Longshan Zhao

Shenyang Pharmaceutical University | SYPHU · Department of Pharmacy

A novel pH-responsive magnetic graphene oxide composite (MGO@PEI-BA) is proposed for the first time as an adsorbent for the rapid capture and detection of nucleosides (cytidine, uridine, guanosine, and adenosine). The morphology, structure, and magnetic properties of the composite were evaluated using various characterization techniques. The result...

A vortex-assisted dispersive liquid–liquid microextraction (VA-DLLME) procedure using hydrophobic deep eutectic solvent–based ferrofluid (HDES-FF) as an extractant was established. The developed sample preparation method coupled with high-performance liquid chromatography–diode array detector (HPLC–DAD) was applied to the pretreatment and determina...

The continuous accumulation of shrimp shell waste in daily life is not only a source of environmental pollution but also a waste of resources. The conversion of shrimp shell waste into valuable bioactive components using cost-effective and environmentally friendly methods is still of research interest. Based on the above intention, in this study, a...

Gushudan (GSD) has the effect of strengthening bones and nourishing kidneys. However, its specific intervention mechanism still remains unclear. In this study, to investigate the pathogenesis of glucocorticoid-induced osteoporosis (GIOP) and the preventive mechanism of GSD on GIOP, fecal metabolomics based on 1 H-NMR and ultra-high-performance liqu...

Enrofloxacin (ENR) residue is harmful to humans and the ecosystem. Therefore, it is necessary to develop a method for the detection of ENR residues in food. Herein, we have prepared novel biomass-derived carbon dots (BCDs) by a one-step hydrothermal method to detect ENR. The precursor material is cicada slough, which is one of the traditional crude...

Kidney-yang-deficiency-syndrome is a neuroendocrine disease caused by the dysfunction of adrenal-pituitary-target gland axis. Gushudan is a traditional Chinese medicine prescription with functions of tonifying kidney and strengthening bone, and its bone-strengthening effect has been confirmed by previous anti-osteoporosis research. However, its kid...

Ethnopharmacological relevance: Dyslipidemia is the leading risk factor of atherosclerosis (AS). Adipose tissue macrophages (ATMs) can regulate postprandial cholesterol levels via uptake and hydrolyzation of lipids and regulation of macrophage cholesterol efflux (MCE). San-wei-tan-xiang (SWTX) capsule, a Traditional Chinese medicine, exerts clinic...

A strategy to enhance the peroxidase-like activity of the hemin composite is presented. The Cu-Salt-Fe with enhanced activity was synthesized by one-step heat treatment and applied to the colorimetric determination of free cholesterol in human serum. Phosphate can act in complexing Cu²⁺ to form a carrier Cu-Floc with a large specific surface area....

Ceramides are important intermediates in the metabolism of sphingolipids. High-throughput liquid chromatography-mass spectrometry has been used extensively for monitoring the levels of serological ceramides, but is still limited by inadequate coverage or lack of sensitivity. Herein, a rapid, sensitive, and high-throughput isotope dilution liquid ch...

For the first time, a PEC immunosensor based on a signal amplification strategy is successfully constructed to quantitatively detect alpha-fetoprotein in serum sample. Three favorable factors explain the ultra-high sensitivity of this method. Firstly, compared with pure BiPO4, the BiPO4/BiOBr heterojunction has a narrower band gap, which expands th...

A green, fast, and efficient pH-induced natural deep eutectic solvent combined with vortex-assisted dispersive liquid-liquid microextraction method (pH-NADES-VA-DLLME) followed by HPLC was established for determination of ofloxacin (OFL), ciprofloxacin (CIP) and enrofloxacin (ENR) in honey. In this method, NaOH, as an emulsifier, can increase the c...

Pitaya seed carbon dots (P-CDs) with good biocompatibility were synthesized by hydrothermal method using natural biological matrix as carbon source, which applied to the detection of humic acid (HA) for the first time. So far, there is no report on the use of biomatrix-derived carbon dots for the detection of HA. This method can bring less pollutio...

Yaobitong capsule is a compound preparation of traditional Chinese medicine which has been widely applied in disease treatment. To insight into the therapeutic effects of yaobitong capsule on rheumatoid arthritis and its mechanisms, a LC‐MS untargeted urine metabolomics method was established and validated, combined with the quantitative analysis o...

Surfactants are one of the major pollutants in laundry powder, which have an impact on the environment and human health. Carbon quantum dots (CQDs) are spherical zero-dimensional fluorescent nanoparticles with great potential for fluorescent probing, electrochemical biosensing and ion sensing. Herein, a bottom-up approach was developed for the synt...

In this study, a magnetic dispersive micro-solid phase extraction (MD-μ-SPE) pretreatment method assisted by deep eutectic solvent-magnetic molecularly imprinted polymers (DES-MMIPs) was established in combination with UHPLC-UV analysis method for extraction and determination of Hydrocortisone (HC), cortisone (COR) and dexamethasone (DEX) in lotion...

In order to meet the demand for the separation of complex samples, the development of mixed-mode chromatographic stationary phases has become one of the research priorities in chromatographic separation. In this work, carbon dots, Ser-OCDs, with amino, hydroxyl, carboxyl and alkyl chains were synthesized for the first time and modified on silica ge...

In this paper, an environmentally friendly and facile method for the synthesis of CuCo2O4 nanosheets using deep eutectic solvent was reported. The synthesized CuCo2O4 nanosheets were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), zeta potential research, Brunauer-Emmett-Teller (...

An efficient analysis platform composed of nanozyme-based hydrogel kit and smartphone was constructed for on-site detection of uric acid (UA) in a rapid and realiable manner. CuCo2S4 nanoparticles (CuCo2S4 NPs) as a peroxidase mimic were successfully prepared and the peroxidase-like activity and catalytic mechanism were studied in detail. The hydro...

Impurity element doping is widely considered to be one of most efficient methods to adjust the electronics construction and the photocatalyst photo-generated separation of charge. In this work, a series of novel flower-like La-doped MnIn2S4 photocatalysts (x wt.% La-doped MnIn2S4, x=0, 5, 10, 20, 30) were prepared by hydrothermal synthesis and adop...

Dopamine (DBA) as an important biomarker, plays a crucial role in disease diagnosis. In this study, we have developed a fast and simple aptamer-based fluorescence strategy which used single-wall carbon nanohorns (SWCNHs) as a quencher for dopamine detection. SWCNHs were negatively charged after pretreated, which improved its dispersion in solution....

The pharmacodynamics, ¹H NMR metabolomics and endogenous network pharmacology strategy approaches were integrated to investigate the preventive mechanism of Gushudan (GSD) on kidney-yang-deficiency-syndrome (KYDS) rats in this study. Firstly, the KYDS rat model was achieved by hydrocortisone induction, and the efficacy of GSD on KYDS model rats was...

It plays an important role to effective detection of amphenicols antibiotic residues in food and an important issue considering of possible impact on human health. In this work, the molecularly imprinted membranes (MIMs) were proposed to simultaneously recognize and detect thiamphenicol (TAP), florfenicol (FF) and chloramphenicol (CAP) in pork and...

In this paper, we developed a novel nanocomposite sensor toward detection of glutathione (GSH) and glutathione S-transferase (GST) via convenient colorimetric analysis. The ZnCo2O4 sheets were synthesized by a deep eutectic solvent, and decorated with Au nanoparticles (AuNPs) as well as bovine serum albumin (BSA) by a simple chemical reduction tech...

In this work, blue-emitting chicken cartilage carbon dots (cc-CDs) were synthesized using biomass as green precursors. The abundant heteroatoms in biomass allowed for self-doping during the synthesis of cc-CDs. The experimental results showed that cc-CDs possessed excellent optical properties and good stability. The elemental composition, graphitiz...

Yaobitong capsule (YBTC), a Chinese medicine compound preparation, has been demonstrated to affect multiple pathways associated with inflammation and exhibit potential anti-arthritis effect. In this study, an integrated omic approach based on UHPLC-Q-TOF MS and 16S rRNA sequencing analyses was proposed to reveal the anti-arthritis effect and possib...

In this study, a novel dithiocarbamate functionalized magnetic chitosan graphene oxide (DTC-Chm-GO) nanocomposite was synthesized for the first time and used for simultaneously dyes and heavy metal ions adsorption as well as bacterial disinfection in complex wastewater. The maximum adsorption capacities of DTC-Chm-GO for the four pollutants calcula...

An ionic liquid-based dispersive liquid–liquid microextraction (IL-DLLME) combined with magnetic solid-phase extraction (MSPE) was developed for extraction of quinolones (quinolones) from honey and milk prior to high-performance liquid chromatography (HPLC) analysis. 1-Butyl-3-methylimidazolium hexafluorophosphate was used as the extraction solvent...

A magnetic molecularly imprinted polymer based on deep eutectic solvent was synthesized for specific identification and quantification of vanillin. Fe3O4@SiO2-C=C and deep eutectic solvent were applied as the carrier of magnetic material and functional monomer, respectively. According to composition and morphology characterizations and adsorption k...

Lysozyme (Lyz) is well known as a kind of cancer marker, and the detection of Lyz is essential for early diagnosis cancers and further effective treatment. Herein, this work firstly proposed a synthesized strategy of silica cross-linked molecularly imprinted polymers and we applied it for construction of fluorescent biosensor determining Lyz. At pH...

Yaobitong capsule is a valuable traditional Chinese medicine prescription (TCMP), which can effectively treat lumbar disc herniation clinically. However, the effective substances in Yaobitong capsule are still unclear due to a lack of metabolic studies. This poses a huge obstacle preventing the clinical safety assessment and quality control of Yaob...

As a kind of abundant resource, the development of biomass has been drawn quite more curiosity to the present research community. In this study, a facile, environment friendly, and repeatable one-step hydrothermal synthesis approach of CDs (carbon dots) by using carp roe as raw material was established. The CDs prepared from carp roe did not requir...

Yaobitong capsule (YBTC) has been used for the prevention and treatment of inflammation-related lumbago and leg pain. However, its intervention mechanism still remains unclear. This study was aimed to evaluate the control efficiency of YBTC on adjuvant-induced rheumatoid arthritis (RA) rats by metabonomic method and to explore its possible anti-art...

A novel dual-emission ratiometric fluorescent probe based on N-doped yellow fluorescent carbon dots (y-CDs) and blue fluorescent copper nanoclusters (CuNCs) was established for quantitative determination of Cu2+ and biothiols. In this work, the Cu2+-(y-CDs) complexes formed by the chelation of y-CDs with Cu2+, showed an absorption peak at 430 nm th...

A new type of magnetic chitosan nanocomposites modified with graphene oxide and polyethyleneimine (MCS/GO-PEI) was synthesized, which was used as an adsorbent to remove the toxic heavy metals of As and Hg as well as anionic azo dyes of congo red and amaranth in environmental water. In this experiment, MCS/GO-PEI was first synthesized and the struct...

As a crucial biothiol, glutathione (GSH) plays a key role in the organisms. Monitoring GSH level is of great significance for disease diagnosis and biomedical research. In this work, polydopamine (PDA) nanoparticles-red fluorescent carbonized polymer dots (r-CPDs) based ratiometric fluorescence sensing platform was constructed and employed for GSH...

An excellent magnetic multi-walled carbon nanotubes (MMWCNT) containing carboxyl material modified with ferroferric oxide (Fe3O4) nanoparticles was synthesized as the adsorbent for magnetic solid-phase extraction (MSPE) of five heavy metal ions (Pb2+, Cu2+, Co2+, Cd2+, Cr4+) in water samples followed by on-line inductively coupled plasma mass spect...

A novel benzimidazole-derived active Schiff base fluorescence probe 1-[[(1H-1,3-benzodiazol-2-yl)imino]methyl]naphthalen-2-ol (HBN) was designed, synthesized and characterized with high selectivity toward Cu²⁺ based on “OFF-ON-OFF” aggregation-induced emission (AIE) fluorescence strategy. HBN exhibited strong fluorescence enhancement in water. It w...

Acetylcholinesterase (AChE) plays a crucial role in regulating the levels of the neurotransmitters in organisms. In this study, a dual-optical signals ratiometric sensing system based on fluorescence and scattered light was constructed by using silicon quantum dots (SiQDs) and MnO2 nanosheets for AChE activity assay. The two independent signals of...

A target-dependent ratiometric fluorescence sensing strategy was designed and fabricated based on a redox reaction for highly sensitive detection of α-glucosidase (α-Glu) activity and its inhibitor. In this study, silicon quantum dots (SiQDs) with excellent optical properties and two-dimensional (2D) cobalt oxyhydroxide (CoOOH) nanosheets were succ...

A simple, quick, effective turn-on fluorescence assay for the determination of fipronil (FIP) was built based on the yellow fluorescence of FAM-aptamer and excellent quenching capability of the oxidized single-walled carbon nanohorns (The oxidized SWCNHs). Oxidized SWCNHs with the great advantage of good dispersibility in solution were generated by...

In this work, novel dummy molecularly imprinted membranes (MIMs) were fabricated using the nylon-66 (NY-66) membranes as the subtracts based on an eco-friendly “sandwich” technology with less consumption of organic reagents at mild conditions for recognition and extraction of enrofloxacin (ENR) and ciprofloxacin (CIP) in egg samples. The prepared M...

A novel green pH-sensitive fluorescent probe based on the surface passivation was designed for sensitive, rapid and reliable detection of pirimicarb (PRC). Using natural deep eutectic solvent (NADES) as the green precursor, N-CDs were hydrothermally synthesized, and combined with rhodamine B (RhB) to display strong fluorescence emission peaks at 40...

A novel dispersive magnetic solid phase microextraction (d-MSPE) method using ionic liquid-coated amino silanized magnetic graphene oxide ([email protected]2-APTES-IL) as adsorbent has been established for enriching and extracting lead(II), copper(II) and cadmium(II) in shellfish samples. The novel nanocomposite was proved synthesized successfully...

Myelodysplastic syndrome (MDS) is neoplastic disease originating from hematopoietic stem cells, and hematopoietic stem cell transplantation (HSCT) is currently the most effective cure despite lenalidomide, azacytidine and decitabine were applied to relieve symptoms of MDS. The purpose of this study is to evaluate the changes of endogenous metabolit...

The escaped β-agonists and dyes during conventional wastewater treatment processes pose a non-negligible threat to humans and environment. In this study, a novel method based on the thiol modified chitosan magnetic graphene oxide nanocomposite (Fe3O4@SiO2/GO/CS/MPTS) was reported for the effective removal of β-agonists and dyes as well as the disin...

随着绿色化学的发展,开发和应用符合绿色化学要求的溶剂和方法备受关注。作为离子液体类似物,低共熔溶剂(deep eutectic solvent, DES)是通过氢键受体(hydrogen bond acceptor, HBA)和氢键供体(hydrogen bond donator, HBD)的氢键作用而形成的一种混合物,具有环境友好、制备简单、成本低、可生物降解等优点,在很多领域均有越来越广泛的应用。DES可以从不同样品中萃取和分离不同的目标化合物,其作为萃取溶剂具有独特的优势,可以获得较高的萃取效率且样品基质对分析过程的影响较小。在分散液液微萃取(dispersive liquid-liquid micro-extraction, DLLME)程序中,DES可以萃取复杂基质中的残留药物、金...

Based on the traditional Chinese medicine theory, kidney is considered to govern the bones and dominate the store of essence (‘jing’ in Chinese). Gushudan (GSD) is a traditional Chinese medicine prescription for the treatment osteoporosis in the clinic and is beneficial for improving kidney function and strengthening bone in vivo. This study aims t...

In present study, DL-lactic acid/trioctylmethylammonium chloride-based deep eutectic solvent (DES), which was considered as an environmental friendly extraction solvent to replace traditional organic reagent, was firstly presented for monitoring of trace cadmium (Cd) and arsenic (As) in wine samples by ultrasonic-assisted dispersive liquid-liquid m...

A rapid, reliable and eco-friendly method for the determination of three sex hormones in five kinds of milk was developed and validated by combining vortex-assisted liquid-liquid microextraction (VALLME) and magnetic solid-phase extraction (MSPE). Deep eutectic solvents (DESs) such as choline chloride/urea were considered as the extraction solvent...

In this study, a novel of magnetic molecularly imprinted polymers (Fe3O4/GO/DMIPs) with multi-targets recognizing function were prepared by surface molecular imprinting technique adopting isoprenaline as the dummy-template molecule and graphene oxide (GO) as the carrier. The morphology, structures and magnetic properties of nanosorbents were charac...

Objective: To establish a method for extraction and determination of antioxidant 264 (2,6-di-tert-butyl-4-methylphenol) in the brominated butyl rubber stopper for injection and migration study in recombinant potent antitumor and antivirus protein injection (Novaferon). Methods: Dichloromethane-ethanol was adopted as the extraction solvent during...

Herein, we report a novel sensor to detect trypsin using a purpose-designed Fluorescein-labelled peptide with negatively charged carbon nanoparticles (CNPs) modified by acid oxidation. The fluorescence of the Fluorescein-labelled peptide was quenched by CNPs. The sensor reacted with trypsin to cleave the peptide, resulting in the release of the dye...

A novel of magnetic dummy-template molecularly imprinted polymers (mag-MWCNTs-DMIPs) were prepared by surface molecular imprinting technique. The structure of polymers were characterized and the binding properties were assessed by adsorption experiments. The synthetic mag-MWCNTs-DMIPs exhibit satisfying adsorption capacity, excellent selectivity an...

Ethnopharmacological relevance Yaobitong capsule (YBTC) was a traditional Chinese medicine (TCM) and it had clinically used to treat lumbar disc degeneration (LDH) for a long time. However, the active ingredients of YBTC absorption into the plasma and its pharmacological mechanism of treatment for LDH still remained unclear. Aim of the study In th...

Nitrogen and sulfur co-doped carbon dots (N, S-CQDs) with high fluorescent, water-soluble, low-toxicity properties were synthesized by microwave-assisted hydrothermal approach. The prepared N, S-CDs exhibited high selectivity in detection of tetracyclines (TCs) and displayed a fast-responsive fluorescence quenching signal in the mixture, which are...

A simple rapid and efficient deep eutectic solvent-based magnetic colloidal gel (DES-MCG) assisted magnetic solid-phase extraction (MSPE) method followed by high performance liquid chromatography with a diode array detector (HPLC-DAD) was established for determination of four sex hormones (including ethinylestradiol, norgestrel, megestrol acetate a...

A novel thiol (provided by (3-mercaptopropyl) trimethoxysilane, MPTS) modified chitosan magnetic graphene oxide nanocomposite (Fe3O4@SiO2/GO/CS/ MPTS) was firstly synthesized and characterized as an efficient magnetic sorbent for enrichment and extraction of trace level of clenbuterol in pork samples (muscle, fat, heart and liver). Various greatly...

A reliable and sensitive ultra performance liquid chromatography tandem mass spectrometry (UPLC‐MS/MS) method was firstly established and validated for simultaneously determine seven active ingredients of Yaobitong capsule in rat plasma, including ginsenoside Rg1, ginsenoside Rb1, osthole, tetrahydropalmatine, paeoniflorin, albiflorin and ferulic a...

This work described the development of a novel method for simultaneous extraction of eight active compounds (including catechin, albiflorin, paeoniflorin, ferulic acid, ginsenoside Rg1, tetrahydropalmatine, ginsenoside Rb1 and osthole) from Yaobitong capsule by accelerated solvent extraction (ASE). Response surface methodology (RSM) with desirabili...

In this work, a method for the determination of the amounts of the four genotoxic impurities in gefitinib has been developed. A simple and sensitive high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) approach has been developed, optimized, and validated. The genotoxic impurities were 3-chloro-4-fluoroaniline, 3,4-difluoroa...

A green and highly efficient ultrasound-assisted deep eutectic solvent extraction combined with functionalized magnetic multi-walled carbon nanotubes solid-phase extraction method for determination of seven pesticide residues in food products was developed. Various types of deep eutectic solvents (DESs) were screened for high extraction efficiencie...

An integrated metabolomic strategy based on both RP-UHPLC-Q-Orbitrap HRMS and HILIC-UHPLC-Q-Orbitrap HRMS in rat plasma was developed and validated, to further understand the anti-osteoporosis effect of Gushudan (GSD) and its mechanism on glucocorticoid-induced osteoporosis (GIOP) rats in this study. The metabolites were separated and identified on...

Introduction: The Epimedium herb, Yinyanghuo in Chinese, is a famous Chinese herbal medicine. In this study, an efficient extraction method was developed for the extraction of major bioactive constituent epimedin A, epimedin B, epimedin C and icariin from E. pubescens Maxim. using deep eutectic solvents (DESs). Methodology: A series of choline c...

A simple and efficient method involving microwave assisted extraction (MAE) combined with GC‐MS was established to determine 1,3‐Di‐tert‐butylbenzene (DBB), 2,4‐Di‐tert‐butylphenol (DBP), 3,5‐Di‐tert‐butyl‐4‐hydroxybenzaldehyde (DBHBA), for the purpose of evaluating the compatibility with drug packaging materials. In this study, the antimicrobial p...

A novel multi-residue method was developed for the simultaneous analysis of tetracyclines and β-receptor agonists in chicken by coupling pressurized liquid extraction (PLE) with ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The parameters of PLE method was optimised, such as extraction solvents, extraction tem...

A sensitive, fast, eco-friendly ultrasound assisted liquid-liquid microextraction (LLME) in combination with hydrophobic deep eutectic solvent (DES) method was developed to preconcentrate and extract sulfonamides in fruit juices, prior to high-performance liquid chromatographic (HPLC) analysis. The DES was synthesized with trioctylmethylammonium ch...

This manuscript presents an effective and robust method for simultaneous stereoselective determination of two pyrethroid insecticides-tetramethrin and α-cypermethrin in different food products by HPLC. Enantioseparation was carried out using reversed-phase chromatography and the influences of four polysaccharide-based chiral column, mobile phase co...

A novel polyaniline-polypyrrole functionalized SiO2-coated magnetic graphene oxide composite (MGO/SiO2@PANI-PPy) was prepared as a new magnetic solid-phase extraction (MSPE) adsorbent for separation and pre-concentration of chromium (Cr) and lead (Pb) at trace level in food and environmental water samples followed by using inductively coupled plasm...

This work presented a novel method for the simultaneous enantioselective analysis of six chiral pesticides in seven functional foods at enantiomeric levels. Samples were extracted from matrices using a magnetic solid-phase extraction method based on Fe3O4 modified carbon nanospheres as adsorbent. Experimental variables were optimized using one-vari...

The silica-coated magnetic graphene oxide (Fe3O4[email protected]2) composites were synthesized by a simple procedure in this work. The nanocomposite was characterized and applied for the rapid magnetic solid-phase extraction (MSPE) of chromium (Cr), cobalt (Co), nickel (Ni), copper (Cu), cadmium (Cd), lead (Pb), silver (Ag) ions at trace level in...

Palbociclib (named PD 0332991) is a novel highly selective cyclin-dependent kinase 4 and 6 (CDK 4/6) inhibitor, which has been approved by the Food and Drug Administration (FDA) for the treatment of hormone-receptor-positive advanced breast cancer. This present study developed a comprehensive strategy to investigate the metabolic profile of palboci...

Due to the misuse of hormones and antibiotics resulting in drug residues in meat products, serious concerns have arisen regarding to the potential risks for human health. In this paper, a simple and efficient method was developed to simultaneously quantify six steroid hormones and six antibiotics in fatty foods by combining pressurized liquid extra...

A kind of green solvents termed deep eutectic solvent (DES) have been considered alternatives to traditional organic solvents. In this study, a method was developed for determination of five pesticide residues including triadimenol, fipronil, tebuconazole, hexaconazole and diniconazole in five different traditional Chinese medicines (TCMs). In the...

1. Mosapride is a potent gastroprokinetic agent, and des-p-fluorobenzyl mosapride (M1) and mosapride-N-oxide (M2) are its two major active metabolites. 2. The validated ultra high performance liquid chromatography-tandem mass spectrometry method was successfully applied to the distribution and excretion of mosapride and its two active metabolites....