetting needle, which is fed with a suspension of cells in a pre-polymeric solution. Depending on the conditions (i.e., volt...
Article
Full-text available
The encapsulation of living mammalian cells within a semi-permeable hydrogel matrix is an attractive procedure for many biomedical and biotechnological applications, such as xenotransplantation, maintenance of stem cell phenotype and bioprinting of three-dimensional scaffolds for tissue engineering and regenerative medicine. In this review, we focu...
Article
In this work we present a bioprinting technique that exploits the electrohydrodynamic process to obtain a jet of liquid alginate beads containing cells. A printer is used to microfabricate hydrogels block by block following a bottom up approach. Alginate beads constitute the building blocks of the microfabricated structures. The beads are placed at...
Article
The encapsulation of living cells within a semi-permeable matrix is an attractive process for transplanting nonautologous cells by limiting the interaction with the host immune system. The electrohydrodynamic process is a low-cost and high-throughput system to encapsulate cells by means of a static potential. We evaluated the use of this system for...

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Projects

Projects (2)
Project
Throughout this project, we will employ microfluidics towards the fabrication of novel 3D cell-biomaterial structures to derive platforms that allow high-throughput screening cell responses towards the identification and sorting of best-fit conditions for desired outcomes. We will focus on the combination of different biomaterial properties, from mechanics to biofunctionality, and also cell-cell interactions. Envisioning multi-purpose TE applications, we will assess different responses, ranging from stem cell differentiation to the in vitro engineering of vascular systems, cancer-stromal models, among others.