Loic Briand

Loic Briand
Center for Taste, Smell and Feeding Behaviour

PhD

About

228
Publications
61,325
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3,329
Citations
Additional affiliations
September 2007 - present
French National Institute for Agriculture, Food, and Environment (INRAE)
Position
  • Research Director

Publications

Publications (228)
Article
G protein-coupled olfactory receptors (ORs) enable us to detect innumerous odorants. They are also ectopically expressed in non-olfactory tissues and emerging as attractive drug targets. ORs can be promiscuous or highly specific, which is part of a larger mechanism for odor discrimination. Here, we demonstrate that the OR extracellular loop 2 (ECL2...
Article
Full-text available
In the gut ecosystem, microorganisms regulate group behaviour and interplay with the host via a molecular system called quorum sensing (QS). The QS molecule 3-oxo-C12:2-HSL, first identified in human gut microbiota, exerts anti-inflammatory effects and could play a role in inflammatory bowel diseases where dysbiosis has been described. Our aim was...
Article
Introduction Artificial sweeteners (AS) are well known to activate two categories of receptors; sweet taste receptor with high affinity and bitter taste receptors with low affinity. Interestingly, recent data from our and other groups suggested that these two receptors families are expressed throughout the arterial tree. Therefore, it could be inte...
Article
Full-text available
Plasmalogens are a specific glycerophospholipid subtype characterized by a vinyl-ether bound at their sn- 1 moiety. Their biosynthesis is initiated in the peroxisome by dihydroxyacetone phosphate-acyltransferase (DHAPAT), which is encoded by the DAPAT gene. Previous studies have shown that plasmalogen-deficient mice exhibit major physiological dysf...
Preprint
Full-text available
In the gut ecosystem, microorganisms regulate group behaviour and interplay with the host via a molecular system called quorum sensing (QS). The QS molecule 3-oxo-C12:2-HSL, first identified in human gut microbiota, exerts anti-inflammatory effects and could play a role in inflammatory bowel diseases where dysbiosis has been described. Our aim was...
Article
Full-text available
The worldwide demand for pulse-based products is increasing in the face of climate change, but their acceptability is limited due to the presence of off-flavours. Off-notes contribute to negative perceptions of pulses (beany notes). Volatile compounds belong to a large variety of chemical classes. They are mainly produced from the oxidation of unsa...
Article
Full-text available
Sweet taste perception is mediated by a heterodimeric receptor formed by the assembly of the TAS1R2 and TAS1R3 subunits. TAS1R2 and TAS1R3 are class C G-protein-coupled receptors whose members share a common topology, including a large extracellular N-terminal domain (NTD) linked to a seven transmembrane domain (TMD) by a cysteine-rich domain. TAS1...
Article
Full-text available
Four oleanane-type glycosides were isolated from a horticultural cultivar “Green Elf” of the endemic Pittosporum tenuifolium (Pittosporaceae) from New Zealand: three acylated barringtogenol C glycosides from the leaves, with two previously undescribed 3-O-β-d-glucopyranosyl-(1→2)-[α-l-arabinopyranosyl-(1→3)]-β-d-glucuronopyranosyl-21-O-angeloyl-28-...
Preprint
G protein-coupled olfactory receptors (ORs) enable us to detect innumerous odorants. They are also ectopically expressed, emerging as attractive drug targets. ORs can be promiscuous or highly specific, which is part of Nature′s strategy for odor discrimination. This work demonstrates that the extracellular loop 2 (ECL2) plays critical roles in OR p...
Conference Paper
Full-text available
Flavour perception results from the interactions of many molecules in mouth, including the ones that occur between oral enzymes and odorants or tastants, which are released upon food mastication. Taste perception results from the activation of taste receptors present in the taste buds, which are bathed in saliva. As a result, molecules eliciting th...
Preprint
Full-text available
The transduction of odorant binding into cellular signaling by olfactory receptors (ORs) is not understood and knowing its mechanism would enable developing new pharmacology and biohybrid electronic detectors of volatile organic com-pounds bearing high sensitivity and selectivity. The electrical characterization of ORs in bulk experiments is subjec...
Preprint
The transduction of odorant binding into cellular signaling by olfactory receptors (ORs) is not understood and knowing its mechanism would enable developing new pharmacology and biohybrid electronic detectors of volatile organic com-pounds bearing high sensitivity and selectivity. The electrical characterization of ORs in bulk experiments is subjec...
Article
Full-text available
The scarcity of transcriptional regulatory genes in Buchnera aphidicola, an obligate endosymbiont in aphids, suggests the stability of expressed gene patterns and metabolic pathways. This observation argues in favor of the hypothesis that this endosymbiont bacteria might contribute little to the host adaptation when aphid hosts are facing challengi...
Article
Introduction Originally thought to be physiologically inert, artificial sweeteners are more and more recognized to alter various biological functions through their interaction with the sweet taste receptor in extraoral sites. Alarmingly, recent epidemiological data have shown that frequent consumption of artificial sweeteners (AS) is associated wit...
Poster
The B vitamins are a class of water-soluble vitamins that play an important role in maintaining normal physiologic and metabolic functions. Among these eight B vitamins, only five (B1, B2, B3, B5 and B6) exhibit a bitterness mainly responsible for the off-taste of nutritional supplements [1]. This negative sensation can lower the product acceptabil...
Article
Full-text available
The survival of insects depends on their ability to detect molecules present in their environment. Odorant-binding proteins (OBPs) form a family of proteins involved in chemoreception. While OBPs were initially found in olfactory appendages, recently these proteins were discovered in other chemosensory and non-chemosensory organs. OBPs can bind, so...
Article
Full-text available
Odorant metabolizing enzymes (OMEs) are expressed in the olfactory epithelium (OE) where they play a significant role in the peripheral olfactory process by catalyzing the fast biotransformation of odorants leading either to their elimination or to the synthesis of new odorant stimuli. The large family of OMEs gathers different classes which intera...
Article
Flavor is one of the main drivers of food consumption and acceptability. It is associated with pleasure feels during eating. Flavor is a multimodal perception corresponding to the functional integration of information from the chemical senses: olfaction, gustation, and nasal and oral somatosensory inputs. As a result, astringency, as a sensation me...
Article
Full-text available
The excess consumption of added sugar is consistently found to be associated with weight gain, and a higher risk of type 2 diabetes mellitus, coronary heart disease, and stroke. In an effort to reduce the risk of cardiometabolic disease, sugar is frequently replaced by low- and null-calorie sweeteners (LCSs). Alarmingly, though, emerging evidence i...
Article
Full-text available
The presence of an inactivating HSP110 mutation in colorectal cancers has been correlated with an excellent prognosis and with the ability of HSP110 to favor the formation of tolerogenic (M2‐like) macrophages. These clinical and experimental results suggest a potentially powerful new strategy against colorectal cancer: the inhibition of HSP110. In...
Article
Full-text available
Smell and taste impairments are recognized as common symptoms in COVID 19 patients even in an asymptomatic phase. Indeed, depending on the country, in up to 85–90% of cases anosmia and dysgeusia are reported. We will review briefly the main mechanisms involved in the physiology of olfaction and taste focusing on receptors and transduction as well a...
Article
Full-text available
Brazzein is a small sweet-tasting protein found in the red berries of a West African evergreenshrub,Pentadiplandra brazzeanaBaillon. Brazzein is highly soluble and stable over a large pH rangeand at high temperatures, which are characteristics that suggest its use as a natural sweetener.However,Pentadiplandra brazzeanaculture is difficult at a larg...
Article
Full-text available
Orally Disintegrating Tablets (ODTs) are used to restore the nutritional status of people suffering from swallowing pathologies such as dysphagia. ODTs are consumed without water intake and are easily swallowed. The main active compounds of ODTs are vitamins and minerals. These nutrients can have a bad taste or aftertaste, which can be masked by sw...
Book
Savez-vous que nous goûtons avec le nez ? Le goût nous est familier et pourtant, il constitue l’un des sens les plus complexes. Les scientifiques ont découvert les récepteurs qui permettent à nos papilles de percevoir les différentes saveurs.Savez-vous que nous avons un seul type de récepteur à la saveur sucrée alors que nous possédons 25 récepteur...
Book
Full-text available
Article
Xenobiotic metabolizing enzymes and other proteins, including odorant-binding proteins located in the nasal epithelium and mucus, participate in a series of processes modulating the concentration of odorants in the environment of olfactory receptors and finely impact odor perception. These enzymes and transporters are thought to participate in odor...
Presentation
Full-text available
Brazzein is a small heat and pH-sable sweet-tasting protein naturally found in berries of the West African plant, Pentadiplandra brazzeana. Brazzein combines a high sweet potency, a long history of human consumption and a remarkable thermal stability. Thus, brazzein has a great potential as a natural sweetener. Previous works using site-directed en...
Article
Full-text available
Odorant-binding proteins (OBPs) are small soluble proteins that are thought to transport hydrophobic odorants across the aqueous sensillar lymph to olfactory receptors. A recent study revealed that OBP28a, one of the most abundant Drosophila OBPs, is not required for odorant transport, but acts in buffering rapid odour variation in the odorant envi...
Chapter
Vertebrate odorant-binding proteins (OBPs) are small soluble proteins abundantly secreted in the olfactory mucus of many animal species, including humans. Vertebrate OBPs reversibly bind odorant molecules with micromolar range affinities. Although their physiological role is not clearly understood, OBPs are proposed to carry airborne odorants towar...
Article
Full-text available
The sense of taste has a role in human nutrition, it help to orient our food choices and avoid the ingestion of toxi compounds. Advances in molecular biology, cell biology an, biochemistry were used to identify and characterize the main taste receptors. The identification of these receptors allow a better understanding of the physiological processe...
Article
Sugar replacement is still an active issue in the food industry. The use of structure-taste relationships remains one of the most rational strategy to expand the chemical space associated to sweet taste. A new machine learning model has been setup based on an update of the SweetenersDB and on open-source molecular features. It has been implemented...
Article
Full-text available
La saveur amère permet aux mammifères d'éviter l'ingestion d'aliments potentiellement contaminés par des substances amères toxiques fréquemment retrouvées dans notre environnement. La détection de ces molécules aux structures chimiques diverses est réalisée par une famille de récepteurs appelés TAS2R. L'être humain est pourvu de 25 TAS2R aux spectr...
Article
Full-text available
The detection and processing of chemical stimuli involve coordinated neuronal networks that process sensory information. This allows animals, such as the model species Drosophila melanogaster, to detect food sources and to choose a potential mate. In peripheral olfactory tissues, several classes of proteins are acting to modulate the detection of c...
Article
The intention to send a crewed mission to Mars involves a huge amount of planning to ensure a safe and successful mission. Providing adequate amounts of food for the crew is a major task, but 20 years of feeding astronauts on the International Space Station (ISS) have resulted in a good knowledge base. A crucial observation from the ISS is that ast...
Article
Juvenile hormones (JHs) regulate important processes in insects, such as postembryonic development and reproduction. In the hemolymph of Lepidoptera, these lipophilic sesquiterpenic hormones are transported from their site of synthesis to target tissues by high affinity carriers, the juvenile hormone binding proteins (JHBPs). Lepidopteran JHBPs bel...
Article
Full-text available
Animals need to detect in the food essential amino acids that they cannot synthesize. We found that the odorant binding protein OBP19b, which is highly expressed in Drosophila melanogaster taste sensilla, is necessary for the detection of several amino acids including the essential l-phenylalanine. The recombinant OBP19b protein was produced and ch...
Article
Full-text available
Nutritional supplements are prescribed when one’s nutritional status is not conducive to good health. These foodstuffs constitute concentrated sources of nutrients such as vitamins, minerals, amino acids, and fatty acids. For nutritional supplements to be effective, patients must consume the amount that has been prescribed for the recommended perio...
Article
Purpose of review: Polyphenols display beneficial health effects through chemopreventive actions on numerous chronic diseases including cancers, metabolic disorders, reproductive disorders and eating behaviour disorders. According to the principle of chemoreception, polyphenols bind cellular targets capable of accepting their stereochemistry, name...
Article
Full-text available
The olfactory epithelium is continuously exposed to exogenous chemicals, including odorants. During the past decade, the enzymes surrounding the olfactory receptors have been shown to make an important contribution to the process of olfaction. Mammalian xenobiotic metabolizing enzymes, such as cytochrome P450, esterases and glutathione transferases...
Article
Full-text available
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.
Article
Pro-survival stress-inducible chaperone HSP110 is the only HSP for which a mutation has been found in a cancer. Multicenter clinical studies demonstrated a direct association between HSP110 inactivating mutation presence and excellent prognosis in colorectal cancer patients. Here, we have combined crystallographic studies on human HSP110 and in sil...
Article
Full-text available
Odorant-metabolizing enzymes are critically involved in the clearance of odorant molecules from the environment of the nasal neuro-olfactory tissue to maintain the sensitivity of olfactory detection. Odorant metabolism may also generate metabolites in situ, the characterization and function of which in olfaction remain largely unknown. Here, we eng...
Article
Andrew Taylor, Jonathan Beauchamp, Loic Briand, Victor Demaria Pesce, Martina Heer, Thomas Hummel, Scott McGrane, Christian Margot, Serge Pieters, Paola Pittia and Charles Spence consider the role of food flavour in combatting under-consumption of nutrients by astronauts on the Mars expedition.
Presentation
Chemoperception is critical for animals to detect nutritive food and avoid toxic compounds. As many other insects, Drosophila melanogaster uses various semiochemicals including pheromones and food volatiles to identify a suitable ecological niche and a mating partner. Chemosensory detection is mostly mediated by olfactory and gustatory systems invo...
Article
Gurmarin is a highly specific sweet-taste suppressing protein in rodents that is isolated from the Indian plant Gymnemasylvestre. Gurmarin consists of 35 amino acid residues containing three intramolecular disulfide bridges that form a cystine knot. Here, we report the crystal structure of gurmarin at a 1.45 Å resolution and compare it with previou...
Article
Nowadays, monitoring of volatile organic compounds (VOCs) is very important in various domains. In this work, we aimed to develop sensitive olfactory biosensors using odorant binding proteins (OBPs) as sensing materials. Three rat OBP3 derivatives with customized binding properties were designed and immobilized on the same chip for the detection of...
Article
The interaction of tannins with salivary proteins is involved in astringency. This paper focussed on saliva lining oral mucosae, the mucosal pellicle. Using a cell-based model, the impact of two dietary tannins (EgC and EgCG) on the mucosal pellicle structure and properties was investigated by microscopic techniques. The role of basic Proline-Rich-...
Article
Full-text available
The data presented in this article are related to the research article entitled “Characterization of a Drosophila glutathione transferase involved in isothiocyanate detoxification.” (Gonzalez et al., 2018) [1]. This article includes the expression level of Drosophila melanogaster GSTE1 and GSTE7 in chemosensory male tissues and the expression level...
Presentation
Chemoperception plays a crucial role in the detection of nutritive food and avoidance of toxic compounds. It also allows animals to identify a suitable ecological niche and mating partners. As many other insects, Drosophila melanogaster possess a very sensitive chemosensory ability to detect and discriminate a wide panel of semiochemicals. Chemosen...
Presentation
Chemoperception plays a crucial role in the detection of nutritive food and avoidance of toxic compounds. It also allows animals to identify a suitable ecological niche and mating partners. As many other insects, Drosophila melanogaster possess a very sensitive chemosensory ability to detect and discriminate a wide panel of semiochemicals. Chemosen...
Article
Full-text available
Glutathione transferases (GSTs) are ubiquitous key enzymes that catalyse the conjugation of glutathione to xenobiotic compounds in the detoxification process. GSTs have been proposed to play a dual role in the signal termination of insect chemodetection by modifying odorant and tasting molecules and by protecting the chemosensory system. Among the...
Article
The field of chemical senses has made major progress in understanding the cellular mechanisms of olfaction and taste in the past two decades. However, the molecular understanding of odour and taste recognition is still lagging far behind and will require solving multiple structures of the relevant full-length receptors in complex with native ligand...
Article
La réduction du sucre s'accélère en 2018. Zoom sur les dernières avancées de la recherche dédiée aux récepteurs gustatifs. Les aromaticiens focalisent leurs recherches sur la mise au point d'arômes modulateurs qui équilibrent le profile aromatique des produits allégés.
Book
Brazzein is a small heat- and pH-stable sweet-tasting protein isolated from the West African plant, Pentadiplandra brazzeana. Brazzein combines a highly sweet potency, a long history of human consumption, and a remarkable stability, giving it great potential as a natural sweetener. Due to the difficulties of obtaining brazzein from its natural sour...
Article
Full-text available
Umami taste perception is mediated by the heterodimeric G-protein coupled receptors (GPCRs), formed by the assembly of T1R1 and T1R3 subunits. T1R1 and T1R3 subunits are class C GPCRs whose members share common structural homologies including a long N-terminal domain (NTD) linked to a seven transmembrane domain by a short cysteine-rich region. The...
Data
SDS-PAGE analysis of cT1R1-NTD expressed in bacteria. E. coli BL21 (DE3) cells were transformed with pET28-cT1R1-NTD before and after IPTG induction for 1, 2 and 3 hours. Soluble and insoluble protein fractions (inclusion bodies) were separated and loaded onto 12% SDS-polyacrylamide gel with size control of molecular mass markers (lane M). Proteins...
Data
Production of pure refolded cT1R1-NTD. (A) Schematic summary of key steps in the production of pure refolded cT1R1-NTD. Blue numbers on the left indicate samples analyzed by SDS-PAGE (B) and western-blot analysis using anti-his-tag antibodies (C). The proteins were separated by 4–15% SDS-PAGE and stained with Coomassie blue. The molecular mass mark...
Data
Amino acid sequence of cT1R1-NTD. cT1R1-NTD sequence (Leu21-Ser495) is in green. Numbers refer to amino acid residues of cT1R1 (Signal peptide: 1–20). His-Tags and thrombin cleavage site are shown in yellow and pink, respectively. The cysteine residue located in loop 3 expected to form a disulphide bridge between cT1R1 and cT1R3 subunits is shown i...
Data
Characterization of cT1R1-NTD folding. cT1R1-NTD intrinsic tryptophan fluorescence in the absence (solid line) and presence of 6M guanidine hydrochloride (GuCl) (dashed line). Excitation wavelength 295 nm and temperature of the cuvette was maintained at 20°C. (TIF)