Lorenzo León

Lorenzo León
Instituto de Investigación y Formación Agraria y Pesquera | IFAPA · Departamento de Mejora Genética y Biotecnología

PhD Agronomy

About

152
Publications
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2,860
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Citations since 2016
52 Research Items
1930 Citations
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2016201720182019202020212022050100150200250300
2016201720182019202020212022050100150200250300
Introduction
I work on olive breeding aiming at obtaining new cultivars with early bearing, high productivity, resistance to different biotic factors (Verticillium, Xylella) and abiotic factors (temperature, water stress), adaptation to different growing systems, high oil content and quality. Three new cultivars have been released in collaboration IFAPA/UCO (‘Sikitita’ series: ‘Sikitita’, ‘Sikitita2’, ‘Sikitita 3’) and two new selection resistant to Verticillium wilt are currently under registration.

Publications

Publications (152)
Article
Full-text available
Olea europaea subsp. cuspidata has a relatively low commercial value due to the low size and pulp to stone ratio of its drupes compared to commercial olive cultivars. Nevertheless, this subspecies could represent a valid source of useful traits for olive breeding. In the current work, the drupe metabolic composition (secoiridoids, flavonoids, simpl...
Article
Full-text available
Despite the importance of flowering for fruit formation, it has been considered very little in breeding programs involving fruit species, including olives. We evaluated the principal morphological flower-quality components in the olive cultivars, ‘Arbequina’ and ‘Picual’, and in the progenies of their crosses. Wide ranges of variation were obtained...
Article
Full-text available
The long juvenile period in olive (Olea europaea L.) delays the evaluation of characters of interest and prolongs the selection of new cultivars in the breeding programs. Therefore, it is important to use accurate selection criteria and appropriate selection pressure to make an effective identification of the superior genotypes and to identify whic...
Article
Full-text available
Hedgerow orchard is an olive growing system where trees are planted at a super high-density higher than 20-fold (i.e., 1200–2500 trees ha ⁻¹ ) compared to the traditional density of olive orchards (usually 50 to 160 trees ha ⁻¹ ). It is dominating a great proportion of new plantations because harvesting can be fully mechanized, it is early bearing...
Article
Some adaptation measures in response to the severe impacts of climate change on Mediterranean olive orchards were evaluated under a wide range of weather conditions. For this task, a decision support system for improving resources management by the integration of the AdaptaOlive simulation model, perturbed climate and impact and adaptation response...
Article
In the present work, we compared the phenol content and composition of fruit from the ‘Arbequina’ cultivar in four Mediterranean (in Andalucía, Southern Iberian Peninsula) and two Sub-Tropical (Canary Islands) locations throughout the harvest period. Two Mediterranean and two Sub-Tropical locations were maintained with drip irrigation, while the re...
Conference Paper
Full-text available
Since 2015, a large-scale olive screening program for identifying sources of resistance to Xylella fastidiosa subsp. pauca ST53 (Xfp) was started under natural (field) and controlled conditions (greenhouse, GH) in Apulia (southern Italy). Approx. 100 genotypes including olive cultivars (from 15 different olive-growing countries) and breeding select...
Article
The purpose of this study was to evaluate the relationship between two types of vigor and architectural parameters (quantitative and qualitative) and the duration of juvenile period in olive and to test the effectiveness of three different strategies for an early elimination of seedlings with long juvenile period. Architectural parameters were eval...
Article
Full-text available
Background Olive orchards are threatened by a wide range of pathogens. Of these, Verticillium dahliae has been in the spotlight for its high incidence, the difficulty to control it and the few cultivars that has increased tolerance to the pathogen. Disease resistance not only depends on detection of pathogen invasion and induction of responses by t...
Conference Paper
Olive is a woody crop extended over 10 Mha around the world (FAOSTAT, 2019), being Spain the country with the largest area (2.7 Mha). Andalusia is located in the South of Spain, with 1.6 Mha cultivated with olive trees, most of them (around 90%) dedicated to olive oil production (MAPA, 2020). This region is characterized by a great diversity of wea...
Article
Full-text available
Harvest timing in olive orchards has a strong effect on the quality and quantity of oil yield, but many farmers still lack simple and affordable quantitative tools for rationally deciding appropriate harvest dates. This study presents and tests a conceptual model for predicting fruit oil content (Of, g oil fruit⁻¹) from inexpensive measurements of...
Article
Full-text available
Genetic resistance is the most recommended measure to control verticillium wilt in olive (VWO), a vascular disease caused by the soil-borne fungus Verticillium dahliae, which has promoted in recent years the development of olive breeding programs aiming at obtaining new resistant and highly yielding cultivars. Screening has been commonly performed...
Article
Olive flowering phenology is highly affected by climatic conditions. Phenological models have been developed to forecast flowering date on olive mainly based on temperature. These models have used flowering datasets collected from trees growing under Mediterranean climatic conditions. In most of the cases, in those conditions, chilling requirements...
Article
Several factors affect the volatile fraction of extra virgin olive oil (EVOO). In this work, three advanced breeding selections and “Picual” cultivar are evaluated in comparative fields under different climatic conditions, to test and quantify the effect of genetic (G) and environmental (E) factors and their interaction (GxE). Significant differenc...
Article
Olive oil is highly valued by its nutritious and healthy function mainly due to its chemical composition. Oil chemical components are highly influenced by the cultivar and the environment, resulting in some cases in levels out of the limits set by the International Olive Council. In the present research, we used a multi-environment trial with five...
Article
Extra virgin olive oil (EVOO) chemical composition is characterized by high content of monounsaturated fatty acids and minor compounds including phenols, sterols, tocopherols, squalene and volatile compounds. These components are related to EVOO quality in terms of healthy properties, shelf life alteration due to susceptibility to oxidative degener...
Article
Full-text available
Background: The species Olea europaea includes cultivated varieties (subsp. europaea var. europaea), wild plants (subsp. europaea var. sylvestris), and five other subspecies spread over almost all continents. Single nucleotide polymorphisms in the expressed sequence tag able to underline intra-species differentiation are not yet identified, beyond...
Article
Analyzing the molecular variability of genes potentially related to disease resistance in olive (Olea europaea L.) could be useful to develop marked assisted strategies for breeding as well as for general management of genetic resources. This work studied nucleotide diversity of 77 different genotypes from cultivated and wild subsp. europaea, subsp...
Conference Paper
Full-text available
Cultivated olive (Olea europaea subsp. europaea var. europaea) is one of the most ancient cultivated fruit trees with a huge genetic heritage. More than 1200 olive cultivars are conserved in olive germplasm collections around the world. A core collection of 36 accessions was defined in the largest world olive germplasm bank of IFAPA based on geneti...
Article
Full-text available
Wild subspecies of Olea europaea constitute a source of genetic variability with huge potential for olive breeding to face global changes in Mediterranean-climate regions. We intend to identify wild olive genotypes with optimal adaptability to different environmental conditions to serve as a source of rootstocks and resistance genes for olive breed...
Article
The control of the olive tree vigor is a critical factor to ensure adequate production and longevity of high-density olive cultivation. To deal with this problem, we intend to take advantage of the genetic variability present in the wild subspecies of Olea europaea to identify genotypes of reduced vigor with optimal adaptability to diverse soils an...
Article
Full-text available
Aim of study: Crop phenology is a critical component in the identification of impacts of climate change. Then, the assessment of germplasm collections provides relevant information for cultivar selection and breeding related to phenology, being the base for identifying adaptation strategies to climate change. Area of study: The World Olive Germpla...
Conference Paper
Olive crop constitutes the main economic and social crop in numerous areas of the Mediterranean basin. However, recently there is a growing concern about the future sustainability of this crop due to the limited profit and the increasing frequency of climatic hazards as droughts and heat events. The tool AdaptaOlive integrates different simplified...
Article
Full-text available
Olive is a long-living perennial species with a wide geographical distribution, showing a large genetic and phenotypic variation in its growing area. There is an urgent need to uncover how olive phenotypic traits and plasticity can change regardless of the genetic background. A two-year study was conducted, based on the analysis of fruit and oil tr...
Article
Full-text available
Flowering is one of the most critical processes in the reproductive phenology in fruit trees including olive. Under Mediterranean climate, olive flowering period normally occurs from early to late spring. Therefore, flowering time could be affected by environmental conditions such as water availability and temperature, thus influencing flower quali...
Article
Olive (Olea europaea L.) is one of the most important fruit crops in the Mediterranean Basin, because it occupies significant acreage in these countries and often has important cultural heritage and landscape value. This crop can be infected by several Meloidogyne species (M. javanica, M. arenaria, and M. incognita, among others), and only a few cu...
Article
Full-text available
Virgin olive oil (VOO) is the main source of lipids in the Mediterranean diet and one of the main contributors to its proven protection against diseases associated with chronic inflammation states. This oil is rich in antioxidant compounds such as tocopherols, which together constitute the vitamin E stock of the oil. The purpose of the present work...
Article
Full-text available
The pattern of olive oil accumulation is of paramount importance in olive because its influence in determining final oil yield and optimal harvesting date. In this work, we have evaluated the genetic and environment influence on the olive oil accumulation pattern. For that purpose, a set of cultivars and breeding selections planted in a multi-envir...
Conference Paper
Olive is an evergreen tree species that is cultivated in 10.8 million hectares all over the world, being more than 2.5 million hectares planted in Spain (FAO, 2017). Andalusia, in Southern Spain, is the main region of the world both by surface and production. This region is characterized by a great diversity of soil types and weather conditions. Fo...
Poster
Full-text available
In recent years a change in the traditional cropping model under rain-fed regime is being transformed into intensive and superintensive olive groves undergoing fertilization and irrigation. This means the use of low vigor cultivars of early entry into production and higher branching habit like Arbequina, currently the most used cultivar in super hi...
Article
AdaptaOlive is a simplified physically-based model that has been developed to assess the behavior of olive under future climate conditions in Andalusia, southern Spain. The integration of different approaches based on experimental data from previous studies, combined with weather data from 11 climate models, is aimed at overcoming the high degree o...
Article
Ethanol is a substrate for the chemical synthesis of fatty acid ethyl esters (FAEE) during storage of virgin olive oil whose contents are officially regulated. Given the impact that the ethanol content might have on the olive oil commercialization, the level of this metabolite has been studied in an array of olive genotypes representing the diversi...
Article
Full-text available
A wide genetic diversity has been reported for wild olives, which could be particularly interesting for the introgression of some agronomic traits and resistance to biotic and abiotic stresses in breeding programs. However, the introgression of some beneficial wild traits may be paralleled by negative effects on some other important agronomic and q...
Article
Full-text available
Olive growing is mainly based on traditional varieties selected by the growers across the centuries. The few attempts so far reported to obtain new varieties by systematic breeding have been mainly focused on improving the olive adaptation to different growing systems, the productivity and the oil content. However, the improvement of oil quality ha...
Article
In olive, both discontinuous canopy (open vase configuration) and continuous canopy (hedgerow) systems are used in commercial planting. Reliable and cost effective plant architecture characterization is necessary to test the suitability of cultivars to specific growing systems, particularly in olive breeding programs where a large number of genotyp...
Article
Due to the severe increase projected in future temperatures and the great economic and social importance of olive growing for vast agricultural areas in the Mediterranean Basin, accurate climate change impact assessment on olive orchards is required. The aim of this study is to assess the flowering date and the impact of mean and extreme temperatur...
Article
Full-text available
Fatty acids are the main components of the olive oil and their composition has a critical influence on the oil quality. However, oil quality evaluation has not been frequently included in the selection of new bred cultivars. This can be due to the difficulties in analyzing oil quality in large set of genotypes and also to the long juvenile period o...
Article
Full-text available
The need for new improved cultivars adapted to modern cultivation techniques and changing climatic environment requires genetic studies able to provide a better understanding of the phenotypic variance decomposition into genetic and environmental variances. In perennial crops, this decomposition requires evaluations carried out among years, trees w...
Article
Squalene, phytosterols and tocopherols are minor constituents of paramount importance for the olive fruit and oil quality. The objective of this research was to conduct a two-year evaluation of these compounds in the fruits of seven advanced breeding selections. They were mainly selected for early bearing and high oil content from progenies of cros...
Article
Olive oil is highly valued for its nutritional quality mainly determined by its fatty acid composition together with the presence of several biologically active minor components. In olive breeding programs, these oil quality components are normally evaluated in the later stages of selection because oil extraction is not suitable for being performed...
Article
In the present study, olive fruits from six cultivars grown under similar agronomical and environmental conditions were collected at four different times during fruit ripening. Some agronomical traits were determined and a general increase in the size of the fruit and oil contents were recorded for all cultivars. Phenolic compounds in fruits were a...
Article
This study aimed to identify new sources of resistance to Verticillium wilt in olive. We evaluated various types of genotypes: wild olive trees (Olea europaea subsp. europaea var. sylvestris), genotypes belonging to related subspecie (Olea europaea subsp. guanchica) and genotypes coming from crosses between Picual cultivar and wild olive trees. Fif...
Article
Volatile compounds are responsible for most of the sensory qualities of virgin olive oil and they are synthesized when enzymes and substrates come together as olive fruit is crushed during the industrial process to obtain the oil. Here we have studied the variability among the major volatile compounds in virgin olive oil prepared from the progeny o...
Article
Full-text available
Climatic changes impact fruit tree growth and severely limit their production. Investigating the tree ability to cope with environmental variations is thus necessary to adapt breeding and management strategies in order to ensure sustainable production. In this study, we assessed the genetic parameters and genotype by environment interaction (GxE) d...
Article
Verticillium wilt (VW) resistance was evaluated in genotypes from olive crosses including resistant cultivars as parents. Thirty-eight genotypes from three crosses were evaluated: ‘Changlot Real’ × ‘Dolce Agogia’ (16), ‘Frantoio’ × ‘Arbosana’ (13) and ‘Koroneiki’ × ‘Empeltre’ (9). These genotypes were previously selected for several agronomic trait...
Article
Full-text available
The development of reliable methods for the estimation of crown architecture parameters is a key issue for the quantitative evaluation of tree crop adaptation to environment conditions and/or growing system. In the present work, we developed and tested the performance of a method based on low-cost unmanned aerial vehicle (UAV) imagery for the estim...
Article
Verticillium wilt, a vascular disease caused by the soilborne fungus Verticillium dahliae Kleb., constitutes currently the major cultivation constraint in many olive growing areas. An integrated disease management strategy is recommended for Verticillium wilt control including the use of resistant cultivars as one of the most efficient control meas...
Article
Full-text available
The evaluation of the relative susceptibility of new cultivars to the main diseases of a crop is a key point to consider prior to their release to the nursery industry. This study provides a rigorous characterization of the resistance of 15 new olive cultivars and their genitors (‘Arbequina’, ‘Frantoio’, and ‘Picual’) to the three main aerial disea...
Article
Leaves from six important olive cultivars grown under the same agronomic conditions were collected at four different times from June to December and analyzed by high performance liquid chromatography-diode array detector-time of flight-mass spectrometry (HPLC-DAD-TOF-MS). 28 phenolic compounds were identified and quantified. No qualitative differen...
Conference Paper
Full-text available
PESTOLIVE (Contribution of olive history for the management of soil-borne parasites in the Mediterranean Basin) is a project funded by ARIMNet, an ERANET action supported by the 7th European Framework Programme and by non-European Mediterranean countries. PESTOLIVE aims at producing knowledge and tools for a new and efficient management of plant-pa...
Article
Full-text available
Wild olives (Olea europaea subsp. europaea var. sylvestris) may offer new and interesting plant material which can be used either as rootstocks or genitors in breeding programs. Recent studies, carried out at in situ and ex situ level, and focused on morpho-agronomic and genetic (by means of SSRs, DArTs and cpDNA markers) diversity, have shown that...
Article
The use of resistant cultivars is considered one of the most important ways to control Verticillium wilt of olive in the framework of an integrated disease management strategy. However, only a reduced number of cultivars currently in use have shown a considerable level of resistance to the development of symptoms. Wild olives may represent a useful...
Article
In the last years, olive cultivation is evolving in the main olive producing countries from traditional to more intensive growing systems. Increasing productivity, shortening the unproductive period and adopting tree structure suitable for mechanical harvesting are some of the main objectives of these new systems. In this intensification process, h...
Article
‘Sikitita’ is a new olive cultivar developed in Spain by crossing the cultivars ‘Picual’ and ‘Arbequina’. The phenolic fraction of olive leaves (Olea europaea L.), is purported to have good anti-oxidative properties which help to prevent several health problems. To our knowledge, no studies are available on the phenolic fraction of ‘Sikitita’ olive...
Article
Until recently, olive breeding programmes have been based exclusively on cultivated olive germplasm (Olea europaea subsp. europaea var. europaea), in contrast to breeding in other fruit crops where the use of wild relatives has been widely reported. In this study, ten agronomic traits were evaluated in two progenies derived from controlled crosses...
Article
Selection for oil quality is commonly conducted at the latest stages of olive breeding programs, as oil quality traits are measured in extracted oils. At the initial stages of breeding, the number of genotypes is high and fruit production is low, which makes it difficult to conduct oil extraction. The objective of this research was to evaluate the...
Article
Development of methodologies for early selection is one of the most important goals of olive breeding programs at present. In this context, the identification of molecular markers associated with beneficial alleles could allow the development of marker-assisted selection (MAS) strategies in olive breeding programs. Fruit-related and plant vigor tra...
Article
Several olive breeding programs have been initiated in the last years to obtain new olive cultivars adapted to modern olive growing techniques. However, up to now there is limited information about the inheritance of the main agronomic traits in olive (Olea europaea L.). In the present work, twenty six morphological descriptors and agronomic traits...
Article
Full-text available
Virgin olive oil phenolic compounds are responsible for its nutritional and sensory quality. The synthesis of phenolic compounds occurs when enzymes and substrates meet as olive fruit is crushed during the industrial process to obtain the oil. The genetic variability of the major phenolic compounds of virgin olive oil was studied in a progeny of th...
Article
Information on olive breeding is very scarce and particularly concerning selection strategies to obtain new olive cultivars, while for other fruit tree crops different possibilities of resources allocation have been reported regarding the number of selection steps, number of replications and number of characters under evaluation and phenotyping met...