Lo Gorton

Lo Gorton
Lund University | LU · Department of Chemistry

PhD, professor

About

598
Publications
78,366
Reads
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28,164
Citations
Citations since 2016
86 Research Items
7953 Citations
201620172018201920202021202202004006008001,0001,200
201620172018201920202021202202004006008001,0001,200
201620172018201920202021202202004006008001,0001,200
201620172018201920202021202202004006008001,0001,200
Additional affiliations
December 1974 - present
Lund University
Position
  • Professor (chair)

Publications

Publications (598)
Article
A novel membraneless β-glucan/O2 enzymatic fuel cell was developed by combining a bioanode based on buckypaper modified with co-immobilized Agaricus meleagris pyranose dehydrogenase (AmPDH) and Rhodothermus marinus β-glucosidase (RmBgl3B) (RmBgl3B-AmPDH/buckypaper) with a biocathode based on solid graphite modified with Myrothecium verrucaria bilir...
Article
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Pure hydrocarbons with shape and conjugation properties that can be switched by external stimuli is an intriguing prospect in the design of new responsive materials and single-molecule electronics. Here, we develop an oligomeric [8]annulene-based material that combines a remarkably efficient topological switching upon redox changes with structural...
Article
Supercapacitive biofuel cells’ (SBFCs) most recent advancements are herein disclosed. In conventional SBFCs the biocomponent is employed as the pseudocapacitive component, while in self-charging biodevices it also works as the biocatalyst. The performance of different types of SBFCs are summarized according to the categorization based on the biocat...
Article
Full-text available
Extra charges in semiconductor nanocrystals are of paramount importance for their electrically driven optoelectronic and photovoltaic applications. Optical excitations of such charged nanocrystals lead to rapid recombination via an Auger process, which can deteriorate the performance of the corresponding devices. While numerous articles report trio...
Article
Herein, we present a novel electrode platform for H 2 O 2 detection based on the immobilization of recombinant Tobacco Peroxidase (r‐TOP) onto graphite electrodes (G) modified with p ‐phenylenediamine ( p ‐PD) diazonium cation grafted multi‐walled carbon nanotubes (MWCNTs). The employment of both p ‐phenylenediamine moieties and covalent cross‐link...
Article
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Fulfilling the potential of colloidal semiconductor quantum dots (QDs) in electrically driven applications remains a challenge largely since operation of such devices involves charged QDs with drastically different photophysical properties compared to their well-studied neutral counterparts. In this work, the full picture of excited state dynamics...
Article
Herein, we present an alternative approach to obtain a highly sensitive and stable self-powered biosensor that was used to detect D-fructose as proof of concept.In this platform, we perform a two-step process, viz. self-charging the biosupercapacitor for a constant time by using D-fructose as fuel and using the stored charge to realize the detectio...
Preprint
Full-text available
Fulfilling the potential of the colloidal semiconductor quantum dots (QDs) in electrically driven applications remains a challenge largely since operation of such devices involves charged QDs with drastically different photo-physical properties compared to their well-studied neutral counterparts. In this work, the full picture of excited state dyna...
Article
Herein this special issue of Bioelectrochemistry on the sugar oxidizing enzymes a large number of publications are found written by invited experts in this field. Glucose oxidase and NAD-dependent glucose dehydrogenase are examples of enzymes well-known to the (bio)electroanalytical community but also other enzymes not so well-known are reviewed su...
Article
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The transwell assay is currently the most popular approach to studying cellular invasion due to its ease of use and readout, and the possibility for quantitative measurements. However, it only allows end-point measurements without the possibility for real-time tracking of the dynamics of cell movement during an invasion. Moreover, it requires cell...
Article
A major challenge in the design of electrochemical biodevices is to achieve fast rates of electron exchange between proteins and electrodes. In this work, we show that a significant increase in the direct electron transfer rate between a graphite electrode and Tobacco Peroxidase takes place when a surface exposed leucine, located in the vicinity of...
Article
Titanium dioxide (TiO2) is a unique material for biosensing applications due to its capability of hosting enzymes. For the first time we show that TiO2 can accumulate reactive oxygen species (ROS) under daylight irradiation and can support the catalytic cycle of horseradish peroxidase (HRP) without the need of H2O2 to be present in the solution. Ph...
Article
Enterococcus faecalis cells are known to have ferric reductase activity and the ability to transfer electrons generated in metabolism to the external environment. We have isolated mutants defective in ferric reductase activity and studied their electron transfer properties to electrodes mediated by ferric ions and an osmium complex modified redox p...
Article
Full-text available
Changes in the tertiary conformation of adsorbed biomolecules can induce detectable shifts (Δθ r ) in the surface plasmon resonance (SPR) angle. Here it is shown how to calculate the corresponding shifts in the adsorbate's center of mass (Δzavg) along the sensing surface normal from the measured Δθ r . The novel developed model was used for determi...
Chapter
During the past few decades, intensive research has been focused on the application of biological photosynthetic processes for "green" electrical power generation. In this contribution, we consider different possible photobiocatalysts, such as isolated photosystems, thylakoid membranes (TMs), and whole photosynthetic organisms suitable for building...
Article
A combination of supercritical carbon dioxide (scCO2) impregnation of pyrrole and sonochemical transformation of permanganate (KMnO4) was used to impart conductive and catalytic properties to silk fibers. The results indicated that the conductivity (from polypyrrole -PPy) and catalytic activities (from manganese dioxide -MnO2) were independent and...
Article
During the few past decades, intensive efforts have been focused toward optimizing ‘wiring’ of thylakoid membranes (TMs) to conductive surfaces for their further application for light-energy conversion and production of an electron flow. This review summarizes recent progress and current state of photobioelectrodes and systems based on TMs. We cons...
Article
Full-text available
Background: Laccases are multicopper oxidases, which are assigned into auxiliary activity family 1 (AA1) in the CAZy database. These enzymes, catalyzing the oxidation of phenolic and nonphenolic substrates coupled to reduction of O2 to H2O, are increasingly attractive as eco-friendly oxidation biocatalysts. Basidiomycota laccases are well characte...
Article
To study the direct electron transfer (DET) of the multi-cofactor enzyme cellobiose dehydrogenase (CDH) in regard to its orientation on an electrode surface, a recently published, maleimide-based immobilization method was used in combination with site-directed mutagenesis to establish different orientations on an electrode surface. CDH from Myrioco...
Article
Graphite electrodes were modified with triangular (AuNTrs) or spherical (AuNPs) nanoparticles and further modified with fructose dehydrogenase (FDH). The present study reports the effect of the shape of these nanoparticles (NPs) on the catalytic current of immobilized FDH pointing out the different contributions on the mass transfer-limited and kin...
Article
Fructose dehydrogenase (FDH, EC 1.1.99.11) is a bacterial membrane bound flavocytochrome oxidoreductase, which has been widely employed to develop direct electron transfer (DET) and mediated electron transfer (MET) based electrode platforms [1]. Most commonly FDH from Gluconobacter japonicus has been studied. FDH is a heterotrimer consisting of a c...
Article
Full-text available
Application of enzymatic biofuel cells (EBFCs) in wearable or implantable biomedical devices requires flexible and biocompatible electrode materials. To this end, freestanding and low-cost graphene paper is emerging among the most promising support materials. In this work, we have exploited the potential of using graphene paper with two-dimensional...
Article
Electroactive microorganisms possess the unique ability to transfer electrons to or from solid phase electron conductors, e.g., electrodes or minerals, through various physiological mechanisms. The processes are commonly known as extracellular electron transfer and broadly harnessed in microbial electrochemical systems, such as microbial biosensors...
Article
In article number 1900215, Lo Gorton and co‐workers demonstrate that high molecular weight polymeric electron mediators are able to penetrate into the envelope of microbial cells and directly connect to their metabolism to create and enhance a conductive pathway to electrodes. The conceptual understanding of the processes explored in this study are...
Article
A combination of photosynthetic biocatalysts with high surface area conductive materials mediated by an osmium-complex modified redox polymer (OsRP) holds promising features for the development of sustainable “green” systems for solar energy conversion. In this work we performed a comparative study of two types of carbon nanotubes (CNTs) synthesize...
Article
We report on a hybrid bioelectrochemical system that integrates an energy converting part, viz. a glucose/oxygen enzymatic fuel cell, with a charge-storing component, in which the redox features of the immobilized redox protein cytochrome c (cyt c) were utilized. Bilirubin oxidase and pyrroloquinoline quinone-dependent glucose dehydrogenase (PQQ-GD...
Article
Non-turnover voltammetry is a sensitive tool to characterize the electrochemical properties of redox proteins. However, the catalytically competent oxidation states of most peroxidases do not display the required electrochemical reversibility. In this report, we circumvent this limitation and exploit the voltammetric response associated with the Fe...
Article
Employment of redox polymers as mediators is a promising concept to facilitate electron transfer (ET) and improve operational performance of bioelectrochemical systems. Materials science offers a broad range of mediation possibilities; however, their employment so far relies on a trial‐and‐error approach, since there is no comprehensive understandi...
Article
In the present study, the effect of carbon nanotubes (CNTs) either single walled (SWCNT) or multi walled (MWCNT) on sugar oxidation by Corynascus thermophilus cellobiose dehydrogenase (CtCDH) is investigated. The current observed for the direct electron transfer (DET) communication between the enzyme and the electrode is compared to that of the med...
Article
Full-text available
Pyranose dehydrogenase (PDH) is a quinone‐dependent extracellular flavoglycoprotein mainly produced by litter‐decomposing fungi and contributes to the degradation of lignocellulose. PDH in terms of structure and catalytic features pertains to the glucose methanol–choline oxidoreductase family and oxidizes a wide substrate range of aldopyranoses inc...
Article
In this paper, an efficient direct electron transfer (DET) reaction was achieved between fructose dehydrogenase (FDH) and a glassy carbon electrode (GCE) onto which anthracene modified single walled carbon nanotubes were deposited. The SWCNTs were in situ activated with a diazonium salt synthesized through the reaction of 2-amino anthracene with Na...
Article
We present a fuel-independent self-charging biosupercapacitor comprising an oxygen reducing enzymatic biocathode and an opposing bioelectrode, where the supercapacitive properties of immobilized protein were utilized. Our findings disclose a novel...
Article
In this paper we present a new method to electrodeposit highly porous gold (h-PG) onto a polycrystalline solid gold elec-trode without any template. The electrodeposition is carried out by first cycling the electrode potential between +0.8 and 0 V in 10 mM HAuCl4 with 2.5 M NH4Cl and then applying a negative potential for the production of hydrogen...
Article
Full-text available
Extracellular electron transfer (EET) in microbial cells is essential for certain biotechnological applications and contributes to the biogeochemical cycling of elements and syntrophic microbial metabolism in complex natural environments. The Gram-positive lactic acid bacterium Enterococcus faecalis, an opportunistic human pathogen, is shown to be...
Article
The interaction between microorganisms, that contain thick cell walls such as the Gram‐positive bacterium Enterococcus faecalis, and conductive surfaces can be improved using redox polymers as mediators. Here we report on a detailed electrochemical characterization of the communication between E. faecalis cells and a series of osmium redox polymers...
Article
Current research on enzyme based electrochemical biosensors deals essentially with the same target analytes as was at focus in the early days of biosensor research, that is those within the clinical/medical, food/agriculture, and environmental fields [1•–13]. However, there has been substantial progress through the years and progress continues, as...
Article
Harvesting the energy generated by photosynthetic organisms through light-dependent reactions is a significant step towards a sustainable future energy supply. Thylakoid membranes are the site of photosynthesis, and thus particularly suited for developing photo-bioelectrochemical cells. Novel electrode materials and geometries could potentially imp...
Article
In the present study, wild type and three mutants of pyranose 2‐oxidase (PyOx‐WT, PyOx‐MT1, PyOx‐MT2, PyOx‐MT3), which showed improved properties for D‐galactose oxidation, were investigated for their oxidising ability when immobilised on graphite electrodes. Four different flexible Os polymers with formal potentials ranging between −0.140 and 0.27...
Article
Full-text available
Dehydrogenase based bioelectrocatalysis has been increasingly exploited in recent years in order to develop new bioelectrochemical devices, such as biosensors and biofuel cells, with improved performances. In some cases, dehydrogeases are able to directly exchange electrons with an appropriately designed electrode surface, without the need for an a...
Article
In this work we report on the increase in photocurrent obtained by using thylakoid membranes “wired” with an osmium redox polymer (OsRP) immobilized onto screen-printed carbon and gold electrodes (SPCEs and SPAuEs), modified with gold microparticles (AuMPs) and gold nanoparticles (AuNPs). Both AuMPs and AuNPs were electrodeposited by using the same...
Article
Full-text available
We report on the influence of pH and monovalent/divalent cations on the catalytic current response, internal electron transfer (IET), and structure of fructose dehydrogenase (FDH) by using amperometry, spectrophotometry, and circular dichroism (CD). Amperometric measurements were performed on graphite electrodes, onto which FDH was adsorbed and the...
Article
Combination of thylakoid membranes (TMs) as photobiocatalysts with high surface area electroactive materials could hold great potentials for sustainable “green” solar energy conversion. We have studied the orientated immobilization of TMs on high surface area graphene electrodes, which were fabricated by electroreduction of graphene oxide and simul...
Article
In this work, a carbon felt electrode (CFE) was modified with a multiwalled carbon nanotube-gold-platinum (MWCNT−Au-Pt) hybrid nanomaterial and integrated with an osmium redox polymer (OsRP, [Os(2, 2’-bipyridine)2(poly-vinylimidazole)10Cl]Cl) and Gluconobacter oxydans DSM 2343 (G. oxydans) cells. The developed electrode was used as the bioanode in...
Article
Cellobiose dehydrogenase (CDH) is a versatile bioelectrocatalyst lately at focus due to its sugar oxidising properties in combination with its inherent ability for direct electron transfer communication with electrodes making it possible to be used in bioanodes in the enzymatic fuel cells (EFCs), self-powered biosensors, and biosupercapacitors. Dur...
Article
A comparison was made between two plant peroxidases, cationic horseradish peroxidase (HRP) and anionic tobacco peroxidase (TOP), combined with a highly cationic osmium polymer [Os(4,4'-dimethyl-2,2'-bipyridine)2poly(N-vinylimidazole)10Cl]+2/+ ([Os(dmp)PVI]+/2+) to develop highly sensitive, stable and selective hydrogen peroxide biosensors. The two...
Article
Integrating photosynthetic cell components with nanostructured materials can facilitate the conversion of solar energy into electric power for creating sustainable carbon-neutral energy sources. With the aim at exploring efficient photoinduced biocatalytic energy conversion systems, we have used an amidated carbon nanotube (aCNT) networked matrix t...
Article
This paper presents a new direct electron transfer based-miniaturized glucose/oxygen enzymatic fuel cell (EFC) whose operating ability has been tested in real saliva samples. The bioanode and biocathode are a graphene working electrode and a graphite counter electrode localized on the same screen printed electrode (SPE) modified with poly(vinyl alc...
Article
Lipidic cubic phase systems (LCPs) are excellent carriers for immobilized enzymes due to their biocompatibility and well-defined nanoporous structure. Lipidic cubic phases act as a convenient matrix to incorporate enzymes and hold them in the vicinity of electrode surfaces in their fully active forms. Corynascus thermophilus cellobiose dehydrogenas...
Article
Studying the interaction of functional proteins such as enzymes and nanoparticles (NPs) includes the important topic of investigating any possible changes in stability and function of enzymes in nanostructured environments. The effects of NPs on the enzyme activity and stability are governed by their physical and chemical properties such as structu...
Article
Over the past decades great attention has been paid to the phenomenon of microorganisms’ ability to transfer electrons between their metabolism and solid conductive surfaces. This fact keeps potential to gain extensive reliance for applications including electricity production coupled to wastewater treatment, bioremediation, and synthesis of value...
Article
Efficient direct electron transfer (DET) between a cellobiose dehydrogenase mutant from Corynascus thermophilus (CtCDH C291Y) and a novel glassy carbon (GC)-modified electrode, obtained by direct electrodeposition of gold nanoparticles (AuNPs) was realized. The electrode was further modified with a mixed self-assembled monolayer of 4-aminothiopheno...
Article
In this work, we have developed for the first time a method to make novel gold and platinum hybrid bimetallic nanostructures differing in shape and size. Au-Pt nanostructures were prepared by electrodeposition in two simple steps. The first step consists of the electrodeposition of nanocoral Au onto a gold substrate using hydrogen as a dynamic temp...
Article
Full-text available
Gluconobacter oxydans (G. oxydans) cells together with an osmium redox polymer (ORP) [Osmium (2,2’-bipyridine)2(poly-vinylimidazole)10Cl]Cl were combined with a glassy carbon paste electrode (GCPE) to form a bioanode for a microbial fuel cell (MFC) based on G. oxydans. Although there are G.oxydans/ ORP combined bioanode in the literature, as far as...
Article
The concept of supercapacitive photo-bioanode and biosolar cell (photo-biosupercapacitor) for simultaneous solar energy conversion and storage is demonstrated for the first time. Exploiting the capacitive component significantly improves the electron transfer processes and allows the achievement of a current density of 280 µA cm⁻² in the pulse mode...
Article
Full-text available
In article number 1602285, Sergey Shleev, Lo Gorton, and co-workers report on a novel concept of supercapacitive photobioanodes and bio-solar cells (photo-biosupercapacitors). Employment of a charge-storing component facilitates the electron transfer processes occurring in the system and significantly increases the photocurrent output. This strateg...
Article
Efficient conjugation between biomolecules and electrode materials is one of the main challenges in the field of biosensors. Cellobiose dehydrogenase (CDH) is a monomeric enzyme, which consists of two separate domains: one catalytic dehydrogenase domain (DHCDH) carrying strongly bound flavin adenine dinucleotide (FAD) in the active site and a cytoc...
Article
Full-text available
The front cover artwork is provided by Prof. Lo Gorton from Lund University (Sweden) and his co-workers. The image shows mutated cellobiose dehydrogenase (CDH) immobilized on a graphite electrode and how preferentially glucose is oxidized by this enzyme. Read the full text of the Article at 10.1002/celc.201600781.
Article
Full-text available
The Front Cover picture shows mutated cellobiose dehydrogenase (CDH) immobilized on a graphite electrode and how preferentially glucose is oxidized by this enzyme. The enzyme consists of a larger catalytic dehydrogenase domain (grey) containing FAD as cofactor (orange) and a smaller cytochrome domain (lavender) containing heme as cofactor (yellow)...
Article
Invited for this month's cover is the group of Prof. Dr. Lo Gorton at Lund University, Sweden. The cover picture shows a biosensor for measuring lactose in dairy products. It consists of a gold electrode, onto which a layer of polyethyleneimine-capped gold nanoparticles is deposited. Read the full text of the article at 10.1002/cplu.201600453.
Article
Biological photovoltaics (BPVs) are emerging as a potential sustainable energy-generating technology to convert solar energy into electrical energy. Although a great variety of photosynthetic biomaterials were studied in BPVs, cyanobacteria are considered as superior candidates because of their simpler physiology. To facilitate extracellular electr...
Article
Pyranose Dehydrogenase 1 from the basidiomycete Agaricus meleagris (AmPDH1) is an oxidoreductase capable of oxidizing a broad variety of sugars. Due to this and its ability of dioxidation of substrates and no side production of hydrogen peroxide, it is studied for use in enzymatic bio-fuel cells. In-vitro deglycosylated AmPDH1 as well as knock-out...