Lourdes Delgado-Aceves

The objective of this work was to assess the suitability of the Droplet-vitrification protocol previously developed with Agave peacockii shoot tips for the cryopreservation of six Agave species. Shoot tips were precultured for 1 day on a medium with 0.3 M sucrose in the dark, loaded in a solution with 1.6 M glycerol and 0.4 M sucrose for 20 min, an...

El Agave maximiliana Baker es probablemente uno de los magueyes más importantes, cultural y económicamente hablando, del estado de Jalisco. A pesar de competir contra el tequila y el mezcal, la raicilla se ha abierto paso dentro del mercado internacional, demostrando el potencial de esta bebida con tanta tradición, que por mucho tiempo fue vista co...

El objetivo de este trabajo es presentar los avances logrados en la aplicación de la criopreservación a diferentes formas de cultivo in vitro de diversas especies de Agave. El método de vitrificación en criolámina resultó ser práctico y eficaz para aplicarlo a los embriones somáticos de A. tequilana cultivar ‘Chato’. El mayor rebrote posterior a la...

The husk tomato (Physalis spp.) is an exceptional commercial crop for its nutritional and medicinal properties where the whole plant is used. This has led to the search for new micropropagation methods to accelerate plant production in the field. P. angulata and P. chenopodifolia were micropropagated via shoot proliferation of axillary buds and ind...

More than 50% out of 129 of Agave species are endemic to Mexico. Among them, Agave peacockii is among the list of threatened species that require special protection. In this work, we aimed at developing new supplementary strategies to achieve micropropagation and perform cryopreservation of in vitro grown shoot-tips of A. peacockii. For multiplicat...

More than 50% of Agave species are endemic to Mexico. Among them, Agave peacockii is listed within the list of threatened species that require special protection. In this work, we aimed at developing new supplementary strategies to achieve micropropagation and perform cryopreservation of in vitro -grown shoot-tips of A. peacockii . For multiplicati...

Agave tequilana Weber cultivar ‘Chato’ represents an important genetic supply of wild severely in decline populations of ‘Chato’ for breeding and transformation programs. In this work, the indirect somatic embryogenesis and cryopreservation of Somatic Embryos (SEs) were investigated using the ‘Chato’ cultivar as a study case. Methods: Embryogenic c...

Physalis angulata is considered an important crop in Mexico, established for commercial production due to its nutritional and medicinal properties. This study’s objective was to develop an efficient method for the mass multiplication of P. angulata and cryopreservation of apical meristems by droplet-vitrification. In vitro propagation was given in...

Micropropagation systems have been developed in Agave species, however regeneration systems are frequently confused. In this study, cellular structures from organogenesis and somatic embryogenesis are discussed with several protocols in literature, as reference for future works on Agave. Axillary shoots, thin cell layer, and leaves as explants of A...

Agave tequilana cv. chato is an important resource widely used to produce a very emblematic and popular Mexican beverage called mezcal; however, its wild populations are currently severely degraded due to unsustainable management, mainly overexploitation. The application of tissue culture and cryopreservation would contribute to the propagation and...

El propóleo es un material resinoso producido por las abejas a partir de recursos naturales vegetativos; tiene propiedades antimicrobianas, antifúngicas, antivirales y antiparasitarias, las cuales varían en función de su fuente natural geográfica. El objetivo de este trabajo fue la caracterización fisicoquímica de propóleo y de su extracto etanólic...

Propolis is a resinous material produced by bees from vegetative natural sources. It has antimicrobial, antiviral and antiparasitic properties, which show variations according to its natural original source and geographic location. The aim of this work was the physicochemical characterization of propolis and its ethanolic extract [EPP] of Apis mell...

Resumen El propóleo es un material resinoso producido por las abejas a partir de recursos naturales vegetativos; tiene propiedades antimicrobianas, antifúngicas, antivirales y antiparasitarias, las cuales varían en función de su fuente natural geográfica. El objetivo de este trabajo fue la caracterización fisicoquímica de propóleo y de su extracto...

El propóleo fue colectado en el periodo de otoño mediante trampas colocadas en las colmenas. El extracto se obtuvo desgrasando el propóleo con solventes en soxhlet; extrayéndolo con etanol y concentrándolo en rotavapor a presión reducida, finalmente se liofilizo y reservó para determinación de fenoles totales, flavonoides y análisis espectroscópico...