Liqin Cao

Liqin Cao
University of Tsukuba · International Institute for Integrative Sleep

Doctor of Philosophy

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62
Publications
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893
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Publications

Publications (62)
Article
Full-text available
The neural mechanisms of fear-associated thermoregulation remain unclear. Innate fear odor 2-methyl-2-thiazoline (2MT) elicits rapid hypothermia and elevated tail temperature, indicative of vasodilation-induced heat dissipation, in wild-type mice, but not in mice lacking Trpa1–the chemosensor for 2MT. Here we report that Trpa1 −/− mice show diminis...
Article
Full-text available
Thiazoline-related innate fear-eliciting compounds (tFOs) orchestrate hypothermia, hypometabolism, and anti-hypoxia, which enable survival in lethal hypoxic conditions. Here, we show that most of these effects are severely attenuated in transient receptor potential ankyrin 1 ( Trpa1 ) knockout mice. TFO-induced hypothermia involves the Trpa1 -media...
Preprint
Full-text available
Mammals normally maintain a constant body temperature irrespective of their environmental temperature. However, emotions such as fear can trigger acute changes in body temperature accompanying defensive behaviors to enhance survival in life-threatening conditions. The neural mechanisms of fear-associated thermoregulation remain unclear. Here, we fi...
Article
Full-text available
Sleep disturbances have been recognized as a core symptom of post-traumatic stress disorders (PTSD). However, the neural basis of PTSD-related sleep disturbances remains unclear. It has been challenging to establish the causality link between a specific brain region and traumatic stress-induced sleep abnormalities. Here, we found that single prolon...
Article
Full-text available
Sleep and wake have global effects on brain physiology, from molecular changes1-4 and neuronal activities to synaptic plasticity3-7. Sleep-wake homeostasis is maintained by the generation of a sleep need that accumulates during waking and dissipates during sleep8-11. Here we investigate the molecular basis of sleep need using quantitative phosphopr...
Article
Full-text available
Innate behaviors are genetically encoded, but their underlying molecular mechanisms remain largely unknown. Predator odor 2,4,5-trimethyl-3-thiazoline (TMT) and its potent analog 2-methyl-2-thiazoline (2MT) are believed to activate specific odorant receptors to elicit innate fear/defensive behaviors in naive mice. Here, we conduct a large-scale rec...
Article
Mitochondrial DNA segregation is one of the characteristic modes of mitochondrial inheritance in which the heteroplasmic state of mitochondrial DNA is transmitted to the next generation in variable proportions. To analyze mitochondrial DNA segregation, we produced a heteroplasmic mouse strain with interspecific mitochondrial DNA haplotypes, which c...
Chapter
Intravital microscopy is a powerful tool for discovery and analysis in cell biology, neurobiology, immunology and oncology and has increasingly become a method used in most biomedical research. Recently, advances in imaging system, fluorescence labeling and tissue immobilization techniques have made it possible to in vivo image subcellular structur...
Article
Full-text available
Intravital imaging of brain and bone marrow cells in the skull with subcellular resolution has revolutionized neurobiology, immunology and hematology. However, the application of this powerful technology in studies of abdominal organs has long been impeded by organ motion caused by breathing and heartbeat. Here we describe for the first time a simp...
Data
Comparison of videos of pancreatic tissue from GFP–LC3 mice acquired without (top row) and with (bottom row) the microstage device. GFP-positive pancreatic acinar cells (left, green). Vasculature (right, red, labeled with AngioSense 750 IVM) in the same image field. Both videos were acquired using the same image acquisition parameters and were cons...
Data
Videos of the GFP-LC3 mouse liver tissue acquired with the microstage device. This movie was extracted from Movie S2 (bottom row) by removing the frames with streak artifacts from the image stacks. Liver cells (left, green). Vasculature (right, red). Scale bar, 20 µm. (MOV)
Data
Time-lapse imaging of the GFP-LC3 mouse pancreas. Image series were recorded every 30 s, 12.5-min total duration. Pancreatic acinar cells (left, green). Vasculature (right, red). Scale bar, 20 µm. (MOV)
Data
Time-lapse imaging of the GFP-LC3 mouse liver. Image series were recorded every 60 s for the first 20 min followed by every 10 min up to a 120-min total duration. Liver cells (left, green). Vasculature (right, red). Scale bar, 20 µm. (MOV)
Data
Videos of the 3D reconstructions of GFP–LC3 mouse liver (left) and skeletal muscle (right) vasculatures. (MOV)
Data
Tissue stabilization for intravital imaging of mouse abdominal organs. (A, B) Stick-type objective (arrow) and microstage device (arrowhead). (C–E) Type I, type II and type III microstages were used to minimize tissue motion of the liver (C), kidney (D) and testis (E), respectively. (TIF)
Data
Videos of cellular processes in the pancreas shown in Figure 3. The yellow box represents the area where two GFP–LC3 positive dots exhibited distinct behaviors in an acinar cell (yellow arrow and yellow arrowhead). The magenta box specifies the location where an event that appeared similar to exocytosis (magenta arrow) took place. The white box sho...
Data
Liver tissue of GFP–LC3 mouse acquired without (top row) and with (bottom row) the microstage device. Liver cells (left, green). Vasculature (right, red). Both videos were acquired using the same image acquisition parameters and were constructed without image alignment and z-selection of individual planes. Scale bars, 20 µm. (MOV)
Data
Videos of the GFP-LC3 mouse liver tissue. This movie was constructed using the image series of Movie S3 after having applied the alignment procedure. Liver cells (left, green). Vasculature (right, red). Scale bar, 20 µm. (MOV)
Data
Videos of the pancreas of a GFP–LC3 mouse after 24 h starvation. Pancreatic acinar cells (left, green). Vasculature (right, red). Scale bar, 20 µm. (MOV)
Data
In vivo imaging of the pancreas of the mRFP–MBD-nls transgenic mouse. Highly methylated chromocenters were labeled by mRFP–MBD-nls in pancreatic acinar cells. The axial positions marked in each frame denote the focal plane relative to the starting position (tissue surface). Arrows depict nuclei whose chromocenters became well resolved when the foca...
Data
Correlation between plasmid copy values measured by qrt-PCR and their corresponding true copy values in samples containing plasmid DNA in the presence of a ρ0B82 cell. Plasmid DNA was quantified and serially diluted. Each dilution was added with a single ρ0B82 cell and underwent qrt-PCR amplification in triplicate using PCR conditions referred to i...
Article
Full-text available
In mammals, observations of rapid shifts in mitochondrial DNA (mtDNA) variants between generations have led to the creation of the bottleneck theory for the transmission of mtDNA. The bottleneck could be attributed to a marked decline of mtDNA content in germ cells giving rise to the next generation, to a small effective number of mtDNA segregation...
Article
Full-text available
Species of the mussel genus Mytilus possess maternally and paternally transmitted mitochondrial genomes. In the interbreeding taxa Mytilus edulis and M. galloprovincialis, several genomes of both types have been fully sequenced. The genome consists of the coding part (which, in addition to protein and RNA genes, contains several small noncoding seq...
Article
Full-text available
Several studies have shown that in vertebrate mtDNAs the nucleotide content at fourfold degenerate sites is well correlated with the site's time of exposure to the single-strand state, as predicted from the asymmetrical model of mtDNA replication. Here we examine whether the same explanation may hold for the regional variation in nucleotide content...
Article
Full-text available
Observations of rapid shifts in mitochondrial DNA (mtDNA) variants between generations prompted the creation of the bottleneck theory. A prevalent hypothesis is that a massive reduction in mtDNA content during early oogenesis leads to the bottleneck. To test this, we estimated the mtDNA copy number in single germline cells and in single somatic cel...
Article
Full-text available
Both the maternal (F-type) and paternal (M-type) mitochondrial genomes of the Mytilus species complex M. edulis/galloprovincialis contain a noncoding sequence between the l-rRNA and the tRNA(Tyr) genes, here called the large unassigned region (LUR). The LUR, which is shorter in M genomes, is capable of forming secondary structures and contains moti...
Article
Full-text available
In Mytilus, females carry predominantly maternal mitochondrial DNA (mtDNA) but males carry maternal mtDNA in their somatic tissues and paternal mtDNA in their gonads. This phenomenon, known as doubly uniparental inheritance (DUI) of mtDNA, presents a major departure from the uniparental transmission of organelle genomes. Eggs of Mytilus edulis from...
Article
In Mytilus, females carry predominantly maternal mitochondrial DNA (mtDNA) but males carry maternal mtDNA in their somatic tissues and paternal mtDNA in their gonads. This phenomenon, known as doubly uniparental inheritance (DUI) of mtDNA, presents a major departure from the uniparental transmission of organelle genomes. Eggs of Mytilus edulis from...
Article
Full-text available
Previous studies have shown that in most pair matings of Mytilus edulis, M. trossulus, and M. galloprovincialis there is a large sex-ratio bias in favor of either males or females. The degree of bias is a characteristic property of the female parent, as matings of the same female with different males produce the same sex ratio, but matings of the s...

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