Ljiljana P. Stanojević

• PhD
• Full professor at University of Niš, Faculty of technology, Bulevar Oslobodjenja 124, Leskovac

This study examines the effect of extraction techniques (maceration-M, reflux-RE, ultrasonic-UE, Soxhlet extraction-SE) on the yield of total extractive matter (TEM), extraction kinetics, and antioxidant activity of black pepper fruits ethanolic extracts (BPFEEs). The content of total phenols and flavonoids was determined by Folin-Ciocalteu and AlC...

The purpose of this study was to examine differences between postharvest treatments, either washed (hot water, H2O2 and Na2OCl) or non-washed (control) carrot roots and the effect of different storage conditions, S1 (0°C and > 95% RH) or S2 (0-2°C and < 90% RH) on the compositional changes. Losses of mass, Beta-carotene and vitamin C in carrot tapr...

The aim of the research was to determine the yield, chemical composition and free radical-scavenging activity of the essential oils (EOs) in the leaves and fruit of wild-grown bay (Laurus nobilis L.) and rosemary(Rosmarinus officinalis L.)from the Montenegro coast. The bay essential oil (BEO) yield was 0.88% in fruit and 2.65% in the leaves. The ro...

This study was conducted to determine the sea fennel essential oil (SFEO) yield, composition , and antioxidant activity of leaves, stem, inflorescences, and umbels from seeds of wild sea fennel (SF) (Crithmum maritimum L.) from the Montenegro coast. The chemical composition of isolated essential oil was determined by GC/MS and GC/FID analyses. The...

Antioxidant activity of hydrolate obtained from the aerial part of sweet basil Ocimum basilicm L. Ocimum basilicum is a well-known aromatic, medicinal, and culinary plant. The essential oils obtained through steam distillation from the leaves and flowering tops of sweet basil have antiseptic, antimicrobial, antioxidant, antiviral, and anti-inflamma...

Citation: Milenković, L.; Ilić, Z.S.; Stanojević, L.; Danilović, B.; Šunić, L.; Kevrešan, Ž.; Stanojević, J.; Cvetković, D. Chemical Composition and Bioactivity of Dill Seed (Anethum graveolens L.) Essential Oil from Plants Grown under Shading. Plants 2024, 13, 886. https://doi. Abstract: This study determined the content and composition of dill se...

The increased interest in natural, renewable, biodegradable, easily available, low-cost materials makes agricultural residues, such as lignocellulosic biomass, attractive raw materials for the preparation of effective biosorbents for various pollutants (metal ions, anions, dyes, pharmaceutical degradation products, metabolites, organic macromolecul...

The present study aimed to compare phytochemical properties (total extractive matter, phenolic, flavonoids, and mineral content), antimicrobial and antioxidant activities of black pepper fruits' ethanolic extract (BPFEE), and cubeb pepper fruits' ethanolic extract (CPFEE). The extracts were prepared by reflux extraction under identical conditions (...

This study was conducted to investigate the effect of plant shading (covered by pearl shade nets with a 40% shade index or nonshading plants from open field) on the content and composition of essential oils extracted from summer savory (Satureja hortensis L.) during the flowering stage cultivated under the ecological conditions of Serbia. The summe...

This study was conducted to investigate the effect of plant shading (covered by pearl shade nets with a 40% shade index or nonshading plants from open field) on the content and composition of essential oils extracted from summer savory (Satureja hortensis L.) during the flowering stage cultivated under the ecological conditions of Serbia. The summe...

(1) Background: Vulvovaginal candidosis (VVC) is a major therapy issue due to its high resistance rate and virulence factors such as the ability to form biofilms. The possibility of combining commonly used antifungals with natural products might greatly improve therapeutic success. (2) Methods: A total of 49 vulvovaginal isolates, causative agents...

Chemical profiling the sage essential oils (SEOs) from wild and cultivated (shaded or non-shaded) plants has been investigated. The yield of SEOs from wild plants (3.51 mL/100 g) was higher than that from cultivated plants(shaded plants: 3.20 mL/100 g and non-shaded plants: 2.56 mL/100 g).The main components of SEO from wild plants were cis-thujone...

This work aimed to evaluate chemical profile, antioxidant activity and topical application safety of the raw Osage orange (Maclura pomifera (Raf.) Schneid) fruit extracts obtained by maceration using ethanol and acetone. Out of eighteen different compounds registered in the extracts, fifteen were identified by ultra-high-performance liquid chromato...

The study was conducted to determine fennel essential oil (FEO) yield, composition, and antioxidant activity during four different maturation stages of umbels with seeds (1st stage: immature-pasty; 2nd stage: premature-waxy; 3rd stage: mature-fully ripe; and 4th stage: seeds only), and leaves of wild fennel (Foeniculum vulgare Mill. subsp. vulgare)...

The purpose of this study was to determine essential oil yield, composition, and antioxi-dant activity during two different maturation stages of umbels with seeds(I stage: premature-waxy; and II stage: mature-fully ripening) of wild carrot (Daucus carrota var. carota) from the Montene-grin coast. A higher yield of carrot essential oil (CEO) was det...

Thyme, mint, and lemon balm were used to determine whether shading conditions could improve the yield, composition, antioxidant, and antimicrobial activity in plant essential oils (EOs) in comparison with non-shaded plants from an open field. The yield of the EOs of non-shaded thyme, mint, and lemon balm, was 3.44, 3.96, and 0.21 mL/100 g, respecti...

Introduction. Lemon balm has been used for centuries due to its strong sedative, antimicrobial and spasmolytic effects. Besides different types of extracts and essential oils, hydrolates (by-products obtained during essential oil extraction) are preparations of aromatic medicinal plants with potential pharmacological activity. Aim. To estimate Meli...

The objective of this study was to analyze the chemical composition of the essential oil (EO) and hydrolate of black pepper fruit, as well as their antioxidant activity. The EO was obtained by Clevenger-type hydrodistillation with hydromodule 1:10 m/v for 240 minutes, and the hydrolate was collected after the hydrodistillation process. The qualitat...

The present study aimed to determine and compare chemical composition and antioxidant activity of essential oils (EOs) isolated from marigolds (Tagetes patula L.) cultivated in the garden in southeast Serbia. The EOs were isolated from dry orange and red flower petals by Clevenger type hydrodistillation during 2 h by using 1:15 m/V hydromodule. The...

This study examines the action of light modifications by different shade nets (pearl, red and blue color nets with a shade index of 50%) in relation to unshaded plants from the open field on the relative effects on changing basil aroma profile and antioxidant activity. The most abundant components were linalool, 1,8-cineole and myrcene. Aqueous ext...

The present study focuses on the yield, chemical composition, and antioxidant activity of essential oils from different parts (flowers or leaves/stems) of cultivated plants grown under pearl shade nets with a 40% shaded index or in nonshaded plants and wild-grown oregano. The chemical composition of isolated essential oils was determined by GC/MS a...

The aim of this work was to compare the chemical composition and antioxidant activity of the essential oils from two plant parts (leaves and stems) of fennel, wild-grown in the Montenegro seaside. The chemical composition of the isolated essential oils was determined by gas chromatography with mass spectrometry (GC/MS) and flame ionization detectio...

The present study aimed to determine the chemical composition and antioxidant activity of immortelle (Helichrysum italicum (Roth) G. Don) and yarrow (Achillea millefolium L.) essential oils isolated from the aerial plant parts on a semi-industrial scale by steam and water-steam distillation, respectively. The qualitative composition of the essentia...

Bacteriochlorins as the porphyrins derivatives are well known photosensitizers with great potential for use in various fields of pharmacy and medicine. Photostability of selected bacteriochlorins, bacteriochlorophyll a and bacteriopheophytin a, in different methanol solutions (with and without lipids) during continual UV-A, UV-B and visible light t...

The aim of the presented work was to compare the efficiency of innovative (solvent-free microwave extraction, microwave-assisted hydrodistillation and supercritical CO2 extraction) extraction techniques vs. conventional Clevenger hydrodistillation of essential oil (EO) from poorly studied fennel rhizome. It was found that innovative extraction tech...

Thyme, marjoram, lemon balm, mint, and sweet basil were used to determine whether light modification (plants grown under pearl nets with 50% shaded index or un-shaded-open field conditions) could improve the antimicrobial activity of essential oils (EOs). Obtained results were discussed concerning previously determined yield and composition of EOs...

The aim of this study is to investigate the possibility of using electrospun polylactide (PLA) fibers as a carrier of the phytoestrogen biochanin A. Polylactide fibers were prepared with different contents of biochanin A by using an electrospinning method at specific process parameters. The obtained electrospun polylactide fibers, as carriers of bi...

Lemon balm, mint, and basil were used to determine whether light modification (plants grown under pearl shade nets with 40 % shaded index or in un-shaded open field) could improve the quantity and quality of essential oils (EOs) and antioxidant activity. The yield of EOs of lemon balm, mint and sweet basil obtained after 120 min of hydrodistillatio...

The manipulation of light intensity by shade nets can lead to exchanges in the phytochemical quality and antioxidants ofsome herbs. This study aimed to determine whether shading by pearl nets (50% shade index) could improve the bioactive compounds in several medicinal herbs such as thyme (Thymus vulgaris L.), marjoram (Origanum majorana L.), oregan...

The aim of the presented work was to evaluate polar and non-polar extracts and fatty oil, isolated from pumpkin fruit (Cucurbita pepo L.), as a potential food supplements or raw material for cosmetic and pharmaceutical preparations. The extracts were isolated from the pumpkin peel, mesocarp and pulp and fatty oil from the seeds. The highest yield o...

The aim of this study was to determine the antimicrobial activity of essential oils obtained from sweet basil (Ocimum basilicum L. cv. ‘Genovese’) cultivated in the open field under different shading conditions (red, blue, and pearl nets with a shade index of 50% and full sunlight exposure (control plants)), harvested at different times. The antimi...

Thymus vulgaris L. (thyme), Origanum majorana L. (marjoram), and Origanum vulgare L. (oregano) were used to determine whether light modification (plants grown under nets with 40% shaded index or in un-shaded open field) could improve the quantity and quality of essential oils (EOs) and antioxidant activity. The yield of EOs of thyme, marjoram, and...

This work aimed to isolate the essential oil from the nutmeg (Myristica fragrans Houtt.) seed, to determine the qualitative and quantitative chemical composition of the obtained essential oil, as well as to examine its antioxidant and antimicrobial activity. The essential oil was obtained by Clevenger-type hydrodistillation from the disintegrated n...

Essential oil from rosemary (Rosmarinus officinalis L.) leaves was isolated by conventional hydrodistillation using hydromodulus of 1/15 m/v and by distillation after the plant material pretreatment with 10% v/v aqueous solution of natural deep eutectic solvent glyceline. Qualitative and quantitative composition of the oil was determined by GC/MS a...

For quantitative analysis, contents of the selected three phenolic compounds and 17 minerals were determined in seven tea samples, i.e., infusions prepared on a traditional way from the dried aerial parts (herba) of St. John’s wort (Hypericum perforatum L.) found in the market, or collected from few locations in the Eastern and Southeastern Serbia....

The temperature- and pH-responsive poly(N-isopropylacrylamide-co-acrylic acid), p(NIPAM-co-AA), copolymer was synthesized by free radical polymerization and examined as a carrier for modified release of biochanin A. Biochanin A is a biologically active methoxylated isoflavone which exhibits estrogenic and other pharmacological activities. Due to it...

The aim of the presented work was to investigate the potential of aqueous extract of cultivated strawberry (Fragaria x ananassa Duch.) leaves for stabilization of silver nanoparticles (AgNPs-E) synthesized at room (RT) and boiling temperature (BT). The synthesis and stability of AgNPs-E were monitored by UV-Vis spectroscopy confirming high stabilit...

Black pepper (Piper nigrum L.) is a tropical plant, best known for its fruit, used as a spice all around the world. The fruits of black pepper can be processed in various ways, so there are end products such as white, black, red, and green pepper. Black pepper contains many substances such as terpenes, alkaloids, lignans, phenylpropanoids, etc., wh...

The quality of the essential oil obtained from white pine wood greenery from the territory of the Republic of Srpska was analyzed by examining the chemical composi- tion and antimicrobial activity of samples produced by the hydro-distillation process in industrial production conditions. The chemical composition of the essential oil was analyzed by...

The aim of this study was to isolate the essential oil from ripe and disintegrated caraway fruit by Clevenger-type hydrodistillation, to determine its qualitative and quantitative composition and to examine its antioxidant activity, as well as to determine the qualitative and quantitative chemical composition of the caraway volatile compounds extra...

The quality of the essential oil obtained from the wood verdure of fir from the territory of the Republic of Srpska was analyzed by examining the chemical composition, antioxidant activity and antimicrobial activity of the samples produced by the process of hydrodistillation in industrial production conditions. Two samples of fir essential oil were...

The objectives of this study were to investigate the effects of coloured shade-nets (pearl, blue, or red all with a 50% shade index) compared to non-shaded plants on quality traits on the Discoa and ICE 40102 (green-leaf) and Eglantine (red-leaf) lettuce cultivars. Total chlorophyll content depended on the shading and lettuce genotype. The chloroph...

The optimization of the headspace solid-phase microextraction in combination with the gas chromatography-mass spectrometry (HS-SPME-GC/MS) for the qualitative identification of the aroma compounds in fruit spirits (Williams, plum spirit, grape spirit , apricot spirit) was the subject of the presented paper. The SPME extraction conditions (temperatu...

BACKGROUND The need to increase marketable tomato yields and decrease losses due to sunburn and disease during the summer motivates farmers to adopt additional cultural practices such as shading and grafting. To investigate complex interactions among grafting, shading, and tomato cultivar, grafted and ungrafted tomatoes (cv. 'Optima' F1 and cv. 'Bi...

The purpose of the present study was to determine chemical composition and antioxidant activity of commercial peppermint (Aetheroleum menthae piperitae) and basil (Aetheroleum basilici) essential oils as well as their effects on skin biophysical parameters. The qualitative and quantitative composition of essential oils was determined by GC-MS and G...

The common birch (Betula pendula Roth., Betulaceae) is widespread in moderate and northern climatic zones. It is used in traditional medicine of many countries, and its positive effects on human health were recognized a long time ago. It belongs to a group of medicinal plants. Numerous studies on the chemical composition and activity of the birch i...

In the present work, fruits from two plant species, Rosa canina L. and Sorbus aucuparia L., popular in traditional folk medicine in Serbia, were studied. The aim was to examine and compare the efficiency of the ultrasonic extraction with different solvents regarding physicochemical properties, polyphenolic profile of extracts, as well as their tyro...

The essential oil from birch (Betula pendula Roth.) buds was obtained by Clevenger-type hydrodistillation. Its qualitative and quantitative composition was determined by gas chromatography in combination with mass spectrometry (GC/MS) and flame ionization detection (GC/FID), respectively. Twenty-seven compounds, mainly sesquiterpene hydrocarbons (7...

Earth smoke herb (Fumaria officinalis L.) was used as a source of extractive matter in this study. The aqueous extract was obtained by pouring boiling water over the plant material (1:20 m/v) and letting it stay for 90 minutes at room temperature. The identification of bioactive compounds in the extract was performed using the UHPLC-DAD-ESI-MS anal...

Abstract. In order to develop a method for rapid, efficient and accurate detection and quantitative determination of gliadin proteins from wheat flour, the usage of different solvents and different separation conditions were investigated by reversed-phase high performance liquid chromatography (RP-HPLC). After albumin and globulins were removed fro...

Synthesis of silver nanoparticles using plant extract of Fumaria officinalis L., (AgNPs-E), as a reducing and capping agent, is presented in this study. Formation of AgNPs-E is monitored by measuring surface plasmon resonance (SPR) absorption band in the UV-VIS range 200-800 nm. Based on UV-VIS spectra , the SPR band of AgNPs-E obtained by synthesi...

Aronia (Aronia melanocarpa) leaves at the end of harvest season (in the leaves senescence vegetative stage), when it represents an agricultural waste material, have been extracted under different conditions in order to assess potential of this plant material in valuable bioactive compounds recovering. Response surface methodology was used in screen...

The subject of this study was the examination of the chemical composition and antimicrobial activity of peppermint oil (Menthae piperitae L.), that was produced from the plant collected from the plantation in the city of Banja Luka. To examine the chemical composition of the Mentha piperita essential oil we used the GC-FID method. On it’s chromatog...

Wild cyclamen tubers (Cyclamen purpurascens Mill.) (mountain Kukavica, Southeast Serbia) were used as material for extraction in this study. Aqueous extract was obtained by reflux extraction on boiling temperature with hydromodulus 1:20 m/v during 180 minutes. The total phenolic content was determined spectrophotometrically by the method of Folin-C...

University of Niš, Faculty of Technology, Leskovac, Serbia Wild cyclamen tubers (Cyclamen purpurascens Mill.) (Kukavica mountain, southeast Serbia) was used as an extraction material in this study. The aqueous extract has been obtained by reflux extraction at the boiling temperature with hydromodulus 1:20 m/v during 180 minutes. The identification...

In the last decade, agricultural producers in Serbia have been massively reoriented to aronia (Aronia) cultivation. Due to low and inadequately organized purchase, the accumulation of large amounts of aronia forced the producers to organize and start processing. The most common and most represented products of aronia in our market are cold pressed...

The effects of utilization of different color shade nets in lettuce production during the summer season were evaluated in Serbian climate conditions for pearl, blue, red and black shade nets in comparison to open field production. Applied shade nets (with shade index of 50%) significantly reduced solar irradiation (from 890 in open field to 400–560...

Essential oils, as sources of natural products, represent an alternative to synthetic antioxidants and antimicrobial agents in food and pharmaceutical industry, alternative medicine and natural therapy. Basil (Ocimum basilicum L.) belongs to the Lamiaceae family of herbs, which in addition to being used as a spice, is also medicinally known as a po...

Dichloromethane soil samples extracts were prepared using Soxhlet extraction technique, and after clean-up step, gas chromatography-mass spectrometry analysis of 16 priority polycyclic aromatic hydrocarbons (PAHs) was carried out. A comparison of dispersive solid-phase extraction (dSPE) and column chromatography (cC), as clean-up techniques, was ev...

Antioxidant activity of aqueous extracts from two cultivated strawberry leaves, Senga Sengana and Mount Everest, obtained by maceration, reflux extraction and ultrasonic extraction, was investigated. Antioxidant activity of extracts was assessed by DPPH, FRAP, FIC and H2O2 test; total phenolic and flavonoids by Folin-Ciocalteu and aluminium chlorid...

The subject of this study is to determine chemical composition, antioxidant and antimicrobial activity of essential oil obtained from chamomile flowers (Matricaria chamomilla L.). The essential oil has been obtained by hydrodistillation process on semi industrial production conditions. Qualitative and quantitative composition of the oil was determi...

Synthetic antioxidants and antimicrobial agents can induce many undesired side effects, which attracts interest of food producers and consumers in finding ingredients of natural origin. The antioxidative and antimicrobial activity of essential oil from dill (Anethum graveolens L.) seeds was investigated in terms of its possible application as natur...

Five extraction techniques, maceration, reflux, Soxhlet, Tillepape and ultrasonic extraction, were used to obtain the extractive matter from nettle leaves. The antioxidant activity of extracts was assessed by: DPPH, FRAP and H2O2 test, while the total phenolic and total flavonoid content was determined according to the Folin-Ciocalteu and aluminium...

Strawberry is a member of the rose family (Rosaceae, subfamily Rosoideae, tribe Potentilleae) in the genus Fragaria. The cultivated varieties of commercial strawberries usually were designated as Fragaria ananassa. Root, leaf, flower and fruit have the healing properties. The strawberry leaves extract is used for blood cleaning, for treatment of or...

This study investigates the biological activity of pure allantoin (PA) and aqueous extract of the comfrey (Symphytum officinale L.) root (AECR) standardized to the allantoin content. Cell viability and proliferation of epithelial (MDCK) and fibroblastic (L929) cell line were studied by using MTT test. Anti-irritant potential was determined by measu...

Allicin is the most biologically active substance present in garlic. It can be synthesized or obtained by extraction of fresh garlic. Transformation products of allicin are also biologically active. The aim of this study was to examine the antioxidant activity of synthesized allicin and its transformation products obtained using microwaves in metha...

In the present work, essential oil has been obtained by Clevenger-type hy-drodestillation from grounded curcuma rhizome (Rhizoma Curcumae) (Turkey) with hydromodulus 1:5 m/V during 180 minutes. The qualitative and quantitative composition of the oil was determined by GC-MS and GC-FID spectrom-etry. The antioxidant activity of the obtained oil was d...

Antioxidant and antimicrobial activities of a new phyto-gel were studied in this work. The formulated gel is composed of propilene glycol extracts of St. John's worth (H. perforatum L.), comfrey (S. officinale L.), yarrow (A. millefolium L.), horsetail (E. arvense L.) and Na-usniate. The antioxidant activity was determined for the gel and each inve...

The present paper deals with the fermentation of chopped, dried, woolly foxglove foliage, the extraction of secondary glycosides from fermented woolly foxglove foliage by the percolation method and the isolation and purification of digoxin. Optimal process conditions for fermenting the chopped, dried, woolly foxglove foliage, extracting of digoxin...