Lisiane Almeida Martins

• DR.
• Professor at FACULDADE DE ENSINO SUPERIOR SANTA BÁRBARA

Atualmente, o gênero Staphylococcus spp. é considerado um dos principais patógenos que causam infecções em seres humanos e animais, e o problema atual é que os novos medicamentos antimicrobianos não acompanharam o número crescente de cepas bacterianas resistentes. Devido a essa situação, terapias complementares usando extratos botânicos com caracte...

This study aimed to determine the phenotypic, molecular and epidemiological profile of methicillin resistant Staphylococcus spp. in pregnant women. Were included 100 asymptomatic pregnant women between 16 and 38 years old, who underwent microbiological examination by collecting a vaginal swab at the first trimester of pregnancy. The isolates were s...

Staphylococcus spp. are among the main pathogens responsible for important infections worldwide, both in humans and in domestic or wild animals. Chiropterans, popularly known as bats, inhabit natural environments and human constructions, and have been considered potential disseminators of several pathogens. Studies have shown the expression of the...

Background: The objective of this study was to isolate Staphylococcus spp. and to characterize the resistance profile in nasal samples from pigs slaughtered for consumption. Material and Methods: Intranasal swabs were collected from 100 pigs immediately after bleeding in a slaughterhouse located in the largest pork production region in Brazil, thes...

Taking into account the increasing search for quality milk production, the objective was to evaluate the milk quality of 33 properties located in the northwest region of Paraná in 2018, relating them to current legislation (normative instruction 76). The results of standard plate count (SPC), somatic cell count (SCC), fat, non-fat solids, total sol...

The presence of Staphylococcus spp. resistant to methicillin in the nasal cavity of swine has been previously reported. Considering the possible occurrence of bacterial resistance and presence of resistance genes in intensive swine breeding and the known transmissibility and dispersion potential of such genes, this study aimed to investigate the pr...

Pig farming is an area of livestock that has been developing the most in Brazil and the world, with production increasing every year, generating jobs, and being of great importance for the Brazilian economy. In swine production, great health enables these animals to reach their highest point of development and antimicrobials are used, either prophy...

Five male specimens of the species Molossus rufus from north‐western Parana were captured, identified, packaged and transported to the laboratory for weighing and later euthanasia with isoflurane. They were laparatomized for evaluation of macroscopic characteristics and the digestive tube segments were collected for fixation in 10% neutral formalin...

The aim of this study was to verify the bacterial resistance profile and detect the presence of mecA gene in Staphylococcus spp. isolated from the nasal microbiota of domiciled dogs. For this purpose 100 nasal swabs from 100 domiciled dogs were collected from the central area of the city of Umuarama (PR), along with a questionnaire answered by thei...

Bovine mastitis is mainly caused by bacteria of the genus Staphylococcus spp., which possess different virulence factors, including the capacity for biofilm formation that provides enhanced protection against the action of immune system components and serves as a barrier against the penetration of antimicrobial agents. This study aimed to character...

Background: Contact between humans and pets, mainly dogs and cats, has been increasing in recent years, which may result in the spread of infectious agents to new hosts and even to the environment, causing emergencies of national and international interest. The aim of this work was to understand the phenotypic profile of bacteria of the Enterobacte...

Plastic surgeries are considered clean or potentially contaminated procedures. The incidence of infection in reduction mammoplasty is 1.1 to 22% and the main etiological agents are bacteria found on the skin and mucous membranes such as Staphylococcus aureus. Due to the increase in bacterial resistance with the widespread use of antibiotics, identi...

Mastitis is a breast tissue disease with a high incidence in dairy cows and implications ranging from the health of the animals to the economy of the sector. Although antibiotic therapy is widely used, the search for new perspectives in the management and treatment of this disease is necessary. Although phage research preceded the discovery of anti...

Staphylococcus aureus (S. aureus) são agentes causadores de doenças em todo o mundo, que acarretam grandes prejuízos e riscos à saúde humana e animal. Isto ocorre devido à grande capacidade que este micro-organismo possui de adquirir genes que codificam a produção de biofilmes, toxinas e, principalmente resistência a antimicrobianos. Com isso, torn...

There are no studies that characterize the enteric nervous system (ENS) bats. The organization and density of myenteric neurons may vary according to the animal species, as well as the segment of the digestive tube considered. The nitric oxide is one of the key neurotransmitters present in the myenteric neurons, acting as a mediator in the smooth m...

The aim of this study was to evaluate the profile of antimicrobial resistance in members of the family Enterobacteriaceae and to detect strains of extended-spectrum beta-lactamases (ESBL) in free-living Sapajus nigritus. Oral and rectal swabs were collected from 15 primates to isolate and identify bacteria, conduct phenotypic tests to detect antimi...

Background: In Brazil, cats in households has recently increased dramatically, likely due to their lower space and care requirements. We need to know the health of these companion animal species, since they have behavioral patterns that make them an important link in the epidemiological chain. Extended spectrum beta-lactamase producer strains (ESBL...

The functionality of nutraceutical foods is attributed to their bioactive compounds. These compounds are widely produced by plants, such as phenolic compounds, which have antioxidant activity and/or antimicrobial activity, acting against damage to macromolecules such as lipids, proteins, and nucleic acids. Secondary plant metabolites, including cla...

Background: The indiscriminate use of antibiotics both in humans and in animals, has contributed to the development of bacterial resistance. One of the key mechanisms in the resistance of enterobacteria to antibiotics is the production of extended-spectrum β-lactamases (ESBLs), which reduce the therapeutic options available. Several studies have be...

Os micro-organismos presentes durante a ordenha podem ter uma grande redução quando os utensílios são corretamente higienizados e realiza-se o pré-dipping com produtos que possuem eficácia comprovada, evitando, assim, a proliferação de micro-organismos resistentes. O presente trabalho tem como objetivo verificar a eficácia dos principais desinfetan...

Populations of nonhuman primates are often considered to be a link in the chain of emerging infectious diseases, as they are reservoirs for different zoonotic pathogens. The objective of this study was to identify the presence of bacteria from the family Enterobacteriaceae in free-living nonhuman primates. The research was carried out in an urban p...

Background The indiscriminate use of antibiotics has caused bacteria to develop mechanisms of resistance to antibacterial agents, limiting treatment options. Therefore, there is a great need for alternative methods to control infections, especially those related to skin. One of the alternative methods is the high frequency equipment (HFE), which is...

O objetivo deste trabalho foi avaliar os padrões qualitativos dos recursos hídricos localizadas no Parque Municipal dos Xetás na cidade de Umuarama (PR), o local é considerado Área de Proteção Ambiental, onde possui algumas fontes de poluição gerando conhecimento das condições físico-químicas e bacteriológicas para os habitantes da cidade ou para o...

A hipertensão arterial tornou-se na atualidade um dos grandes problemas da saúde Pública em vários países, incluindo o Brasil e alterações nos hábitos, incluindo os hábitos alimentares são importantes para a prevenção, principalmente com a redução do consumo de sódio. Este trabalho objetivou desenvolver e analisar salames desenvolvidos com redução...

The use of antimicrobial agents has led to the emergence of resistant bacterial strains over a relatively short period. Furthermore, Staphylococcus spp. can produce β-lactamase, which explains the survival of these strains in a focus of infection despite the use of a β-lactam antibiotic. The aim of this study was to evaluate the resistance of Staph...

Foodborne illness results in high costs to public health and the food chain production worldwide. The majority of the population is unaware of food safety requirements and consequently, domestic kitchens are sources of contamination and spread of disease. Eating habits and the profile of food handlers in these sites guide the planning of surveillan...

Rev. Bras. Med. Vet., 38(Supl.1):79-84, junho 2016 79 RESUMO. A leptospirose é causada por bactérias patogênicas do gênero Leptospira e acomete diver-sas espécies animais, incluindo os humanos. Os animais portadores são considerados a principal fonte de transmissão da doença, pois as leptospi-ras se localizam nos túbulos renais e são excreta-das na...

A mastite é a principal enfermidade que acomete o gado leiteiro. De todas as formas, a mastite subclínica é a que causa maiores perdas, na redução da produção, na diminuição da qualidade do leite e pelos altos custos de tratamento e descarte precoce de vacas. Altas contagens de células somáticas e baixo teor de sólidos totais no leite geralmente es...

Leptospirosis is caused by pathogenic bacteria of the genus Leptospira, affecting several animal species, including humans. Carrier animals are considered the main source of transmission because these bacteria are located in the renal tubules and are excreted in the urine for a variable period of time. Due to the variety of clinical signs the lepto...

Epidemiological studies have identified Staphylococcus aureus as the most common agent involved in food poisoning. However, current research highlights the importance of toxigenic coagulase-negative staphylococci (CoNS) isolated from food. The aim of this study was to characterize Staphylococcus spp. isolated from cows with bovine subclinical masti...

This study aims to determine the physical and chemical characteristics of milk from southwestern Paraná region. To this end, 293 properties were classified according to the number of animals, was later made simple drawing of each class, making an average of nine animals and different characteristics regarding race, milking type and stage of lactati...

The present study aimed to elucidate the sanitary quality of artisanal sausage registered under Municipal Inspection and marketed in Paraná municipalities. Thirty-one samples were collected by the Municipal Sanitary Surveillance and sent to the Central Laboratory of the State - LACEN being distributed between Campo Largo (5), Campo Mourão (2) and G...

Tetradenia riparia (Hochst.) Codd (Lamiaceae) species is native of South Africa, and in Brazil it was introduced as an exotic ornamental plant and is already well-adapted to the climatic conditions. Its oil presents a complex structure of monoterpenes, sesquiterpepnes and deterpepnes with antimicrobial, antimicrobiana, antinociceptive and acaricide...

Bovine mastitis is the main cause of economic losses in dairy industry. The aim of this study was to determine the prevalence and antibiotic-resistance profile of Staphylococcus aureus and coagulase-negative Staphylococcus spp. isolated from bovine mastitis cases in the municipality of Altônia, northwest region of Paraná State, Brazil. Two hundred...

Mastitis is an infection which often commits animals assigned for milk production. It has been the major price raise factor for dairy cattle breeding. Economical losses impact on professional fees, drugs, death and early discard of the animal as well as dairy companies as a result of the downturn in product end-quality, decrease of industrial proce...

The aim of this study was to identify the presence of different parasites in synanthropic rodents captured in the urban and peri-urban area in the city of Umuarama, Parana (PR). The rodents were euthanized for measurement and collection of feces and ectoparasites. Specific identification keys were used for the ectoparasitological analysis and the t...

Mastitis is an infection which often commits animals assigned for milk production. It has been the major price raise factor for dairy cattle breeding. Economical losses impact on professional fees, drugs, death and early discard of the animal as well as dairy companies as a result of the downturn in product end-quality, decrease of industrial proce...

The electrodeposition of polypyrrole and polyaniline has been performed in aqueous solutions of sodium saccharinate and sulphuric acid, respectively. The coatings were obtained by voltamperometric and galvanostatic methods. The corrosion behaviour of polypyrrole and polyaniline coated stainless steel (SS) in sulphuric acid medium was investigated b...

Leptospirosis, toxoplasmosis and brucellosis are diseases with worldwide distribution. Among stray dogs, these zoonoses are facilitated by direct contact with other animal species, by the habit of scavenging garbage and hunting in search of food, drinking standing water, smelling other animals' urine, licking female genitalia and the sexual act its...

The effect of a dentifrice containing essential oil from Ocimum gratissimum L. on bacterial plaque, bacterial charge and dental calculus was evaluated in dogs with grade I periodontal disease. Sixteen dogs were randomly separated in two groups: GI (dentifrice with essential oil from Ocimum gratissimum L.) and GII (base). The dogs were examined befo...

Over the past 20 years, the poultry sector was the one that most invested in technology, which explains its evolution in terms of production. These gains were due to improved feed conversion ratios, breeding, increased automation and better management of aviaries. Salmonella spp. is a bacterium that inhabits both human being and animal intestinal t...

Bovine mastitis is considered a major problem in the dairy farms, due to provokes large economic losses, well as damage to public health, through the placement of microorganisms in milk. The diagnosis is based on tests that verify the leukocyte content of milk, such as the somatic cell count (SCC) is an example of test used. Aiming to determine the...

Mastitis is the most important diseasesin milk cattle because it causes great disadvantages due to the production loss, expenses with medical service and remedy. Its etiology is diversified, but bacteria are the most common cause of the disease, especially Staphylococcus aureus, characterizing contagious mastitis. The disease causes problems to Pub...

The influence of pH on pyrrole electropolymerization on iron surfaces in malate aqueous medium was analyzed using different electrochemical techniques. The sodium sulfate solution was used as reference.Homogeneous and adherent PPy films were obtained at pH between 4 and 7 in 0.2M malic and 0.5M pyrrole solution by potentiostatic mode, while the PPy...

The electrochemical behaviour of sodium borohydride on a platinum electrode in the absence and presence of thiourea (TU) was investigated by cyclic voltammetry. In the absence of thiourea, several overlapping peaks associated with the hydrolysis of BH4− appear in the domain of hydrogen oxidation, i.e., in the potential range of −1.25 to −0.50 V ver...

The electrochemical behaviour of stainless steel electrode in 0.1M sodium saccharinate solution in the absence and the presence of the monomer were investigated using several electrochemical techniques such as cyclic voltammetry, galvanostatic and potentiostatic. The XPS analysis was used to study the elementary composition of the obtained polymer...

The electrochemical synthesis of polypyrrole (PPy) on copper and brass has been successfully achieved in sodium saccharinate and pyrrole aqueous medium. The synthesized coating under several electrochemical techniques such as cyclic voltammetry, galvanostatic and potentiostatic is homogeneous and adherent. PPy has been characterized by SEM, XPS, IR...

The electrochemical synthesis of polypyrrole on iron has been fruitfully achieved in aqueous medium of sodium saccharinate and pyrrole. The obtained coating under these conditions has been reached with cyclic voltammetry and chronomethods. The applied current density has a great effect on the thickness of PPy electrodeposited on iron electrodes. Th...

The electrosynthesis of polypyrrole (PPy) has been achieved on aluminum electrode in aqueous medium of 0.1 M saccharin sodium salt and 0.5 M pyrrole. Scanning electron microscopy shows that the PPy coating obtained in galvanostatic and potentiostatic modes starts with small islands at weak applied potentials or current densities. Moreover, energy d...

The electrodeposition of polypyrrole (PPy) on oxidizable metals such as aluminum and iron has been achieved in aqueous medium of saccharin and pyrrole. Scanning electron microscopy and X-ray photoelectron spectroscopy analysis reveal a good homogeneity of the obtained PPy film. The electrochemical synthesis of PPy coating has been achieved successf...

A single-step electropolymerization of pyrrole on zinc-coated steel electrodes in aqueous medium was investigated under several techniques (potentiodynamic, galvanostatic and potentiostatic modes) by using carboxylate salts, such as the citrate, the succinate, the oxalate, the malate and the tartrate. Adherent and homogeneous PPy coatings were obta...

The electrosynthesis of polypyrrole (PPy) on copper and brass (Cu–Zn alloy) electrodes was performed by anodic oxidation of pyrrole in a sodium tartrate (C4H4Na2O6 0.2 M) aqueous solution. The tartrate counter-ions slow the dissolution of the working electrode by leading to formation of a passivation layer on its surface, and pyrrole electropolymer...

The electropolymerization of thiophene on zinc and a zinc alloy has been achieved in nitrobenzene and dichloromethane in the presence of 0.1 M NBu 4PF6 supporting electrolyte and 0.5 M thiophene. The obtained polythiophene coating has been characterized by different microscopic and spectroscopic techniques. The micrographs performed by SEM have pro...

Electrosynthesis of polypyrrole (PPy) was carried out on galvanized steel in tartrate aqueous solution. During the polarization process, the tartrate counter-ion inhibits the electrode dissolution and the polymerization reaction takes place. The PPy coatings successfully obtained under different electrolysis techniques are very homogeneous and stro...

The effect of chemical composition of some fuel compounds: sodium tetrahydridoborate, boron hydride dimethylamine, lithium aluminium hydride, hydrazine, ethanolamine, ethanol and ethylenediamine, on the electrical response of a laboratorial membrane fuel cell have been studied in alkaline medium. The self-discharge, current-voltage and power curves...

This paper reports the study of ultrasonic effects on some physicochemical properties of electroplated copper on iron subtracts from an acid sulphate bath. The bath efficiency for three stirring processes has been also studied as a function of the time and the current density applied. The bath efficiency, the hardness, the brightness and the adhere...

Electropolymerization of pyrrole on zinc was investigated in sodium tartrate aqueous solution in order to obtain homogeneous and adherent polypyrrole films. The tartrate counter-anions inhibit the working electrode dissolution by leading to the formation of a passivation layer on its surface. Consequently, homogeneous and strongly adherent polypyrr...

A single-step electropolymerization of pyrrole (Py) on iron and steel surfaces has been performed in aqueous medium in the presence of malate (C4H4O52−) as supporting electrolyte. The malate counter-ion slows down the iron dissolution by leading to the formation of a passivation layer on the working electrode surface, and the Py electropolymerizati...

Electropolymerization of pyrrole on zinc–lead–silver (Zn–Pb–Ag) alloys was carried out in acetonitrile, nitrobenzene and propylene carbonate (PC) organic media in the presence of para-toluene sulfonate counter ions. Adherent and homogeneous PPy films have been obtained on zinc alloy electrodes (65% Zn, 10% Pb and 25% Ag) prealably treated with aque...

Homogeneous and strongly adherent polypyrrole (PPy) films were electrochemically synthesized on iron electrodes in sodium tartrate (Na2C4O6H4 0.2 M) aqueous solution. This one step pyrrole electropolymerization process has been successfully achieved under different electrochemical techniques, such as potentiodynamic, galvanostatic and potentiostati...

The electrodeposition of polypyrrole on zinc electrodes has been performed by anodic oxidation of pyrrole in acetonitrile, nitrobenzene and propylene carbonate media in the presence of para-toluene sulfonate counter-ions. When the zinc electrode has preferably undergone a chemical treatment by aqueous sodium sulfide solution, homogeneous and adhere...

The control of bovine brucellosis requires the differential serological diagnosis of the non-vaccinated animals and animals vaccinated with B. abortus-S-19. This is almost impossible using the classic serological diagnostic tests, such as seroagglutination in plate (SAP), seroagglutination in tube (SAT), and 2-mercaptoethanol (2Me). In this study,...