Louis Bujan

Louis Bujan
Centre Hospitalier Universitaire de Toulouse | CHU Toulouse · CECOS (Centre d’Etude et de Conservation du Sperme) an

MD, PhD

About

214
Publications
15,387
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Introduction
Skills and Expertise
Additional affiliations
January 2003 - present
Paul Sabatier University - Toulouse III
Position
  • Research Director

Publications

Publications (214)
Article
Full-text available
We investigated the effects of dengue virus (DENV) on semen using samples collected 7, 15, 30, 60, and 90 days after symptom onset from 10 infected volunteers on Réunion Island. We assessed characteristics of semen and reproductive hormones and isolated motile spermatozoa from semen. We assayed semen for DENV using reverse transcription PCR and sea...
Article
Résumé La loi relative à la bioéthique du 2 août 2021 donne la possibilité à toute personne majeure de pouvoir conserver ses gamètes, en absence d’indication médicale, pour une utilisation ultérieure à son profit. Cette disposition soulève de nombreuses questions pratiques. Le prélèvement de gamètes étant bien plus simple chez l’homme et l’intérêt...
Article
Full-text available
Background Testicular germ cell tumours (TGCT) are the most frequent cancers in young men in developed countries and their incidence rate has doubled worldwide over the past 40 years. Early life exposures to pesticides are suspected to increase TGCT risk. Our research aimed at estimating adult TGCT risk associated with parental domestic use of pest...
Article
Study question What is the evolution of the sperm epigenome after chemotherapy in a patient with testicular cancer (TC)? Summary answer These new data on epigenetic recovery profil after TC are useful tools for counseling and reassuring these patients. What is known already An important issue for young men affected TC is how TC and its treatment...
Article
Background Many studies reported that reproductive desire could be high among transgender (TG) individuals. In France, fertility preservation (FP) and sperm donation were very little proposed to TG individuals until recently, mainly because the Bioethics Law allows the use of Assisted Reproductive Technologies (ART) only in infertile couples and pr...
Article
Full-text available
Background In the French West-Indies, few studies have been performed on fertility and sexual problems in cancer survivors, which are frequent and recurring issues reported by surveys on unmet needs. Additionally, mutualizing human and material resources and promoting cooperation through a collaborative platform are the most appropriate response to...
Article
We developed a new mutationally well-balanced 32 Y-STR multiplex (CombYplex) together with a machine learning (ML) program PredYMaLe to assess the impact of STR mutability on haplogourp prediction, while respecting forensic community criteria (high DC/HD). We designed CombYplex around two sub-panels M1 and M2 characterized by average and high-mutat...
Article
Full-text available
Background: A mild increase in testicular and epididymal temperatures in men, bulls and rams (pendulous scrotum) inhibits spermatogenesis and increases the percentage of sperm with an abnormal morphology. However, the stages of spermatogenesis that are most sensitive to a mild increase in testicular temperature in men are unknown. The aim of the p...
Article
Background: Men with congenital unilateral absence of vas deferens were reported to be mainly azoospermic, with both unilateral renal absence and mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) but some have neither. Objectives: To assess whether in infertile couples the male partners with congenital unilateral absenc...
Thesis
Full-text available
Lifestyle exposures including temperature have been studied in relationship to male reproductive health. In this project, we focused on two main parameters linked to infertility after testicular exposure of temperature. We evaluated the effects of a mild testis temperature increase (+2°C) on sperm morphology and sperm aneuploidy in five healthy men...
Article
Full-text available
In mammals, including men, testicular and epididymal temperature increase impairs spermatogenesis. In the present experimental study, we investigate the effects of a mild testis temperature increase (i.e. testis temperature remains below core body temperature) on sperm aneuploidy in men. In healthy fertile volunteers (n=5) a testicular and epididym...
Article
Full-text available
In mammals, including men, testicular and epididymal temperature increase impairs spermatogenesis. In the present experimental study, we investigate the effects of a mild testis temperature increase (i.e. testis temperature remains below core body temperature) on sperm aneuploidy in men. In healthy fertile volunteers (n=5) a testicular and epididym...
Article
Background We showed that in men with a constitutional chromosomal abnormality, DNA fragmentation was significantly higher in chromosomally unbalanced spermatozoa than in spermatozoa with a normal or balanced chromosomal content. These results could be explained by a phenomenon already described in infertile men: abortive apoptosis. Objectives To...
Article
Research question: To determine whether there is a risk of localized Zika virus (ZIKV) infection in the upper genital tract, specifically the oocytes, follicular fluids and endometrium, in exposed and/or recently infected reproductive-age women. ZIKV is an Aedes mosquito-borne Flavivirus that can lead to birth defects and to developmental anomalie...
Article
Full-text available
Background Recurrent pregnancy loss (RPL) is defined as the loss of at least three pregnancies in the first trimester. Although the most common cause is embryo aneuploidy, and despite female checkup and couple karyotyping, in about 50% of cases RPL remain unexplained. Male implication has little been investigated and results are discordant. In this...
Article
Full-text available
Background Testicular cancer (TC) represents 1% of all new male cancer cases but remains the most frequent cancer in adolescents and young adults in industrialized countries. In this study, we assessed time trends in use of sperm cryopreservation by men with TC from 1990 to 2013 in France. Methods We collected data from patients diagnosed with TC...
Article
Full-text available
Zika virus (ZIKV) is a teratogenic mosquito-borne flavivirus which can be sexually transmitted from man to woman. High viral loads and prolonged viral shedding in semen suggest that ZIKV replicates within the human male genital tract, but its target organs are unknown. Using ex vivo infection of organotypic cultures, we demonstrated here that ZIKV...
Article
Diagnosis and treatment of HIV primary infection : the earlier, the better ! In France, the HIV epidemic is far from being controlled and it is estimated that more than 7000 new infections still occur each year. HIV primary infection is a critical time in terms of the risk of contagion of the partners. The risk of evolution of the infection is clea...
Article
Full-text available
Background More and more HIV-1-infected men on effective antiretroviral treatment (ART) have unprotected sex in order to procreate. The main factor influencing transmission is seminal HIV shedding. While the risk of HIV transmission is very low, it is difficult to assess in individuals. Nevertheless, it should be quantified. Results We retrospecti...
Article
High performance assays are essential for the serological diagnosis of recent and past Zika virus (ZIKV) infections but few are presently available. We used two commercially available NS1 antigen-based enzyme-linked immunoassays to study the kinetics of anti-ZIKV IgM and IgG in 15 ZIKV-infected patients for up to 180. days after clinical onset. The...
Article
Background: Evidence of human sexual transmission during Zika virus emergence is a matter of concern, particularly in procreation, but to date, kinetics of seminal shedding and the effects of infection on human reproductive function have not been described. To investigate the effects of Zika virus infection on semen and clearance of Zika virus fro...
Article
Full-text available
Objective: To study sperm aneuploidy in a population of testicular cancer (TC) patients treated with the use of either bleomycin-etoposide-cisplatin (BEP) chemotherapy or radiotherapy. Design: Multicenter prospective longitudinal study of TC patients analyzed before treatment and after 3, 6, 12, and 24 months (T3-T24). Patient(s): Fifty-four T...
Article
Full-text available
Objective: To assess sperm production and aneuploidy in Hodgkin lymphoma (HL) and non-Hodgkin lymphoma (NHL) before and after treatments. Design: Multicenter, prospective, longitudinal study of lymphoma patients analyzed before treatment and after 3, 6, 12, and 24 months. Setting: University hospitals. Patient(s): Forty-five HL and 13 NHL pa...
Article
Full-text available
In 80% of infertile men with obstructive azoospermia caused by a congenital bilateral absence of the vas deferens (CBAVD), mutations are identified in the cystic fibrosis transmembrane conductance regulator gene (CFTR). For the remaining 20%, the origin of the CBAVD is unknown. A large cohort of azoospermic men with CBAVD was retrospectively reasse...
Article
Full-text available
The emergence of human immunodeficiency virus (HIV) infection in the 1980s drastically changed the prospects of conceiving a child for the man or woman infected with the virus. Advances in treatment then made it possible to envisage pregnancy while decreasing the risk of transmission to the child when the mother was infected. For couples where one...
Article
Background: While reproductive technologies are increasingly used worldwide, epidemiologic, clinical and genetic data regarding infertile men with combined genital tract and renal abnormalities remain scarce, preventing adequate genetic counseling. Methods: In a cohort-based study, we assessed the prevalence (1995-2014) and the clinical characte...
Article
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Objectives: Semen composition is influenced by HIV-1 infection, yet the impact of semen components on HIV infection of primary target cells has only been studied in samples from HIV-uninfected donors. Design: We compared the effect of seminal plasma from chronically HIV-infected (SP+) versus uninfected donors (SP-) on HIV-1 infection of PBMCs an...
Article
Objective: To evaluate the percentage of couples achieving parenthood and the rate of separation 6 years after their first consultation for infertility. Study design: Epidemiological study in the reproductive medicine department of a French university hospital. All first consulting couples (FCC) who had their first infertility consultation in th...
Article
Full-text available
STUDY QUESTION In which cases is freezing of ejaculated sperm indicated before ICSI? SUMMARY ANSWER Sperm freezing should be performed only when out of two analyses at least one total sperm count in the ejaculate is lower than 106. WHAT IS KNOWN ALREADY Due to variations in individual sperm parameters, in cases of severe oligozoospermia there is...
Article
Full-text available
Treatment of differentiated thyroid cancer usually consists of a total thyroidectomy followed by one or several courses of radioiodine ((131)I). (131)I is known to have deleterious effects on radiation sensitive tissues and irradiation to the testes has been shown after its administration. We investigated effects of such treatment on sperm DNA in a...
Article
Meiotic segregation of mosaic males with a 45,X cell line has been little examined. In this study, we evaluated the risk of aneuploid gametes using fluorescence in situ hybridization (FISH) and DNA fragmentation in ejaculated spermatozoa of three men with sex chromosome mosaicism including a 45,X cell line. Triple- and dual-color FISH were performe...
Article
To determine the feasibility of fertility preservation in adolescent males with cancer. Large multicenter retrospective study of male patients ≤20 years from 23 centers of a national network of sperm banks over a 34-year period. Sperm banks. A total of 4,345 boys and young men aged 11 to 20 years. Age, cancer diagnosis, feasibility of sperm banking...
Article
With effective antiretroviral therapy, the risk of mother to child transmission (MTCT) is now under 1%. The 2013 French guidelines emphasize early antiretroviral lifelong antiretroviral therapy. Thus, the current trend for women living with HIV is to take antiretroviral therapy before, during and after their pregnancies. A major issue today is the...
Article
Étudier la répartition des trois monoxide d’azote synthase (NOS) : nNOS, iNOS, eNOS entre les zones périphérique (ZP) et transitionnelle (ZT) chez des patients ayant une hyperplasie bénigne prostatique (HBP).
Article
Objective To determine consequences of lymphoma treatments on sperm characteristics and sperm DNA, and to evaluate predictors of sperm recovery. Design Multicenter prospective longitudinal study of patients analyzed before treatment and after 3, 6, 12, and 24 months. Setting University hospitals. Patient(s) Seventy-five Hodgkin lymphoma and non-...
Article
Full-text available
Background The incidence of testicular germ cell tumors (TGCT), the most common cancer in men aged 15 to 45 years, has doubled over the last 30 years in developed countries. Reasons remain unclear but a role of environmental factors, especially during critical periods of development, is strongly suspected. Reliable data on environmental exposure du...
Article
The desire for children is a legitimate aspiration that should be part of multidisciplinary care for all men, women or couples living with HIV. The use of effective antiretroviral therapy has revolutionized the prevention of sexual, as well as mother-to-child HIV transmission. When the HIV plasma viral load is undetectable on long-term antiretrovir...
Article
The desire for children is a legitimate aspiration that should be part of multidisciplinary care for all men, women or couples living with HIV. The use of effective antiretroviral therapy has revolutionized the prevention of sexual, as well as mother-to-child HIV transmission. When the HIV plasma viral load is undetectable on long-term antiretrovir...
Article
Full-text available
Male patients with chronic kidney disease often exhibit the biological and clinical hallmarks of an abnormal hypothalamo-pituitary-gonadal axis. It is known that dialysis does not reverse this impaired endocrine status; however, the impact of kidney transplantation (KT) is still controversial. The aim of our study was to investigate the levels of s...
Article
With effective antiretroviral therapy, the risk of mother to child transmission (MTCT) is now under 1%. The 2013 French guidelines emphasize early antiretroviral lifelong antiretroviral therapy. Thus, the current trend for women living with HIV is to take antiretroviral therapy before, during and after their pregnancies. A major issue today is the...
Article
Full-text available
Approximately 1% of the spermatozoa found in ejaculate of healthy men are aneuploid and this rate increases in the population of subfertile and infertile men. Moreover, fertilization with these aneuploid sperm can lead to impaired embryo development. Fluorescent In Situ Hybridization (FISH) is the common cytogenetic tool used for aneuploidy screeni...
Article
It has been previously shown that men with chromosomal structural abnormality had a higher rate of sperm DNA fragmentation. We studied 11 male carriers of a chromosomal structural abnormality (seven with a balanced reciprocal translocation, three with a Robertsonian translocation, one with a pericentric inversion) to determine whether spermatozoa w...
Article
Objective: To determine the consequences of adjuvant testicular germ cell tumor treatment (TGCT) on sperm characteristics and sperm DNA, and to evaluate the predictors of sperm recovery. Design: Multicenter prospective longitudinal study of patients analyzed before treatment and after 3, 6, 12, and 24 months. Setting: University hospitals. Pa...
Article
To add new arguments concerning the origin of the sperm-head vacuoles observed under high magnification with interference contrast microscopy, we carried out in two patients with total globozoospermia confirmed using transmission electron microscopy (TEM), a detailed sperm morphometric analysis with high magnification (×6000) under Nomarski contras...
Article
Several studies have reported a secular decrease in sperm production in man. This impairment of reproductive function is not universal, as overall these works reveal regional variations in sperm characteristics and in their change over time. A very large number of environmental or lifestyle-related factors may represent a risk to reproductive funct...
Article
Objective: To evaluate rates and reasons for treatment discontinuation in couples with male factor infertility and who failed to conceive. Design: Retrospective study. Setting: Male Sterility Center, University Hospital. Patient(s): A total of 407 couples consulting for male factor infertility and who discontinued treatment without conceivin...
Article
In France the gamete donation is based on the major principles: anonymity, no payment, solidarity and this mode of procreation can be used only if a medical indication is present in recipient couples. In prerequisite and during the revision of the law of bioethics, a wide debate took place about the anonymity of gamete donation. The objectives of t...
Article
In France the gamete donation is based on the major principles: anonymity, no payment, solidarity and this mode of procreation can be used only if a medical indication is present in recipient couples. In prerequisite and during the revision of the law of bioethics, a wide debate took place about the anonymity of gamete donation. The objectives of t...
Article
Objectives: Testicular cancer is the most common cancer in young men. Several studies have reported an alteration in semen quality in non-seminoma tumors but this result has not been confirmed in all of the published data. We performed a retrospective study in a population of 1158 men with testicular cancer who banked sperm between 1999 and 2003 in...
Article
Full-text available
Plusieurs études récentes rapportent une altération de l’appareil reproducteur chez le mâle ainsi que des différences régionales suggérant un lien entre environnement et spermatogenèse. Les histoires du dibromochloropopane, du distilbène, du gossypol, démontrent l’influence d’une exposition professionnelle, médicamenteuse ou d’un mode de vie sur l’...
Article
Full-text available
To investigate the effects of a mild induced testicular and epididymal hyperthermia (+2°C) on sperm chromatin integrity in men. Experimental prospective study. University hospital. Five healthy fertile volunteers. Testicular and epididymal hyperthermia was induced by maintaining the testes at inguinal position with the support of specially designed...
Article
We describe the antiviral effect of maraviroc in a patient who had been shedding high levels of HIV-1 in seminal fluid for three years despite an undetectable blood plasma viral load. Adding maraviroc to HAART stopped the seminal shedding. We discuss the mechanisms involved and the effect on sexual transmission.
Article
Detection of HIV-1 RNA in semen is used commonly to determine the safety of semen processing procedures before assisted reproductive technology (ART). Using two panels of prepared semen samples containing HIV-1 the performances of protocols from 14 centers have been compared. No false-positive results were detected but false-negative results were f...
Article
Full-text available
Most studies assessing the outcome of assisted reproductive technologies (ARTs) have reported live birth rates in couples by taking mainly the female factor into account. However, infertility is a couple's concern, and the majority of publications do not take into consideration the true impact of male infertility on having the desired number of chi...
Article
Full-text available
PET/CT using (18)F-FDG is a well-established diagnostic examination in oncology, cardiology and neurology. The clinical significance of nontumoral testicular uptake of FDG is unknown. Functional testicular imaging may have important clinical applications in the diagnosis and prognosis of male infertility. The aim of this study was to determine the...
Article
Management of people infected by HTLV-1 in assisted reproductive technology (ART) is a rare event. Since HTLV is sexually transmitted, HTLV serodiscordant couples have to systematically use condoms. The sperm processing (centrifugation on density gradient) performed for semen of HIV-infected men was applied. Detection of HTLV-1 provirus DNA was neg...