Loreto B Feril

Loreto B Feril
Fukuoka University · Department of Anatomy

MD, PhD

About

96
Publications
17,247
Reads
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2,476
Citations
Introduction
Loreto B Feril currently works at the Department of Anatomy, Fukuoka University. Loreto does research in Biomedical Sciences, Medical Physics, Biophysics, Radiological Sciences, Human Anatomy, and basic Cancer studies. Their current project is 'Therapeutic ultrasound'.
Additional affiliations
October 2005 - present
Fukuoka University
Position
  • Professor
Education
October 2000 - September 2004
June 1991 - March 1995
Mindanao State University
Field of study
  • Medicine
June 1983 - March 1987
Mindanao State University
Field of study
  • Physics

Publications

Publications (96)
Article
Full-text available
The use of nanobubbles (NBs) for ultrasound-mediated gene therapy has recently attracted much attention. However, few studies have evaluated the effect of different NB size distribution to the efficiency of gene delivery into cells. In this study, various size of albumin stabilized sub-micron bubbles were examined in an in vitro ultrasound (1 MHz)...
Article
The biological effects of ultrasound may be classified into thermal and nonthermal mechanisms. The nonthermal effects may be further classified into cavitational and noncavitational mechanisms. DNA damage induced by ultrasound is considered to be related to nonthermal cavitations. For this aspect, many in vitro studies on DNA have been conducted fo...
Article
PurposeTo determine the effect of low-intensity ultrasound on cancer cell proliferation in vitro and tumor growth in vivo.Methods In vitro, several cancer cell lines were exposed to low-intensity ultrasound at 0.11 W/cm2 for 2 min. Of the cell lines screened, melanoma C32 is one of the cell lines that showed sensitivity to growth inhibition by ultr...
Article
High-intensity focused ultrasound (HIFU) is a noninvasive method of ablating malignant tumors. This paper will review the current clinical application of HIFU specially in the treatment of breast cancer. In addition to clinical studies, this review will also look into some basic studies that could address the technical issues related to this modali...
Article
Full-text available
In the original version of this article, Ydyrys Almabayev's, Timur Saliev's, Aygul Almabayeva's, Baimakhan Tanabayev's, Maral Yergazina's, and Zhandos Serikpayev's first and last names were transposed.
Article
The study investigated the ability of transdermal electric stimulation to prevent the formation of intra-abdominal adhesions in the combination with Seprafilm® (anti-adhesive agent). One hundred and twenty-eight (128) rabbits were subjected to a surgical procedure to simulate the adhesion processes. After the simulation, the animals were divided in...
Article
Full-text available
Recent research has revealed that nanobubbles (NBs) can be an effective tool for gene transfection in conjunction with therapeutic ultrasound (US). However, an approach to apply commercially available hand-held diagnostic US scanners for this purpose has not been evaluated as of now. In the present study, we first compared in vitro, the efficiency...
Article
Full-text available
There has been increasing interest in using nanobubbles (NBs) for ultrasound mediated drug delivery as well as for ultrasound imaging. Albumin NBs are especially attractive for its potential of becoming a versatile platform for drug carriers and molecular targeted therapy agents. However, physical characterization of NBs is generally considered to...
Article
Full-text available
Chemoresistance is one of the major obstacles in chemotherapy‐based hepatocellular carcinoma (HCC) intervention. Aluminum (Al) is an environmental pollutant that plays a vital role in carcinogenesis, tumorigenesis, and metastasis. However, the effect of Al on chemoresistance remains unknown. 5‐Fluorouracil (5‐FU) is a widely used antitumor drug. Th...
Article
Full-text available
Using 8 methods, 337 medical students were asked to examine and to self-identify the presence of the palmaris longus muscle in each of their own arm. On the other hand, during anatomy dissection, a total of 174 cadaver arms were examined for palmaris longus muscle. This study covers the school years 2015 to 2017. The result showed that 97% of the a...
Article
Targeted microbubbles has the potential to be used for ultrasound (US) therapy and diagnosis of various cancers. In the present study, US was irradiated to oral squamous cell carcinoma cells (HSC-2) in the presence of cetuximab coated albumin microbubbles (CCAM). Cell killing rate with ultrasound treatment at 0.9W/cm² and 1.0W/cm² in the presence o...
Article
At present, the main therapeutic strategy for apoptosis induction mainly relies on the administration of pharmacological apoptotic modulators. Apart from that, apoptosis can be induced by various external stimuli such as hyperthermia, ionizing radiation, and electric fields. Despite advantages, both physical and pharmacological approaches bear some...
Article
Full-text available
After failing to kick-off during its introduction almost a century ago, it was in the recent decades wherein therapeutic ultrasound was reintroduced and gained traction in the research world. Therapeutic ultrasound is considered as a rapidly emerging field; and now, we can see broad clinical applications for this technology. The use of high-intensi...
Article
Full-text available
Undoubtedly, ultrasound imaging (sonography) remains as one of the most commonly performed medical imaging procedures today. Advances in ultrasound technology have made the procedure more reliable, thus expanding its usefulness to diagnose a wider spectrum of diseases. Among these technologies that have significantly improved the agility of diagnos...
Article
Full-text available
BACKGROUND: We scrutinized the feasibility of apoptosis induction in blood cancer cells by means of low-intensity ultrasound and the proteasome inhibitor bortezomib (Velcade). MATERIAL AND METHODS: Human leukemic monocyte lymphoma U937 cells were subjected to ultrasound in the presence of bortezomib and the echo contrast agent Sonazoid. Two types o...
Conference Paper
Full-text available
Bortezomib inhibits the 26S proteasome thus disrupting various cell signaling pathways and promoting apoptosis. It is effective against a range of tumours, but the main area of clinical applications remains treatment of blood cancer. Despite proven effectiveness, bortezomib is capable of inducing different adverse effects in dose-dependent way. We...
Conference Paper
Full-text available
One of perspective methods of cancer treatment is the induction of apoptosis (‘programmed cell death’) in malignant cells. The apoptosis can be stimulated by various factors: biological, chemical and physical. We investigated cytotoxic effect of combination of pharmacological apoptosis modulator Velcade (Janssen-Cilag Pty Ltd) and hypethermia. The...
Article
Full-text available
Ultrasound imaging, or ultrasonography, is consistently one of the most commonly utilized diagnostic procedures. This is despite the introduction of many other alternative diagnostic methods. The reasons include ultrasound being non-ionizing (unlike X-rays and CT scans), non-invasive (unlike tissue biopsies), less expensive and more commonly availa...
Article
Full-text available
Ultrasonography, or imaging by ultrasound, is one of the most commonly utilized diagnostic procedures today. This is despite the introduction of many other alternative diagnostic methods. The reasons include ultrasound being non-ionizing (unlike X-rays and CT scans), non-invasive (unlike tissue biopsies), less expensive and more commonly available...
Article
Purpose To evaluate in vitro the feasibility of therapeutic high-intensity-focused ultrasound (HIFU) combined with microbubbles and titanium dioxide (TiO2). Methods Oral squamous cell carcinoma cells (HSC-2) were sonicated using a HIFU transducer with a resonant frequency of 3.5 MHz, 30 mm in diameter, and focal length of 50 mm. The ultrasound inte...
Article
Full-text available
IntroductionIt has been shown that killing of suspended cells by low-intensity ultrasound (0.08–0.11W/cm2) can be enhanced by a mild non-lethal hypotonic (146 mOsm) medium. PurposeIn this study we wished to determine whether hypotonia-induced cell swelling of suspension cells was directly related to enhancement of ultrasound-mediated cell killing,...
Article
Full-text available
To develop a non-toxic enhancer for hyperthermia-induced cell death as a potential cancer treatment, we studied the effect and mechanism of docosahexaenoic acid (DHA) on hyperthermia-induced apoptosis. Treatment with 20 μM DHA and 44°C for 10 minutes induced significant apoptosis, increased intracellular reactive oxygen species (ROS), and caspase-3...
Article
Full-text available
To determine the effect of pulsed ultrasound (US) on radiation-induced cell killing, U937 and Molt-4 cell lines were exposed to 1.0MHz US with 50% of duty factor at 0.3W/cm(2) and pulsed at 1Hz immediately after exposure to X-rays at 0, 0.5, 2.5 and 5Gy. The cells were assayed 24h after the treatments. The result showed significant enhancement of c...
Article
Apoptosis, or programmed cell death, is a mechanism of cell death, which has been exploited for the treatment of cancers over the past few years. The understanding of apoptosis pathways (intrinsic and extrinsic) has led to discovery of treatment strategies which selectively target the cancer cells and spare the normal ones. This article reviews the...
Article
Radio-genetic therapy is a combination of radiation therapy and gene therapy that may solve some of the problems associated with conventional radiotherapy. A promoter responsive to radiation was obtained from a promoter library composed of DNA fragments created by linking the TATA box signal to randomly combined binding sequences of transcription f...
Article
Recent studies have shown that ultrasound energy could be applied for targeting or controlling drug release. This new concept of therapeutic ultrasound combined with drugs has induced a great amount of interest in various medical fields. In this paper, several experimental systems are cited in which ultrasound is being utilized to evaluate new appl...
Article
Full-text available
We chose promoters responsive to sonication in LNCap cells, a prostate cancer cell line, out of a library composed of DNA fragments constructed by linking the TATA box sequence to randomly combined cis-acting elements of transcription factors activated in response to radiation in prostate cancer cells. When a plasmid containing the luciferase gene...
Article
We previously obtained an X-ray responsive promoter from 11 promoters that we constructed. In the present study, we aimed to determine the efficiency of our promoter construction method. In addition, the reactivity of the promoter to X-rays in vivo is also investigated. Promoters constructed by linking the TATA box to randomly combined binding sequ...
Article
We previously developed an artificially constructed promoter that was activated in response to X-ray irradiation in LNCap, a prostate cancer cell line. Anticancer drugs were examined to see whether some of them could stimulate the activity of the promoter. It was found that doxorubicin (Dox) treatment to LNCap transfected with a gene cassette of th...
Article
We examined the role of ultrasound standing waves, and their travelling wave counterparts, on cell viability in an in-vitro insonation apparatus. Furthermore, the effect of distinct waveforms (sine and top-hat) was also explored, together with the role of microbubble presence. Measurements of cell viability in standing wave scenarios demonstrated a...
Article
We investigated the effects of ultrasound-mediated transfection (sonotransfection) of interferon β (IFN-β) gene on melanoma (C32) both in vitro and in vivo. C32 cells were sonotransfected with IFN-β in vitro. Subcutaneous C32 tumors in mice were sonicated weekly immediately after intra-tumor injection with IFN-β genes mixed with microbubbles. Succe...
Conference Paper
The objective for this study was to compare light-scattering based turbidity measurements on commercial lipid microbubbles, with alternative, and indeed, complementary methods for quantifying microbubble population dynamics such as flow based cytometric techniques. Microbubble populations were seen to respond to ultrasound exposure and usually exhi...
Article
Purpose Although sanazole has been used as a hypoxic radiosensitizer, we recently reported on its ability to sensitize U937 cells to hyperthermia and X-irradiation under aerobic conditions, enhancing apoptotic cell death following the combined treatment. The current study was undertaken to evaluate the effect of sanazole as a sonosensitizer under p...
Article
Full-text available
The cavitational effects of ultrasound (US) exposure induce transient pores on the cell membrane (sonoporation). Sonoporation have been applied in the field of cancer therapy by promoting delivery of extracellular molecules such as drugs and genes into cytoplasm. In addition, it is known that using US together with microbubbles (MB) elevates permea...
Article
Full-text available
To investigate whether or not the combined ultrasound and antibiotic treatment is effective against chlamydial infection, a new ultrasound exposure system was designed to treat chlamydia-infected cells. First, the minimum inhibitory concentrations of antibiotics against Chlamydia trachomatis were determined. Infected cultures were treated with anti...
Article
Sonodynamic therapy (SDT) is a new modality using ultrasound (US) to activate certain chemical sensitizers for cancer therapy. In this study, the effect of US combined with a nanoparticle titanium dioxide (TiO(2)) on melanoma cell was investigated in vitro and in vivo. Melanoma cells (C32) were irradiated with US in the presence and/or absence of T...
Article
Full-text available
The encapsulation of gas bubbles has resulted in improved stability and added a binding capacity to shells for ultrasound-guided targeted delivery. However, this has also changed the physical and acoustical properties of the final formulation. In this study, we have evaluated three clinically-used contrast agents of different compositions, namely,...
Chapter
The term sonodynamic therapy (SDT) can be described as a treatment that combines an ultrasound source with a sonosensitizing agent (a drug that can be potentiated by ultrasound) to destroy cancer cells. This context is somewhat narrow however, and is based on the early presumption that the mechanism of action involved a chemical activation of agent...
Article
Purpose In this study, the effects of low-intensity pulsed ultrasound (LIU) as an adjuvant to doxorubicin (DOX) treatment was further investigated in comparison to hyperthermia as another widely used adjuvant. The effects were compared with respect to cell killing and apoptosis induction in U937 cells. Human primary liver cancer (PLC) cells were al...
Article
We previously developed artificial promoters that were activated in response to X-ray irradiation. Sonication with 1.0 MHz ultrasound that causes intracellular oxidative stress was found to activate some of these promoters though to lesser degrees. The most sensitive one among these promoters showed intensity- and duration-dependent activations by...
Article
Purpose: To develop artificial promoters that are activated in response to sonication and to determine these properties in vitro. Methods: The binding sites of four transcription factors (nuclear factor-kappa B, activating protein-1, nuclear factor-Y, and CArG element binding factor A) that are activated by oxidative stress were randomly ligated...
Article
Ultrasound-mediated gene transfection (sonotransfection) has been shown to be a promising physical method for gene therapy, especially for cancer gene therapy. The procedure being done in vitro uses several ultrasound exposure (sonication) setups. Although high transfection rates have been attained in some of these setups in vitro, replicating simi...
Article
Purpose We have previously shown that ultrasound-mediated transfection (sonotransfection) can be optimized using a concept based on the ultrasound-induced apoptosis produced in our in vitro experiments. At optimized conditions, we have shown, using five cancer cell lines, that sonotransfection is superior to other conventional nonviral methods. Int...
Article
In this first part of the review, we will focus on and discuss various aspects of low-intensity ultrasound (US), with emphasis on mild thermal effects, apoptosis induction, and sonomechanical effects. Mild thermal effects of US have been commonly applied to physical therapy. Though US has clear beneficial effects, the advantage of using US over oth...
Article
Part one of this review focused on the thermal and mechanical effects of low-intensity ultrasound (US). In this second and final part of the review, we will focus on and discuss various aspects of low-intensity US, with emphasis on the biomolecular effects, US-mediated gene transfection (sonotransfection), and US-mediated permeabilization (sonoperm...
Article
Three novel lipid-shell-type microbubbles (MBs), AS-0100, BG6356A and BG6356B, have been evaluated for their impact on ultrasound (US)-induced cell death and free radicals production. Previously studied and well-characterized US exposure conditions were employed in which human myelomonocytic lymphoma U937 cells were exposed to 1MHz pulsed US beam (...
Article
This study was undertaken to examine ultrasound (US) mechanisms and their impact on chemical and biological effects in vitro as a function of changing pulse repetition frequency (PRF) from 0.5 to 100Hz using a 1MHz-generator at low-intensities and 50% duty factor (DF). The presence of inertial cavitation was detected by electron paramagnetic resona...
Article
To clarify the detailed molecular mechanism underlying cellular responses to nonthermal low-intensity pulsed ultrasound (LIPUS), gene expression patterns and genetic networks in human lymphoma U937 cells were examined using global-scale microarrays and computational gene expression analysis tools. Six hours after LIPUS treatment (0.3W/cm(2) for 1mi...
Article
Full-text available
The present study was conducted to examine the thermal and non-thermal effects of ultrasound on apoptosis induced by anti-CD20 monoclonal antibody (rituximab). SU-DHL-4 cells, a CD20-positive cell line derived from B cell lymphomas with a BCL2 gene rearrangement, were exposed to continuous 1 MHz ultrasound for therapeutic use under an air- or CO(2)...
Article
Potential clinical use of ultrasound (US) in enhancing the effects of anticancer drugs in the treatment of cancers has been highlighted in previous reports. Increased uptake of drugs by the cancer cells due to US has been suggested as a mechanism. However, the precise mechanism of the enhancement has not yet been elucidated. Here, the combined effe...
Article
Recently, there have been numerous reports on the application of non-thermal ultrasound energy for treating various diseases in combination with drugs. Furthermore, the introduction of microbubbles and nanobubbles as carriers/enhancers of drugs has added a whole new dimension to therapeutic ultrasound. Non-thermal mechanisms for effects seen includ...
Article
The ability of the derivatives of macrosphelides (MS) core (simplified 16-membered core structure of natural MS) to induce apoptosis in human lymphoma U937 cells was investigated. Of the five compounds examined, MS core with ketones at 8 and 14 positions (MS5) showed the highest potency to induce apoptosis, while another, MS3 with one ketone, was m...
Article
Paeoniflorin (PF) isolated from peony root (Paeoniae radix) has been used as a herbal medicine in East Asia for its anti-allergic, anti-inflammatory, and immunoregulatory effects. PF is known to cause apoptosis and to be a chemical heat shock protein (HSP) inducer. With this information, the effects on the gene expression in human leukemia U937 cel...
Article
Biological effects of low-intensity ultrasound (US) focusing on US-induced programmed cell death (apoptosis), regulation of gene expression, and US-mediated gene transfection (sonotransfection) are reviewed. Studies have shown that US can induce apoptosis and that certain conditions can provide an optimal apoptosis induction. Sonotransfection of di...
Article
Potential clinical use of ultrasound in enhancing the effects of anticancer drugs in the treatment of cancers has been highlighted in previous reports. Increased uptake of drugs by the cancer cells due to ultrasound has been suggested as a mechanism. However, the precise mechanism of the enhancement has not yet been elucidated. Here, the combined e...
Article
We have previously shown that ultrasound‐mediated gene transfection (or sonotransfection) can be optimized on the basis of concepts drawn from previous in vitro experiments demonstrating ultrasound‐induced apoptosis. At optimized conditions, we have shown using five cancer cell lines (HeLa, U937, Meth A, T24 and PC3) that sonotransfection is super...
Article
We examined the gene expression of human leukemia Molt-4 cells treated with non-thermal low intensity pulsed ultrasound. Six hours after 0.3W/cm(2) pulsed ultrasound treatment, apoptosis (24+/-3.3%, mean+/-SD) with minimal cell lysis was observed. Of approximately 16,600 genes analyzed, BCL2-associated athanogene 3 (BAG3), DnaJ (Hsp40) homolog, sub...
Article
Ultrasound-mediated gene transfection (sonotransfection) is a promising physical method for gene therapy, especially for cancer gene therapy. To investigate the optimal sonotransfection conditions and to determine whether the optimal transfection rate using sonotransfection is comparable to that of electrotransfection or liposome-mediated transfect...
Article
To verify the effect of echo-contrast agent (ECA) on apoptosis induced by ultrasound, leukemia cell lines (Jurkat, Molt-4 and U937) were sonicated at intensities previously shown to induce optimal apoptosis with or without Levovist, an ECA. The results showed that loss of viability and apoptosis can be induced in all three cell lines, apoptosis hig...
Article
Although enhancement of ultrasound-induced cell killing by photodynamic reagents has been shown, the sonochemical mechanism in detail is still not clear. Here, comparison between sonodynamic effect and photodynamic effect with photosensitizers at a concentration of 10 microM on free radical formation and cell killing was made. When electron paramag...
Article
The present study was undertaken to elucidate the mechanism by which ultrasound induces the expression of heme oxygenase-1 (HO-1). When human lymphoma U937 cells were exposed to a 1 MHz continuous wave for 1 min, HO-1 expression examined by real-time quantitative polymerase chain reaction and immunoblotting was observed at intensities above the cav...
Article
To determine whether nitric oxide (NO) can be released from a cage compound N,N'-bis(carboxymethyl)-N,N'-dinitroso-p-phenylenediamine sodium salt (BNN 5 Na), we sonicated different concentrations of BNN 5 Na solutions containing an NO spin trap, (MGD)2Fe2+, and then measured (MGD)2Fe2+-NO signal using electron paramagnetic resonance (EPR). We also...
Article
Previously, we have investigated different factors that enhance or inhibit the bioeffects of low intensity ultrasound particularly those involving the non‐thermal mechanisms. Several enhancing factors were identified just as there were factors that can inhibit the bioeffects. Although in some instances where free radicals were detected, free radica...
Article
Purpose The present study was undertaken to reconfirm heme oxygenase-1 (HO-1) induction by ultrasound, and elucidate the mechanism by which this occurs. Methods After exposure of human lymphoma U937 cells to 1 MHz continuous ultrasound (US), gene profiling by using cDNA microarray analysis, cell viability by using the trypan blue dye exclusion test...